Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2026/r/munzur/6062
  Seasonal changes in oxidant and antioxidant biomarkers in liver and muscle of common carp (Cyprinus carpio) in Atatürk Dam Lake

  Benzer, Fulya; Tepe, Rıdvan; Erisir, Mine; Turkoglu, Semra; Fırat, Özgür; Şahin, Ayse Gül; Sünbül, Muhammet Raşit; Örnekci, Gürel Nedim; Küçükyılmaz, Mehmet; Öz, Özden Barım

  Seasonal investigations were conducted on 640 Cyprinus carpio specimens obtained through commercial fishing at four different stations (Gerger, Sitilce, Hosirge, and Samsat) in the Atatürk Dam Lake. MDA (malondialdehyde) levels were measured in the liver and muscle tissues of male and female fish; the results revealed a significant increase in MDA levels at stations exposed to point-source discharge. This indicates that pollution-induced oxidative stress in fish varies significantly across seasonal and regional scales. Among the stations compared, the lowest MDA values were detected in fish tissues from the Gerger station, while the highest values were recorded at the Sitilce and Hosirge stations. Analysis of SOD, GSH-Px, and GSH values across all stations showed that these parameters fluctuated in correlation with MDA levels thereby maintaining the oxidant-antioxidant balance. This study investigated seasonal and spatial variations in oxidative stress biomarkers in common carp (Cyprinus carpio) collected from four stations in Atatürk Dam Lake. A total of 640 specimens were analyzed for malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and reduced glutathione (GSH) levels in liver and muscle tissues. Results showed that MDA levels were significantly higher in fish collected from Sitilce and Hosirge stations, indicating elevated lipid peroxidation associated with point-source pollution. In contrast, the lowest MDA levels were observed at the reference station, Gerger. Antioxidant enzyme activities (SOD, GSH-Px, and GSH) exhibited tissue-, season-, and site-dependent variations, suggesting adaptive responses to oxidative stress. Overall, the findings demonstrate that anthropogenic pressures in Atatürk Dam Lake significantly alter the oxidant–antioxidant balance in fish, highlighting the usefulness of these biomarkers for environmental monitoring.

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• MakaleYayıncıda açık erişim2026Scopus SJRQ2/r/munzur/1236
  Sperm cryopreservation of sex-reversed rainbow trout (Oncorhynchus mykiss): incorporation of amino acids and anti-oxidants to the extender media to improve the anti-oxidant system

  Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Benzer, Fulya; Erişir, Mine

  Anti-oxidants are vital for protecting sperm and can mitigate the negative effects associated with cryopreservation. This study was conducted to evaluate the protective effects of adding anti-oxidants (vitamins and amino acids) to extender in sex-reversed rainbow trout (Oncorhynchus mykiss) sperm. The collected sperm was diluted at the ratio of 1: 5 by the extenders supplemented with different anti-oxidants including 1.00 mM of ascorbic acid and L-tryptophan, 2.00 mM of cysteine and α-tocopherol, and their combination. After dilution, the semen was aspirated into 0.50 mL straws, and the straws were placed on the tray, frozen for 10 min, and plunged into liquid nitrogen. Straws were thawed in a 30.00 ˚C water bath for 15 sec. The Sperm Class Analyzer System was used to evaluate sperm kinematics. The activity of anti-oxidant enzymes and lipid peroxidation were determined as oxidative stress indices. Our data indicated that the incorporation of anti-oxidants and amino acids increased sperm motility duration. The elevated activity of glutathione peroxidase, superoxide dismutase, and catalase in post-thaw samples indicates that the anti-oxidant system in sex-reversed rainbow trout sperm likely plays a crucial role in protecting membrane compounds from oxidation. In conclusion, the combination of 1.00 mM L-tryptophan and ascorbic acid to the extender media caused a prolonging effect in sperm motility after thawing and they have the potential to serve as effective agents for improving sperm cryosurvival.

