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- MakaleAçık Erişim2024Scopus SJRQ4MOLECULAR MARKERS ANALYSIS OF ENDEMIC BORNMUELLERA HAUSSKN. SPP. (BRASSICACEAE) IN TÜRKİYE; [ANALIZA MOLEKULARNIH MARKERA ENDEMIČNE VRSTE BORNMUELLERA HAUSSKN. SPP. (BRASSICACEAE) U TURSKOJ]
Okan, Kemalcan; Özkara, Zekiye; Sevİndİk, Emre; Sevİndİk, Mustafa; Paksoy, Mehmet Yavuz
Okan K., Z. Özkara, E. Sevindik, M. Sevindik, M. Y. Paksoy (2024). Molecular markers analysis of endemic Bornmuellera hausskn. spp. (Brassıcaceae) ın Türkiye. - Genetika, Vol 56, No.2, 295-304. In this study, molecular characterisation of Türkiye's endemic species Bornmuellera cappadocica (Willd.) Cullen & T.R. Dudley, Bornmuellera glabrescens (Boiss. & Balansa) Cullen & T.R.Dudley, Bornmuellera kiyakii Aytaç & Aksoy and Bornmuellera angustifolia (Hausskn. ex Bornm.) Cullen & T.R.Dudley was carried out using ten RAPD and ten ISSR primers. In RAPD-PCR analysis, 66 bands were obtained and the polymorphism rate was 96.96%. In the ISSR-PCR analysis, 119 bands were obtained and the polymorphism rate was 95.79%. In the UPGMA (Unweighted Pair Group Method with Arithmatic Mean) dendrogram based on RAPD-PCR, B. angustifolia and B. glabrescens were found to be a sister group, and B. kiyakii and B. cappadocica were a sister group. Principal Component Analysis (PCA) analysis based on RAPD-PCR were compatible with the UPGMA dendrogram. In the UPGMA dendrogram based on ISSR-PCR, B. kiyakii and B. glabrescens were found to be sister groups, and B. cappadocica was closely related to this group. PCA analysis based on ISSR-PCR were compatible with the UPGMA dendrogram. As a result, both RAPD and ISSR results a high rate of polymorphism were obtained. The results were compared with previous sequence-based studies, morphological, anatomical and palynological studies.
- MakaleYalnız künye2024Scopus SJRQ2Phylogenetic analysis of the endemic Bornmuellera Hausskn. spp. (Brassicaceae) in Türkiye based on nuclear ITS and chloroplast trnL intron, trnL-F, rbcL and trnQ-rps16 DNA sequences
Okan, Kemalcan; Sevindik, Emre; Paksoy, Mehmet Yavuz
In this study, phylogenetic analysis of Turkish endemic Bornmuellera (Brassicaceae) species was conducted based on nuclear ribosomal DNA (ITS) and chloroplast DNA (trnL intron, trnL-F, rbcL and trnQ-rps16 DNA) sequences. In addition, the relationships between the sequences of some Brassicaceae family species retrieved from NCBI, and Bornmuellera species were documented. All of the plant specimens were collected from different regions of Türkiye, and brought to the laboratory. Total genomic DNA was extracted using the GeneMark kit. After polymerase chain reaction (PCR) analyses, DNA sequences obtained were then edited using Sequencher 5.4, Bioedit 7.2.3 and FinchTV 1.4.0, and analyzed using MEGA 6.0 software. Maximum likelihood (ML) and bootstrap trees were constructed in order to identify the relationships among Bornmuellera species. In the study, the highest nucleotide diversity was detected by rps16-trnQ (0.039146) sequence analysis, while the lowest was detected by rbcL (0.004186) sequence analysis. In addition, according to the phylogenetic analysis results obtained with other Brassicaceae species, it is revealed that the Turkish endemic Bornmuellera genus is monophyletic.
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