Sürdürülebilir Kalkınma Amaçları

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• MakaleYayıncıda açık erişim2025Scopus SJRQ2/r/munzur/2267
  Determination of Toxic Effects of Copper in Navicula cryptocephala var. veneta by Biomarkers and Bioaccumulation Quantification

  Aydın, Ayşe Nur; Serdar, Osman; Ölçülü, Abdüllatif; Çimen, Işıl Canan Çiçek; Ak, Tuba Parlak; Derman, Taner; Sevim, Ayşegül; Yıldırım, Nuran Cikcikoğlu

  As a result of the increased use of metals such as copper (Cu), copper pollution in the environment is also increasing. In order to investigate the effect of copper mixed into the water environment and on aquatic biota, the microalga Navicula cryptocephala var. veneta was chosen as a model organism. N. cryptocephala var. veneta it was aimed to determine the accumulation and elimination amounts and some biomarker responses. Metal accumulation amounts in the organisms used in the study. The amount of metal accumulated in relation to the wet weight mass of the samples was determined using Atomic Absorption Spectophotometry (AAS) mass spectrometry. In the bioassays conducted within the scope of the study, lipid peroxidation (TBARS) and reduced glutathione (GSH) levels, superoxide dismutase (SOD) enzyme activity, glutathione peroxidase (GSH-Px) enzyme activity and catalase (CAT) enzyme activity were determined with an ELISA microplate reader at 24, 48, 72, 96 and 120 hours. According to the study data, it was observed that Cu metal accumulated in Navicula cryptocephala var. veneta and caused oxidative stress in the organism, affecting the organism by causing changes in TBARS, GSH levels and antioxidant enzymes SOD, CAT and GPx activities. Additionally, it was observed that different elimination levels occurred in the application groups.

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  AtıfScopus1·OpenAlex1

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• MakaleAçık Erişim2025Scopus SJRQ2/r/munzur/1451
  Determination of the Effect of Nanoparticle Copper on Navicula cryptocephala var. veneta by Biomarkers and Bioaccumulation Amounts

  Serdar, Osman; Aydın, Ayşe Nur; Ölçülü, Abdüllatif; Çiçek Çimen, Işıl Canan; Parlak Ak, Tuba; Derman, Taner; Pala, Ayşegül; Yıldırım, Nuran Cikcikoğlu

  Pollutants such as copper nanoparticles (CuNP), which form residues in the environment, especially in water environments, cause serious damage to the environment and the organisms living in the environment. In this study, Navicula cryptocephala var. veneta was selected as a model organism. The EC50 values of CuNP on Navicula cryptocephala var. veneta were determined as 4.94 mg\L and 3 different application concentrations were determined considering this ratio. The experiments were carried out in 3 replicates and samples were taken at 24 and 96 hours. At the end of 120 hours, samples were taken to determine the elimination values. CuNP accumulation amounts in the samples were measured by electrothermal atomic absorption spectrophotometer (ETAAS). For biomarker analysis, thiobarbituric acid (TBARS) and glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were performed using CAYMAN ELISA kit. According to the study data, as CuNP concentration and application time increased, the amount of accumulation in Navicula cryptocephala also increased. Increasing-decreasing changes were observed in elimination. CuNP caused oxidative stress in N. cryptocephala and caused changes in TBARS and GSH levels. In addition, it caused increases in SOD activities and statistically significant decreases (P<0.05) in CAT and GPx activities.

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  AtıfScopus2·WoS1

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• MakaleAçık Erişim2024Scopus SJRQ3/r/munzur/2108
  Evaluation of the Effect of Temperature on the Toxicity of Lambda-Cyhalothrin in Dreissena Polymorpha Using some Biochemical Biomarkers; [Vrednotenje vpliva temperature na toksičnost Lambda-cihalotrina v modelnem organizmu Dreissena Polymorpha z uporabo biokemijskih označevalcev]

