Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleÖn baskısı açık2016Scopus SJRQ2Cytokine Responses in Gills of Capoeta umbla as Biomarkers of Environmental Pollution
Danabas, Durali; Yildirim, Nuran Cikcikoglu; Yildirim, Numan; Onal, Ayten Oztufekci; Uslu, Gulsad; Unlu, Erhan; Danabas, Seval; Ergin, Cemil; Tayhan, Nilgun
Immunological biomarkers reflect the effects of exposure to environmental contaminants. In this study, the suitability and sensitivity of cytokine responses, interleukin 1 beta (IL-1 beta), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha) in gill tissues of Capoeta umbla (Heckel, 1843), collected from different regions, as early warning indices of environmental pollution and ecosystem health was evaluated. Fish and water samples were taken from ten stations in March and September 2011 and 2012. Tumor necrosis factor-alpha, IL-1 beta and IL-6 levels were determined in samples of the gill tissues by using an ELISA kit. Significant variations of TNF-alpha, IL-1 beta and IL-6 levels observed between stations and seasons. The results of this study show that seasonal variations of cytokine responses in gills of Capoeta umbla are sensitive to the contaminants present in Uzuncayir Dam Lake (Tunceli, Turkey) water and are valuable biomarkers for environmental pollution and ecosystem health.
AtıfWoS5OpenAlex4
İncele - MakaleAçık Erişim2015Scopus SJRQ4Optimization of lipase production on agro-industrial residue medium by Pseudomonas fluorescens (NRLL B-2641) using response surface methodology
Tanyol, Mehtap; Uslu, Gulsad; Yönten, Vahap
The aim of our research was to explore the most cost-efficient and optimal medium composition for the production of lipase from Pseudomonas fluorescens (NRLL B-2641) culture grown on sunflower oil cake (SuOC) by applying response surface methodology (RSM). The oil cake was used instead of carbon sources. Peptone, ammonium sulphate and the carbon source (SuOC) were the most important factors as it is obligatory for microbial growth. Subsequently, the optimum values for the carbon source, peptone and ammonium sulphate were found to be 11.10% (w/v), 1.18% (w/v) and 0.83% (w/v), respectively. Experiments carried out under optimum conditions revealed a maximum lipase activity of 10.8 U mL(-1), which was achieved after 48 h of fermentation. The obtained results were finally verified with batch experiments carried out under the optimum conditions evaluated and it was demonstrated that the SuOC from agro-industrial residue as substrates can be used as an inexpensive base (carbon source) for the production of lipase by P. fluorescens (NRLL B-2641).
AtıfWoS19İncele - MakaleAçık Erişim2015Scopus SJRQ3Changes in antioxidant defense system in gills of Capoeta umbla caught from Uzuncayir Dam Lake, Turkey
Danabas, Durali; Yildirim, Nuran Cikcikoglu; Yildirim, Numan; Onal, Ayten Oztufekci; Uslu, Gulsad; Unlu, Erhan; Danabas, Seval; Ergin, Cemil; Tayhan, Nilgun
The aim of this work was to determine the seasonal changes in the activities of selected biomarkers in Capoeta umbla (Heckel, 1843) caught from Uzuncayir Dam Lake (Tunceli, Turkey) and to evaluate the effects of environmental factors on these activities. Fish were sampled on seasonal basis, and superoxide dismutase, catalase, glutathione peroxidase activities and levels of glutathione and malondialdehyde in gills were determined. Significant variations of oxidative stress biomarkers were observed between seasons and sites. The results of this study show that seasonal variations of oxidative stress responses and lipid peroxidation in gills of C. umbla are sensitive to the contaminants present in water of Uzuncayir Dam Lake and selected parameters are in valuable biomarkers for monitoring of water systems, since they give an early warning signal of effects of xenobiotics on aquatic organisms at molecular levels which help to prevent their effects at organismal level. (C) 2015 Elsevier Ltd. All rights reserved.
