Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYalnız künye2019Scopus SJRQ3Effect of L-Tryptophan on Sperm Quality of Tigris Scraper (Capoeta umbla) (Pisces: Cyprinidae) after Cryopreservation
Kutluyer, F.; Aksu, Ö.; Kocabaş, M.
BACKGROUND: The aromatic amino acid L-tryptophan is a versatile molecule and needed for the biosynthesis of proteins. Due to metabolic functions, it has been widely used in research and clinical trials. OBJECTIVE: The present study aimed to test the usefulness of L-tryptophan as an extender of Tigris scraper (Capoeta umbla) sperm during cryopreservation. MATERIALS AND METHODS: Semen samples were diluted with a ratio of 1:3 (sperm: extender) and base extenders were supplemented with L-tryptophan (0 mM, 0.5 mM, 1 mM, 2 mM and 4 mM). Sperm motility and duration of post-thawed samples were assessed after the cryopreservation process. RESULTS: Our results revealed that motility rate and duration of sperm increased when the cryomedia was supplemented with L-tryptophan. On the other hand, an increase in the concentration of L-tryptophan in the extender caused a significant decrease (p<0.05) in the motility rate of Tigris scraper (C. umbla) sperm. The ideal results were obtained at 1 mM L-tryptophan; however, no motile sperm were shown in any samples when a concentration of 2 mM was used. CONCLUSION: The current study shows the importance of conducting further studies related to long-term storage and reproduction management.
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İncele - MakaleAçık Erişim2016Scopus SJRQ3INFLUENCE OF STOCK DENSITY ON DIGESTIVE ENZYME ACTIVITY (TRYPSIN), HEAT SHOCK PROTEIN 70 (HSP70), AND OXIDATIVE STRESS BIOMARKERS OF NARROW CLAWED CRAYFISH, ASTACUS LEPTODACTYLUS ESCHSCHOLTZ, 1823 (DECAPODA, ASTACIDAE)
Aksu, O.; Kutluyer, F.; Can, E.; Erisir, M.; Benzer, F.
Stocking density causes changes in behaviour and physiology of aquatic animals and high density can cause oxidative damage in living cells. Therefore, experiments were designed to investigate the effects of stocking density on the oxidant-antioxidant response (glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT), and malondialdehyde (MDA)), digestive enzyme activity (trypsin), and heat shock protein 70 (HSP70) of narrow clawed crayfish (Astacus leptodactylus). The experiment was conducted to assess effects by stocking 3, 6, 9 and 12 crayfish/m(2). Crayfish were stocked into 12 fiberglass tanks, three replicates per treatment and fed for 45 days. Our results indicated that activity of GSH-Px, MDA, and GSH levels increased in hepatopancreas (p<0.05) and muscle tissues (p<0.05) with increasing stock concentration while CAT activity decreased (p<0.05). Trypsin and HSP70 insignificantly increased in haemolymph. In conclusion, oxidative stress biomarkers were negatively influenced by increased quantitative changes in stocking density.
AtıfWoS6OpenAlex8İncele - MakaleYalnız künye2016Scopus SJRQ3Combined effects of physicochemical variables (pH and salinity) on sperm motility: characterization of sperm motility in European sea bass Dicentrarchus labrax
Ögretmen, F.; Inanan, B. E.; Kutluyer, F.
Gamete activation in fish is an important step in terms of artificial fertilization of oocytes, cryopreservation studies and other experimental manipulations. Salinity and pH differences in activation media affect to sperm motility and fertilizing ability. These experiments were therefore designed to investigate the combined effects of pH (range 5.0-9.0) and salinity (20, 30, 37, and 45 parts per thousand) of activation media on sperm motility of European sea bass Dicentrarchus labrax. The best results were obtained at salinity 37 parts per thousand and a pH of 9.0. Our results also demonstrated that non-progressive motility at salinity 45 parts per thousand was observed in the range of 5.0-9.0 pH. In conclusion, spermatozoa can be motile at a wide range of pH and salinity values although the percent of motile spermatozoa and motility duration are negatively affected by low pH values.
AtıfWoS13OpenAlex17İncele - MakaleAçık Erişim2016Scopus SJRQ4Comparison of number and shape of parr marks in three species of the genus Salmo and two ecotypes of cultured brown trout Salmo trutta from Turkey
Kocabas, M.; Bascinar, N.; Kutluyer, F.
