Sürdürülebilir Kalkınma Amaçları

• 8
• 1
• 1

• MakaleAçık Erişim2025Scopus SJRQ3/r/munzur/2181
  Establishing a link between the chemical composition and biological activities of Gladiolus italicus Mill. from the Turkish flora utilizing in vitro, in silico and network pharmacological methodologies

  Zengin, Gokhan; Cetiz, Mehmet Veysi; Abul, Nurgul; Gulcin, Ilhami; Caprioli, Giovanni; Piatti, Diletta; Ricciutelli, Massimo; Koyuncu, Ismail; Yuksekdag, Ozgur; Bahşi, Muammer

  Objectives: Five solvent extracts (n-hexane, ethyl acetate, ethanol, ethanol/water (70%), and water) of Gladiolus italicus Mill. from Turkey were evaluated for chemical and biological properties. Methods: Antioxidant activities, inhibitory properties against key enzymes involved in the etiology of chronic diseases were tested, as well as cytotoxic effects on different cell lines. Chemical characterization was also carried out to determine the most abundant compounds of each extract. Results: The highest total phenolic content (TPC) was observed in the water extract while highest TFC in ethanol/water extract. The most abundant compounds in the extracts were hyperoside (69041.06 mg kg−1), isoquercitrin (46239.49 mg kg−1), delphindin-3,5-diglucoside (42043.81 mg kg−1), myricetin (21486.61 mg kg−1), and kaempferol-3-glucoside (21199.76 mg kg−1). Molecular dynamic (MD) simulations confirmed the structural stability and dynamic conformational integrity of these complexes over a period of 100 ns. In network pharmacology, A total of 657 unique target genes were screened: 52 associated with programmed cell death-1 (PD-1), 85 with vascular endothelial growth factor receptor-2 (VEGFR2), and 130 with fibroblast growth factor receptor-2 (FGFR2), identifying crucial gene interactions for these proteins. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted, revealing significant interactions and pathways such as the advanced glycation end products (AGE) and their receptors (RAGE) signaling pathway in diabetic complications and T- helper 17 (Th17) cell differentiation, among others. This elucidation of complex networks involving key genes like AKT Serine/Threonine Kinase 1 (AKT1), MYC proto-oncogene (MYC), tumor protein 53 (TP53), Interleukin 6 (IL6), and tumor necrosis factor (TNF) provides a promising foundation for the development of targeted therapies in the treatment of non-communicable diseases. Conclusion: These results show that G. italicus could be a natural source of potent antioxidants and enzyme inhibitors which need to be further explored for the development of biopharmaceuticals.

  • 
  AtıfScopus15·OpenAlex13

  İncele
• MakaleAçık Erişim2024Scopus SJRQ1/r/munzur/2037
  Exploring Chemical Composition, Antioxidant, Enzyme Inhibitory and Cytotoxic Properties of Glaucium acutidentatum Hausskn. & Bornm. from Turkey Flora: A Novel Source of Bioactive Agents to Design Functional Applications

  Yagi, Sakina; Zengin, Gokhan; Uba, Abdullahi Ibrahim; Maciejewska-Turska, Magdalena; Sieniawska, Elwira; Świątek, Łukasz; Rajtar, Barbara; Bahşi, Muammer; Guler, Osman; Dall’Acqua, Stefano

