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• MakaleYalnız künye2023Scopus SJRQ2/r/munzur/2713
  Isolation, morphological identification, and xylanase characteristics of anaerobic gut fungi Neocallimastix from Anatolian wild goat

  Kar, Bülent; Torcan, Berat

  This study shows the morphological identification of anaerobic fungal strains isolated from fecal samples of goats inhabiting Turkey and the effects of various metal ions and chemicals on extracellular xylanase production. Three different anaerobic gut fungi isolated from wild goats in Turkey were identified as Neocallimastix spp. xylanase, cellulase, and lichenase production were tested in culture supernatants, and the maximum-specific activities were found as 560.42 ± 9.39, 159.70 ± 3.88, and 157.36 ± 3.83 (μmol/min/mg protein), respectively. While the optimum temperature range of exo-xylanases was found as 40–50°C, their optimum pH range was determined as 6.0–6.5. Xylanase activity decreased in metal ions and other chemical reactants based on dose. The metal ion that significantly inhibited xylanase activity was Fe+3. It was found that the ferric ions inhibited xylanase activity in all three anaerobic gut fungi by 30%–90% depending on molarity. On the contrary, the 1 mM concentrations of the Mn+2, Ba+2, Co+2, Cu+2, Sn+2, and Mg+2 metal ions and the ethylenediaminetetraacetic acid and β-mercaptoethanol reagents had a positive effect at rates in the range of 3%–92%. In conclusion, these findings demonstrate that anaerobic gut fungus has very stable fibrolytic enzymes that need to be separated, as well and the existence of a unique resource for industrial applications.

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• MakaleYalnız künye2023Scopus SJRQ2/r/munzur/2104
  Antibacterial activity of novel synthesized chitosan-graft-poly(N-tertiary butylacrylamide)/neodymium composites for biomedical application

  Yazdıç, Ferit Can; Karaman, Altuğ; Torğut, Gülben; Ayhan, Nagihan Karaaslan

  In this present study, composites of chitosan-graft-poly(N-tertiary butylacrylamide) (CH-graft-poly(N‑tert‑BAAm)) copolymer, with Neodymium (Nd), an important rare earth element, were prepared by precipitation technique. Nd was successfully incorporated into the polymer of different weight percentages (0.5%, 1%, and 2%) without any degradation. The effect of neodymium additives on the structural, morphological, and antibacterial activities against gram-positive bacteria and gram-negative bacteria of the polymer was analyzed using various instrument techniques. X-ray diffraction (XRD) results together with Fourier Transform Infrared (FT-IR), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) studies confirmed the morphology of Nd-doped CH-graft-poly(N‑tert‑BAAm) composites without any other impurities. The antibacterial effect of Nd was studied by adding it to the copolymer in a weight ratio of 0.5%–2%. The antibacterial effect of neodymium concentration on four different strains of bacteria was investigated: Escherichia coli (ATCC 25922) (E. coli), Pseudomonas aeruginosa (DSM 50071) (P. aeruginosa), Bacillus subtilis (DSM 1971) (B. subtilis), and Staphylococcus aureus subsp. aureus (ATCC 25923) (S. aureus). The antibacterial activities of the obtained composites were determined using the Agar Well Diffusion Assay Method. Experimental results show that Nd binds well to CH-graft-poly(N‑tert‑BAAm). Activity against E. coli, P. aeruginosa, B. subtilis, and S. aureus subsp. aureus creates a potential for pharmaceutical and biomedical applications.

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  AtıfScopus8·WoS7·OpenAlex8

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