Muchlisin, Zainal Abidin; Tasnarizki, Dwimutia; Risky, Muhammad; Diani, Diani; Naurah, Aufa; Dewi, Cut Dara; Maulida, Siti; Razi, Nanda Muhamamd; Rahayu, Sri Riska; Handayani, Luvi Syafrida

The commercial aquaculture of seurukan fish (Osteochilus jeruk) is common in Indonesia; however, seed availability from the wild is limited. Therefore, induced breeding technology in seurukan fish using synthetic reproductive hormones is crucial. This study evaluated the efficacy of five different types of synthetic reproductive hormones and their corresponding doses on the reproductive performance of seurukan fish in captivity. The methodology consisted of two experiments; first, four types of synthetic reproductive hormones were tested: ovaprim, ovaspec, human chorionic gonadotropin (HCG), and ovalumon. Second, several doses of ovaprim hormone were stested with doses of 0.25, 0.50, 0.75, and 1.0 ml kg-1 body weight. The results showed that ovaprim, ovaspec, and HCG successfully induced ovulation and spawning in seurukan fish. The hormones significantly affected the latency period, number of eggs released, fertilization, and survival rates (P<0.05), but did not significantly affect the hatching rate and survival of larvae (P>0.05). Ovaprim produced better egg quality compared to ovaspec and HCG. In the second experiment, the results showed a significant difference in the latency period and egg size (P<0.05) of fish treated with varying doses of ovaprim hormone. The hormone doses did not significantly affect the number of eggs, hatching rate, fertilization rate, survival rate, or larval survival (P > 0.05). The dose of 0.5 ml kg-1 BW of ovaprim produced the best results. Therefore, a dose of 0.5 ml kg-1 BW was recommended as an effective dose for induced spawning and seed production of seurukan fish.

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Handayani, Luvi S.; Maulida, Siti; Rahayu, Sri R.; Razi, Nanda M.; Kocabas, Mehmet; Kocabas, Filiz K.; Eriani, Kartini; Fadli, Nur; Siti-Azizah, Moh N.; Marimuthu, Kasi

The study aims to determine the type of extender and the best dilution ratio for short preservation walking catfish Clarias batrachus sperm at a refrigeration temperature of 4°C. The study employed a completely randomized design with two factors, each consisting of 6 treatments and 3 replications. The experiments were divided into 2 stages: the first stage tested several types of extenders, namely tap water, Ringer’s solution, physiological solution, Alsever’s solution, urea solution, and glucose base solution, all at the same dilution level of 1:20 (sperm: extender, v/v). The second stage examined various levels of sperm dilution ratios, namely 1:10, 1:20, 1:30, 1:40, 1:50, and 1:60 (sperm: extender, v/v). The samples were stored at a refrigerated temperature of 4°C. Furthermore, motility and viability were monitored at 24-hour intervals for next 6 days, and on the final day of the experiment. The ANOVA test showed that the type of extender and dilution ratio significantly affected the motility and viability of the spermatozoa and percentage of fertilization (P < 0.05). Generally, the quality of sperm decreased after preservation for 144 hours at 4°C, but diluted with Ringer solution at a ratio of 1:40 (sperm: extender, v/v), still maintains good parameters. Therefore, it is recommended that Ringer’s solution at a dilution ratio of 1:40 is suitable for preserving walking catfish C. batrachus sperm at a refrigerated temperature of 4°C.

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Amir, Fachrurozi; Muchlisin, Zainal A.; Nur, Firman M.; Fadli, Nur; Siti-Azizah, Mohd N.; Wilkes, Martin; Tang, Usman M.; Hasan, Bustari; Batubara, Agung S.; Kocabas, Filiz Kutluyer

Barramundi, Lates calcarifer is one of the commercial fish in Indonesia and other Southeast Asian countries. The species inhabits the coastal waters and is vulnerable to changes in the terrestrial environment due to coastal degradation, pollution, and global warming. The increase in temperature as a consequence of global warming affects the distribution and survival of aquatic organisms including fish. Therefore, this study aims to examine the effect of increasing water temperatures on the physiological conditions and gill histology of barramundi. The fish were exposed to eight levels of water temperature, ranging from 28oC to 35oC, for 15 days. The initial temperature was 28oC, which increased gradually by 1oC per 12-hour. Blood samples were taken at the end of the experiment for hemoglobin, glucose, cortisol, erythrocytes, leucocytes, and blood abnormality analyses. Gill samples were also taken for histological analysis. The results showed that water temperature significantly affected the level of glucose, cortisol, and erythrocyte cells (P<0.05), but did not influence hemoglobin levels and survival rate (P>0.05). A rapid increase in glucose and cortisol levels was also discovered at a temperature of 31oC above. The highest erythrocyte cell count was found at 35oC, gill cell abnormalities were occurred at temperature of 31oC and above, and the blood abnormalities increased linearly with water temperature. Based on these results, it is concluded that the water temperature did not affect the survival rate but the increase in temperature causes intense physiological stress.

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