Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ2The Positive Effect of Antioxidants in Maintaining the Post-Cryopreserved Sperm Quality of Rainbow Trout Oncorhynchus mykiss (Walbaum, 1792)
Handayani, Luvi S.; Rahayu, Sri R.; Razi, Nanda M.; Pane, Elya P.; Kocabas, Mehmet; Kocabas, Filiz Kutluyer; Hafizuddin, Hafizuddin; Fadli, Nur; Sulistiono, Sulistiono; Muchlisin, Zainal A.
Rainbow trout aquaculture is challenging due to the restricted spawning seasons. Sperm cryopreservation technology can help ensure a successful spawning season, but its effectiveness is restricted by oxidative stress during freezing and thawing, which reduces the availability of male gametes. This study aimed to evaluate the effect of several types and concentrations of antioxidants on post-cryopreservation rainbow trout sperm quality. Subsequently, a factorial completely randomized design with three replications was applied, testing four types of antioxidants Ascorbic acid, Myo-inositol, Butylated hydroxytoluene (BHT), and Alpha-tocopherol at concentrations of 0, 20, 40, and 60 mM, respectively. The evaluation parameters included motility rate, sperm lifespan, and sperm motion characteristics using the Computer Assisted Sperm Analysis system. The results showed that the type and concentration of antioxidants significantly affected sperm motility and sperm lifespan (P<0.05). The treatment method carried out using 40 mM Ascorbic acid and 20 mM BHT led to the highest sperm motility of 46.67% and sperm lifespan of 34 seconds. Although both antioxidants showed similar effectiveness, BHT at a concentration of 20 mM is more recommended due to its lower toxicity and technical as well as economic efficiency considerations.
- MakaleYayıncıda açık erişim2025Scopus SJRQ3EFFECT OF ANTIOXIDANT AND ITS CONCENTRATION ON THE SPERM QUALITY OF WALKING CATFISH Clarias batrachus POST CRYOPRESERVATION
Handayani, Luvi Syafrida; Rahayu, Sri Riska; Razi, Nanda Muhammad; Kocabas, Mehmet; Kocabas, Filiz Kutluyer; Tian, Changxu; Hafizuddin, Hafizuddin; Fadli, Nur; Muchlisin, Zainal Abidin
The walking catfish Clarias batrachus is popular and affordable in Southeast Asian countries. The development of breeding technology for this species is ongoing, with sperm cryopreservation being a key aspect of study that supports its breeding program. Cryopreservation is an essential method in fish breeding, particularly for sustaining breeding initiatives that supply larvae throughout the years. However, this process can lead to oxidative stress, which may decrease sperm quality due to structural and physiological damage, including DNA fragmentation. Adding an antioxidant to the freezing medium could help mitigate these negative effects and maintain sperm quality. This study aims to evaluate the effectiveness of various types and concentrations of antioxidant on the quality of C. batrachus sperm post-cryopreservation. Four types of antioxidants were tested: ascorbic acid, Myoinositol, alpha-tocopherol, and Butylated Hydroxytoluene, each at concentrations of 0, 20, 40, and 60 mg/L. Ringer solution, 5% DMSO and 5% egg yolk were used as extender and cryoprotectants, respectively. Sperm samples were stored in liquid nitrogen for 2 weeks, after which sperm quality was analyzed. The results showed that both the type and concentration of antioxidant had a significant effect (p<0.05) on all measured sperm quality parameters. Ascorbic acid at a concentration of 40 mg/L produced the best results, achieving 62.33% motility, 66.00% viability, and 61.67% fertility. Electrophoresis analysis showed no DNA fragmentation in any of the treatments, indicating that the genetic structure of sperm remained intact. Ascorbic acid at 40 mg/L is the most effective antioxidant for cryopreservation of sperm C. batrachus.