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• MakaleAçık Erişim2026Scopus SJRQ4/r/munzur/774
  INTEGRATED BIOLOGICAL TREATMENT AND ELECTROCOAGULATION FOR SLAUGHTERHOUSE WASTEWATER: A NOVEL APPROACH USING FRESHWATER SNAILS (Viviparus contactus, Planorbis rubrum, Melanoides tuberculata)

  Öğedey, Ayşenur; Kocabaş, Filiz Kutluyer; Kizak, Volkan; Kocabaş, Mehmet; Benzer, Fulya; Erişir, Mine; Öğedey, Hayrullah

  As a potential pollution source, slaughterhouse wastewater (SWW) with development of the meat production and processing sector is a threat in the world. Therefore, the determination of economical, favourable, cost-effective and proper treatment method is crucial for sustainable environmental protection. This study focused on the performance and efficiency of a two-stage process combining biological treatment (BT) with freshwater snails and electrocoagulation (EC) of SWW. Viviparus contectus, Planorbis rubrum, Melanoides tuberculata were used in primary treatment while secondary treatment was realized with EC process. The EC of SWW was performed for reducing both turbidity and chemical oxygen demand (COD) concentration to levels compliant with direct-discharge regulations. In addition, the stirring speed, current density, initial pH and reaction time were assessed for evaluation the optimum EC condition. Oxidative stress biomarkers and some antioxidants were assessed in freshwater snails exposed to SWW after 24 and 96 h. The best removal efficiencies of COD (91.43%) and turbidity (92.42%) were obtained at pH 5 and 20 min of operational time by P. rubrum. Notably, MDA (Malondialdehyde) was highest level in M. tuberculata compared to V. contectus and P. rubrum. Overall, removing organic and inorganic pollutants from SWW could be provided with the hybrid process BT/EC as a combining approach.

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• MakaleAçık Erişim2025/r/munzur/6184
  Determination of Total Phenolic Content, Phenolic Composition, Antioxidant Capacities and Some Physicochemical Features of Rosehip (Rosa canina) and Hawthorn (Crataegus Orientalis) Wild Fruits Growing in Tunceli

  Benzer, Fulya; Karakavuk, Emrah; Eroğlu, Zeynep; Çetin, Şenay Kasun

  The role of natural antioxidants is increasingly important in reducing the damaging effects of free radicals formed in daily life and due to various factors in our en-vironment. In this respect, hawthorn and rosehip fruits, which have high amounts of antioxidants and phenolic substances, attract attention. In this study, some phys-icochemical properties, antioxidant activities, total phenolic substance amounts (TFMM) and phenolic compound compositions of rosehip (Rosa canina) and hawthorn (Crataegus orientalis) fruits growing naturally in Tunceli province were investigated. TFMM was determined as 1265.02-1344.68 in rosehip fruits grown in Tunceli and 318.18-370.85 mg GAE kg-1 fresh weight in hawthorn fruits, antiox-idant activity was determined as 4.83-5.26 in rosehip fruits and 1.85-2.36 μmol AAE g-1 fresh weight in hawthorn fruits. A statistical difference was determined between rosehip and hawthorn wild fruits in terms of antioxidant activity and TFMM, and it was observed that rosehip fruit had higher values than hawthorn fruit in terms of both parameters. It was determined that hawthorn fruit contained higher amounts of epigallocatechin, protocatechuic acid and quercetin-3-glucoside phenolic compounds than rosehip fruit and the difference between the two fruit contents was statistically significant.

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• MakaleAçık Erişim2025Scopus SJRQ3/r/munzur/1777
  l-Tryptophan improves the sperm quality of threatened Abant trout (Salmo abanticus) against glyphosate toxicity: impact on oxidative stress and DNA damage

  Kocabaş, Mehmet; Kocabaş, Filiz Kutluyer; Erişir, Mine; Benzer, Fulya; Arslan, Halil Ozancan; Keleş, Esin; Aksu, Önder

  The current study focused on the ameliorating impact of TRP (5 mM) on Salmo abanticus spermatozoa exposed to sub‑lethal concentrations of GLY (2.5, 5, and 10 mg/L). Sperm quality parameters, oxidative stress indices, and DNA damage were analyzed in the post‑exposure sperm. Our data reveal that GLY has an adverse effect on motility rate and duration, sperm kinetics parameters, antioxidant status and DNA damage, and triggers antioxidant defense mechanisms at the highest dose (10 mg/L) and it has insufficient therapeutic effect. Overall, GLY poses potential hazards to male reproductive cells and may adversely affect subsequent generations of natural populations.

