Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2026Scopus SJRQ3/r/munzur/1531
  Determination of Oxidative Stress Responses Induced by the Combination of 4 Different Rare Earth Elements in Dreissena polymorpha; [Determinacija odgovorov na oksidativni stres, ki ga povzroča kombinacija 4 različnih redkih zemljinskih elementov v Dreissena polymorpha]

  Serdar, Osman; Aydin, Ayşe Nur

  Rare Earth Elements (REE), whose usage areas are increasing day by day, are increasing in the amount of mixing with the environment, causing changes in antioxidant enzyme activities by causing oxidative stress in living organisms. In this study, it was aimed to examine the oxidative stress responses induced by the mixture of 4 different REEs (terbium, gadolinium, Lanthanum, Praseodymium) in Dreissena polymorpha. For this purpose, sublethal concentration values were determined by literature review. Experimental application was carried out within 24 and 96 hours. In the analyzes performed to determine biomarker responses, samples taken from living organisms were weighed and homogenization processes were performed for the analysis of samples taken from the experimental groups, including the control group. After homogenization, samples were centrifuged at 4.000 rpm for 15 minutes. Supernatants were kept at -86 °C until measurements were made. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities, and glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) levels were determined using ELISA kits. Statistical analyses were performed using SPSS. One-way ANOVA (Duncan’s multiple range test; p < 0.05) was used for comparison of measured parameters among groups. As a result of the application, a decrease in CAT activity and GSH level and an increase in TBARS levels were observed after 96 hours compared to the control group, while no statistically significant difference was detected in SOD and GPx activities.

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• MakaleAçık Erişim2026Scopus SJRQ3/r/munzur/1125
  Oxidative Stress Responses induced by Yttrium in Gammarus pulex at Increasing Temperatures

  Aydın, Ayşe Nur; Serdar, Osman

  In parallel with the increase in the need for raw materials used in industry, the area and amount of use of Rare Earth Elements are also increasing. Yttrium (Y), which is used as a raw material source, is thought to form residues in the environment. Gammarus pulex individuals were selected as a model organism to investigate the effects of Yttrium on the environment and aquatic organisms. G. pulexes were exposed to Y at different temperatures (16, 18, and 20 °C) and concentrations (25, 100, and 500 µg/L) for 96 hours and samples were taken at 24 and 96 hours for biochemical analyses. For biomarker analysis, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities, thiobarbituric acid (TBARS) and glutathione (GSH) levels were performed using CAYMAN ELISA kit. Study data revealed that SOD and CAT activities increased significantly with increasing temperature and exposure concentration, while GPx activity showed an irregular response; in contrast, no significant change was observed in TBARS levels at 16 °C, while the increase in TBARS and significant changes in GSH levels at 18 and 20 °C indicate that oxidative stress becomes more pronounced at high temperatures. Copyright Aydın,, A. N. & Serdar, O.

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• MakaleAçık Erişim2026Scopus SJRQ4/r/munzur/1104
  Oxidative Stress Response to Zinc Oxide, Copper Oxide and Iron (III) Oxide in Gammarus pulex; [Gammarus pulex'te Çinko Oksit, Bakır Oksit ve Demir (III) Oksit'e Karşı Oksidatif Stres Tepkisi]

  Aydin, Ayşe Nur; Çiçek Çi̇Men, Işıl Canan; Serdar, Osman

  Nanoparticles (NPs) are increasingly used across various fields of science and technology, including food science from crop harvesting to final food consumption. With their widespread application in daily life, the environmental effects of nanoparticles have become an important area of academic investigation. In this study, Gammarus pulex species was selected as research material/bioindicator species to investigate the effects of zinc oxide (ZnO), copper oxide (CuO), and iron(III) oxide (Fe2O3) on aquatic life. To conduct the experiments, separate setups were established for each of the nanoparticles (ZnO, CuO, and α-Fe2O3). Following ten organisms for each concentration group (10, 20, and 40 ppm) with corresponding control groups, exposures were performed for 24 and 96 hours. Samples were collected at both time points and biochemical analyses were conducted using homogenization procedures. The results were evaluated statistically. According to the findings, SOD activity increased significantly after exposure to ZnO, CuO, and Fe2O3. CAT activity did not show any change under ZnO NP exposure, whereas a decrease was observed under CuO and Fe2O3 NP exposures. However, a significant increase in TBARS levels and a significant increase in GSH levels were observed following nanoparticle exposure. Based on the study data, exposure to ZnO, CuO, and Fe2O3 appears to induce oxidative stress in G. pulex.

