Sürdürülebilir Kalkınma Amaçları

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• MakaleYalnız künye2025Scopus SJRQ3/r/munzur/1328
  Production and Partial Characterisation of Bifunctional Cellulase Genes of Anaerobic Gut Fungus Orpinomyces sp. GMLF19

  Yazdic, F.C.; Akyol, I.; Ozkose, E.

  Abstract: Fiber-degrading properties of anaerobic gut fungus, Orpinomyces sp. GMLF19, were investigated when it was grown on various carbon sources, namely carboxymethylcellulose, barley, oat, corncob and wheat straw. Fungi were developed under anaerobic conditions, and carboxymethylcellulase (CMCase), lichenase, and xylanase activities were determined by using DNS method. Meanwhile, the decreasing order of the enzyme activity of Orpinomyces sp. GMLF19 was observed to be lichenase > CMCase > xylanase. Relative activities of these enzymes in the isolate GMLF19 were found to be the highest when the culture was grown for 5 days. Cellulase gene domain was reproduced from chromosomal DNA and transferred into Escherichia coli with the aid of pCT plasmid vector and CMCase, lichenase and xylanase activities were determined using DNS method. The gene showed relatively higher CMCase activity and moderate lichenase activity while no xylanase activity was observed suggesting that the gene is bi-functional. The cell-associated activity of overexpressed Cel19 enzyme (E. coli cells containing this transferred gene were designated as C19) was relatively higher than extracellular activity. It was also recorded that this gene has 2 dockerin domains and 1 catalytic domain with the length of 1.585 bp, and it coded 50.59 kDa size of the protein which belongs to glycosyl hydrolase family 6.

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• MakaleYalnız künye2023Scopus SJRQ3/r/munzur/1854
  Synthesis, Characterization and Biocompatibility of Cross-linked Dicarboxylic Acids from Waste Potato Peel Starch

  Paşa, S.; Gürler, N.; Erdoğan, Ö.; Cevik, O.

  Abstract: People have become conscious about recycling or reusing many things they use in the 21st century because diminishing vital resources make it necessary, both personally and socially. The evaluation of waste is also critical in terms of national economies. In this study, starch dicarboxylic compounds were synthesized using waste potato peels (WPP) in the presence of sodium hypophosphite monohydrate as a catalyst for the formation of the ester cross-links. Adipic acid, succinic acid, and glutaric acid were employed with waste potato starch (WPS) to obtain cross-linked samples: WPS-A, WPS-S, and WPS-G, respectively. The obtained compounds were characterized by attenuated total reflectance Fourier transform infrared (FTIR-ATR), scanning electron microscopy with energy-dispersive X-ray analysis (SEM-EDX), X-ray diffraction (XRD), and their cytotoxicity properties were investigated. The biocompatibility of the obtained WPS-dicarboxylic acid compounds against NIH-3T3 and L929 murine fibroblast cells with MTT assay was determined. Studies have shown that these compounds do not harm healthy cells and are found to be bioavailable.

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