Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2025Scopus SJRQ4Protective effect of Momordica charantia against Hepatorenal toxicity induced by Potassium bromate (KBrO3) in rats; [Efecto protector de Momordica charantia contra la toxicidad hepatorrenal inducida por el bromato de potasio (KBrO3) en ratas]
Karagözoğlu, Fatma; Uyar, Ahmet; Akkoyun, Hurrem Turan; Altun, Serdar; Akkoyun, Mahire Bayramoğlu; Aydin, Sevinç; Melek, Sule; Bengü, Aydın Şükrü; Ekin, Suat; Bakir, Ahmet
This study aims to evaluate the effects of Momordica charantia on hepatorenal toxicity caused by potassium bromate (KBrO3) in rats. Wistar rats were divided into 4 groups as control, KBrO3, bitter melon (MC), and KBrO3+MC. Examining the antioxidant enzyme grade of the kidney tissues, it was found that the enzyme activities of catalase (CAT) (P<0.05), glutathione peroxidase (GSH–Px) (P<0.01), and superoxide dismutase (SOD) (P<0.01) decreased in the KBrO3 group in comparison to the control. There was a significant decrease (P<0.001) in glutathione (GSH) levels and an increase (P<0.01) in malondialdehyde (MDA) level in the KBrO3 group in comparison to the control. Examining the antioxidant enzyme activities in liver tissue, it was determined that CAT, GSH–Px, and SOD enzymes reduced significantly (P<0.05, P<0.01, and P<0.001, respectively) in the KBrO3 group in comparison to the control, and the enzyme activity of decreased CAT, GSH–Px, and SOD enzymes significantly elevated (P<0.01) in the MC group. There was a reduction in GSH level in the KBrO3 group in comparison to the control (P<0.01), while an increase was recorded in the KBrO3+MC group (P<0.05). MDA level in liver tissue increased in KBrO3 group in comparison to the control (P<0.01) and MC decreased the MDA level. Histopathological analysis results indicate severe degenerative and necrotic lesions in hepatorenal histoarchitecture of KBrO3 rats in comparison to the control. However, application of MC+KBrO3 significantly reduced the induced hepatorenal injury with a concomitant increase in histopathological lesions. From the immunohistochemical aspect, MC revealed apoptosis concomitant with the suppression of necrosis in the KBrO3-treated rats as demonstrated by the caspase-3 activity.
AtıfOpenAlex1
İncele - MakaleYalnız künye2023Scopus SJRQ2The protective effect of arbutin against potassium bromate-induced oxidative damage in the rat brain
Akkoyun, H. Turan; Uyar, Ahmet; Bayramoglu Akkoyun, Mahire; Bengü, Aydın Şükrü; Melek, Şule; Karagözoğlu, Fatma; Aydın, Sevinç; Ekin, Suat; Erdem, Sinem Aslan
This study aimed to investigate the protective effects of arbutin (ARB) against brain injury induced in rats with potassium bromate (KBrO3). The rats were divided into four groups as Group 1: Control (0.9% NaCl ml/kg/day p.), Group 2: KBrO3 (100 mg/kg (gavage), Group 3: ARB (50 mg/kg/day p.), and Group 4: KBrO3 + ARB (100 mg/kg (gavage) + 50 mg/kg/day p.). At the end of the fifth day of the study, the rats in all groups were killed, and their brain tissues were collected. In the collected brain tissues, malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) levels were measured, and routine histopathological examinations were made. The MDA levels in the group that was exposed to KBrO3 were significantly higher than those in the control group (p ˂ 0.001). In comparison to the KBrO3 group, the MDA levels in the KBrO3 + ARB group were significantly lower (p ˂ 0.001). It was observed that SOD and CAT enzyme activity levels were significantly lower in the KBrO3 group compared to the control group (p ˂ 0.001), while these levels were significantly higher in the KBrO3 + ARB group than in the KBrO3 group (p ˂ 0.001). Additionally, the group that was subjected to KBrO3 toxicity, as well as ARB administration, had much lower levels of histopathologic signs than the group that was subjected to KBrO3 toxicity only. Consequently, it was found that KBrO3 exposure led to injury in the brain tissues of the rats, and using ARB was effective in preventing this injury.