- MakaleYalnız künye2025Scopus SJRQ4Induced breeding and seed production of seurukan fish (Osteochilus jeruk) using synthetic reproductive hormones
Muchlisin, Zainal Abidin; Tasnarizki, Dwimutia; Risky, Muhammad; Diani, Diani; Naurah, Aufa; Dewi, Cut Dara; Maulida, Siti; Razi, Nanda Muhamamd; Rahayu, Sri Riska; Handayani, Luvi Syafrida
The commercial aquaculture of seurukan fish (Osteochilus jeruk) is common in Indonesia; however, seed availability from the wild is limited. Therefore, induced breeding technology in seurukan fish using synthetic reproductive hormones is crucial. This study evaluated the efficacy of five different types of synthetic reproductive hormones and their corresponding doses on the reproductive performance of seurukan fish in captivity. The methodology consisted of two experiments; first, four types of synthetic reproductive hormones were tested: ovaprim, ovaspec, human chorionic gonadotropin (HCG), and ovalumon. Second, several doses of ovaprim hormone were stested with doses of 0.25, 0.50, 0.75, and 1.0 ml kg-1 body weight. The results showed that ovaprim, ovaspec, and HCG successfully induced ovulation and spawning in seurukan fish. The hormones significantly affected the latency period, number of eggs released, fertilization, and survival rates (P<0.05), but did not significantly affect the hatching rate and survival of larvae (P>0.05). Ovaprim produced better egg quality compared to ovaspec and HCG. In the second experiment, the results showed a significant difference in the latency period and egg size (P<0.05) of fish treated with varying doses of ovaprim hormone. The hormone doses did not significantly affect the number of eggs, hatching rate, fertilization rate, survival rate, or larval survival (P > 0.05). The dose of 0.5 ml kg-1 BW of ovaprim produced the best results. Therefore, a dose of 0.5 ml kg-1 BW was recommended as an effective dose for induced spawning and seed production of seurukan fish.
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İncele - MakaleAçık Erişim2024Scopus SJRQ4Effect of Sperm Extender and Dilution Ratio on the Sperm Motility, Fertility, and Hatching Rates of Depik Fish Rasbora Tawarensis (Pisces: Cyprinidae) Eggs
Muchlisin, Zainal Abidin; Aldila, Dhea Febby; Sarah, Putri Inten; Eriani, Kartini; Hasri, Iwan; Fadli, Nur; Muhammadar, Abdullah Abbas; Handayani, Luvi Syafrida; Maulida, Siti; Siti-Azizah, Mohd Nor
There is no known artificial breeding method for depik fish Rasbora tawarensis. Sperm extender and the dilution ratio are crucial factors in the artificial breeding of fish. The current study aimed to assess the effect of several common fish sperm extenders and its dilution ratio on sperm characteristics and fertilization of depik fish eggs. Two series of experiments were performed to find the best extender and its dilution ratio. In the first experiment, five types of extenders were tested: Ringer's solution, physiological solution, coconut water, sugarcane water, and roomie water. The second experiment studied four dilution levels of sperm and extender (v/v): 1:20, 1:30, 1:40, and 1:50. Every treatment was conducted in four replicates and the experiments were conducted consequently so that the findings in the first experiment were applied in the second. The first experiment showed that Ringer's is the best extender for depik sperm with sperm motility, fertilization and hatching rates of 71.00%, 69.30%, and 53.66%, respectively; and the second experiment revealed that Ringer's at dilution ratio 1:40 gave higher sperm motility, fertilization, and hatching rates of 74.66, 70.33, and 59.00%, respectively. In conclusion, Ringer's solution was the most favorable extender for sperm which yielded best fertilization results at 1:40 dilution ratio (sperm:extender, v/v) in depik fish.