  The present study was performed to determine the chemical constituents, cytotoxicity, antioxidant and enzyme inhibition activities of the aerial parts of Glaucium acutidentatum Hausskn. and Bornm. (family Papaveraceae). Methanolic and aqueous extracts were prepared by maceration, homogenizer-assisted extraction (HAE) and infusion. Results showed that the highest total phenolic and flavonoids contents were obtained from the methanol extracts obtained by HAE (53.22 ± 0.10 mg GAE/g) and maceration (30.28 ± 0.51 mg RE/g), respectively. The aporphine, beznyltetrahydroisoquinoline, and protopine types of Glaucium alkaloids have been tentatively identified. Among them, glaucine was identified in all extracts. Flavonoids, phenolic acids, coumarins, organic acids and fatty acids were also detected. Methanolic extract obtained using the HAE method displayed the highest anti-DPPH (41.42 ± 0.62 mg TE/g), total antioxidant (1.20 ± 0.17 mmol TE/g), Cu2+ (113.55 ± 6.44 mg TE/g), and Fe3+ (74.52 ± 4.74 mg TE/g) reducing properties. The aqueous extracts obtained by infusion and HAE methods exerted the best anti-ABTS (103.59 ± 1.49 mg TE/g) and chelating (19.81 ± 0.05 mg EDTAE/g) activities, respectively. Methanolic extract from HAE recorded the highest acetylcholinesterase (2.55 ± 0.10 mg GALAE/g) and α-amylase (0.51 ± 0.02 mmol ACAE/g) inhibition activities, while that obtained by maceration showed the best butyrylcholinesterase (3.76 ± 0.31 mg GALAE/g) inhibition activity. Both extracts revealed the best tyrosinase inhibitory activity (25.15 ± 1.00 and 26.79 ± 2.36 mg KAE/g, p ≥ 0.05). G. acutidentatum maceration-derived aqueous extract showed selective anticancer activity against cells originating from human hypopharyngeal carcinoma. In conclusion, these findings indicated that G. acutidentatum is a promising source of alkaloids and phenolic compounds for variable pharmaceutical formulations.

  • 
  AtıfScopus13·OpenAlex13

  İncele
• MakaleAçık Erişim2023/r/munzur/6416
  Investigation of the Effects of Ghrelin and Kisspeptin Levels in Liver Tissue of Rats Fed with High Fructose Diet -A Histological Study

  Özay, Yusuf; Güler, Osman; Türk, Ahmet; Karadag, Abdullah; Zencirci, Büşra

  Purpose: High fructose corn syrup (HFCS) is used commonly in the food industry and has been associated with various diseases including obesity, diabetes and fatty liver. Ghrelin, an orexigenic hormone with widespread receptors in many tissues, exerts various central and peripheral effects. Food intake may reduce its synthesis. Kisspeptin plays a major role in reproductive hormones and its receptors are expressed in tissues with high metabolic activity, such as the liver. This study aims to investigate the effects of HFCS consumption on liver tissue and its relationship with Kisspeptin and Ghrelin levels. Materials and Methods: Fourteen adult male Wistar albino rats, aged eight to ten weeks, were used in this study and divided into two groups; Control and HFCS (n=7). Tumor Necrosis Factor-alpha (TNF-α), Ghrelin, and Kisspeptin levels in the liver tissues of the rats were measured using an immunohistochemical method and subsequently analyzed through histoscoring. Results: Our data revealed a significant increase in Kisspeptin, Caspase 3, and TNF-α levels and a decrease in Ghrelin levels in the HFCS group compared to the Control group. Conclusion: In conclusion, our study demonstrates, for the first time, changes in liver Kisspeptin levels induced by a high fructose diet. Furthermore, the consumption of HFCS in food led to inflammatory activation, tissue damage, and reduced Ghrelin levels.

  • 
  İncele
• MakaleAçık Erişim2023Scopus SJRQ2/r/munzur/2297
  Unraveling the chemical profile, antioxidant, enzyme inhibitory, cytotoxic potential of different extracts from Astragalus caraganae

  Kurt-Celep, İnci; Zengin, Gokhan; Uba, Abdullahi I.; Caprioli, Giovanni; Mustafa, Ahmed M.; Angeloni, Simone; Cakilcioglu, Ugur; Guler, Osman; Kaplan, Alevcan; Sharmeen, Jugreet