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• Doktora TeziAçık Erişim2025/r/munzur/7270
  Gadolinyumun farklı sıcaklıklarda zebra balıkları (Danio rerio) üzerindeki etkisinin biyobelirteçlerle belirlenmesi

  Ayşe Nur Aydın

  Nadir toprak elementleri (NTE), elektrik ve elektronik, otomotiv, yenilenebilir enerji, tıp ve savunma gibi çeşitli endüstrilerde çeşitli yüksek teknoloji uygulamalarında kullanılır. Bu nedenle, gelişmekte olan ekonomiler, yeşil teknoloji ve Ar-Ge sektörleri gibi çeşitli sektörlerden gelen artan talep nedeniyle küresel pazarda NTE'lere olan talep her geçen gün artmaktadır. Kullanım alanının artmasına paralel olarak çevreye salınan NTE atıkları çevrede ve sucul ekosistemde kalıntı oluşturararak kirliliğe yol açarak zararlı hale gelmektedir. Son yıllarda iklim değişikliği ile birlikte sıcaklık artışında meydana gelen değişimlerin organizmaların hayati faaliyetlerini olumsuz yönde etkilediği bilinmektedir. İklim değişikliğiyle birlikte sıcaklıkların artması sonucu çevreye bulaşan her türlü kirletici maddenin sıcaklıkla beraber etkisinin arttığı düşünülmektedir. Sunulan bu çalışmada, 0 (kontrol), 10, 50 ve 250 mg/L konsantrasyonlarda NTE'leri temsilen seçilen Gadolinyum (Gd)'un artan sıcaklıklarla birlikte (22, 27 ve 32 °C) model organizma Danio rerio'da meydana getirdiği etkilerin biyobelirteçlerle ve genotoksik çalışmalarla incelenmesi amaçlanmıştır. Deneme gruplarından 24 ve 96 saatlerde alınan örnek numuneler supernatantlarında, superoksitdismutaz (SOD), katalaz (CAT) ve glutatyon peroksidaz (GPx) enzim aktiviteleri ile tiobarbitürik asit reaktif maddeleri (TBARS) ve redükteglutatyon (GSH) düzeyleri ile genotoksik yanıt (8-OHdG) değerlendirilmiştir. Çalışma verileri sonucunda elde edilen bulgular SOD, CAT ve GPx enzim aktivitelerinde istatiksel açıdan anlamlı azalmalar; TBARS seviyesinde artışlar ve GSH seviyesinde istatiksel olarak anlamlı azalmalar; 8-OHdG düzeyinde ise istatiksel olarak anlamlı artışlar olduğunu göstermektedir. Yapılan analizler sonucunda elde edilen sonuçlarda uygulama konsantrasyonu ve sıcaklık artışlarının etkili olduğu düşünülmektedir.

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• MakaleYayıncıda açık erişim2025Scopus SJRQ2/r/munzur/2267
  Determination of Toxic Effects of Copper in Navicula cryptocephala var. veneta by Biomarkers and Bioaccumulation Quantification

  Aydın, Ayşe Nur; Serdar, Osman; Ölçülü, Abdüllatif; Çimen, Işıl Canan Çiçek; Ak, Tuba Parlak; Derman, Taner; Sevim, Ayşegül; Yıldırım, Nuran Cikcikoğlu

  As a result of the increased use of metals such as copper (Cu), copper pollution in the environment is also increasing. In order to investigate the effect of copper mixed into the water environment and on aquatic biota, the microalga Navicula cryptocephala var. veneta was chosen as a model organism. N. cryptocephala var. veneta it was aimed to determine the accumulation and elimination amounts and some biomarker responses. Metal accumulation amounts in the organisms used in the study. The amount of metal accumulated in relation to the wet weight mass of the samples was determined using Atomic Absorption Spectophotometry (AAS) mass spectrometry. In the bioassays conducted within the scope of the study, lipid peroxidation (TBARS) and reduced glutathione (GSH) levels, superoxide dismutase (SOD) enzyme activity, glutathione peroxidase (GSH-Px) enzyme activity and catalase (CAT) enzyme activity were determined with an ELISA microplate reader at 24, 48, 72, 96 and 120 hours. According to the study data, it was observed that Cu metal accumulated in Navicula cryptocephala var. veneta and caused oxidative stress in the organism, affecting the organism by causing changes in TBARS, GSH levels and antioxidant enzymes SOD, CAT and GPx activities. Additionally, it was observed that different elimination levels occurred in the application groups.