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• MakaleAçık Erişim2025Scopus SJRQ3/r/munzur/1385
  Bioactive Compounds and Antioxidant Properties of Mucus from Freshwater Snails Viviparus contectus and Planorbis rubrum

  Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Kızak, Volkan; Korkmaz, Büşra; Benzer, Fulya; Erişir, Mine

  Abstract: As a viscous-elastic substance, snail mucus has a great attention in the medical and cosmetic industries in recent years due to contain many bioactive compounds. Freshwater snails are cosmopolite aquatic invertebrates and distribute nearly all types of inland aquatic bodies due to their adaptive abilities, rapid growth and short life cycles and, the compounds of mucus derived from freshwater snails has not been well documented. Hence, this study focused on identification bio-active ingredients and determination of protein content and antioxidant enzyme activities in freshwater snails Viviparus contectus and Planorbis rubrum mucus. Bioactive constituents of mucus in freshwater snails were characterized by Gas Chromatography-Mass Spectrometry (GC-FID-MS) analysis. Antioxidants (GSH, SOD, CAT and GSH-Px) and protein content were assessed in freshwater snails. Our data revealed that thirteen compounds are characterized with nonadecanoic acid methyl ester (11.57%), nonadecane (5.01%) and heptadecanoic acid methyl ester (4.19%) as the main components of P. rubrum mucus while eleven compounds are characterized with farnesol (11.23%), 5-hydroxymethyl-1,1,4α-Trimethyl-6-methylene-decahydronphthalen-2-ol (9.73%), kaur-16-en-18-oic acid methyl ester (9.64%), tricosane (9.62%), and kaurene (8.44%) as the main components of V. contectus mucus. Nonadecanoic acid methyl ester and nonadecane, known as effective bioactive constituents having antimutagenic and antitumor capability, were determined with 11.57 and 5.01% of the total bio-active composition of P. rubrum mucus, respectively. As a major constituent, farnesol was detected with 11.23% of the total bio-active composition of V. contectus mucus. Moreover, the presence of kaurene, known as a potent bioactive compound for cancer treatment, in notably quantity in V. contectus mucus. In addition, GSH levels and SOD, CAT, and GPx activities was highest level in P. rubrum compared to V. contectus. Protein content was highest level in V. contectus than P. rubrum. Overall, individual compounds of V. contectus and P. rubrum mucus could be isolated and purified and used for the treatment of a variety of human ailments.

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• MakaleYalnız künye2023/r/munzur/6482
  The effect of boiling and baking process on total phenolic compounds and antioxidant capacity of Osmanoğlu and Sarıaşlama chestnuts grow in Bursa

  Benzer, Fulya; Karakavuk, Emrah; Eroğlu, Zeynep; Eymir, Aslı

  Chestnut is a fruit with high carbohydrate content, low-oil ratio and protein content, including minerals, vitamins, amino acids and phenolic compounds which can have antioxidant properties. It has been determined that the amount and composition of phenolic components could change in according to environmental and growing conditions, and the variety of chestnuts having impact on protein content. This study was conducted to investigate the possible effects of boiling and baking on some physical and chemical properties, antioxidant activity and total phenolic content of Osmanoğlu and Sarıaşlama chestnut varieties grown in Bursa. According to the results of the present study, some physical and biochemical properties of raw chestnut were determined as humidity 48.78–56.57 %, ash 1.11–1.27 g 100 kg⁻¹, water activity 0.705–0.844, pH 7.03–7.29, color L* 60.52±2,64; a* 0.13±0,18; b* 12.39±1.57. The total phenolic compounds content was 129.17±6.23 mg GAE (gallic acid equailent) kg⁻¹ in raw chestnuts, 180.97±18.25 mg GAE kg⁻¹ in boiled chestnuts, and 149.86±7.95 mg GAE kg⁻¹ in baked chestnuts. Also, the antioxidant capacity found like 0.42±0.01 μmol AAE (ascorbic acid equivalent) kg⁻¹ for raw chestnuts, 0.31±0.03 μmol AAE kg⁻¹ for boiled chestnuts, and 0.40±0.01 μmol AAE g⁻¹ of baked chestnuts. It was determined that while the application of the boiling process caused a significant decrease in the antioxidant activity of chestnuts, the application of the baking process did not cause any meaningful change on the antioxidant capacity of the chestnuts. It was determined that there was a significant increase in the total phenolic compounds content with the boiling and baking process compared to raw chestnuts.

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• MakaleAçık Erişim2022Scopus SJRQ3/r/munzur/2829
  Effects of kefir supplementation on body antioxidant-systems, haemolymph heat-shock protein 70 and trypsin, in the crayfish Astacus leptodactylus Eschscholtz (Decapoda, Astacidea)

  Aksu, Önder; Türkoǧlu, Semra; Can, Erkan; Erişir, Mine; Kocabaş, Mehmet; Kayim, Murathan; Benzer, Fulya

