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- MakaleAçık Erişim2022Anatomical, Micromorphological, Karyological and Biochemical study of Scutellaria orientalis subsp. virens and Scutellaria salviifolia
Acar, Mikail; Tasar, Neslihan; Akbulut, Guelcin Beker
In this study, the anatomical and micromorphological structure, karyological characteristics and biochemical content of Scutellaria orientalis subsp. virens and endemic Scutellaria salviifolia, whose distributions areas overlap, were compared. Some anatomical and micromorphological differences were observed on the taxa; scleranchymatic pericycle layer on the stem, stomata density, distribution of trichomes, as well as the main vascular bundle and general shape of the petiole. The chromosome numbers of both taxa were determined as 2n = 22. However, there was a difference between chromosome length range and total chromosome length. The chromosome numbers and chromosome morphologies of these species have been defined for the first time in this paper. Differences in biochemical content were observed between species. Chlorophyll a (Chl a), total chlorophyll (Total Chl), total carbohydrate and malondialdehyde (MDA) contents were determined higher in leaf and stem samples of S. orientalis subsp. virens than S. salviifolia. There was no significant difference between the two taxa in terms of chlorophyll b (Chl b) content. Carotenoid (Car) content was detected higher in leaves samples of S. orientalis subsp. virens, but no significant difference was found between stems samples. Also, the effect of taxa on biochemical contents in relation to the habitat they live in is given in this study.
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İncele - MakaleAçık Erişim2019Comparative Micromorphological and Anatomical Investigations on the Subsection Distantes R. Bhattacharjee (Stachys L./Lamiaceae)
Açar, Mikail; Satil, Fatih
In this study; comparative anatomical and micromorphological studies were performed on the subsection Distantes of the Stachys L. genus (Lamiaceae). Also, it was determined distinctive anatomical characters for the taxa of subsection in the study. In anatomical studies, free-hand cross-sections and the paraffin embedding method was applied with the microtome. Micromorphological studies were performed in LM (Light Microscope) and SEM (Scanning Electron Microscope). In anatomical studies, besides of similarities in stem and leaf structures there were also some differences in taxa. In micromorphological studies, the trichome and nutlet characteristics were found to be useful characters on taxa and at species and subspecies level. As a result of the study, the presence of sclerenchyma like a bundle in the stem structure and the presence and types of glandular trichomes and ornamentation on the surface of nutlet, the presence of the wing and ventral structure in the nutlet were important characters that can be used to distinguish taxa. According to the numerical findings, trichome and nutlet micromorphological characters were more important than anatomical characters.
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