Sürdürülebilir Kalkınma Amaçları

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• MakaleYalnız künye2020Scopus SJRQ2/r/munzur/3612
  Investigation on Microbial Biodeterioration of the Stone Monuments in Yildiz Technical University—Yildiz Campus—Istanbul—Turkey

  Özdemir, Aysun; Erguven, Gokhan Onder; Adar, Elanur; Nuhoglu, Yasar

  The stone samples of historical monuments around Yıldız Technical University Besiktas Campus were investigated using DNA extraction-PCR-DGGE methods, scanning electron microscopy (SEM), XRF, and other analytical methods to assess stone decay over the centuries. Microbial diversity was examined by classical cultivation and modern diagnostic methods besides modern analysis techniques. The number of the microorganisms in per gram of stone samples was calculated by microbial culture methods. SEM analysis showed that stone surfaces have too many pores, decaying pieces and microbial colony. It is put forth by XRF analysis that stone materials have some elements serving the growth of microorganisms. It was concluded that there is a close connection the stone structure and microbial growth, most likely mineralogical composition, hardness and porosity of stone. Cyanobacterial microorganisms lived on stone surfaces were also determined using denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments. It was revealed DNA-based molecular analysis of 16S rRNA that 23 bacterial/Cyanobacterial clones were inhabited to stone materials.

  AtıfScopus10·WoS10·OpenAlex11

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• MakaleAçık Erişim2017Scopus SJRQ2/r/munzur/3289
  The ability of Phanerochaete chrysosporium (ME446) on chemical oxygen demand remediation in submerged culture medium supplemented with malathion insecticide

  Erguven, Gokhan Onder; Yildirim, Numan; Adar, Elanur

  This present study aims to evaluating the efficiency of P. chrysosporium (P.c) to degrade the different concentrations of malathion (MLT) (50, 100 and 150 ppm) under agitated (130 rpm) submerged culture conditions via reduction of chemical oxygen demand (COD). We have previously screening the growth of fungus in sabouraud dextrose agar (SDA) medium for 6 days. We have also determined the laccase activity during degradation process for 15 days. According to our results the fungus has achieved 99.6, 98.8 and 98.7 % COD reduction at concentration of 50, 100 and 150 ppm respectively the end of the 15 days. The highest laccase activity (180 ± 5.69 U/L) was measured at concentration of 50 ppm the end of the 15 days and the lowest activity (36 ± 3.26) was determined at concentration of 150 ppm the end of the 15 days. The dried biomass of fungus was weighted at end of the 15 days and we have determined that malathion in culture medium reduced the biomass production compared the control group (SDB + P.c). Our results showed that, P. chrysosporium could be an effective bioremediation tool for treatment of malathion containing wastewater.

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  AtıfScopus11·WoS11·OpenAlex13

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