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- MakaleAçık Erişim2026Scopus SJRQ3Oxidative Stress Responses induced by Yttrium in Gammarus pulex at Increasing Temperatures
Aydın, Ayşe Nur; Serdar, Osman
In parallel with the increase in the need for raw materials used in industry, the area and amount of use of Rare Earth Elements are also increasing. Yttrium (Y), which is used as a raw material source, is thought to form residues in the environment. Gammarus pulex individuals were selected as a model organism to investigate the effects of Yttrium on the environment and aquatic organisms. G. pulexes were exposed to Y at different temperatures (16, 18, and 20 °C) and concentrations (25, 100, and 500 µg/L) for 96 hours and samples were taken at 24 and 96 hours for biochemical analyses. For biomarker analysis, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities, thiobarbituric acid (TBARS) and glutathione (GSH) levels were performed using CAYMAN ELISA kit. Study data revealed that SOD and CAT activities increased significantly with increasing temperature and exposure concentration, while GPx activity showed an irregular response; in contrast, no significant change was observed in TBARS levels at 16 °C, while the increase in TBARS and significant changes in GSH levels at 18 and 20 °C indicate that oxidative stress becomes more pronounced at high temperatures. Copyright Aydın,, A. N. & Serdar, O.
- MakaleAçık Erişim2025Determination of Toxic Effect of Gamma Cyhalothrin in Dreissena polymorpha by Some Biomarkers
Parlak Ak, Tuba; Aydın, Ayşe Nur; Serdar, Osman
In this study, the effects of pesticides on the freshwater mussel Dreissena polymorpha, a non-target organism, were aimed to be determined through biochemical responses. For this purpose, first, the acute toxicity value (LC50) of Gamma Cyhalothrin (GCH) pesticide on D. polymorpha was determined by standard static method and the LC50 value was calculated as 13.64 mg/L using probit analysis. Then, superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activities, malondialdehyde (MDA) and glutathione (GSH) level responses of D. polymorpha organism exposed to GCH sublethal concentrations for 24 and 96 hours were determined. In addition to biochemical biomarkers, histopathological responses were evaluated. In conclusion, this study demonstrated the abilities of GCH pesticides to induce oxidative stress. Moreover, MDA, GSH levels, SOD, CAT, GPx activities and histopathological responses could be used as an effective biomarker in D. polymorpha.
- MakaleAçık Erişim2025Determination of Oxidative Stress Responses in Dreissena polymorpha Exposed to Rare Earth Elements (Terbium, Lanthanum, Gadolinium and Praseodymium) with Temperature
Serdar, Osman; Aydın, Ayşe Nur
The continuous development of industry pushes people to search for new resources, and for this reason, the usage areas of Rare Earth Elements (REEs) are increasing day by day. Increasing concentrations of REEs, as a result of increased use, create pollution in the environment and harm living organisms. This pollution interacts with increasing temperature and causes more negative synergistic effects of the pollutant in the environment and in the living body. In this study, sublethal concentration values were determined by literature review and the concentration value was determined as 125 mg/L. In the present study tt was aimed to investigate some oxidative stress and antioxidant responses of Terbium, Lanthanum, Gadolinium and Praseodymium REEs in Dreissena polymorpha at 125 mg/L concentration at 3 different temperatures (16, 18, 20 0C) with biomarkers. For this purpose, 24 and 96 hour experimental trial design was created and 7 D. polymorpha were used in each trial group, and the application experiments were carried out with 3 replications. The samples at the end of the experimental phase were stored at -80 degrees Celsius until they were analyzed. In this study, Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) enzyme activities and glutathione (GSH) and Thiobarbituric acid (TBARS) level biomarker responses were determined by ELISA test microplate reader. CAYMAN brand SOD (Catalog No 706002), CAT Catalog No 707002) and GPx (Catalog No 703102), GSH (Catalog No 703002) and TBARS (Catalog No 10009055) were used in the study. SPSS 24.0 package program one-way ANOVA (Duncan 0.05) was used for the evaluation of biochemical analyzes. According to the study data, statistically significant decreases were observed in SOD and CAT activities in the oxidative stress responses of REEs on D. Polymorpha with increasing temperature, while there was no significant change in GPx activities. It was determined that there were increases in TBARS levels and decreases in GSH levels. It is thought that the temperature factor, application concentration and application time are effective in the formation of these changes. It can be said that temperature change and pollutants cause oxidative stress in organisms and cause cell damage.
