Sürdürülebilir Kalkınma Amaçları

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• MakaleYayıncıda açık erişim2025Scopus SJRQ2/r/munzur/2267
  Determination of Toxic Effects of Copper in Navicula cryptocephala var. veneta by Biomarkers and Bioaccumulation Quantification

  Aydın, Ayşe Nur; Serdar, Osman; Ölçülü, Abdüllatif; Çimen, Işıl Canan Çiçek; Ak, Tuba Parlak; Derman, Taner; Sevim, Ayşegül; Yıldırım, Nuran Cikcikoğlu

  As a result of the increased use of metals such as copper (Cu), copper pollution in the environment is also increasing. In order to investigate the effect of copper mixed into the water environment and on aquatic biota, the microalga Navicula cryptocephala var. veneta was chosen as a model organism. N. cryptocephala var. veneta it was aimed to determine the accumulation and elimination amounts and some biomarker responses. Metal accumulation amounts in the organisms used in the study. The amount of metal accumulated in relation to the wet weight mass of the samples was determined using Atomic Absorption Spectophotometry (AAS) mass spectrometry. In the bioassays conducted within the scope of the study, lipid peroxidation (TBARS) and reduced glutathione (GSH) levels, superoxide dismutase (SOD) enzyme activity, glutathione peroxidase (GSH-Px) enzyme activity and catalase (CAT) enzyme activity were determined with an ELISA microplate reader at 24, 48, 72, 96 and 120 hours. According to the study data, it was observed that Cu metal accumulated in Navicula cryptocephala var. veneta and caused oxidative stress in the organism, affecting the organism by causing changes in TBARS, GSH levels and antioxidant enzymes SOD, CAT and GPx activities. Additionally, it was observed that different elimination levels occurred in the application groups.

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  AtıfScopus1·OpenAlex1

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• MakaleAçık Erişim2025Scopus SJRQ2/r/munzur/1451
  Determination of the Effect of Nanoparticle Copper on Navicula cryptocephala var. veneta by Biomarkers and Bioaccumulation Amounts

  Serdar, Osman; Aydın, Ayşe Nur; Ölçülü, Abdüllatif; Çiçek Çimen, Işıl Canan; Parlak Ak, Tuba; Derman, Taner; Pala, Ayşegül; Yıldırım, Nuran Cikcikoğlu

  Pollutants such as copper nanoparticles (CuNP), which form residues in the environment, especially in water environments, cause serious damage to the environment and the organisms living in the environment. In this study, Navicula cryptocephala var. veneta was selected as a model organism. The EC50 values of CuNP on Navicula cryptocephala var. veneta were determined as 4.94 mg\L and 3 different application concentrations were determined considering this ratio. The experiments were carried out in 3 replicates and samples were taken at 24 and 96 hours. At the end of 120 hours, samples were taken to determine the elimination values. CuNP accumulation amounts in the samples were measured by electrothermal atomic absorption spectrophotometer (ETAAS). For biomarker analysis, thiobarbituric acid (TBARS) and glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were performed using CAYMAN ELISA kit. According to the study data, as CuNP concentration and application time increased, the amount of accumulation in Navicula cryptocephala also increased. Increasing-decreasing changes were observed in elimination. CuNP caused oxidative stress in N. cryptocephala and caused changes in TBARS and GSH levels. In addition, it caused increases in SOD activities and statistically significant decreases (P<0.05) in CAT and GPx activities.

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  AtıfScopus2·WoS1

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• MakaleYalnız künye2023Scopus SJRQ4/r/munzur/1967
  Individual and Combined Toxicity of Polycyclic Aromatic Hydrocarbons Phenanthrene and Fluoranthene in Freshwater Amphipod Gammarus pulex (L., 1758) (Amphipoda: Gammaridae)