  Six extracts (water, ethanol, ethanol-water, ethyl acetate, dichloromethane, and n-hexane) of Astragalus caraganae were studied for their biological activities and bioactive contents. Based on high-performance liquid chromatography-mass spectrometry (HPLC-MS), the ethanol-water extract yielded the highest total bioactive content (4242.90 µg g−1), followed by the ethanol and water extracts (3721.24 and 3661.37 µg g−1, respectively), while the least total bioactive content was yielded by the hexane extract, followed by the dichloromethane and ethyl acetate extracts (47.44, 274.68, and 688.89 µg g−1, respectively). Rutin, p-coumaric, chlorogenic, isoquercitrin, and delphindin-3,5-diglucoside were among the major components. Unlike the dichloromethane extracts, all the other extracts showed radical scavenging ability in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay (8.73–52.11 mg Trolox equivalent [TE]/g), while all extracts displayed scavenging property in the 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging assay (16.18–282.74 mg TE/g). The extracts showed antiacetylcholinesterase (1.27–2.73 mg galantamine equivalent [GALAE]/g), antibutyrylcholinesterase (0.20–5.57 mg GALAE/g) and antityrosinase (9.37–63.56 mg kojic acid equivalent [KAE]/g) effects. The molecular mechanism of the H2O2-induced oxidative stress pathway was aimed to be elucidated by applying ethanol, ethanol/water and water extracts at 200 µg/mL concentration to human dermal cells (HDFs). A. caraganae in HDF cells had neither a cytotoxic nor genotoxic effect but could have a cytostatic effect in increasing concentrations. The findings have allowed a better insight into the pharmacological potential of the plant, with respect to their chemical entities and bioactive contents, as well as extraction solvents and their polarity.

  • 
  AtıfScopus31·OpenAlex36

  İncele
• MakaleAçık Erişim2023Scopus SJRQ1/r/munzur/1990
  Screening for Chemical Characterization and Pharmacological Properties of Different Extracts from Nepeta italica

  Acquaviva, Alessandra; Di Simone, Simonetta Cristina; Nilofar; Bouyahya, Abdelhakim; Zengin, Gokhan; Recinella, Lucia; Leone, Sheila; Brunetti, Luigi; Uba, Abdullahi Ibrahim; Guler, Osman

  Plants from the Nepeta genus have been proved to possess different pharmacological properties, among which are antimicrobial, antioxidant, anti-inflammatory, analgesic, and cytotoxic effects. Nepeta italica is a medicinal plant traditionally used for its analgesic effects, and in the present study, the phytochemical composition and biological effects of hexane, dichloromethane (DCM), ethyl acetate (EA), ethanol, ethanol-water, and water extracts of the aerial parts were investigated for determining phenolic composition, antioxidant effects, and anti-inflammatory effects in isolated mouse colon specimens exposed to lipopolysaccharide (LPS). Polar extracts were the richest in terms of phenolic compounds, especially rosmarinic acid. In parallel, ethanol, ethanol-water, and water extracts were also the most effective as scavenging/reducing and enzyme inhibition agents, especially towards cholinesterases and α-glucosidase, and in inhibiting the LPS-induced cyclooxygenase-2 (COX-2) and tumor necrosis factor α (TNFα) gene expression in mouse colon. This poses the basis for future in vivo investigations for confirming the protective effects of polar extracts of N. italica against inflammatory bowel diseases.

  • 
  AtıfScopus31·OpenAlex31

  İncele
• MakaleYalnız künye2022Scopus SJRQ2/r/munzur/2679
  An ethno-veterinary study on plants used for the treatment of livestock diseases in Genç (Bingöl-Turkey)

  Babacan, Ebru Yüce; Polat, Rıdvan; Güler, Osman; Moyan, Ayşe; Paksoy, Mehmet Yavuz; Çakılcıoğlu, Uğur

  There are a very limited number of studies on plants traditionally used in animal diseases in Turkey. As a result, valuable information is getting lost. Traditional plant use in Genç District (Turkey) was recorded by semi-structured interviews with breeders and shepherds. The collected information was subjected to quantitative analysis using the informant consensus factor. It was recorded that a total of 53 plants from 24 families were used in the traditional treatment of animal diseases. 8 of these plants are endemic plants with a narrow distribution area and were recorded for the first time by us. The breeders stated that they applied such plants to animals with peace in mind as these plants had been used for human treatment as well. This study is the first ethnoveterinary study conducted in the province. Phytochemical studies need to be carried out on medicinal plants used in livestock around Bingöl Province. We believe that the use of this preliminary information obtained from ethnoveterinary studies by researchers will contribute to the production of natural animal medicines.