  Yildirim, Nuran Cikcikoglu; Serdar, Osman; Ketenalp, Zozan

  Due to increasing climate change, it has become important to determine whether the dose-response relationship of organisms to some substances is affected by temperature. For this reason, in this study, it was aimed to reveal the effect of the temperature variable on the toxic response using the Dreissena polymorpha model organism and some of its biomarkers. For this purpose, acetylcholinesterase (AChE), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) levels in Dreissena polymorpha exposed to subletal concentrations of λ-cyhalothrin at different temperatures were measured using commercial ELISA kits. According to the results obtained, there was a statistically significant increase in MDA levels in the groups exposed to λ-cyhalothrin, while a decrease in GSH levels was found. AChE levels were inhibited especially in the groups exposed high concentrartion of λcyhalothrin. It was also found that the inhibition levels increased depending on the application times. While SOD enzyme activity decreased, CAT enzyme activity increased depending on the exposure concentration. It has been observed that different temperature have different effects on the toxicity of λ-cyhalothrin. It was observed that λ-cyhalothrin caused oxidative stress and neurotoxicity, and the toxicity of λ-cyhalothrin changed depending on the temperature.

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• MakaleAçık Erişim2024Scopus SJRQ3/r/munzur/1998
  Toxicity assessment of advanced biological wastewater treatment plant effluent by integrated biomarker response in zebra mussels (Dreissenapolymorpha)

  Serdar, Osman; Ketenalp, Zozan; Yildirim, Nuran Cikcikoglu

  In this study, zebra mussels (Dreissena polymorpha) were exposed to advanced biological wastewater treatment plant effluent (ABWTPE) for 96 h. At the end of the 96th hour, antioxidant parameters such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST), malondialdehyde (MDA) and glutathione (GSH) were examined. The objective of the study was to identify biomarkers that are useful for assessing the potential toxic effects of ABWTPE in freshwater environments. We observed an increase in GPX, SOD activity and MDA levels, and a decrease in CAT, GST activity and GSH levels. The results obtained in our study showed that the measured biochemical parameters (GSH, MDA, SOD, CAT, GPX and GST) are useful biomarkers in determining the possible toxicity of ABWTPE in aquatic environments.

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  AtıfScopus3·WoS3·OpenAlex3

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• MakaleYalnız künye2024Scopus SJRQ3/r/munzur/1727
  The RSM-CCD Optimization of Fenton Process for Industrial Wastewater Treatment and Determination of Treatment Efficiency Using Some Biochemical Responses in Dreissena polymorpha

  Tanyol, Mehtap; Serdar, Osman; Ketenalp, Zozan; Yildirim, Nuran Cikcikoglu; Erguven, Gokhan Onder; Yildirim, Numan

  The study investigated antioxidant biomarkers in Dreissena polymorpha to evaluate the efficiency of Fenton process (FP) in Organized Industrial Zone Wastewater (OIZW) treatment. Antioxidant enzymatic activities, including superoxide dismutase (SOD) and catalase (CAT), glutathione peroxidase (GPX), non-enzymatic antioxidants (GSH) and the indicator of lipid peroxidation (TBARS) were measured in D. polymorpha in response to treated OIZW by FP. The performance of the FP has been optimized with response surface methodology (RSM). The optimum conditions (reaction time, and H2O2 and Fe2+ concentration) for OIZW by the FP process were determined. The results showed that the highest COD removal efficiency (83.94 %) was obtained when as Fe2+ concentration=402.35 mg/L, H2O2 concentration=4100.30 mg/L, and reaction time=10.10 min (desirability=1.000). SOD and CAT enzyme was decreased in the 1/25 diluted OIZW group compared to control group. CAT and SOD enzyme activities were increased after treatment compared to control groups. No statistically significant changes were observed in GSH levels and GPx activities. TBARS levels were increased 1/25 diluted OIZW exposure groups compared to the control group. TBARS levels were decreased after treatment. The results revealed that antioxidant parameters are useful biomarkers for determining the treatment efficiency of the FP.