  Yildirim, Nuran Cikcikoglu; Serdar, Osman; Derman, Taner

  The combined toxicity of polycyclic aromatic hydrocarbons on freshwater amphipod Gammarus pulex was investigated. The 14-day LC50 values of fluoranthene, phenanthrene and their combination for G. pulex were determined as 5.88±0.23, 8.31±0.57 and 7.00±0.33 mg/L, respectively. To evaluate the single and combined toxicity, sublethal concentrations of fluoranthene, phenanthrene and their combination were exposed to G. pulex for 14 days. Acetylcholinesterase, glutathione-s-transferase and cytochrome P450 1A1 enzymes were measured as biomarkers. Acetylcholinesterase activities showed significant inhibition at the end of 7 and 14 days. No statistically significant change was observed in cytochrome P450 1A1 levels at the end of the 7th day, but a significant increase was detected at the end of the 14th day. A significant inhibition was observed in glutathione-s-transferase activities at the end of the 7th and 14th days. Statistically significant changes were observed in all parameters when the application times are compared. It was determined that G. pulex exposed to different concentrations of phenanthrene and fluoranthene individually and in combination caused significant changes in activites of the all measured enzymes. It was concluded that the cytochrome P450 1A1, glutathione-s-transferase and acetylcholinesterase enzymes in G. pulex are useful biochemical markers for the determination of polycyclic aromatic hydrocarbon toxicity.

  AtıfScopus3

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• Yüksek Lisans TeziAçık Erişim2021/r/munzur/7144
  Fenantren ve fluoranten poliaromatik hidrokarbonlarının tek ve kombine toksik etkilerinin Gammarus pulex'de belirlenmesi

  Taner Derman

  Polisiklik aromatik hidrokarbonlar (PAH'lar) havada, suda ve toprakta yaygın olarak bulunan organik bileşiklerdir. Günümüzde petrol türevi yakıtlardan ve tamamlanmamış yanma işlemlerinden elde edilen PAH'ların çevre üzerindeki miktarı giderek artmaktadır. Sucul ortamda omurgasızlar dahil suda yaşayan organizmalarda PAH toksisiteleri, biyobirikim özellikleri, endokrin bozulması ve dokuya özgü toksisite dahil olmak üzere PAH'ların toksisitesinin önemi ortaya çıkmıştır. Bu yönlerle ilgili olarak PAH'ların toksisitesinin belirlenmesi amacıyla Florantenler (Flu) ve Fenantren (Phe) PAH maddelerinin tek tek ve birlikte etkisinin sucul indikatör bir organizma olan Gammarus pulex üzerindeki nörotoksik, oksidatif stres ve maruz kalma etkisi nicel olarak belirlenmiştir. Bu amaçla öncelikle Flu, Phe ve Flu + Phe akut toksite (LC50) değeri sırasıyla 5,88 ± 0,23, 8,31 ± 0,57 ve 7,00 ± 0,33 mg/L olarak belirlenmiştir. G. pulex organizma bireyleri belirlenen LC50 değerine oranla sublethal konsantrasyonlarına 14 gün süreyle maruz bırakılarak nörotoksik (Asetilkolinesteraz), oksidatif stres (Glutatyon S-Transferaz) ve maruz kalma etkisi (Sitokrom P450) belirlenmiştir. G. pulex model canlısında asetilkolinesteraz (AChE) enzim düzeyleri kontrol grubu ile kıyaslandığında 7 ve 14 günün sonunda istatistiksel olarak anlamlı düzeyde inhibisyon gözlenmiştir. Sitokrom P450 (CYP1A1) maruz kalma etkisi kontrol grubu ile kıyaslandığında ise 7. günün sonunda tüm uygulama gruplarında istatistiksel olarak anlamlı düzeyde bir değişim gözlenmemiş olup 14. günün sonunda ise kontrol grubu ile kıyaslandığında CYP1A1 enzim düzeylerinde istatistiksel olarak anlamlı düzeyde artış olduğu saptanmıştır. Glutatyon S-Transferaz (GST) enzim aktiviteleri kontrol grubu ile kıyaslandığında 7 ve 14 gün maruz bırakma sonucunda gruplar arasında anlamlı düzeyde bir inhibisyon gözlenmiştir. Yapılan bu çalışma ile Phe ve Flu PAH'larına farklı konsantrasyonlarına tek tek ve kombine olarak maruz bırakılan G. pulex'te biyotransformasyon enzimleri olan (CYP1A1, GST) ve nörotoksisitenin bir biyobelirteçi olan (AChE) enzim seviyelerinde istatistiksel olarak önemli değişikliklere yol açtığı, kullanılan biyobelirteçlerden CYP1A1, GST ve AChE'nın yararlı birer biyobelirteç olduğu sonucuna varılmıştır.

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