Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2022Scopus SJRQ4/r/munzur/2645
  The role of A268V exon-7 polymorphism of PPARA in development of axial spondyloarthritis; [Aksiyal spondiloartrit gelişiminde PPARA'nın A268V ekson-7 polimorfizminin rolü]

  Akbulut, Ekrem; Yolbas, Servet; Ozgen, Metin

  Objectives: Axial spondyloarthritis (axSpA) is a chronic inflammatory disease that mainly affects the axial skeleton. Peroxisome proliferator activated receptor alpha (PPARA) is an intracellular transcription factor, which play a role in inflammation and osteoblasting activity. This study is designed to investigate the relationship of NG_012204.2:p.Ala268Val polymorphism of PPARA with axSpA risk and its role in disease development. Methods: This study was conducted with 168 patients and 181 controls. Genotyping was done with MALDITOF. Gene expression level was analyzed by quantitative real time PCR (RT-qPCR). The protein homology models of PPARA were created with ProMod3. Ligand binding dynamics were tested using the AutoDock4 docking program. Statistical evaluations were made with SPSS (ver24) and GeneGlobe. Results: Our results showed that C>T polymorphism causing NG_012204.2:p.Ala268Val change was associated with disease risk (p=0.024) and T allele increased disease risk 1.7 times (95% CI=1.070-2.594). PPARA expression decreased (p<0.05) in individuals carrying the T allele. We determined that the ligand entry pocket was opened 1.1 Å in the polymorphic PPARA. Polymorphic change caused a decrease in the ligand binding affinity. Conclusions: Our results provide an important contribution to elucidating the development of axSpA and demonstrate the potential of PPARA as a marker for the diagnosis of axSpA.

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  İncele
• MakaleAçık Erişim2020/r/munzur/3341
  Contribution of Polymorphism in PPARα Untranslated Region to The Development of Axial Spondyloarthritis; [PPARα Kodlanmayan Bölge Polimorfizminin Aksiyal Spondiloartrit Gelişimine Katkısı]

  Akbulut, Ekrem; Özgen, Metin

  The study aimed to contribute to the overall understanding of axial spondyloarthritis by investigating the polymorphism in 5’ untranslated region of peroxisome proliferator-activated receptor alpha gene in patients. The study included 194 patients and 197 controls recruited. The DNA obtained from the samples was genotyped by multiplex Polymerase Chain Reaction and then Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. Data were analyzed by logistic regression. Five polymorphic regions analyzed in this study, three of the sites were associated with disease risk. An allele in the rs1800204 polymorphic region (p < 0.001), C allele in the rs4253657 polymorphic region (p = 0.040) and C allele in the rs13909022 polymorphic region (p = 0.005) were associated with disease risk. The association of disease risk could not be detected with G and A alleles in the rs881740 (p = 0.456) and rs115640476 (p = 0.674) polymorphic regions, respectively. PPARα 5' untranslated region polymorphism, which is shown to be associated with disease risk, is thought to contribute to the elucidation of the molecular mechanism of the disease. In axial spondyloarthritis, studying the effects of genetic changes in PPARα on other genes with which it interacts will contribute to the full understanding of the molecular mechanism of the disease.

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  AtıfScopus1·OpenAlex1

  İncele
• MakaleAçık Erişim2016Scopus SJRQ2/r/munzur/3981
  Comprehensive structural analysis of the open and closed conformations of Theileria annulata enolase by molecular modelling and docking

  Mutlu, Ozal; Yakarsonmez, Sinem; Sariyer, Emrah; Danis, Ozkan; Yuce-Dursun, Basalt; Topuzogullari, Murat; Akbulut, Ekrem; Turgut-Balik, Dilek

  Theileria annulata is an apicomplexan parasite which is responsible for tropical theileriosis in cattle. Due to resistance of T. annulata against commonly used antitheilerial drug, new drug candidates should be identified urgently. Enolase might be a druggable protein candidate which has an important role in glycolysis, and could also be related to several cellular functions as a moonlight protein. In this study; we have described three-dimensional models of open and closed conformations of T. annulata enolase by homology modeling method for the first time with the comprehensive domain, active site and docking analyses. Our results show that the enolase has similar folding patterns within enolase superfamily with conserved catalytic loops and active site residues. We have described specific insertions, possible plasminogen binding sites, electrostatic potential surfaces and positively charged pockets as druggable regions in T. annulate enolase. (C) 2016 Elsevier Ltd. All rights reserved.

  AtıfWoS8·OpenAlex8

  İncele
• BildiriAçık Erişim2016Scopus SJRQ1/r/munzur/4379
  Comprehensive computational analysis of Theileria annulata enolase by comparative modeling and docking

  Mutlu, Ozal; Yakarsonmez, Sinem; Sariyer, Emrah; Danis, Ozkan; Dursun, Basak Yuce; Topuzogullari, Murat; Akbulut, Ekrem; Turgut-Balik, Dilek

  İncele
• MakaleYalnız künye2015/r/munzur/4457
  GENOTYPING AND ANALYSIS OF rs27037 POLYMORPHISM FOR ANKYLOSING SPONDYLITIS

  Akbulut, Ekrem; Erdemir, Aysegul; Ozgen, Metin; Baskin, Yasemin; Yalcin, Femin; Balik, Dilek Turgut

  İncele