AtıfScopus10
İncele - MakaleAçık Erişim2022Scopus SJRQ3Effects of Arbutin on Fatty Acid Levels of Erythrocyte and Serum in Wistar Albino Rats Treated with Potassium Bromate
Akkoyun, H. Turan; Aydın, Sevinç; Akkoyun, Mahire Bayramoglu; Bengü, Aydın Şükrü; Ekin, Suat; Erdem, Sinem Aslan
Background: In the presented study; the Effects of Arbutin (ARB) on the Rat Erythrocyte and serum fatty acid profile which is exposed to potassium bromate (KBrO3 ) were investigated. Materials and Methods: In this study, 32 Wistar albino rats weighing 250-300 g were used divided into 4 groups. Groups 1: control, group 2: KBrO3 (single dose 100 mg / kg gavage), group 3: ARB (50 mg / kg / day (ip) for 5 days), group 4: KBrO3 + ARB. At the end of the 5th day, alteration of fatty acid profile in erythrocyte and serum of rats in all groups was examined. Results: Rat serum essential fatty acid; palmitic acid (C16:0), myristic acid (C14:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), erythrocyte major fatty acids; palmitic acid (C16:0), myristic acid (C14:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), arachidic acid (C20:0), eicosenoic acid (C20:1), and lignoceric acid (C24:0). In addition, in our studied serum and erythrocytes; Total monounsaturated fatty acids (MUFA) varied between 8.91 ± 0.53-11.71 ± 2.55 and 33.71 ± 2.12-37.11 ± 2.12, respectively. It was determined that total polyunsaturated fatty acids (PUFA) varied between 5.90 ± 1.29-9.96 ± 1.18 and 14.72 ± 3.66-22.13 ± 4.82, respectively. Conclusion: In our study, alterations in fatty acid contents were observed, and results suggesting that arbutine affects the enzymes involved in Fatty acid metabolism and has an effect on fatty acid amounts.
AtıfOpenAlex1İncele - MakaleYalnız künye2017Quercetin'in Koruyucu Etkisinin İncelendiği Ratlarda Alt Ekstremite İskemi-Reperfüzyon Hasarında TAS-TOS Düzeyleri
Akkoyun, Mahire Bayramoğlu; Arihan, Okan; Bengü, Aydın Şükrü; Aydın, Sevinç; Demir, Halit; Birinci, Ceyhun Birinci; Akkoyun, Hurrem Turan; Atçalı, Tuğçe
Objective: It was aimed to determine the protective effectiveness of quercetin in preventing lower extremity ischemia-reperfusion (I/R) injury. Material and Methods: 30 Sprague-Dawley male rats weighing 250-350 g were divided into three groups. Anaesthesia was applied to the first group of rats without ischemia-reperfusion process. To the second group two hours of ischemia and two hours of reperfusion, with the help of left lower extremities tourniquet , were applied. To the third group, 50mg/kg (ip) quercetin application was performed 45 minutes before the ischemia was initiated, two hours of ischemia and two hours of reperfusion application were carried out. At the end of the process, by taking the tissue samples, total antioxidant status (TAS) and total oxidant status (TOS) levels were evaluated. Results:When TAS was evaluated, it was determined that the decrease (p?0.001) occurring in I/R group in comparison with the control group was significant, the decrease (p?0.05) in I/R+Q group in comparison with control group, and again the increase (p?0.001) in I/R+Q group in comparison with I/R group was also significant. When the TOS amounts were evaluated, it was determined that the increase (p?0.001) occurring in I/R group in comparison with control group, the increase in I/R+Q group in comparison with control group (p?0.05), the decrease in I/R+Q group in comparison with I/R group was statistically significant. Conclusion: It has been determined that quercetin has protective effect and decrease the injury occurred in experimental Lower Extremity Ischemia-Reperfusion Injury in Rats.
