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- MakaleAçık Erişim2024Scopus SJRQ4MOLECULAR MARKERS ANALYSIS OF ENDEMIC BORNMUELLERA HAUSSKN. SPP. (BRASSICACEAE) IN TÜRKİYE; [ANALIZA MOLEKULARNIH MARKERA ENDEMIČNE VRSTE BORNMUELLERA HAUSSKN. SPP. (BRASSICACEAE) U TURSKOJ]
Okan, Kemalcan; Özkara, Zekiye; Sevİndİk, Emre; Sevİndİk, Mustafa; Paksoy, Mehmet Yavuz
Okan K., Z. Özkara, E. Sevindik, M. Sevindik, M. Y. Paksoy (2024). Molecular markers analysis of endemic Bornmuellera hausskn. spp. (Brassıcaceae) ın Türkiye. - Genetika, Vol 56, No.2, 295-304. In this study, molecular characterisation of Türkiye's endemic species Bornmuellera cappadocica (Willd.) Cullen & T.R. Dudley, Bornmuellera glabrescens (Boiss. & Balansa) Cullen & T.R.Dudley, Bornmuellera kiyakii Aytaç & Aksoy and Bornmuellera angustifolia (Hausskn. ex Bornm.) Cullen & T.R.Dudley was carried out using ten RAPD and ten ISSR primers. In RAPD-PCR analysis, 66 bands were obtained and the polymorphism rate was 96.96%. In the ISSR-PCR analysis, 119 bands were obtained and the polymorphism rate was 95.79%. In the UPGMA (Unweighted Pair Group Method with Arithmatic Mean) dendrogram based on RAPD-PCR, B. angustifolia and B. glabrescens were found to be a sister group, and B. kiyakii and B. cappadocica were a sister group. Principal Component Analysis (PCA) analysis based on RAPD-PCR were compatible with the UPGMA dendrogram. In the UPGMA dendrogram based on ISSR-PCR, B. kiyakii and B. glabrescens were found to be sister groups, and B. cappadocica was closely related to this group. PCA analysis based on ISSR-PCR were compatible with the UPGMA dendrogram. As a result, both RAPD and ISSR results a high rate of polymorphism were obtained. The results were compared with previous sequence-based studies, morphological, anatomical and palynological studies.
- MakaleAçık Erişim2022Scopus SJRQ2DNA barcoding and phylogenetic analysis of endemic Astragalus nezaketiae and Vicia alpestris subsp. hypoleuca (Fabaceae):Evidence from nrDNA ITS and cpDNA matK and rbcL sequences
Paksoy, Mehmet Y.; Sevİndİk, Emre; Başköse, İsa
In this study, we performed DNA barcoding and phylogenetic analysis using one nuclear (ITS) and two chloroplast DNA regions (matK and rbcL) of endemic Astragalus nezaketiae A. Duran & Aytaçand Vicia alpestris Stev. subsp. hypoleuca (Boiss.) Davis taxa in Turkey. PCR reactions were performed using universal primers. Sequences of the PCR products were edited using BioEdit and FinchTV software and contigs were obtained. All contigs were Blasted at NCBI and similarities were analysed. Using the MEGA 6.0 program, maximum likelihood trees were constructed including some sequences retrieved from NCBI. For Astragalus nezaketiae; in the ITS analysis, Astragalus nezaketiae appeared separately from other species, and for matK, Astragalus nezaketiae appeared together with Astragalus cicer L. However, rbcL tree was polytomic. For Vicia alpestris subsp. hypoleuca; in ITS, rbcL and matK results Vicia alpestris subsp. hypoleuca were found together with Vicia cracca L., Vicia benghalensis L. and Vicia villosa Roth species. Analysis of the combined data revealed similar results with all barcode regions for Vicia alpestris subsp. hypoleuca while different phylogenetic results were obtained for Astragalus nezaketiae.
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