Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2025/r/munzur/6205
  Determination of Oxidative Stress Responses in Dreissena polymorpha Exposed to Rare Earth Elements (Terbium, Lanthanum, Gadolinium and Praseodymium) with Temperature

  Serdar, Osman; Aydın, Ayşe Nur

  The continuous development of industry pushes people to search for new resources, and for this reason, the usage areas of Rare Earth Elements (REEs) are increasing day by day. Increasing concentrations of REEs, as a result of increased use, create pollution in the environment and harm living organisms. This pollution interacts with increasing temperature and causes more negative synergistic effects of the pollutant in the environment and in the living body. In this study, sublethal concentration values were determined by literature review and the concentration value was determined as 125 mg/L. In the present study tt was aimed to investigate some oxidative stress and antioxidant responses of Terbium, Lanthanum, Gadolinium and Praseodymium REEs in Dreissena polymorpha at 125 mg/L concentration at 3 different temperatures (16, 18, 20 0C) with biomarkers. For this purpose, 24 and 96 hour experimental trial design was created and 7 D. polymorpha were used in each trial group, and the application experiments were carried out with 3 replications. The samples at the end of the experimental phase were stored at -80 degrees Celsius until they were analyzed. In this study, Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) enzyme activities and glutathione (GSH) and Thiobarbituric acid (TBARS) level biomarker responses were determined by ELISA test microplate reader. CAYMAN brand SOD (Catalog No 706002), CAT Catalog No 707002) and GPx (Catalog No 703102), GSH (Catalog No 703002) and TBARS (Catalog No 10009055) were used in the study. SPSS 24.0 package program one-way ANOVA (Duncan 0.05) was used for the evaluation of biochemical analyzes. According to the study data, statistically significant decreases were observed in SOD and CAT activities in the oxidative stress responses of REEs on D. Polymorpha with increasing temperature, while there was no significant change in GPx activities. It was determined that there were increases in TBARS levels and decreases in GSH levels. It is thought that the temperature factor, application concentration and application time are effective in the formation of these changes. It can be said that temperature change and pollutants cause oxidative stress in organisms and cause cell damage.

  AtıfOpenAlex3·TR Dizin1

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• MakaleAçık Erişim2025/r/munzur/5718
  Differences in Quality Between Canned and Pouched Yellowfin Tuna (Thunnus albacares) Packed with Various Media

  Erol, Nida Demirtaş

  Canned and pouched tuna products are available in the markets in different liquid media such as brine, different oils and sauces. Limited information is provided about the pouched tuna products in different liquid media available in the Turkish market and the differences between them. The main purpose of this study is to evaluate the differences between two different tuna packaging methods using different packaging media. The pH values of CW (canned tuna in water) and PW (pouched tuna in water) were found to be lower than the others ((CO (canned tuna in olive oil), CS (canned tuna in sunflower oil), PO (pouched tuna in olive oil,) PS (pouched in sunflower oil)). It was observed that the TBA values of all groups were below the limits of developing objectionable odor/taste. The n-6/n-3 ratio was determined to be quite high in CS and PS. On the other hand, higher eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents were found in CW and PW compared to the others (CO, CS, PO, PS). It was determined that the tuna products were safe according to the heavy metal contents of arsenic, mercury, lead and cadmium levels. The L* values of all canned tuna (CO, CS, CW) were found to be significantly higher than those of pouched tuna (PO, PS, PW). The b* values of both CW and PW was found to be lower than the other groups. CW had the lowest overall quality, color and taste scores compared to the others. In this regard, pouched tuna products, especially PW, were recommended for health reasons.

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• MakaleAçık Erişim2024/r/munzur/6359
  Terbiyumun Pontastacus leptodactylus’ta ki Oksidatif Stres ve Antioksidan Yanıtlarının Belirlenmesi

  Serdar, Osman; Aydın, Ayşe Nur

  Terbiyum (Tb), su ortamında yüksek konsantrasyonlarda bulunduğunda sucul organizmalar için toksik hale gelebilen nadir toprak elementi (NTE)’dir. Tatlı su ekosistemindeki Tb toksisitesinin meydana getirdiği oksidatif stres sonuçlarıyla ilgili hala yeterince çalışmalar mevcut değildir. Bu çalışma ile Tb'nin oksidatif stres sonuçlarını değerlendirmek için tatlı su canlısı olan Pontastacus leptodactylus kullanılmıştır. Bu organizmada, 1, 5 ve 25 mg/L konsantrasyonlardaki Tb'nin 24 ve 96. saatte meydana getirdiği oksidatif stres ve antioksidan değişimleri süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px), katalaz (CAT) enzim aktiviteleri ile Tiyobarbitürik asit (TBARS) ve redükte glutatyon (GSH) seviyeleri belirlenmiştir. Sonuçlar ELISA kitleri kullanılarak tespit edilmiştir. Biyobelirteç analizlerin istatatistiksel değerlendirilmesinde SPSS 24.0 paket program tek-yönlü ANOVA (Duncan 0,05) testi kullanılmıştır. Kontrol grubu ile karşılaştırıldığında SOD aktivitesinde önemli bir değişim gözlemlenmezken (p>0,05) CAT ve GPx aktivitelerinde anlamlı azalmalar (p<0,05) gözlemlenmiştir; GSH ve TBARS seviyelerinde ise anlamlı (p<0,05) artışlar gözlemlenmiştir. Çalışma verileri dikkate alındığında canlı organizma vücuduna nüfuz eden Tb ksenobiyotiğinin organizma da oksidatif strese neden olduğu düşünülmektedir.