  AtıfScopus32

  İncele
• MakaleAçık Erişim2021Scopus SJRQ2/r/munzur/3025
  An ethnoveterinary study on plants used for the treatment of livestock diseases in the province of Giresun (Turkey)

  Güler, Osman; Polat, Rıdvan; Karaköse, Mustafa; Çakılcıoğlu, Uğur; Akbulut, Sefa

  The lack of ethnoveterinary surveys in Turkey, results in losses in the veterinary phytopharmacology field. This is the first ethnoveterinary research in which statistical calculations about plants are done by informant consensus factor (ICF) method in the Blacksea Region of Turkey. To catalog, analyze, and disseminate the knowledge of plant use in ethnoveterinary practices in the province of Giresun (Turkey). Information was gathered through open and semi-structured interviews with local people, mainly elderly shepherds and farmers, in the Giresun province. The collected data was analyzed quantitatively using two indices, informant consensus factor (ICF). A total of 65 ethnoveterinary plants belonging to 38 families are used for the treatment of animal diseases. According to the local farmers, the most often mentioned species that used in the resarch area have high ethno-veterinary potential. Most of these plants are also used for some human conditions in the study area. There are large numbers of ethno-veterinary practices EVPs in southern Turkey with undocumented cumulative knowledge of the indigenous people. Unfortunately, some evidence of their slow disappearance has been found. The traditional knowledge in the treatment of livestock diseases of the Giresun districts needs further scientific evaluations by phytochemical and antimicrobial experimentation.

  • 
  • 
  AtıfScopus65·WoS54·OpenAlex57

  İncele
• MakaleYalnız künye2020/r/munzur/6796
  The Effect of Propolis on Oxidative Damage Induced by Whole Body Irradiation in Rat

  Baykara, Murat; Erisir, Mine; Özçelik, Mehtap; Güler, Osman

  The antioxidant and radioprotective effects of propolis against ionizing radiation induced oxidativedamage were investigated. Twenty one rats were divided in to 3 groups; 1 st group was the controlgroup; 2nd group was the irradiation group, physiological saline (i.p.)/ 3 days + 9 Gy gammairradiation; 3rd group was irradiation + Propolis group, propolis (i.p. 100 mg / kg BW) / 3 days + 9Gy gamma irradiation. As a result of irradiation, a significant increase was determined inmalondialdehyde (MDA) levels of liver, kidney, and brain tissues of the rats (P<0.001). There was asignificant decrease in glutathione peroxidase (GSH-Px) activity of all the tissues (P˂0.001) andsuperoxide dismutase (SOD) activity and glutathione (GSH) level in the liver (P˂0.01). Also, GSHlevel increased significantly in the kidney (P˂0.001) and ovarium tissues (P˂0.05). The MDA levelsthat increased in the liver as a result of irradiation, decreased significantly due the addition ofpropolis (P˂0.001); whereas, the MDA levels that increased in kidney and brain did not change. Inthe spleen and ovarium tissues, MDA levels decreased significantly as a result of addition ofpropolis compared to the irradiation group (P˂0.05). It was determined that GSH levels, thatdecreased in the liver (P˂0.01), and increased in the kidney due to addition of propolis, and GSHlevels, that decreased in the liver as a result of irradiation (P˂0.01) and increased in the kidney(P˂0.001), approached to the normal level (P˂0.001) and the GSH levels in the spleen and brainincreased compared to both the control and irradiation groups (P˂0.05). The addition of propoliscaused a decrease in the GSH-Px activities of heart, spleen, brain, and ovarium tissues comparedto both the control and irradiation groups (P˂0.001). Also, a decrease in the SOD activity of hearttissue compared to the control group was found (P˂0.05).

