Sürdürülebilir Kalkınma Amaçları

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• MakaleYalnız künye2022Scopus SJRQ3/r/munzur/2929
  Ascorbic acid ameliorated the sperm quality of rainbow trout (Oncorhynchus mykiss) against arsenic toxicity: Impact on oxidative stress, fertility ability and embryo development

  Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Aksu, Önder; Çakir Sahilli, Yeliz

  Arsenic (As) is a heavy metal and aquatic pollutant and adversely impacts the reproduction of male fish. As a chain-breaking antioxidant, ascorbic acid (AA) has high water solubility and low toxicity. In this context, the current study was performed to assess the protective role of AA (1 mM) on the sperm cells of the rainbow trout (Oncorhynchus mykiss) exposed to sublethal concentrations of As (8, 16 and 32 mg/L). Sperm quality parameters were analyzed using a sperm class analyzer system. Lipid peroxidation and antioxidant enzyme levels were used as indicators of oxidative stress. The fertilization, eyeing and hatching rates were determined as gamete markers. Reduced sperm quality parameters and fertility capacity resulted from in vitro exposure to As (P < 0.05). The oxidative stress in sperm cells increased after As exposure (P < 0.05). The presence of AA improved sperm movement parameters and fertility potential (P < 0.05). Overall, AA had a positive effect on oxidative stress and fertility ability against As toxicity and AA supplementation ameliorated detrimental effects of As in sperm cells.

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  AtıfScopus11·WoS9·OpenAlex5

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• MakaleYalnız künye2021Scopus SJRQ3/r/munzur/2827
  Glyphosate disrupts sperm quality and induced DNA damage of rainbow trout (Oncorhynchus mykiss) sperm

  Akça, Ayşe; Kocabaş, Mehmet; Kutluyer, Filiz

  As a widespread pollutant, glyphosate (GLY) adversely affects the aquatic environment and can impair the reproductive ability and functions of fish. The purpose of the current study was to assess in vitro effect of GLY on rainbow trout (Oncorhynchus mykiss) sperm cells. The sperm cells were exposed to different GLY concentrations (2.5, 5, 10 mg/L). Sperm motility parameters were analyzed with computer assisted sperm analysis. DNA fragmentation (%) was measured by the comet assay using fluorescence microscopy. With increased GLY concentration, sperm motility and duration decreased after exposure. DNA fragmentation (% DNA in tail) in sperm cells was higher in treatments containing GLY than control (p < 0.05). Consequently, sperm cells are sensitive to low doses of GLY, and this can negatively affect natural populations.

  AtıfScopus8·WoS4·OpenAlex5

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