  AtıfOpenAlex4·TR Dizin3

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• MakaleAçık Erişim2024/r/munzur/6298
  Kocaavşar Deresi (Balıkesir)'nde Unio bruguierianus (Bourguignat, 1853) ve Unio pictorum (Linnaeus, 1758) Türlerinin İlk Kaydı ve Bazı Biyometrik Özellikleri

  Kocabaş, Mehmet; Kutluyer Kocabaş, Filiz; Akça, Ayşe; Atıl, Süleyman

  Bu çalışmada, Kocaavşar Deresi (Balıkesir)’nde (39°40’22”-27°35’34” Kuzey/Doğu) tatlı su midyeleri Unio bruguierianus ve Unio pictorum'un yaşadığı tespit edilmiştir. Ayrıca bu türlerin bazı biyometrik özelliklerini tanımlanmıştır. Bireylerdeki ağırlık (A) (g), kabuk uzunluğu (KU) (mm), kabuk genişliği (KG) (mm) ve kabuk yüksekliği (KY) ölçümleri yapılmıştır. Morfolojik ilişkiler, çok değişkenli istatistiksel analizlerle incelenmiştir. U. pictorum ve U. bruguierianus'un KU, KG, KY ve A ortalamaları sırasıyla şunlardır: 58,20±13,91 ve 75,34±8,08 mm (KU), 29,52±6,93 ve 39,89±7,04 mm (KG), 19,38±4,66 ve 26,33±3,79 mm (KY) ve 25,72±13,56 ve 46,15±9,59 g (A). Temel bileşenler analizi (TBA), her iki tür için KU, KG, KY ve A arasında kuvvetli bir ilişki olduğunu göstermiştir.

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• MakaleAçık Erişim2022/r/munzur/6551
  Determination of Letal Concentrations (LC50) of Cyfluthrın, Dimethoate Insecticides on Gammarus pulex (L., 1758)

  Serdar, Osman; Aydin, Rahmi; Aydın, Ayşe Nur

  In this study, Gammarus pulex (L., 1758) individuals obtained from Tunceli Munzur Stream were exposed to different concentrations of two insecticides containing cyfluthrin and dimethoate active ingredients, and LC50 values were determined from acute toxicity tests. During the study, parameters such as water temperature, pH, and dissolved oxygen were constantly controlled to ensure that they did not change. In this study, experiments were carried out by placing 0.5 liters of water in 1-liter glass aquariums. 10 G. pulex individuals were used for each concentration group. To determine the LC50 value, the mobility of the living things was observed and recorded in 24-hour periods. G. pulex, which lost its motility, was removed from the aquarium and excluded from the study. The study was carried out in 3 replications and the LC50 value for dimethoate was determined as 170.51 ± 8.15 µg/l, while the LC50 value for cyfluthrin was determined as 0.800 ± 0.12 µg/l.

  AtıfOpenAlex8·TR Dizin2

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• MakaleYalnız künye2021/r/munzur/6669
  Changes in the Some Biochemical Constituents in Fry of Common Carp (Cyprinus carpio) Exposed to Imidacloprid

  Yonar, M. Enis; Ispır, Ünal; Özcan, Mikail; Şeker, Engin

  This study was conducted to investigate the possible toxic effects of imidacloprid biochemical parameters of carp. The fish (weight 0,34±0,03 g, total length 2,97±0,21 cm) were subjected to 2,8 and 5,6 mg L-1 of Imidacloprid concentration for 4 and 7 days. Alterations were observed in glycogene, total protein, and total fat activities in the whole body of carp. No mortalities occurred in any group during the test. The glycogene and total protein levels in imidacloprid treated fishes were significantly (p&lt;0,05) lower than the control in all the concentrations. Total fat levels were higher in imidacloprid treatment groups, which were not significantly different. This study was conducted to investigate the possible toxic effects of imidacloprid biochemical parameters of carp.

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• MakaleAçık Erişim2020/r/munzur/6816
  Malathionun Gammarus pulex (Tatlı Su Amphipodu)’te Asetilkolinesteraz Aktivitesi Üzerine Akut Etkisi

  Serdar, Osman; Pala, Ayşegül; Aydin, Rahmi

  Bu çalışmada, organofosfat insektisit malathionun subletal konsantrasyonlarının Gammarus pulex'in AChE aktivitesi üzerine akut (24 ve 48 saat) etkisi incelenmiştir. Bu amaçla, G. pulex'e malathionun iki farklı sublethal konsantrasyonu (0.1 ve 0.2 mg l-1 ) 24 ve 48 saat boyunca uygulandı. 24 ve 48 saat malathion maruziyetinin sonunda G. pulex örnekleri alındı. Alınan örneklerde AChE enzim aktivitesi ve protein düzeyi belirlenerek, spesifik AChE enzim aktivitesi hesaplandı. Malathionunun sublethal konsantrasyonları G. pulex'te AChE aktivitesininin zamana bağlı artan inhibisyonuna neden oldu. 0.1 mg l-1 malathion konsantrasyonuna maruz kalan grupta, AChE'nin inhibisyonu, kontrol ile karşılaştırıldığında, 24 saatte %50 ve 48 saatte %74 olarak tespit edildi. Benzer şekilde, 0.2 mg l-1 malathion konsantrasyonuna maruz kalan grupta da kontrol ile karşılaştırıldığında sırasıyla 24 ve 48 saatte % 60 ve % 68 AChE inhibisyonu gözlendi. Sonuç olarak, G. pulex'in malathiona 24 ve 48 saat boyunca maruz kalması AChE aktivitesinin yüksek oranda inhibe edilmesine neden olmuştur. Ayrıca, sonuçlar G.pulex'teki % 74'e kadar AChE inhibisyon seviyelerinin akut ölüme neden olmadığını ve G. pulex'teki AChE aktivitesinin ölçümünün akut malathion maruziyetinin ve etkilerinin biyobelirteci olacağını göstermektedir.

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