Sürdürülebilir Kalkınma Amaçları

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• MakaleYalnız künye2020Scopus SJRQ4/r/munzur/3632
  Spermatologic characteristics and sperm motility alterations caused by short-term copper exposure in Brook Trout Salvelinus fontinalis

  Kutluyer, Filiz; Kocabaş, Mehmet; Başçınar, Nadir

  Spermatozoa movement in many aquatic animals with external fertilization is directly related to the external aqueous environment. In particular, environmental toxic metals can cause harmful effects and impair sperm cell functions. The high concentrations of copper (Cu) may be harmful for aquatic animals, while low levels are required for metabolic interactions. Herein, we investigated the effect of different copper concentration [0 mg L−1 (Control),.5 mg L−1, 1 mg L–1, and 2 mg L−1] on spermatozoa of brook trout Salvelinus fontinalis. Motility and survival of sperm cells were assessed. Our results indicated that Cu exposure caused to an evident decrease in motility and longevity of spermatozoa with increasing concentrations (p <.05). Overall, we showed that Cu exposure can impair sperm motility of S. fontinalis. Our study suggested that sperm motility has the potential to be a sensitive bioindicator of freshwater environmental pollution.

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• MakaleAçık Erişim2019Scopus SJRQ2/r/munzur/3578
  Improvement of sperm motility of Oncorhynchus mykiss and Salvelinus fontinalis by L-tryptophan

  Kocabaş, Mehmet; Kutluyer, Filiz; Ertekin, Özlem; Aksu, Önder; Başçınar, Nadir

  Experiments were designed to test the influence of L-tryptophan supplementation of the extender on the sperm motility parameters and bacterial flora of brook trout (Salvelinus fontinalis) and rainbow trout (Oncorhynchus mykiss). The extender containing 0.3 M glucose in 10% Dimethyl sulfoxide (DMSO) was supplemented with 0, 0.5, 1, 2, and 4 mM L-tryptophan. Sperm samples stored for up to 12 days at 4°C for brook trout were motile while motility was not observed after six days of storage for rainbow trout. Diluted sperm samples were spread-plated onto Plate Count Agar (PCA) (Total Bacteria Count), Rose Bengal Agar (RBC) (Yeast-Mold Count), Violet Red Bile Dextrose (VRBD) (Enterobacteriaceae count) and Mannitol Salt Agar (MSA) (Micrococcus/Staphylococcus count) and counts were performed in duplicate and sampling occurred on day 0, 2, 4, 6, and 12 of cold storage. L-tryptophan supplementation of the extender yielded a positive effect, significantly prolonging sperm motility in S. fontinalis and O. mykiss compared to the control group (p < 0.05). The ideal results were obtained above a concentration of 0.5 mM for both species. Total bacteria count in fresh sperm (undiluted samples) was not detectable and not detected in sperm samples treated with different L-tryptophan concentrations. Yeast-mold, Enterobacteriaceae and Micrococcus/Staphylococcus were not detected from fresh and treated sperm brook trout and rainbow trout. On the basis of the results, L-tryptophan-based extender is useful for maintaining sperm quality in short-term storage. L-tryptophan had a negative effect on the bacterial flora. The results of the current study encourages further studies related to long-term storage and reproduction management. Abbreviations: DMSO: dimethyl sulfoxide; PCA: plate count agar; RBC: rose Bengal agar; VRBD: violet red bile dextrose; MSA: mannitol salt agar; SCA: sperm class analyzer; CFU: colony-forming unit.

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  AtıfScopus13·WoS14·OpenAlex13

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