  This study was designed to investigate the effects of dietary kefir on trypsin, heat shock protein 70 (HSP70) as well as the oxidant and antioxidant status of the freshwater crayfish, Astacus leptodactylus. The experiment was performed with the four following treatments: Control group, D1, D2 and D3 (kefir supplemented diet 2, 4, 8 g/kg of crayfish body mass, respectively). Crayfish were stocked into 12 fibreglass tanks (three replicates per treatment) and fed for 45 days. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and catalase (CAT) in hepatopancreas and muscle were analysed to determine the status of the oxidant/antioxidant balance. In addition, HSP70 and trypsin were measured in haemolymph. Our results indicated that the activity of GSH-Px, MDA and GSH levels decreased in hepatopancreas ( p > 0.05) and muscle tissues ( p < 0.05) with increasing kefir concentration, while CAT activity increased ( p < 0.05). Trypsin and HSP70 insignificantly decreased in haemolymph.

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  AtıfScopus1·WoS1·OpenAlex1

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• MakaleYalnız künye2022Scopus SJRQ1/r/munzur/2787
  In vivo and in vitro evolution of the effects of cypermethrin on turbot (Scophthalmus maximus, Linnaeus, 1758) spermatozoa

  Beken, Atife Tuba; Saka, Şahin; Aydın, İlhan; Fırat, Kürşat; Suzer, Cüneyt; Benzer, Fulya; Erişir, Mine; Özden, Osman; Hekimoğlu, Müge Aliye; Engin, Serhat

  Synthetic pyrethroid pesticide is commonly used in agricultural activities in the Black Sea region during reproduction period of turbot. In this sense, in vivo and in vitro studies have shown that cypermethrin (CYP) could be one of the environmental factors affecting decreasing turbot stocks. In this study, effects of in vivo and in vitro administration of CYP, a synthetic pyrethroid, on sperm kinematics motility (MOT), progressive motility (PM), curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN), straight line velocity (STR), amplitude of lateral head (ALH), beat cross frequency (BCF), oxidative stress biomarkers malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and lipid peroxidation (LPO) and also histopathological alterations in gonads were investigated in spermatazoa of turbot (Schopthalmus maximus). Broodstock was supplied from culture origin and used in spawning season, additionally, two (0, 0.187 and 0.218 ppb) and three (0, 1.025, 2.05 and 4.1 ppb) different CYP concentrations were performed for in vivo and in vitro studies, respectively. In vivo and in vitro studies, significant reductions were found in sperm MOT, PM, VCL, VSL, VAP, LIN, and ALH properties depend on the increase in CYP concentrations (p < 0.05). Besides, activities of GSH, GPx, SOD, and CAT increased. In terms of histological alterations, no difference was observed among groups (0, 0.187 and 0.218 ppb) in the maturity stage of the germ cells. According to obtained results, sperm kinematics was affected significantly with increased the dose levels of CYP (p < 0.05).

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  AtıfScopus6·OpenAlex8

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• MakaleAçık Erişim2022Scopus SJRQ1/r/munzur/2441
  Aroclor 1254 impairs sperm quality, fertilization ability, and embryo development of rainbow trout (Oncorhynchus mykiss)

  Kocabaş, Mehmet; Kocabaş, Filiz Kutluyer; Aksu, Önder; Benzer, Fulya; Erişir, Mine; Sahilli, Yeliz Çakir

  The polychlorinated biphenyls (PCBs) in aquatic environment adversely affect non-target organisms, including fish. Especially, the male reproduction and next generation can be damaged through high exposure to these pollutants. Hence, the sperm cells were exposed to sublethal concentrations of Aroclor 1254 (0, 1, 5, 10, or 25 mg/l) for 4 h. The sperm quality parameters were analyzed by SCA (Sperm Class Analyzer). The fertility, eyeing, and hatching rates were determined as gamete markers. Lipid peroxidation (malondialdehyde-MDA), glutathione (GSH), and antioxidant enzymes [superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT)] were measured for determination of oxidative stress. Our results showed that Aroclor 1254 negatively affected the motility rate and duration, fertilization rate, embryogenesis, and hatching and also triggered antioxidant defense mechanisms at the highest concentration (25 mg L−1). Furthermore, linear speed (VSL), linearity index (LIN), and amplitude lateral head (ALH) were significantly changed after exposure to 25 mg L−1, and the lowest concentrations (1 and 10 mg L−1) did not significantly affect the motility and fertilizing capacity. The embryogenesis and hatching were significantly affected by sperm exposure to 1, 10, and 25 mg L−1 of Aroclor 1254. Consequently, Aroclor 1254 causes potential hazards in male germ cells, and the exposure of sperm cells to pollutants can adversely affect next generation of wild populations.

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  AtıfScopus6·WoS5·OpenAlex4

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