  İncele
• MakaleAçık Erişim2020/r/munzur/6747
  The Effect of N-Acetylcysteine on Oxidant/Antioxidant Status in Irradiated Rats

  Baykara, Murat; Özçelik, Mehtap; Erisir, Mine; Guler, Osman

  N-Acetylcysteine (NAC) is both a strong antioxidant and a glutathione precursor. The effect of NAC on the oxidant/antioxidant status of some tissues of the irradiated rats was investigated. Twenty one rats were assigned to 3 groups; the control group, the irradiation group, for which physiological saline solution was administered as i.p. for three days and then, 9 Gy gamma irradiation was applied, and 3rdirradiation + NAC group for which NAC was adminis-tered as i.p. for three days as 300 mg/kg body weight and then the same dose of irradiation was applied. Upon irradia-tion, the increase determined in malondialdehyde (MDA) was significant in the liver, kidney, and brain tissues of the rats (P˂0.05). While glutathione peroxidase (GSH-Px) activity decreased in all of the tissues and superoxide dismutase (SOD) activity only in the liver (P˂0.01), glutathione (GSH) levels significantly increased in the kidney and ovarium tissues (P˂0.001). While NAC administration returned the increased MDA levels in the kidney and brain as a result of irradiation to normal levels (P˂0.05), it was determined that it did not return the increased MDA levels in the liver tissue to the normal level (P˂0.001). While NAC addition led to a significant increase in GSH levels of the liver, heart, spleen, brain and ovarium tissues compared to both control and irradiation groups, it caused a significant decrease in the kid-ney tissue compared to irradiation group (P˂0.001). As a result of NAC addition, a significant decrease was determined in spleen GSH-Px activity, heart and ovarium SOD activity compared to control and irradiation groups (P˂0.05). It can be asserted that GSH increasing by the addition of NAC is the main antioxidant that has a role in decreasing oxidative stress occurring as a result of irradiation. In the examination of MDA values, it was found that the addition of NAC pro-tected the kidney and brain against the oxidative damage induced by irradiation but NAC addition could remain insuffi-cient for the liver.

  • 
  AtıfOpenAlex2

  İncele
• MakaleAçık Erişim2020Scopus SJRQ4/r/munzur/3167
  The Effect of Resveratrol on The Oxidative Stress in Rats Treated with Irradiation

  Ozcelik, Mehtap; Erisir, Mine; Guler, Osman; Baykara, Murat; Bulmus, Funda Gulcu

  The antioxidant and protective effect of resveratrol against irradiation induced oxidative damage were researched. Twenty-one rats were seperated into 3 groups; First group was the control group. Second group was the irradiation group; received physiological saline intraperitoneally (i.p.) for three days, then 9 Gray (Gy) irradiation was applied. Third group was irradiation+Resveratrol group; received 10 mg/kg resveratrol i.p. for three days and then the same dose of radiation was applied. As a result of irradiation, a significant increase was found in the level of malondialdehyde (MDA) in the liver, kidney and brain tissues of rats (P<0.001). Glutathione peroxidase (GSH-Px) activity in all the tissues and superoxide dismutase (SOD) activity, Glutathione (GSH) levels in liver decreased (P<0.01), GSH levels in kidney, spleen and ovarium tissues significantly increased (P<0.05). The giving of resveratrol significantly reduced the MDA levels increasing in liver, kidney, and brain as a result of irradiation (P<0.001) and the liver MDA levels reached to the control levels. By giving resveratrol, it was determined that all the antioxidants studied reduced significantly compared to the control group and/or the irradiation group (P<0.05). According to the data obtained as a result; It was concluded that resveratrol shows a corrective effect by changing the oxidant/antioxidant balance against oxidative stress caused by irradiation, therefore resveratrol is a powerful antioxidant.

  • 
  AtıfScopus2·OpenAlex3

  İncele