Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYayıncıda açık erişim2025Scopus SJRQ2Determination of Toxic Effects of Copper in Navicula cryptocephala var. veneta by Biomarkers and Bioaccumulation Quantification
Aydın, Ayşe Nur; Serdar, Osman; Ölçülü, Abdüllatif; Çimen, Işıl Canan Çiçek; Ak, Tuba Parlak; Derman, Taner; Sevim, Ayşegül; Yıldırım, Nuran Cikcikoğlu
As a result of the increased use of metals such as copper (Cu), copper pollution in the environment is also increasing. In order to investigate the effect of copper mixed into the water environment and on aquatic biota, the microalga Navicula cryptocephala var. veneta was chosen as a model organism. N. cryptocephala var. veneta it was aimed to determine the accumulation and elimination amounts and some biomarker responses. Metal accumulation amounts in the organisms used in the study. The amount of metal accumulated in relation to the wet weight mass of the samples was determined using Atomic Absorption Spectophotometry (AAS) mass spectrometry. In the bioassays conducted within the scope of the study, lipid peroxidation (TBARS) and reduced glutathione (GSH) levels, superoxide dismutase (SOD) enzyme activity, glutathione peroxidase (GSH-Px) enzyme activity and catalase (CAT) enzyme activity were determined with an ELISA microplate reader at 24, 48, 72, 96 and 120 hours. According to the study data, it was observed that Cu metal accumulated in Navicula cryptocephala var. veneta and caused oxidative stress in the organism, affecting the organism by causing changes in TBARS, GSH levels and antioxidant enzymes SOD, CAT and GPx activities. Additionally, it was observed that different elimination levels occurred in the application groups.
AtıfScopus1OpenAlex1
İncele - MakaleAçık Erişim2025Scopus SJRQ2Determination of the Effect of Nanoparticle Copper on Navicula cryptocephala var. veneta by Biomarkers and Bioaccumulation Amounts
Serdar, Osman; Aydın, Ayşe Nur; Ölçülü, Abdüllatif; Çiçek Çimen, Işıl Canan; Parlak Ak, Tuba; Derman, Taner; Pala, Ayşegül; Yıldırım, Nuran Cikcikoğlu
Pollutants such as copper nanoparticles (CuNP), which form residues in the environment, especially in water environments, cause serious damage to the environment and the organisms living in the environment. In this study, Navicula cryptocephala var. veneta was selected as a model organism. The EC50 values of CuNP on Navicula cryptocephala var. veneta were determined as 4.94 mg\L and 3 different application concentrations were determined considering this ratio. The experiments were carried out in 3 replicates and samples were taken at 24 and 96 hours. At the end of 120 hours, samples were taken to determine the elimination values. CuNP accumulation amounts in the samples were measured by electrothermal atomic absorption spectrophotometer (ETAAS). For biomarker analysis, thiobarbituric acid (TBARS) and glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were performed using CAYMAN ELISA kit. According to the study data, as CuNP concentration and application time increased, the amount of accumulation in Navicula cryptocephala also increased. Increasing-decreasing changes were observed in elimination. CuNP caused oxidative stress in N. cryptocephala and caused changes in TBARS and GSH levels. In addition, it caused increases in SOD activities and statistically significant decreases (P<0.05) in CAT and GPx activities.
AtıfScopus2WoS1
İncele - MakaleYalnız künye2023Scopus SJRQ4Individual and Combined Toxicity of Polycyclic Aromatic Hydrocarbons Phenanthrene and Fluoranthene in Freshwater Amphipod Gammarus pulex (L., 1758) (Amphipoda: Gammaridae)
Yildirim, Nuran Cikcikoglu; Serdar, Osman; Derman, Taner
The combined toxicity of polycyclic aromatic hydrocarbons on freshwater amphipod Gammarus pulex was investigated. The 14-day LC50 values of fluoranthene, phenanthrene and their combination for G. pulex were determined as 5.88±0.23, 8.31±0.57 and 7.00±0.33 mg/L, respectively. To evaluate the single and combined toxicity, sublethal concentrations of fluoranthene, phenanthrene and their combination were exposed to G. pulex for 14 days. Acetylcholinesterase, glutathione-s-transferase and cytochrome P450 1A1 enzymes were measured as biomarkers. Acetylcholinesterase activities showed significant inhibition at the end of 7 and 14 days. No statistically significant change was observed in cytochrome P450 1A1 levels at the end of the 7th day, but a significant increase was detected at the end of the 14th day. A significant inhibition was observed in glutathione-s-transferase activities at the end of the 7th and 14th days. Statistically significant changes were observed in all parameters when the application times are compared. It was determined that G. pulex exposed to different concentrations of phenanthrene and fluoranthene individually and in combination caused significant changes in activites of the all measured enzymes. It was concluded that the cytochrome P450 1A1, glutathione-s-transferase and acetylcholinesterase enzymes in G. pulex are useful biochemical markers for the determination of polycyclic aromatic hydrocarbon toxicity.
AtıfScopus3İncele