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İncele - MakaleYayıncıda açık erişim2025Scopus SJRQ2Determination of Toxic Effects of Copper in Navicula cryptocephala var. veneta by Biomarkers and Bioaccumulation Quantification
Aydın, Ayşe Nur; Serdar, Osman; Ölçülü, Abdüllatif; Çimen, Işıl Canan Çiçek; Ak, Tuba Parlak; Derman, Taner; Sevim, Ayşegül; Yıldırım, Nuran Cikcikoğlu
As a result of the increased use of metals such as copper (Cu), copper pollution in the environment is also increasing. In order to investigate the effect of copper mixed into the water environment and on aquatic biota, the microalga Navicula cryptocephala var. veneta was chosen as a model organism. N. cryptocephala var. veneta it was aimed to determine the accumulation and elimination amounts and some biomarker responses. Metal accumulation amounts in the organisms used in the study. The amount of metal accumulated in relation to the wet weight mass of the samples was determined using Atomic Absorption Spectophotometry (AAS) mass spectrometry. In the bioassays conducted within the scope of the study, lipid peroxidation (TBARS) and reduced glutathione (GSH) levels, superoxide dismutase (SOD) enzyme activity, glutathione peroxidase (GSH-Px) enzyme activity and catalase (CAT) enzyme activity were determined with an ELISA microplate reader at 24, 48, 72, 96 and 120 hours. According to the study data, it was observed that Cu metal accumulated in Navicula cryptocephala var. veneta and caused oxidative stress in the organism, affecting the organism by causing changes in TBARS, GSH levels and antioxidant enzymes SOD, CAT and GPx activities. Additionally, it was observed that different elimination levels occurred in the application groups.
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İncele - MakaleYayıncıda açık erişim2025Scopus SJRQ4The effect of Dimethoate on oxidative stress and antioxidant responses of Pontastacus leptodactylus; [El efecto del dimetoato sobre el estrés oxidativo y las respuestas antioxidantes de Pontastacus leptodactylus]
Aydın, Ayşe Nur; Bulut, Hilal; Serdar, Osman
Dimethoate (DMT) pesticide is one of the chemicals used to protect some agricultural areas from harmful organisms. DMT residues released directly or indirectly to the environment cause serious problems in nature. DMT residues mixed with the aquatic environment adversely affect aquatic organisms and this effect is carried to humans through the food chain. In this study, oxidative stress responses induced by DMT pesticide in Pontastacus leptodactylus were investigated. For this purpose, oxidative stress and antioxidant parameters Thiobarbituric acid reactive substances (TBARS), Glutathione (GSH), Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) caused by dimethoate (DMT) pesticide in P. leptodactylus at 17.5, 35, and 70 mg·L-1 concentrations at 24 and 96 hours were investigated. Results were determined using ELISA kits. No significant difference was observed in GSH levels and SOD activities compared to control. Statistically significant differences were observed between decreases in CAT and GPx activities and increases in TBARS levels. SPSS 24.0 package program one–way ANOVA (Duncan 0.05) was used in the evaluation of biochemical analyzes. As a result, it was determined that DMT caused oxidative stress formation in P. leptodactylus and caused changes in enzyme activities.
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İncele - MakaleAçık Erişim2025Scopus SJRQ2Determination of the Effect of Nanoparticle Copper on Navicula cryptocephala var. veneta by Biomarkers and Bioaccumulation Amounts
Serdar, Osman; Aydın, Ayşe Nur; Ölçülü, Abdüllatif; Çiçek Çimen, Işıl Canan; Parlak Ak, Tuba; Derman, Taner; Pala, Ayşegül; Yıldırım, Nuran Cikcikoğlu
Pollutants such as copper nanoparticles (CuNP), which form residues in the environment, especially in water environments, cause serious damage to the environment and the organisms living in the environment. In this study, Navicula cryptocephala var. veneta was selected as a model organism. The EC50 values of CuNP on Navicula cryptocephala var. veneta were determined as 4.94 mg\L and 3 different application concentrations were determined considering this ratio. The experiments were carried out in 3 replicates and samples were taken at 24 and 96 hours. At the end of 120 hours, samples were taken to determine the elimination values. CuNP accumulation amounts in the samples were measured by electrothermal atomic absorption spectrophotometer (ETAAS). For biomarker analysis, thiobarbituric acid (TBARS) and glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were performed using CAYMAN ELISA kit. According to the study data, as CuNP concentration and application time increased, the amount of accumulation in Navicula cryptocephala also increased. Increasing-decreasing changes were observed in elimination. CuNP caused oxidative stress in N. cryptocephala and caused changes in TBARS and GSH levels. In addition, it caused increases in SOD activities and statistically significant decreases (P<0.05) in CAT and GPx activities.