AtıfWoS14OpenAlex10İncele - MakaleYalnız künye2014Scopus SJRQ4Production of Lipase by P. fluorescens (NRLL B-2641) under Submerged Fermentation Conditions
Tanyol, Mehtap; Uslu, Gulsad
Lipases are industrially important enzymes produced by a great variety of microorganisms. In this study, the production of extracellular lipase by Pseudomonas fluorescens (NRLL B-2641) using submerged fermentation was studied: The effects of. initial pH, carbon source, nitrogen source and metal ion addition on lipase activity were investigated using batch experiments. The highest enzyme activity and biomass production obtained were 2.50 U mL(-1) and 2.95 g cell dry wt. L-1, respectively, using a fermentation medium with 1% corn oil as the carbon source, 0.4% peptone as the nitrogen source and an initial pH of 6.0. The addition of calcium increased the enzyme activity to 3.30 U mL(-1). Thus, initial lipase activity, which was 0.92 U mL(-1), increased 356%. Partial characterization of crude lipase was performed to determine the optimum pH and temperature. The crude lipase displayed high activities at pH 6.0-8.5 and 35-65 degrees C, with maximum activity at pH 8.0 and 50 degrees C.
- MakaleAçık Erişim2013Scopus SJRQ2Characterization and pollution potential assessment of Tunceli, Turkey municipal solid waste open dumping site leachates
Demirbilek, Deniz; Önal, Ayten Oztufekci; Demir, Veysel; Uslu, Gulsad; Arslanoglu-Isik, Hilal
Environmental monitoring of leachate quality from an open municipal solid waste dumping site in Tunceli, Turkey was studied in this research. The most commonly examined pollution parameters were determined on a seasonal basis. The annual average 5-day biological oxygen demand (BOD5) and chemical oxygen demand (COD) values of station points were measured as 70 and 425 mg/L, respectively, and also the average BOD5/COD ratio (a measure of biodegradability) was calculated as 0.20. The low ratio of biodegradability and slightly alkaline pH values in the leachate samples indicated that the site was characterized by methanogenic conditions. The mean ammonium-nitrogen (NH4 (+)-N) and corresponding phosphate (orthophosphate) values were assayed as 70 and 11 mg/L, respectively. The average solids content in the leachates was measured as 4,681 mg/L (total solids) and 144 mg/L (suspended solids). Very low concentrations of iron, manganese, copper, and zinc in the leachate samples were found and the concentration of cadmium was measured below detection limits. Excessive amount of nutrients and high organic and inorganic pollutant content in the leachates pose serious pollution potential to the environment. Since no drainage system or bio treatment exists in this open dumping site, high permeability of natural soil at the site and in the surrounding area and very fractured and crackled rocks under natural soil are indicators of high groundwater pollution potential in this site.
AtıfWoS23OpenAlex21İncele - BildiriAçık Erişim2012Scopus SJRQ2The effect of initial glucose concentration and of inducer addition on the production lipase by Pseudomonas fluorescens
Tanyol, Mehtap; Uslu, Gulsad
- MakaleYalnız künye2011Scopus SJRQ3BIOACCUMULATION OF COPPER (II) AND CADMIUM (II) FROM AQUEOUS SOLUTION BY PSEUDOMONAS PUTIDANRRL B-252
Uslu, Gulsad; Gültekin, Gulden; Tanyol, Mehtap
The bioaccumulation of copper (II) and cadmium (II) ions by viable P. putida was studied as a function of initial pH, temperature, initial glucose and initial metal ion concentration. Optimum pH value for maximum metal ion accumulation was determined to be 3.0 +/- 0.5 and 4.5 +/- 0.5 at 25 and 30 degrees C for each metal ion, respectively. At a constant glucose concentration, although the increase in initial copper (II) and cadmium (II) ion concentration up to approximately 25 mg L-1 increased bioaccumulation capacity of P. putida for both, all metal ion concentrations tested inhibited the growth of P. putida. High microbial growth and copper (II) and cadmium (II) accumulation yields were found at low initial metal ion concentrations. The cell and bioaccumulated metal ion concentrations and uptake yields in-creased with increasing glucose concentration up to 10 g L-1 at a constant metal ion level for each of the metal ions. Although both metal ions caused on inhibition effect on the growth of the microorganism, P. putida was capable of removing copper (II) and cadmium (II) with maximum specific uptake of 58.42 mg g-(1) and 34.65 mg g(-1) at 25 mg L-1 initial copper(II) and cadmium(II) level, respectively. The results also indicated that, P. putida was more efficient in accumulating larger amounts of cadmium (II) than copper (II) ions.
AtıfWoS2İncele