Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ2Change of micronucleus frequencies, antioxidant parameters and in silico in carp (Cyprinus carpio) fry exposed to ibuprofen
Kirici, Muammer; Tüzün, Burak; Yonar, Muhammet Enis; Poustforoosh, Alireza; Mişe Yonar, Serpil; Şeker, Engin; İspir, Ünal
Ibuprofen, a non-steroidal anti-inflammatory drug, has been detected in the aquatic environment, but little is known about its effects on fish and other aquatic organisms. This study aimed to investigate the effect of ibuprofen on micronucleus levels in erythrocytes and whole-body antioxidant parameters of carp (Cyprinus carpio) fry. Fish were exposed to immersion in two different doses of ibuprofen concentrations (50 and 250 μg/L) for 24, 48, 72 and 96 h and 14 days. No mortalities occurred in any group during the test. Abnormal behaviours such as irregular swimming, loss of balance and hanging vertically in the water were observed in some fish exposed to acute toxicity. In this study, a significant increase in the micronucleus number of red blood cells was observed throughout the study period (p < 0.05). Antioxidant parameters were measured spectrophotometrically. Results obtained showed that ibuprofen significantly increased (p < 0.05) malondialdehyde (MDA) levels, glutathione S-transferase (GST) and catalase (CAT) activities, but decreased (p < 0.05) the glutathione (GSH) levels of whole-body carp fry in a dose-dependent manner. Furthermore, ibuprofen significantly increased MDA, GST and CAT levels, but increased the GSH levels of whole-body carp fry in a time-dependent manner. These results indicate that carp fry is sensible to human pharmaceutical agents in aquatic environments. The manner and the constructed interactions between ibuprofen and two protein targets (immunoglobin and haemoglobin) were investigated using molecular docking and molecular dynamics simulation. The results indicated that ibuprofen could affect the haemoglobin activity. Finally, absorption, distribution, metabolism, excretion and toxicity analysis was performed to examine the drug potential of the studied molecules.
AtıfScopus3OpenAlex5
İncele - MakaleAçık Erişim2026Scopus SJRQ2Cadmium-induced changes in haemato-immunological, oxidative stress and antioxidant parameters of Cyprinus carpio: Protective role of curcumin
Pala, Ayşegül; Eroğlu, Mücahit; Mişe Yonar, Serpil; İspir, Ünal; Yonar, Muhammet Enis
This study examined the adverse effects of sublethal concentrations of cadmium (Cd) and the potential of curcumin (CUR) to mitigate Cd-induced toxicity. We administered 1.5 and 3 mg/L of Cd and 100 mg/kg of CUR to fish (Cyprinus carpio) separately and in combination over 14 days. At the end of the experiments, various haematological and immunological parameters, oxidative stress levels and antioxidant parameters of fish were measured, and the differences between the groups were evaluated. Simultaneous CUR treatment with Cd reversed the changes in haematological parameters in fish, increased immune responses, reduced oxidative stress and normalized the impaired antioxidant enzyme activities. In summary, our study showed that dietary supplementation with CUR may be an effective strategy to reduce the toxic effects of Cd in carp.