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  AtıfScopus2·WoS1·OpenAlex2

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• MakaleAçık Erişim2024Scopus SJRQ3/r/munzur/1576
  sThe Bioremediation of Industrial Effluents by Phanerochaete Chrysosporium: The COD, TOC Removal Efficiency and Biochemical Assessment for Dreissena Polymorpha

  Serdar, Osman; Yıldırım, Numan; Onder Erguven, Gokhan; Ketenalp, Zozan; Cikcikoglu Yildirim, Nuran; Durmus, Barbaros

  Bioremediation is one of the cheapest and easy method for biological treatment for most kind of industial wastewater. Bioremediation potential of the Phanerochaete chrysosporium for industrial wastewater from Industrial Zone Wastewater plant in Mardin, Turkey, was evaluated. The chemical oxygen demand (COD) and total organic carbon (TOC) reduction efficiencies of P. chrysoporium of on wastewater studied using biomarkers such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), Thiobarbituric Acid (TBARS) and reduced glutathione (GSH) levels in Dreissena polymorpha. The level of TBARS, a marker of lipid peroxidation was found to be high because of wastewater exposure after treatment. TBARS levels decreased (from 20.2 to 31.6 and from 20.8 to 37.5). GSH levels (from 52.3 to 15.9), CAT levels (from, 119.7 to 15.3 and from 91.1 to 13.4) SOD levels (from 3.8 to 0.9 and from 3.4 to 1.2) and GPX activity (from 177.2 to 104.4 and 174.2 to 100.5) decreased in the wastewater exposure groups during 24 and 96th hour compared to the control groups. GSH levels, CAT, SOD and GPX activity increased after treatment by P. chrysosporium. Results indicate that industrial wastewater caused oxidative stress in Dreissena polymorpha. The findings revealed that the highest removal efficiency for COD and TOC, at 86.3 % and 80.3 % respectively, occurred in the 1/20 diluted medium. P. chrysosporium has proven to be effective in the bioremediation of wastewater from the Industrial Zone Changes in biochemical parameters before and after bioremediation showed that antioxidant parameters such as CAT, SOD, GPx activities and GSH and TBARS levels can be used as biomarker to evaluate bioremediation efficiency.

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  AtıfScopus4·WoS2·OpenAlex4

  İncele
• MakaleYalnız künye2023Scopus SJRQ2/r/munzur/2794
  The potential ameliorative impacts of rare earth element cerium in dimethoate induced toxicity in freshwater mussel Dreissena polymorpha

  Serdar, Osman; Ketenalp, Zozan; Cikcikoglu Yildirim, Nuran

  The purpose of this study was to measure the antioxidant parameters to examine the potential ameliorative impacts of the rare earth element (REE) cerium (Ce) in dimethoate (DM) induced toxicity in Dreissena polymorpha. Lethal concentration 50 (LC50) of DM for 96 h was measured. The antioxidant effect of Ce was investigated by exposure of 1/2 of the LC50 value of DM together with 4, 20 and, 100 mg/L of Ce to D. polymorpha. The responses of the biomarkers were determined by measuring the superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH) and malondialdehyde (MDA) levels. SOD, CAT, GPX and GSH levels were reduced (p < 0.05), while the MDA levels increased significantly (p < 0.05) in mussels exposed to DM. When DM was exposured together with Ce, it was observed that the MDA levels decreased, SOD, CAT, GPX and GSH levels increased during 24 and 96 h. The results of our study showed that Ce exerted a strong antioxidant effect on toxicity induced by DM. It has been shown that Ce may have a compensatory effect with the positive change in the antioxidant response of D. polymorpha to DM. This study is the first report about Ce and insecticide effects in bivalves.

  AtıfScopus1·WoS1·OpenAlex1

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• MakaleYalnız künye2023Scopus SJRQ4/r/munzur/1967
  Individual and Combined Toxicity of Polycyclic Aromatic Hydrocarbons Phenanthrene and Fluoranthene in Freshwater Amphipod Gammarus pulex (L., 1758) (Amphipoda: Gammaridae)