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  AtıfScopus1·OpenAlex1

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• MakaleYayıncıda açık erişim2025Scopus SJRQ4/r/munzur/2227
  The effect of Dimethoate on oxidative stress and antioxidant responses of Pontastacus leptodactylus; [El efecto del dimetoato sobre el estrés oxidativo y las respuestas antioxidantes de Pontastacus leptodactylus]

  Aydın, Ayşe Nur; Bulut, Hilal; Serdar, Osman

  Dimethoate (DMT) pesticide is one of the chemicals used to protect some agricultural areas from harmful organisms. DMT residues released directly or indirectly to the environment cause serious problems in nature. DMT residues mixed with the aquatic environment adversely affect aquatic organisms and this effect is carried to humans through the food chain. In this study, oxidative stress responses induced by DMT pesticide in Pontastacus leptodactylus were investigated. For this purpose, oxidative stress and antioxidant parameters Thiobarbituric acid reactive substances (TBARS), Glutathione (GSH), Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) caused by dimethoate (DMT) pesticide in P. leptodactylus at 17.5, 35, and 70 mg·L-1 concentrations at 24 and 96 hours were investigated. Results were determined using ELISA kits. No significant difference was observed in GSH levels and SOD activities compared to control. Statistically significant differences were observed between decreases in CAT and GPx activities and increases in TBARS levels. SPSS 24.0 package program one–way ANOVA (Duncan 0.05) was used in the evaluation of biochemical analyzes. As a result, it was determined that DMT caused oxidative stress formation in P. leptodactylus and caused changes in enzyme activities.

  AtıfScopus1·WoS1

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• MakaleYalnız künye2025Scopus SJRQ1/r/munzur/1667
  Effect of light and noise pollution on oxidative stress and proximate composition in Dreissena polymorpha

  Serdar, Osman; Demirtaş Erol, Nida; Aydin, Ayşe Nur

  In this study, it was aimed to determine the effect of sound and light pollution on Zebra mussel (Dreissena polymorpha) by biomarker and nutritional composition. For this purpose, an experimental design was carried out in laboratory conditions where the model was exposed to live sound, light, and combined sound + light parameters for 30 min every hour over 96 h. Changes in the biomarkers superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, as well as glutathione (GSH) and thiobarbituric acid (TBARS) levels, were assessed in the model organism exposed to sound, light, and sound + light pollution. The changes in protein and fat amounts were determined. When the changes in biomarkers were examined statistically compared to the control, there was no significant decrease in SOD activity (p < 0.05), a significant increase in CAT activity in sound and sound+light, but a significant decrease in light (p < 0.05), no significant difference in GPx activity (p > 0.05). It was determined that there was a significant increase in GSH and TBARS levels in all three parameters. Significant changes were also observed in the proximate composition. As a result, exposure to sound, light and sound+light pollution caused an increase in oxidative damage and decreases in protein and lipid composition were detected.

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  AtıfScopus7·WoS6·OpenAlex8

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• MakaleAçık Erişim2025Scopus SJRQ2/r/munzur/1451
  Determination of the Effect of Nanoparticle Copper on Navicula cryptocephala var. veneta by Biomarkers and Bioaccumulation Amounts

  Serdar, Osman; Aydın, Ayşe Nur; Ölçülü, Abdüllatif; Çiçek Çimen, Işıl Canan; Parlak Ak, Tuba; Derman, Taner; Pala, Ayşegül; Yıldırım, Nuran Cikcikoğlu