This study focused on the variation in number and shape of parr marks in three fish species of the genus Salmo [S. abanticus Tortonese, (1954), S. caspius Kessler, (1877) and S. labrax Pallas, (1814)] and two ecotypes of cultured brown trout [S. trutta macrostigma (Dumeril, 1858) and S. trutta fario Linnaeus, (1758)]. The brown trouts were collected from 11 streams in Turkey by electrofishing and transferred to the laboratory in an aerated container and then stocked in the adaptation tanks. Artificially fertilized eggs were incubated and larvae were maintained in fiberglass tanks and the variations in number and shape of parr marks in all five groups were studied for one year. The data collected indicated that there were significant differences in the number and size (length and width) of parr marks among the groups (p<0.05). The highest number of parr marks (11-13) was recorded in S. trutta macrostigma. Parr marks on the skin of S. trutta. macrostigma was found to remain stable during development.
AtıfWoS2OpenAlex2İncele - MakaleYalnız künye2012Scopus SJRQ3HATCHING PERFORMANCES AND YOLK SAC ABSORPTIONS OF CASPIAN BROWN TROUT (SALMO TRUTTA CASPIUS T., 1954)
Kocabas, M.; Basçinar, N.; Sahin, S. A.; Kutluyer, F.
Salmo trutta caspius T., 1954 is a native to Caspian Sea and is an important fish species due to its aquaculture potential, economic value and high marketability. The aims of this study were to determine hatching rate, body and yolk weight at hatching, the growth rate during the yolk absorption and yolk conversion efficiency of S. I. caspius larvae. Studies were further extended to determine the relationships between length, total wet weight, dry yolk body weights of alevins and degree-days. Results revealed that the mean wet weight was 16.78 +/- 2.17 mg (n = 10) at hatching and reached 52.62 +/- 3.55 mg just before the swim-up stage. The mean dry weights of the body and yolk sac were 2.36 +/- 0.28 mg and 10.67 +/- 0.67 mg at hatching and 7.21 +/- 0.71 mg and 2.57 +/- 0.62 mg at swim-up stages, respectively. The mean body dry matter and water content of the larvae were 33.10% and 66.90% at hatching and 15.46% and 84.54% of the total biomass at swim-up stages, respectively. Our results indicated that dry yolk, total larval weights and dry matter of the larvae decreased, while body weight and water content increased with degree-days. The growth of larva, yolk sac absorption and yolk conversion efficiency were calculated as 0.18 mg/day, 0.23 mg/day and 0.60, respectively. The data collected during these studies contribute to the management practices of the stocks of this species.
AtıfWoS2İncele - MakaleYalnız künye2011Scopus SJRQ3The Reproduction Traits of Native Brown Trout (Salmo trutta macrostigma T., 1954), Turkey
Kocabas, M.; Kayim, M.; Can, E.; Kutluyer, F.; Aksu, O.
Some key aspects of the reproductive strategy of the brown trout Salmo trutta macrostigma (T., 1954) inhabiting Uzungol stream, Trabzon including spawning season, age at sexual maturity, fecundity and egg size have been studied between January 2009 to May 2010. The ages of the captured fish ranged from 2-8 years. Individuals were composed of 40.85% males and 37.80% females. The present investigation on the fecundity of S.t. macrostigma was carried out to estimate the average and range in the number of ova laid by individual female as well as to study the relationship of the fecundity with total length, total body weight. The mean value of absolute fecundity was 312.23 +/- 254.71 (SD) eggs/female. The regression between egg number and fish size (total length and weight) was statistically significant (p<0.05).
AtıfWoS7İncele - MakaleYalnız künye2011Scopus SJRQ3Stomach Contents and Food Habits of Brown Trout (Salmo trutta L., 1758) in the Munzur Stream, Turkey
Kocabas, M.; Kayim, M.; Can, E.; Ates, M.; Kizak, V.; Kutluyer, F.; Demirtas, N.; Serdar, O.
In the present study, biometric characteristics and stomach contents of Salmo trutta (L., 1758), captured from Munzur stream in Tunceli, Turkey were investigated between September 2007 and December 2009. It was calculated that the age range were between 1 and 5 years old. Captured fish were measured 19.5 +/- 5.9 cm, 113.55 +/- 100.22 g (n = 120), respectively. The condition factor of trout calculated 1.14 +/- 0.20 (n = 120). At the end of study, the rations in total organism number were Decapoda (43.59%), Trichoptera (19.21%), Haplotaxidae (18.59%), Ephemeroptera (10.36%), Odonate (2.74%), Coleoptera (2.74%), Clitellata (2.43%) and Diptera (0.3%), respectively. The results indicated that the fish feed basically on Arthropoda and Annelidae.
AtıfWoS1İncele