Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2022Scopus SJRQ4/r/munzur/2645
  The role of A268V exon-7 polymorphism of PPARA in development of axial spondyloarthritis; [Aksiyal spondiloartrit gelişiminde PPARA'nın A268V ekson-7 polimorfizminin rolü]

  Akbulut, Ekrem; Yolbas, Servet; Ozgen, Metin

  Objectives: Axial spondyloarthritis (axSpA) is a chronic inflammatory disease that mainly affects the axial skeleton. Peroxisome proliferator activated receptor alpha (PPARA) is an intracellular transcription factor, which play a role in inflammation and osteoblasting activity. This study is designed to investigate the relationship of NG_012204.2:p.Ala268Val polymorphism of PPARA with axSpA risk and its role in disease development. Methods: This study was conducted with 168 patients and 181 controls. Genotyping was done with MALDITOF. Gene expression level was analyzed by quantitative real time PCR (RT-qPCR). The protein homology models of PPARA were created with ProMod3. Ligand binding dynamics were tested using the AutoDock4 docking program. Statistical evaluations were made with SPSS (ver24) and GeneGlobe. Results: Our results showed that C>T polymorphism causing NG_012204.2:p.Ala268Val change was associated with disease risk (p=0.024) and T allele increased disease risk 1.7 times (95% CI=1.070-2.594). PPARA expression decreased (p<0.05) in individuals carrying the T allele. We determined that the ligand entry pocket was opened 1.1 Å in the polymorphic PPARA. Polymorphic change caused a decrease in the ligand binding affinity. Conclusions: Our results provide an important contribution to elucidating the development of axSpA and demonstrate the potential of PPARA as a marker for the diagnosis of axSpA.

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  İncele
• MakaleAçık Erişim2019Scopus SJRQ4/r/munzur/3608
  Investigation of relaxation times in 5-fluorouracil and human serum albumin mixtures

  Korunur, Sibel; Zengin, Bilgin; Yilmaz, Ali

  Background: Human serum albumin (HSA) is often selected as a subject of any study because albumin is the most abundant protein in human blood plasma. NMR is recognized as a valuable method to determine the structure of proteins-ligand and protein-drug complexes. Objective - Aim of the study: In this study, protein drug interactions were investigated using 5-Fluorouracil anticancer drug and human serum albumin protein. Materials and methods: In this context 400 MHz NMR spectrometry was used and NMR relaxation rates in drug-albumin complex were investigated with respect to increase albumin concentration and increase in 5-Fluoro-uracil (5-FU)-albumin solution temperature. Results: The results of this study indicated that 5-FU had a weak association with albumin, and it easily dissociated from the protein to which it was attached. Conclusion: The obtained results also gave us useful information about molecular dynamics of drug-albumin interactions.

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  AtıfScopus4·WoS3·OpenAlex4·TR Dizin1

  İncele
• MakaleYalnız künye2010Scopus SJRQ3/r/munzur/4319
  Investigation of Some Antioxidant Enzymes Activities Depending on Adrenomedullin Treatment and Cold Stress in Rat Liver Tissue

  Yildirim, Nuran Cikcikoglu; Yürekli, Muhittin; Yildirim, Numan

  Purpose: The aim of this study is to investigate the compensating effects of adrenomedullin on liver tissue after cold stress treatment. Materials and Methods: Male Wistar rats were divided into four groups as control, adrenomedullin, cold stress and cold stress + adrenomedullin treated. Adrenomedullin was given by intraperitoneal injection (2 mg/g body weight) once a day for a week. For cold stress the rats were kept at 10 C for a week. Catalase, glutathione peroxidase and superoxide dismutase activities were measured in liver tissue. Results: Catalase activity was decreased significantly as compared to control in adrenomedullin treated group (p<0.05). There was no significant difference in superoxide dismutase activity (p>0.05). Glutathione peroxidase activity was increased in adrenomedullin treated group but this was statistically insignificant (p>0.05). All enzyme activities were decreased by cold stress (p<0.05). In Adrenomedullin+cold stress group, only the glutathione peroxidase activity was significantly decreased (p<0.05). Conclusion: It is concluded that cold stress induces decrease in catalase, superoxide dismutase and glutathione peroxidase activities in rat liver. Adrenomedullin treatment corrected only glutathione peroxidase activity while decreasing catalase and superoxide dismutase activities further. The discrepancy may be explained by different responses of antioxidant systems to oxidative stress. It can be concluded that oxidative factors may display unexpected effects on the regulation of glutathione peroxidase activities in the adrenonmedullin case.

  AtıfWoS8

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