Sevindik, Emre; Güner, Özal; Yolcu, Seher; Başköse, İsa; Paksoy, Mehmet Yavuz; Khadivi, Ali; Tunç, Yazgan; Yaman, Mehmet

In this study, the phylogenetic relationship of four species of Dianthus genus which are endemic and distributed in Türkiye, including D. zederbaueri Vierch, D. varankii Hamzaoğlu & Koç, D. halisdemirii Hamzaoğlu & Koç, and D. goekayi Kaynak, was determined using nuclear DNA (nrDNA) internal transcribed spacer (ITS) and chloroplast DNA (cpDNA) rbcL regions. After sampling from the plant, DNA was extracted and the ITS (ITS4-ITS5A) and rbcL (rbcLaF-rbcLaR) primers were used to perform PCR amplification. Sequencer 5.4.6. was used to edit the sequences. MEGA 11 software was used to determine the nucleotide ratios, genetic distance matrix, and π = nucleotide diversity. Some species of Dianthus available in the National Center for Biotechnology Information (NCBI) were used to construct maximum likelihood (ML) phylogenetic trees. As a result, based on the ITS analysis π = nucleotide diversity was determined as: 0.001543, and the result of the rbcL analysis π = nucleotide diversity was determined as: 0.000000. In the ITS results, D. halisdemirii, D. superbus, and D. varankii appeared together, while D. zederbaueri and D. aculeatus appeared together. The rbcL results showed that D. zederbaueri and D. varankii were closely related, but the relationship between D. halisdemirii and D. goekayi could not be determined. Furthermore, in the phylogenetic tree constructed by taking the ITS and rbcL sequences of Dianthus, Gypsophila, Silene, Arenaria, Saponaria, Cerastium, Stellaria, Petrorhagia, Minuartia, Agrostemma, and Polycarpon species from NCBI using ITS and rbcL sequences, our endemic Dianthus species appeared in the same group with Petrorhagia, Gypsophila, and Saponaria species. To conclude, our ITS and rbcL results were consistent in determining the phylogenetic relationships of our Dianthus species with other species.

AtıfScopus1

İncele

Paksoy, Mehmet Yavuz; Başköse, İsa

Bu çalışma 2018-2022 yılları arasında Tunceli il sınırlarında yer alan Pülümür ilçesi florasına katkı sağlamak amacıyla yapılmıştır. Yapılan bu araştırmada toplanan bitki-lerin teşhis edilmesi sonucunda çalışma sahasında 89 familya ve 433 cinse ait 957 taksonun bulunduğu tespit edilmiştir. Bu taksonlardan 115’i endemiktir ve endemizm oranı %12’ dir. Araştırma sonucunda 304 taksonun Pülümür için yeni kayıt olduğu be-lirlenmiştir. Bitkilerin fitocoğrafik dağılımları sırasıyla; 337’si (%35.2) İran-Turan; 85’i (%8.8) Avrupa-Sibirya; 46’sı (%4.8) Akdeniz; 489’u (%51.2) ise Geniş yayılışlı ve Bilinmeyen olduğu görülmektedir.

İncele

Paksoy, Mehmet Y.; Sevİndİk, Emre; Başköse, İsa

In this study, we performed DNA barcoding and phylogenetic analysis using one nuclear (ITS) and two chloroplast DNA regions (matK and rbcL) of endemic Astragalus nezaketiae A. Duran & Aytaçand Vicia alpestris Stev. subsp. hypoleuca (Boiss.) Davis taxa in Turkey. PCR reactions were performed using universal primers. Sequences of the PCR products were edited using BioEdit and FinchTV software and contigs were obtained. All contigs were Blasted at NCBI and similarities were analysed. Using the MEGA 6.0 program, maximum likelihood trees were constructed including some sequences retrieved from NCBI. For Astragalus nezaketiae; in the ITS analysis, Astragalus nezaketiae appeared separately from other species, and for matK, Astragalus nezaketiae appeared together with Astragalus cicer L. However, rbcL tree was polytomic. For Vicia alpestris subsp. hypoleuca; in ITS, rbcL and matK results Vicia alpestris subsp. hypoleuca were found together with Vicia cracca L., Vicia benghalensis L. and Vicia villosa Roth species. Analysis of the combined data revealed similar results with all barcode regions for Vicia alpestris subsp. hypoleuca while different phylogenetic results were obtained for Astragalus nezaketiae.

AtıfScopus3·WoS2·OpenAlex3

İncele

Paksoy, Mehmet Yavuz; Başköse, İsa; Savran, Ahmet

This study was carried out to determine the flora of Nigde University Campus and Akkaya Dam Lake environments (Nigde). As a result of the examination of 1000 plants specimens collected from the area, between 2005- 2007 and 2010-2011, 405 taxa belong to 74 families and 262 genera were determined. One taxa belong to Pteridophyta, 404 taxa belong to Spermatophyta divisio. Gymnospermae are represented by five taxa and Angiospermae are represented by 399 taxa. The numbers of taxa belong to Dicotyledonae and Monocotyledonae are follows respectively 441 and 58. In addition to, 27 taxa are new records for C5 Grid square. The 33 of the total taxa are endemic to Turkey and the rate of endemism is 8.1 %. The phytogeographical regions are represented as follows; Irano-Turanian 21.0 %, Mediterranean 6.4 % and Euro-Siberian 6.2 %.

AtıfTR Dizin9

İncele