Sürdürülebilir Kalkınma Amaçları

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• MakaleYalnız künye2024Scopus SJRQ3/r/munzur/1625
  EFFECT OF CRYOPROTECTANT AND CONCENTRATION ON THE SPERM QUALITY OF WALKING CATFISH, Clarias batrachus, POSTCRYOPRESERVATION

  Handayani, Luvi S.; Maulida, Siti; Rahayu, Sri R.; Razi, Nanda M.; Kocabas, Mehmet; Kocabas, Filiz K.; Wilkes, Martin; Siti-Azizah, Mohd N.; Eriani, Kartini; Fadli, Nur

  BACKGROUND: Walking catfish, Clarias batrachus is one of the native and most popular freshwater catfish species in Indonesia. However, cultivation faces challenges, particularly due to the scarcity of larvae resulting from underdeveloped breeding technologies. Cryopreservation is a method of storing sperm to maintain viability for a long period and support the breeding technology of the fish. Cryoprotectant, in this context, plays an important role in determining the success of sperm cryopreservation. OBJECTIVE: To determine the best type and concentration of cryoprotectant for cryopreservation of walking catfish sperm. MATERIALS AND METHODS: A total of five different types of cryoprotectants, namely DMSO, glycerol, ethyl glycol, ethanol, and methanol, were tested at four concentration levels namely 0%, 5%, 10%, 15%, and 20%, each with four replications. RESULTS: The type and concentration of cryoprotectant had a significant effect on sperm motility and viability (P<0.05). The best outcomes were obtained with 5% DMSO and ethyl glycol, 10% glycerol and methanol, as well as 15% ethanol. CONCLUSION: The highest motility and viability values were obtained with 5% DMSO, resulting in its recommendation for cryopreservation of walking catfish sperm.

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  AtıfScopus5·OpenAlex3

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• MakaleYalnız künye2023Scopus SJRQ3/r/munzur/4438
  IMPROVEMENT OF SPERM QUALITY OF THE DEPIK FISH, Rasbora tawarensis, AFTER CRYOPRESERVATION USING ANTIOXIDANT

  Muchlisin, Zainal A.; Afriani, Dian; Eriani, Kartini; Hasri, Iwan; Nur, Firman M.; Maulida, Siti; Handayani, Luvi S.; Fadli, Nur; Kocabas, Filiz K.; Siti-Azizah, Moh N.

  AtıfOpenAlex3

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• MakaleYalnız künye2023Scopus SJRQ3/r/munzur/2196
  EFFECT OF CRYOPROTECTANT AND PRE-FREEZING ON THE SPERM MOTILITY, VIABILITY AND FERTILITY OF GOLDFISH Carassius auratus (PISCES: CYPRINIDAE) POST SHORT-TERM CRYOPRESERVATION

  Nurlaili, Nurlaili; Eriani, Kartini; Salma, Itsnatani; Maulida, Siti; Rahayu, Sri Riska; Handayani, Luvi Syafrida; Kocabas, Filiz Kutluyer; Siti-Azizah, Mohd Nor; Wilkes, Martin; Muchlisin, Zainal Abidin

  BACKGRUND: Goldfish Carassius auratus is a popular ornamental fish extensively cultured worldwide. Sperm cryopreservation is a common fish breeding method that ensures sperm availability around the year. Studies on cryopreservation of goldfish sperm, especially on the suitability of cryoprotectant types and pre-freezing time, are scarcely available. OBJECTIVE: To determine the most suitable type of cryoprotectant and pre-freezing for the successful cryopreservation of goldfish sperm. MATERIALS AND METHODS: A completely randomized design with two factors was utilized in this study. The first factor is the type of cryoprotectants, which included methanol, ethanol, ethylene glycol, glycerol, and DMSO. The second is pre-freezing times of 10, 20, 30, and 40 min at each of the pre-freezing temperatures of 4 0C, -10 0C, and -79 0C, meaning that the total times for the ramping down of temperature were 30, 60, 90 and 120 min, respectively. The Ringer solution and 10% egg yolk were used as extender and extracellular cryoprotectant. The sperm was stored at -179 0C for 7 days. RESULTS: The ANOVA test showed that cryoprotectants and pre-freezing significantly affected the motility, viability, and fertility of goldfish sperm after freezing in liquid nitrogen for 7 days (P<0.05). Furthermore, 10% DMSO combined with 15% egg yolk with an pre-freezing time of 20 min can maintain sperm motility, viability, and fertility higher than other treatments, by 79%, 80%, and 33%, respectively. The agarose gel electrophoresis showed no DNA fragmentation in all samples, including fresh sperm. CONCLUSION: We conclude that 10% DMSO combined with 15% egg yolk and 20 min pre-freezing is the best treatment for goldfish sperm cryopreservation.

  AtıfScopus6·WoS5·OpenAlex5

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• MakaleYalnız künye2019Scopus SJRQ3/r/munzur/3638
  Effect of L-Tryptophan on Sperm Quality of Tigris Scraper (Capoeta umbla) (Pisces: Cyprinidae) after Cryopreservation

  Kutluyer, F.; Aksu, Ö.; Kocabaş, M.

