2026-10-29T19:59:29Zhttps://munzur-rdlopen.rawdatalibrary.net/oai/request

oai:munzur-rdlopen.rawdatalibrary.net:munzur/13702026-10-28T15:28:43Zopenairecom_munzur_1col_munzur_2com_munzur_11col_munzur_162

A new lectin from Amanita vaginata mushroom with potent anti-inflammatory activitySeliman, Thoiba Mogahid HusseinOzsoy, NurtenAltiparmak-Ulbegi, GulsumAlp-Kavlo, HevidarArcan, Gulsah GamzeAkev, NuriyeCan, AyseAksoy-Sagirli, PinarAmanita vaginataAnti-inflammatoryChromatographyCOX-2iNOSLectinMushroomRAW 264.7 macrophageThis study used 80% ammonium sulphate fractional precipitation, followed by affinity chromatography to purify a new lectin (AVL) from the crude extract of the Amanita vaginata mushroom. The purification procedure achieved a 20% yield, a specific activity of 5120 HU/mg protein, and a 16-fold increase in purity. SDS-PAGE analysis showed a single band of 45 kDa. We assessed AVL's anti-inflammatory effect by measuring levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), induced by lipopolysaccharide (LPS), in RAW 264.7 macrophages. Western blot analysis showed that iNOS and COX-2 protein levels were barely detectable in the control groups, but markedly elevated in the LPS-treated groups. However, AVL treatment significantly inhibited the expression of both iNOS and COX-2 in LPS-stimulated cells. This effect was dose-dependent. These findings suggest that AVL's anti-inflammatory effects are mediated through the suppression of iNOS and COX-2 expression.TAYLOR & FRANCIS LTD2026Articlehttps://rdlopen.rawdatalibrary.net/r/munzur/1370https://doi.org/10.1080/14786419.2025.25302121478-6427enNatural product researchinfo:eu-repo/semantics/closedAccessinfo:eu-repo/semantics/articlehttps://munzur-rdlopen.rawdatalibrary.net/tr/items/munzur/1370