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İncele - MakaleYalnız künye2023Scopus SJRQ3EFFECT OF CRYOPROTECTANT AND PRE-FREEZING ON THE SPERM MOTILITY, VIABILITY AND FERTILITY OF GOLDFISH Carassius auratus (PISCES: CYPRINIDAE) POST SHORT-TERM CRYOPRESERVATION
Nurlaili, Nurlaili; Eriani, Kartini; Salma, Itsnatani; Maulida, Siti; Rahayu, Sri Riska; Handayani, Luvi Syafrida; Kocabas, Filiz Kutluyer; Siti-Azizah, Mohd Nor; Wilkes, Martin; Muchlisin, Zainal Abidin
BACKGRUND: Goldfish Carassius auratus is a popular ornamental fish extensively cultured worldwide. Sperm cryopreservation is a common fish breeding method that ensures sperm availability around the year. Studies on cryopreservation of goldfish sperm, especially on the suitability of cryoprotectant types and pre-freezing time, are scarcely available. OBJECTIVE: To determine the most suitable type of cryoprotectant and pre-freezing for the successful cryopreservation of goldfish sperm. MATERIALS AND METHODS: A completely randomized design with two factors was utilized in this study. The first factor is the type of cryoprotectants, which included methanol, ethanol, ethylene glycol, glycerol, and DMSO. The second is pre-freezing times of 10, 20, 30, and 40 min at each of the pre-freezing temperatures of 4 0C, -10 0C, and -79 0C, meaning that the total times for the ramping down of temperature were 30, 60, 90 and 120 min, respectively. The Ringer solution and 10% egg yolk were used as extender and extracellular cryoprotectant. The sperm was stored at -179 0C for 7 days. RESULTS: The ANOVA test showed that cryoprotectants and pre-freezing significantly affected the motility, viability, and fertility of goldfish sperm after freezing in liquid nitrogen for 7 days (P<0.05). Furthermore, 10% DMSO combined with 15% egg yolk with an pre-freezing time of 20 min can maintain sperm motility, viability, and fertility higher than other treatments, by 79%, 80%, and 33%, respectively. The agarose gel electrophoresis showed no DNA fragmentation in all samples, including fresh sperm. CONCLUSION: We conclude that 10% DMSO combined with 15% egg yolk and 20 min pre-freezing is the best treatment for goldfish sperm cryopreservation.
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İncele - MakaleYalnız künye2022Scopus SJRQ2Effect of increasing water temperature on the physiology and gill histology of Barramundi, Lates calcarifer (Pisces, Perciformes) fingerlings
Amir, Fachrurozi; Muchlisin, Zainal A.; Nur, Firman M.; Fadli, Nur; Siti-Azizah, Mohd N.; Wilkes, Martin; Tang, Usman M.; Hasan, Bustari; Batubara, Agung S.; Kocabas, Filiz Kutluyer
Barramundi, Lates calcarifer is one of the commercial fish in Indonesia and other Southeast Asian countries. The species inhabits the coastal waters and is vulnerable to changes in the terrestrial environment due to coastal degradation, pollution, and global warming. The increase in temperature as a consequence of global warming affects the distribution and survival of aquatic organisms including fish. Therefore, this study aims to examine the effect of increasing water temperatures on the physiological conditions and gill histology of barramundi. The fish were exposed to eight levels of water temperature, ranging from 28oC to 35oC, for 15 days. The initial temperature was 28oC, which increased gradually by 1oC per 12-hour. Blood samples were taken at the end of the experiment for hemoglobin, glucose, cortisol, erythrocytes, leucocytes, and blood abnormality analyses. Gill samples were also taken for histological analysis. The results showed that water temperature significantly affected the level of glucose, cortisol, and erythrocyte cells (P<0.05), but did not influence hemoglobin levels and survival rate (P>0.05). A rapid increase in glucose and cortisol levels was also discovered at a temperature of 31oC above. The highest erythrocyte cell count was found at 35oC, gill cell abnormalities were occurred at temperature of 31oC and above, and the blood abnormalities increased linearly with water temperature. Based on these results, it is concluded that the water temperature did not affect the survival rate but the increase in temperature causes intense physiological stress.
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