  BACKGROUND: The aromatic amino acid L-tryptophan is a versatile molecule and needed for the biosynthesis of proteins. Due to metabolic functions, it has been widely used in research and clinical trials. OBJECTIVE: The present study aimed to test the usefulness of L-tryptophan as an extender of Tigris scraper (Capoeta umbla) sperm during cryopreservation. MATERIALS AND METHODS: Semen samples were diluted with a ratio of 1:3 (sperm: extender) and base extenders were supplemented with L-tryptophan (0 mM, 0.5 mM, 1 mM, 2 mM and 4 mM). Sperm motility and duration of post-thawed samples were assessed after the cryopreservation process. RESULTS: Our results revealed that motility rate and duration of sperm increased when the cryomedia was supplemented with L-tryptophan. On the other hand, an increase in the concentration of L-tryptophan in the extender caused a significant decrease (p<0.05) in the motility rate of Tigris scraper (C. umbla) sperm. The ideal results were obtained at 1 mM L-tryptophan; however, no motile sperm were shown in any samples when a concentration of 2 mM was used. CONCLUSION: The current study shows the importance of conducting further studies related to long-term storage and reproduction management.

  AtıfScopus12·WoS12

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• MakaleYalnız künye2016Scopus SJRQ3/r/munzur/4598
  CRYOPRESERVATION OF GOLDFISH (Carassius auratus) SPERMATOZOA: EFFECTS OF EXTENDER SUPPLEMENTED WITH TAURINE ON SPERM MOTILITY AND DNA DAMAGE

  Kutluyer, Filiz; Ögretmen, Fatih; Inanan, Burak Evren

  BACKGROUND: Amino acids, present in seminal plasma at high concentration, protect spermatozoa against cell damage during cryopreservation. OBJECTIVE: Experiments were designed to analyze the effect of semen extender supplemented with taurine on post-thawed sperm motility and duration, as well as DNA damage. MATERIALS AND METHODS: Extenders were supplemented with 1, 2 or 4 mM taurine. Semen samples were diluted at the ratio of 1:9 with the extenders. Diluted samples were aspirated into 0.25 ml French straws and 0.1 ml pellets. DNA damage was assessed with the comet assay after cryopreservation. RESULTS: The percentage and duration of sperm motility were significantly increased by taurine. Additionally, sperm motility and the motility period in pellets were higher than in straws. The best concentration of taurine was 4 mM, and the highest post-thaw motility rate (72.50 +/- 3.54%) and duration (17.50 +/- 0.71 s) were obtained from the extender with 4 mM in pellets. DNA damage was decreased after taurine supplementation. CONCLUSION: Pellets could be used for goldfish sperm cryopreservation. The addition of 4 mM taurine increases the post-thaw motility and decreases DNA damage on goldfish semen.

  AtıfWoS11

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• MakaleYalnız künye2015Scopus SJRQ3/r/munzur/4913
  EFFECTS OF SEMEN EXTENDER SUPPLEMENTED WITH L-METHIONINE AND PACKAGING METHODS (STRAWS AND PELLETS) ON POST-THAW GOLDFISH (Carassius auratus) SPERM QUALITY AND DNA DAMAGE

  Kutluyer, Filiz; Ögretmen, Fatih; Inanan, Burak Evren

  BACKGROUND: Amino acids protect spermatozoa against cell damage during cryopreservation due to have antioxidant property and found in seminal plasma at high concentration. OBJECTIVE: The aim of the present work was to analyse the effect of extender supplementation with L-methionine on post-thawed sperm motility, duration and DNA damage and also it was tested the feasibility of using straws and pellets method for the cryopreservation of goldfish (Carassius auratus) sperm. MATERIALS AND METHODS: Extenders were supplemented with different L-methionine concentrations of 1 mM; 1.5 mM; 3 mM; 6 mM. Semen samples diluted at the ratio of 1:9 by the extenders were subjected to cryopreservation. After dilution the semen was aspirated into 0.25 ml straws and 0.1 ml pellets, the straws and pellets were placed on the tray, frozen in nitrogen vapor and plunged into liquid nitrogen. DNA damage was determined with comet assay after cryopreservation.. RESULTS: Our results indicated that an increase in the concentration of L-methionine caused a significant increase in the motility rate and duration of sperm in goldfish (C. auratus) (p<0.05). In addition, duration and percentage of motility in pellets were higher than in straws. Comparing all concentrations of L-methionine, the best concentration of L-methionine was 1.5 mM. Highest post-thaw motility (45.00 +/- 7.07%) and duration of motility (17.00 +/- 0.71s) were obtained with the extender at concentration 1.5 mM in pellets. Addition of the extender with L-methionine was reduced DNA damage compared to control group. CONCLUSION: Consequently, pellets could be use for goldfish sperm cryopreservation and the tested amino acid affected the motility parameters, and semen extenders could be supplement with L-methionine.

  AtıfWoS8

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