2026-10-29T22:18:06Zhttps://munzur-rdlopen.rawdatalibrary.net/oai/requestoai:munzur-rdlopen.rawdatalibrary.net:munzur/20612026-10-28T15:29:24Zopenairecom_munzur_1col_munzur_2com_munzur_8col_munzur_161Effect of pre-freezing on motility, viability and abnormality of Nile tilapia fish Oreochromis niloticus sperm post cryopreservationMuchlisin, Z.A.Hasibuan, R.R.Maulida, S.Eriani, K.Fadli, N.Muhammadar, A.A.Handayani, L.S.Suharman, I.Kocabas, M.Kocabas, F.K.cryoprotectantsDMSOegg yolkfish breedinsperm qualityNile tilapia Oreochromis niloticus is a popular freshwater fish that has been extensively and intensively cultured worldwide. However, cryopreservation of its sperm, especially pre-freezing procedure, has not been properly developed. Therefore, the study aimed to determine the best pre-freezing procedure for cryopreservation of Nile tilapia Oreochromis niloticus sperm. The completely randomized design with five treatments and four replications was employed in this study. The tested treatments were T1=4°C → 0°C →-4°C →-10°C →-79°C →-196°C, T2=4°C→ 0°C →-4°C →-10°C →-196°C, T3=4°C → 0°C →-4°C →-196°C, T4=4°C → 0°C →-196°C), and T5=4°C →-196°C, with a 10 min equilibration at each respective temperature. Furthermore, sperm was cryopreserved for two weeks in liquid nitrogen (-179oC). The results of the ANOVA test showed that pre-freezing had a significant effect on sperm motility, and viability (P<0.05), but had no considerable impact on sperm abnormality (P>0.05). Treatment T4 exhibited higher motility and viability, but these values were not significantly different from T3 and T5. Based on practical consideration, it was recommended to utilize the T5 pre-freezing procedures (4°C →-196°C) for cryopreservation of Nile tilapia sperm. Considering these results, Nile tilapia sperm can be directly cryopreserved into liquid nitrogen after equilibration at 4°C for 10 min.Polska Akademia Nauk2025Articlehttps://rdlopen.rawdatalibrary.net/r/munzur/2061https://doi.org/10.24425/pjvs.2025.1540241505-1773enPolish Journal of Veterinary Sciencesinfo:eu-repo/semantics/closedAccessinfo:eu-repo/semantics/articlehttps://munzur-rdlopen.rawdatalibrary.net/tr/items/munzur/2061