  Pollutants such as copper nanoparticles (CuNP), which form residues in the environment, especially in water environments, cause serious damage to the environment and the organisms living in the environment. In this study, Navicula cryptocephala var. veneta was selected as a model organism. The EC50 values of CuNP on Navicula cryptocephala var. veneta were determined as 4.94 mg\L and 3 different application concentrations were determined considering this ratio. The experiments were carried out in 3 replicates and samples were taken at 24 and 96 hours. At the end of 120 hours, samples were taken to determine the elimination values. CuNP accumulation amounts in the samples were measured by electrothermal atomic absorption spectrophotometer (ETAAS). For biomarker analysis, thiobarbituric acid (TBARS) and glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were performed using CAYMAN ELISA kit. According to the study data, as CuNP concentration and application time increased, the amount of accumulation in Navicula cryptocephala also increased. Increasing-decreasing changes were observed in elimination. CuNP caused oxidative stress in N. cryptocephala and caused changes in TBARS and GSH levels. In addition, it caused increases in SOD activities and statistically significant decreases (P<0.05) in CAT and GPx activities.

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  AtıfScopus2·WoS1

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• MakaleAçık Erişim2024/r/munzur/6359
  Terbiyumun Pontastacus leptodactylus’ta ki Oksidatif Stres ve Antioksidan Yanıtlarının Belirlenmesi

  Serdar, Osman; Aydın, Ayşe Nur

  Terbiyum (Tb), su ortamında yüksek konsantrasyonlarda bulunduğunda sucul organizmalar için toksik hale gelebilen nadir toprak elementi (NTE)’dir. Tatlı su ekosistemindeki Tb toksisitesinin meydana getirdiği oksidatif stres sonuçlarıyla ilgili hala yeterince çalışmalar mevcut değildir. Bu çalışma ile Tb'nin oksidatif stres sonuçlarını değerlendirmek için tatlı su canlısı olan Pontastacus leptodactylus kullanılmıştır. Bu organizmada, 1, 5 ve 25 mg/L konsantrasyonlardaki Tb'nin 24 ve 96. saatte meydana getirdiği oksidatif stres ve antioksidan değişimleri süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px), katalaz (CAT) enzim aktiviteleri ile Tiyobarbitürik asit (TBARS) ve redükte glutatyon (GSH) seviyeleri belirlenmiştir. Sonuçlar ELISA kitleri kullanılarak tespit edilmiştir. Biyobelirteç analizlerin istatatistiksel değerlendirilmesinde SPSS 24.0 paket program tek-yönlü ANOVA (Duncan 0,05) testi kullanılmıştır. Kontrol grubu ile karşılaştırıldığında SOD aktivitesinde önemli bir değişim gözlemlenmezken (p>0,05) CAT ve GPx aktivitelerinde anlamlı azalmalar (p<0,05) gözlemlenmiştir; GSH ve TBARS seviyelerinde ise anlamlı (p<0,05) artışlar gözlemlenmiştir. Çalışma verileri dikkate alındığında canlı organizma vücuduna nüfuz eden Tb ksenobiyotiğinin organizma da oksidatif strese neden olduğu düşünülmektedir.

  AtıfOpenAlex4·TR Dizin3

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• MakaleAçık Erişim2024/r/munzur/6352
  Effect of Probiotic on Copper Nanoparticle Accumulation in Dreissena polymorpha

  Serdar, Osman; Aydın, Ayşe Nur

  Materials with dimensions between 0.1 and 100 nm are called nanoparticle (NP) materials. In recent years, the usage areas and quantities of NP materials have increased in parallel with the development of the industry. The need and usage areas of heavy metals such as Cu have also expanded in NP sizes. All these developments have led to problems on the ecosystem that are becoming more difficult to compensate. In this study, Zebra Mussel (Dreissena polymorpha) was chosen as a model to investigate the effect of probiotics on CuNP heavy metal accumulation. The model organism was exposed to three different concentrations of CuNP (5, 10, 50 mg/L) with probiotics and directly for 24 and 96 hours. CuNP accumulation amounts in D. polymorpha tissues treated directly and with probiotics were compared. The amount of accumulation in the test organism directly exposed to CuNP was higher compared to the groups administered with probiotics, but a statistically significant difference (p&lt;0.05) was found only in the treatment group with the highest 24-hour concentration (50 mg/L). As a result, according to the findings obtained from the study, it has been determined that probiotics have positive developmental effects on aquatic organisms, as well as beneficial in the elimination of their accumulation in the organism.

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  AtıfOpenAlex4·TR Dizin1

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