Kandemir, F. M.; Sagliyan, A.; Ozkaraca, M.; Gunay, C.; Han, M. C.; Benzer, F.

This study examined the effects of orally given pomegranate seed extract (PSE) on healing of surgically induced full thickness skin wounds in terms of clinical, histopathological and biochemical aspects. A total of 48 healthy, adult male New Zealand rabbits were randomly divided in 2 equal experimental (PSE group) and control groups (C): one week prior the surgery, 100 mg/kg per day via gastric catheter pomegranate seed extract was orally given for 20 days to the assay animals. After one week, all rabitts were anaesthetized by intramuscular injection of xylazine HCl (5 mg/kg) and ketamine HCI (50 mg/kg) and after shaving and disinfection, a circular full thickness skin defect of 2.5 cm diameter was incised on the median line of the back area. Macroscopic and histopathological aspects as well as the tissue oxidant/antioxidant balance were determined on 6 rabbits from each group slaughtered at the 5th, 10th, 15th and 20th days post-surgery.

AtıfWoS9

İncele

Kandemir, F. M.; Benzer, E.; Ozkaraca, M.; Ceribasi, S.; Yildirim, N. Cikcikoglu; Ozdemir, N.

İncele

Sagliyan, A.; Benzer, E.; Kandemir, F. M.; Gunay, C.; Han, M. C.; Ozkaraca, M.

This study examined the effects of orally given grape seed extract (GSE) on healing of surgically induced full thickness skin wounds in terms of clinical, histopathological and biochemical aspects. A total of 48 healthy. adult male New Zealand rabbits were randomly divided in 2 equal experimental and control groups: one week prior the surgery, 260 mg/kg grape seed extract was orally given for 20 days to the assay animals. A circular full thickness skin wound (2.5 cm in diameter) was made in the back midline of all rabbits under anaesthesia then, macroscopic and histopathological aspects as well as the tissue oxidant/antioxidant balance were determined on 6 rabbits from each group slaughtered at the 5th, 10th, 15th and 20th days post-surgery. In treated rabbits, it was clinically observed that the wound contraction was significantly amplified and the histological processes of re-epithelisation, granulation tissue development, collagen accumulation and angiogenesis were markedly hastened whereas the inflammatory reaction and the ulcer disappeared more rapidly than in the not-treated controls. Furthermore, the maximal skin MDA formation observed on the 10th day was significantly depressed and the antioxidant catalase (CAT) and glutathione peroxidase (GSH-Px) activities remained significantly elevated until the 15th day compared to the controls. These results demonstrate the beneficial effects of treatment with GSE on skin wound healing in rabbits.

AtıfWoS4

İncele

Kandemir, F. M.; Issi, M.; Benzer, F.; Gül, Y.; Basbug, O.; Özdemir, N.

The aim of the present study was to investigate the plasma nitric oxide concentrations and the erythrocyte arginase activities in lambs with contagious ecthyma, which frequently occurs in lambs and goat kids. The study was conducted on 10 Akkaraman, 1-2 month old lambs with contagious ecthyma (the clinical diagnosis was based on the presence of typical cauliflower-like proliferative lesions in lips, commissum labials, nostrils and udders) and on 10 apparently healthy lambs (controls) from the same flock. Plasma NO concentrations and erythrocyte arginase activities were determined on all animals using the Griess method and the thiosemicarbazide diacetylmonoxime urea (TDMU) method, respectively. Compared to the control group, the mean plasma NO concentration was significantly increased in the diseased group while the mean arginase activity was markedly depressed. Abnormally high NO concentrations and low enzyme activities were observed in 9 and 10 diseased lambs, respectively. Additionally, a negative correlation was evidenced between the 2 biochemical parameters. These results show that this viral infection is associated with some perturbations in the arginine metabolism, leading preferentially to the synthesis of NO, which is presumed to exhibit some antiviral properties.

AtıfWoS7

İncele