AtıfScopus2WoS1İncele - MakaleAçık Erişim2024Terbiyumun Pontastacus leptodactylus’ta ki Oksidatif Stres ve Antioksidan Yanıtlarının Belirlenmesi
Serdar, Osman; Aydın, Ayşe Nur
Terbiyum (Tb), su ortamında yüksek konsantrasyonlarda bulunduğunda sucul organizmalar için toksik hale gelebilen nadir toprak elementi (NTE)’dir. Tatlı su ekosistemindeki Tb toksisitesinin meydana getirdiği oksidatif stres sonuçlarıyla ilgili hala yeterince çalışmalar mevcut değildir. Bu çalışma ile Tb'nin oksidatif stres sonuçlarını değerlendirmek için tatlı su canlısı olan Pontastacus leptodactylus kullanılmıştır. Bu organizmada, 1, 5 ve 25 mg/L konsantrasyonlardaki Tb'nin 24 ve 96. saatte meydana getirdiği oksidatif stres ve antioksidan değişimleri süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px), katalaz (CAT) enzim aktiviteleri ile Tiyobarbitürik asit (TBARS) ve redükte glutatyon (GSH) seviyeleri belirlenmiştir. Sonuçlar ELISA kitleri kullanılarak tespit edilmiştir. Biyobelirteç analizlerin istatatistiksel değerlendirilmesinde SPSS 24.0 paket program tek-yönlü ANOVA (Duncan 0,05) testi kullanılmıştır. Kontrol grubu ile karşılaştırıldığında SOD aktivitesinde önemli bir değişim gözlemlenmezken (p>0,05) CAT ve GPx aktivitelerinde anlamlı azalmalar (p<0,05) gözlemlenmiştir; GSH ve TBARS seviyelerinde ise anlamlı (p<0,05) artışlar gözlemlenmiştir. Çalışma verileri dikkate alındığında canlı organizma vücuduna nüfuz eden Tb ksenobiyotiğinin organizma da oksidatif strese neden olduğu düşünülmektedir.
AtıfOpenAlex4TR Dizin3İncele - MakaleAçık Erişim2024Effect of Probiotic on Copper Nanoparticle Accumulation in Dreissena polymorpha
Serdar, Osman; Aydın, Ayşe Nur
Materials with dimensions between 0.1 and 100 nm are called nanoparticle (NP) materials. In recent years, the usage areas and quantities of NP materials have increased in parallel with the development of the industry. The need and usage areas of heavy metals such as Cu have also expanded in NP sizes. All these developments have led to problems on the ecosystem that are becoming more difficult to compensate. In this study, Zebra Mussel (Dreissena polymorpha) was chosen as a model to investigate the effect of probiotics on CuNP heavy metal accumulation. The model organism was exposed to three different concentrations of CuNP (5, 10, 50 mg/L) with probiotics and directly for 24 and 96 hours. CuNP accumulation amounts in D. polymorpha tissues treated directly and with probiotics were compared. The amount of accumulation in the test organism directly exposed to CuNP was higher compared to the groups administered with probiotics, but a statistically significant difference (p<0.05) was found only in the treatment group with the highest 24-hour concentration (50 mg/L). As a result, according to the findings obtained from the study, it has been determined that probiotics have positive developmental effects on aquatic organisms, as well as beneficial in the elimination of their accumulation in the organism.
AtıfOpenAlex4TR Dizin1İncele - MakaleAçık Erişim2022Determination of Letal Concentrations (LC50) of Cyfluthrın, Dimethoate Insecticides on Gammarus pulex (L., 1758)
Serdar, Osman; Aydin, Rahmi; Aydın, Ayşe Nur
In this study, Gammarus pulex (L., 1758) individuals obtained from Tunceli Munzur Stream were exposed to different concentrations of two insecticides containing cyfluthrin and dimethoate active ingredients, and LC50 values were determined from acute toxicity tests. During the study, parameters such as water temperature, pH, and dissolved oxygen were constantly controlled to ensure that they did not change. In this study, experiments were carried out by placing 0.5 liters of water in 1-liter glass aquariums. 10 G. pulex individuals were used for each concentration group. To determine the LC50 value, the mobility of the living things was observed and recorded in 24-hour periods. G. pulex, which lost its motility, was removed from the aquarium and excluded from the study. The study was carried out in 3 replications and the LC50 value for dimethoate was determined as 170.51 ± 8.15 µg/l, while the LC50 value for cyfluthrin was determined as 0.800 ± 0.12 µg/l.
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