Green synthesis of cobalt-oxide nanoparticles with an endemic species Allium tuncelianum and anticancer activity

The green synthesis method used to synthesize Co3O4-NPs has attracted numerous advantages due to its low cost, less time and energy consume, no need of special instruments for experimental and more eco-friendly with environment. In this study, the Co3O4-NPs were synthesized by green synthesis method to overcome the toxicity of chemical synthesis. And Allium tuncelianum plant which is an endemic species in Tunceli was used as a reducing agent and Co(NO3)2.6H2O as a source of cobalt. The synthesized Co3O4-NPs were characterized by using FTIR, XRD, SEM and HR-TEM techniques. The XRD pattern was shown that the Co-NPs were face-centered cubic (fcc) of crystalline nature and the average crystallite size was found nearly 23 nm with a value of COD(R^2): 0.982. The Co3O4-NPs generally had a spherical shape in the SEM images taken at 200 nm. The Co3O4-NPs showed low IC50 value against HeLa cells compare with RPE normal cells.