  Yildirim, Nuran Cikcikoglu; Serdar, Osman; Derman, Taner

  The combined toxicity of polycyclic aromatic hydrocarbons on freshwater amphipod Gammarus pulex was investigated. The 14-day LC50 values of fluoranthene, phenanthrene and their combination for G. pulex were determined as 5.88±0.23, 8.31±0.57 and 7.00±0.33 mg/L, respectively. To evaluate the single and combined toxicity, sublethal concentrations of fluoranthene, phenanthrene and their combination were exposed to G. pulex for 14 days. Acetylcholinesterase, glutathione-s-transferase and cytochrome P450 1A1 enzymes were measured as biomarkers. Acetylcholinesterase activities showed significant inhibition at the end of 7 and 14 days. No statistically significant change was observed in cytochrome P450 1A1 levels at the end of the 7th day, but a significant increase was detected at the end of the 14th day. A significant inhibition was observed in glutathione-s-transferase activities at the end of the 7th and 14th days. Statistically significant changes were observed in all parameters when the application times are compared. It was determined that G. pulex exposed to different concentrations of phenanthrene and fluoranthene individually and in combination caused significant changes in activites of the all measured enzymes. It was concluded that the cytochrome P450 1A1, glutathione-s-transferase and acetylcholinesterase enzymes in G. pulex are useful biochemical markers for the determination of polycyclic aromatic hydrocarbon toxicity.

  AtıfScopus3

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• MakaleAçık Erişim2023Scopus SJRQ4/r/munzur/1661
  Single and combined effects of dimethoate and malathion on oxidative stress biomarkers in the non-target freshwater mussel Dreissena polymorpha

  Serdar, Osman; Yildirim, Numan; Yildirim, Nuran Cikcikoglu

  This study evaluates the single and combined effects of dimethoate (DI) and malathion (MA) on oxidative stress biomarkers in the freshwater mussel Dreissena polymorpha, estimating the potential harm of these pesticides on aquatic ecosystems. Superoxide dismutase (SOD) and catalase (CAT) activities, and glutathione (GSH) and lipid peroxidation (TBARS) levels were determined in D. polymorpha exposed to sublethal concentrations of DI, MA and a combination of the two during 24 and 96 h periods. The results showed that the GSH levels were decreased but the TBARS levels were increased in all the exposure groups after 24 and 96 h compared to the control. It was observed that SOD activity decreased but CAT activity increased in all the DI exposure groups after 24 h compared to the control. At the end of 96 hours, it was observed that CAT and SOD activities increased again in some exposure groups compared to the control. Exposure time also had an effect on biomarkers in different levels. According to the results, the cytotoxicity of DI and MA combination depended on their concentrations. DI or/ and MA, in sub-lethal concentrations, induced oxidative damages in D. polymorpha. Combined exposure of the pesticides can alter their toxicity and may be evidence of increased toxicity and oxidative stress.

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  AtıfScopus3·WoS3·OpenAlex2

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• MakaleAçık Erişim2022Scopus SJRQ2/r/munzur/2956
  The Bioremediation Capacity of Sphingomonas melonis for Methomyl-Contaminated Soil Media: RSM Optimization and Biochemical Assessment by Dreissena polymorpha

  Önder Erguven, Gokhan; Serdar, Osman; Tanyol, Mehtap; Cikcikoglu Yildirim, Nuran; Yildirim, Numan; Durmus, Barbaros

  The bioremediation capacity of S. melonis for methomyl investigated with RSM. Oxidative stress and neurotoxic response determined in D. polymorpha. COD and TOC levels were measured. GSH, MDA levels and CAT, SOD, AChE activities in D. polymorpha exposed to before (group A) and after (group B) bioremediated media during 24 and 96 h were tested by using ELISA kit. AchE activity was decreased 24th h but increased during 96th h in group B compared with group A (p<0.05). MDA levels and CAT activities were decreased during 24 h and 96 h in group B compared with group A (p<0.05). GSH levels were increased during 24 h and 96 h in group B compared with group A (p<0.05). SOD activity did not show a significant change in group B (p<0.05). Methomyl could be effectively remediated by S. melonis and CAT, AChE activities and GSH, TBARS levels of D. polymorpha are useful biomarkers for evaluating the bioremediation capacity.

  AtıfScopus5·WoS6·OpenAlex6

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