Sürdürülebilir Kalkınma Amaçları

• 1

• MakaleAçık Erişim2025Scopus SJRQ1/r/munzur/1011
  Exploring the anticancer potential of Dianthus orientalis in pancreatic cancer: A molecular and cellular study

  Yildirim, Mehmet Aykut; Sevinc, Burak; Paydas, Samet; Karaselek, Mehmet Ali; Duran, Tugce; Kuccukturk, Serkan; Vatansev, Hakan; Cetiz, Mehmet Veysi; Caprioli, Giovanni; Acquaticci, Laura

  Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal malignancies, characterized by limited therapeutic options and poor prognosis. This study investigated the anticancer effects of Dianthus orientalis extracts on pancreatic cancer cell lines (Panc-1 and BxPC-3) with a focus on apoptosis, invasion, and metastasis-related pathways. Chemical analysis using HPLC-MS/MS identified bioactive compounds (vanillic acid, syringic acid, p-coumaric acid, quercetin, hyperoxide) known for their anticancer properties. The Inhibitory Concentration 50 (IC50) value, which is half maximal inhibitory concentration of cancer cells, was determined to be 250 μg/mL at 48 h, and significantly induced apoptosis by decreasing anti-apoptotic gene expressions (Bcl-XL and Bcl-2) and increasing pro-apoptotic gene expressions (Casp3 and Bax) particularly in the Panc-1 cell line. Flow cytometric analysis revealed approximately 55% apoptosis in both cell lines. Molecular docking and dynamics analyses demonstrated strong binding affinities of quercetin and hyperoxide with important cancer-related proteins, including CHEK2 and PALB2. Gene expression analysis demonstrated the upregulation of pro-apoptotic markers like Bax and Casp3, and selective modulation of metastasis-related genes such as SNAI1, PALB2, and CHEK2. The findings highlight the potential of D. orientalis to modulate DNA repair mechanisms and epithelial-mesenchymal transition (EMT) pathways. Furthermore, network pharmacology and in silico tools corroborated its interactions with critical signaling pathways in cancer progression, including PI3K-Akt and MAPK. The study provides the first comprehensive evidence of D. orientalis's antiproliferative through apoptosis induction on pancreatic cancer cell lines, laying the groundwork for future therapeutic applications.

  AtıfScopus19

  İncele
• MakaleAçık Erişim2024Scopus SJRQ1/r/munzur/2021
  A broad-spectrum biological activities of Heracleum humile extracts: A first report of the antiviral, anti-cancer and chemical properties

  Ocal, Mustafa; Duran Yelken, Selda; Altunoglu, Yasemin Celik; Baloglu, Mehmet Cengiz; Soomro, Sanam Iram; Zengin, Gokhan; Angeloni, Simone; Mustafa, Ahmed M.; Caprioli, Giovanni; Paksoy, Mehmet Yavuz

  The current work focused on researching the bioactive potential of ethyl acetate (EA), methanol (MeOH), and water extract of H. humile plant, specifically ethyl acetate, methanol extracts were acquired with maceration method, and water extract was obtained by the infusion method. The highest total phenolic ingredient was determined in the water extract, while the highest total flavonoid ingredient was found in the MeOH extract. The water extract displayed the most potent antioxidant activity in the assays used. The ethyl acetate extract showed the highest enzyme inhibition activity in Butyrylcholinesterase inhibition (BChE) and amylase assay. Antibacterial efficiency was analyzed using microdilution analysis. The minimum inhibitory concentration (MIC) amounts ranged from 125 to 500 μg/mL except for water extract. The antiviral potential of the extracts was assessed against Bovine herpes virus 1 (BoHV-1) (Cooper strain). Methanol extract showed more potent antiviral effect. Selectivity indices were calculated for ethyl acetate and methanol extract as 3.32 and 13.39, respectively. All extracts displayed Deoxyribonucleic Acid (DNA) protection activity at approximately 25%–35%. For cell viability evaluation, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) test was performed against triple-negative human breast adenocarcinoma cells (MDA-MB-231) and Michigan Cancer Foundation-7 (MCF-7) cells. The most effective results were found in ethyl acetate extract at 48h. The 50% inhibitor concentrations (IC50) were determined for MDA-MB-231 and MCF-7 as 116.7 μg/mL and 247.9 μg/mL, respectively. Based on the bioactive compound content results, extracts of H. humile include valuable compounds that possess significant biological properties. In conclusion, these extracts could offer promising novel plant-derived therapeutics.

  • 
  İncele