Sürdürülebilir Kalkınma Amaçları

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• MakaleYalnız künye2022Scopus SJRQ1/r/munzur/2787
  In vivo and in vitro evolution of the effects of cypermethrin on turbot (Scophthalmus maximus, Linnaeus, 1758) spermatozoa

  Beken, Atife Tuba; Saka, Şahin; Aydın, İlhan; Fırat, Kürşat; Suzer, Cüneyt; Benzer, Fulya; Erişir, Mine; Özden, Osman; Hekimoğlu, Müge Aliye; Engin, Serhat

  Synthetic pyrethroid pesticide is commonly used in agricultural activities in the Black Sea region during reproduction period of turbot. In this sense, in vivo and in vitro studies have shown that cypermethrin (CYP) could be one of the environmental factors affecting decreasing turbot stocks. In this study, effects of in vivo and in vitro administration of CYP, a synthetic pyrethroid, on sperm kinematics motility (MOT), progressive motility (PM), curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN), straight line velocity (STR), amplitude of lateral head (ALH), beat cross frequency (BCF), oxidative stress biomarkers malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and lipid peroxidation (LPO) and also histopathological alterations in gonads were investigated in spermatazoa of turbot (Schopthalmus maximus). Broodstock was supplied from culture origin and used in spawning season, additionally, two (0, 0.187 and 0.218 ppb) and three (0, 1.025, 2.05 and 4.1 ppb) different CYP concentrations were performed for in vivo and in vitro studies, respectively. In vivo and in vitro studies, significant reductions were found in sperm MOT, PM, VCL, VSL, VAP, LIN, and ALH properties depend on the increase in CYP concentrations (p < 0.05). Besides, activities of GSH, GPx, SOD, and CAT increased. In terms of histological alterations, no difference was observed among groups (0, 0.187 and 0.218 ppb) in the maturity stage of the germ cells. According to obtained results, sperm kinematics was affected significantly with increased the dose levels of CYP (p < 0.05).

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  AtıfScopus6·OpenAlex8

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• MakaleYalnız künye2010Scopus SJRQ3/r/munzur/5652
  Sparidae familyasından çipura (Sparus aurata), fangri (Pagrus pagrus) ve sinarit (Dentex dentex) yumurtalarının farklı dezenfektan maddelerle yüzey dezenfeksiyonu

  Fırat, Muammer Kürşat; Saka, Şahin; Can, Erkan

  The aim of this study is to determine suitable conditions for egg disinfection of gilthead sea bream (Sparus aurata), red porgy (Pagrus pagrus), and common dentex (Dentex dentex) with glutaraldehyde, hydrogen peroxide and iodine. The eggs were disinfected with 200 ppm glutaraldehyde (T= 2, 4, 8, 16 min), 300 ppm hydrogen peroxide and iodine (T= 5, 10, 15, 20 min) at four different contact times. The hatching rate was determined in triplicates for each treatment. Also, bacteria colonies were counted on tryptic soy agar (TSA) and thiosulphate citrate bile salt sucrose agar (TCBS). At the end of the experiment, bacterial load and hatching rate were assessed together. For glutaraldehyde treatment, optimal hatching rates and bacterial colonies were estimated as CT (Disinfectant concentration in mg.l-1* exposure time in minute) 800 for sea bream eggs, CT 1600 for red porgy eggs and CT 800-1600 for common dentex eggs. Also, for hydrogen peroxide treatments, they were determined as CT 3000 for sea bream and red porgy and CT 4500 for common dentex. Finally, in iodine treatments, these parameters were measured as CT 4500-6000 for all species&amp;#8217; eggs. However, in our study, it is clear that the glutaraldehyde showed the optimum disinfection effect against the microorganisms because the bacteria could be eliminated completely.

  AtıfTR Dizin4

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