Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ3Determination of Oxidative Stress Responses Induced by the Combination of 4 Different Rare Earth Elements in Dreissena polymorpha; [Determinacija odgovorov na oksidativni stres, ki ga povzroča kombinacija 4 različnih redkih zemljinskih elementov v Dreissena polymorpha]
Serdar, Osman; Aydin, Ayşe Nur
Rare Earth Elements (REE), whose usage areas are increasing day by day, are increasing in the amount of mixing with the environment, causing changes in antioxidant enzyme activities by causing oxidative stress in living organisms. In this study, it was aimed to examine the oxidative stress responses induced by the mixture of 4 different REEs (terbium, gadolinium, Lanthanum, Praseodymium) in Dreissena polymorpha. For this purpose, sublethal concentration values were determined by literature review. Experimental application was carried out within 24 and 96 hours. In the analyzes performed to determine biomarker responses, samples taken from living organisms were weighed and homogenization processes were performed for the analysis of samples taken from the experimental groups, including the control group. After homogenization, samples were centrifuged at 4.000 rpm for 15 minutes. Supernatants were kept at -86 °C until measurements were made. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities, and glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) levels were determined using ELISA kits. Statistical analyses were performed using SPSS. One-way ANOVA (Duncan’s multiple range test; p < 0.05) was used for comparison of measured parameters among groups. As a result of the application, a decrease in CAT activity and GSH level and an increase in TBARS levels were observed after 96 hours compared to the control group, while no statistically significant difference was detected in SOD and GPx activities.
- MakaleAçık Erişim2026Scopus SJRQ4Oxidative Stress Response to Zinc Oxide, Copper Oxide and Iron (III) Oxide in Gammarus pulex; [Gammarus pulex'te Çinko Oksit, Bakır Oksit ve Demir (III) Oksit'e Karşı Oksidatif Stres Tepkisi]
Aydin, Ayşe Nur; Çiçek Çi̇Men, Işıl Canan; Serdar, Osman
Nanoparticles (NPs) are increasingly used across various fields of science and technology, including food science from crop harvesting to final food consumption. With their widespread application in daily life, the environmental effects of nanoparticles have become an important area of academic investigation. In this study, Gammarus pulex species was selected as research material/bioindicator species to investigate the effects of zinc oxide (ZnO), copper oxide (CuO), and iron(III) oxide (Fe2O3) on aquatic life. To conduct the experiments, separate setups were established for each of the nanoparticles (ZnO, CuO, and α-Fe2O3). Following ten organisms for each concentration group (10, 20, and 40 ppm) with corresponding control groups, exposures were performed for 24 and 96 hours. Samples were collected at both time points and biochemical analyses were conducted using homogenization procedures. The results were evaluated statistically. According to the findings, SOD activity increased significantly after exposure to ZnO, CuO, and Fe2O3. CAT activity did not show any change under ZnO NP exposure, whereas a decrease was observed under CuO and Fe2O3 NP exposures. However, a significant increase in TBARS levels and a significant increase in GSH levels were observed following nanoparticle exposure. Based on the study data, exposure to ZnO, CuO, and Fe2O3 appears to induce oxidative stress in G. pulex.
- MakaleYalnız künye2025Scopus SJRQ1Effect of light and noise pollution on oxidative stress and proximate composition in Dreissena polymorpha
Serdar, Osman; Demirtaş Erol, Nida; Aydin, Ayşe Nur
In this study, it was aimed to determine the effect of sound and light pollution on Zebra mussel (Dreissena polymorpha) by biomarker and nutritional composition. For this purpose, an experimental design was carried out in laboratory conditions where the model was exposed to live sound, light, and combined sound + light parameters for 30 min every hour over 96 h. Changes in the biomarkers superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, as well as glutathione (GSH) and thiobarbituric acid (TBARS) levels, were assessed in the model organism exposed to sound, light, and sound + light pollution. The changes in protein and fat amounts were determined. When the changes in biomarkers were examined statistically compared to the control, there was no significant decrease in SOD activity (p < 0.05), a significant increase in CAT activity in sound and sound+light, but a significant decrease in light (p < 0.05), no significant difference in GPx activity (p > 0.05). It was determined that there was a significant increase in GSH and TBARS levels in all three parameters. Significant changes were also observed in the proximate composition. As a result, exposure to sound, light and sound+light pollution caused an increase in oxidative damage and decreases in protein and lipid composition were detected.
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İncele - MakaleAçık Erişim2024Scopus SJRQ1Determination of oxidative stress responses caused by aluminum oxide (γ-Al2O3 and α-Al2O3) nanoparticles in Gammarus pulex
Serdar, Osman; Aydin, Ayşe Nur; Çimen, Isıl Canan Çiçek
The rapid growth in the use of aluminum oxide nanoparticles (Al2O3 NPs) in various fields such as medicine, pharmacy, cosmetics industries and engineering, and the fact that these NPs and their wastes mix with the aquatic environment and damage the aquatic ecosystem, affect the organisms in the water, enter the food chain and reach humans is a major problem is cause for concern. The aim of this study is to investigate the oxidative stress caused by two separate forms of aluminum oxide, γ-Al2O3 and α-Al2O3, in Gammarus pulex, which is a good indicator species, with biochemical parameters. For this purpose, G. pulex was exposed to different concentrations (0, 10, 20, 40 ppm) of γ-Al2O3 and α-Al2O3 separately. The experiments were carried out for 24 and 96 h by creating 3 repeated experimental groups consisting of 4 groups. For biomarker analysis, superoxide dismutase (SOD), catalase (CAT) activities and glutathione (GSH) and thiobarbituric acid (TBARS) levels were performed using an ELISA kit. As a result of the in experimental study, it was observed that both nanoparticles affected oxidative stress and antioxidant parameters after 96 h compared to the control group. Increases in SOD activity were observed, γ-Al2O3 caused a decrease in CAT activity at 24 h, and α- Al2O3 caused increases in CAT activity at 96 h. Decreases in GSH levels and increases in TBARS levels have been observed.
AtıfScopus14OpenAlex14
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