Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2016Scopus SJRQ3INFLUENCE OF STOCK DENSITY ON DIGESTIVE ENZYME ACTIVITY (TRYPSIN), HEAT SHOCK PROTEIN 70 (HSP70), AND OXIDATIVE STRESS BIOMARKERS OF NARROW CLAWED CRAYFISH, ASTACUS LEPTODACTYLUS ESCHSCHOLTZ, 1823 (DECAPODA, ASTACIDAE)
Aksu, O.; Kutluyer, F.; Can, E.; Erisir, M.; Benzer, F.
Stocking density causes changes in behaviour and physiology of aquatic animals and high density can cause oxidative damage in living cells. Therefore, experiments were designed to investigate the effects of stocking density on the oxidant-antioxidant response (glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT), and malondialdehyde (MDA)), digestive enzyme activity (trypsin), and heat shock protein 70 (HSP70) of narrow clawed crayfish (Astacus leptodactylus). The experiment was conducted to assess effects by stocking 3, 6, 9 and 12 crayfish/m(2). Crayfish were stocked into 12 fiberglass tanks, three replicates per treatment and fed for 45 days. Our results indicated that activity of GSH-Px, MDA, and GSH levels increased in hepatopancreas (p<0.05) and muscle tissues (p<0.05) with increasing stock concentration while CAT activity decreased (p<0.05). Trypsin and HSP70 insignificantly increased in haemolymph. In conclusion, oxidative stress biomarkers were negatively influenced by increased quantitative changes in stocking density.
AtıfWoS6OpenAlex8
İncele - MakaleYalnız künye2013Scopus SJRQ3POMEGRANATE SEED EXTRACT ATTENUATES CHEMOTHERAPY-INDUCED LIVER DAMAGE IN AN EXPERIMENTAL MODEL OF RABBITS
Yildirim, N. C.; Kandemir, F. M.; Ceribasi, S.; Ozkaraca, M.; Benzer, F.
AtıfOpenAlex14İncele - MakaleYalnız künye2013Scopus SJRQ3Effects of oral administrations of pomegranate seed extract on surgical wound healing in rabbits
Kandemir, F. M.; Sagliyan, A.; Ozkaraca, M.; Gunay, C.; Han, M. C.; Benzer, F.
This study examined the effects of orally given pomegranate seed extract (PSE) on healing of surgically induced full thickness skin wounds in terms of clinical, histopathological and biochemical aspects. A total of 48 healthy, adult male New Zealand rabbits were randomly divided in 2 equal experimental (PSE group) and control groups (C): one week prior the surgery, 100 mg/kg per day via gastric catheter pomegranate seed extract was orally given for 20 days to the assay animals. After one week, all rabitts were anaesthetized by intramuscular injection of xylazine HCl (5 mg/kg) and ketamine HCI (50 mg/kg) and after shaving and disinfection, a circular full thickness skin defect of 2.5 cm diameter was incised on the median line of the back area. Macroscopic and histopathological aspects as well as the tissue oxidant/antioxidant balance were determined on 6 rabbits from each group slaughtered at the 5th, 10th, 15th and 20th days post-surgery.
AtıfWoS9İncele - MakaleYalnız künye2011Scopus SJRQ3EVALUATION OF ENVIRONMENTAL POLLUTION AT MUNZUR RIVER OF TUNCELI APPLYING OXIDATIVE STRESS BIOMARKERS IN CAPOETA TRUTTA (HECKEL, 1843)
Yildirim, N. C.; Benzer, F.; Danabas, D.
Aim of this study was to determine the extent of pollution in the Munzur River in Turkey was determined. The antioxidant (reduced glutathione (GSH), glutathione peroxidase (GSH-Px) catalase (CAT)) and the oxidant (MDA) status of hepatopancreas, gills, muscle, heart and kidney tissues of C. trutta were chosen as bioindicators. The fish samples were caught from contaminated (Station I) and uncontaminated (Station II) stations in Munzur River during April 2010. GSH levels were lower in gill, muscle and heart of the C. trutta collected at the contaminated station (p<0.05). The MDA levels were significantly higher in gill, muscle, kidney and heart of the C. trutta from the contaminated station (p<0.05). The CAT activity was significantly increased in the gill tissue at the contaminated station (p<0.05). GSH-Px activity was significantly decreased (p<0.05) in gill, muscle, kidney and heart tissues samples from C. trutta collected at the contaminated station compared to those collected from the uncontaminated station. Overall, the results demonstrate that alteration in the antioxidant enzymes, glutathione system and induction of lipid peroxidation reflects the presence of pollution, which may cause oxidative stress in the C. trutta from Munzur River. The results provide evidence those enzymatic and non-enzymatic biomarkers of oxidative stress.
AtıfWoS24İncele - MakaleYalnız künye2011Scopus SJRQ3Levels of copper in liver, muscle and gill tissues in Capoeta trutta (Heckel, 1843) from Munzur River, Turkey
Danabas, D.; Benzer, F.; Kaplan, O.; Yildirim, N. C.
Heavy metal pollution of waters is a major environmental problem. Due to their toxicity, long persistence, bioaccumulative and nonbiodegradable properties in the food chain, heavy metals constitute a core group of aquatic pollutants. Capoeta trutta is an economically important fish and the object of significant commercial fishery in the Munzur River. Levels of Cu were measured in samples of the muscle, gill and liver tissues of C. trutta from Munzur River. In liver copper levels were higher at Station I (6.15 mg kg(-1)) polluted by domestic waste than Station II (5.48 mg kg(-1)) (P<0.05). The highest copper level (7.25 mg kg(-1)) was measured in muscle tissue at Station II. There was found no statically differences between stations in gill and muscle (P>0.05). It is suggested that ecological condition of freshwater fish and contamination levels in Munzur River must be regularly monitored.
AtıfWoS7İncele - MakaleYalnız künye2011Scopus SJRQ3Plasma nitric oxide concentrations and erythrocyte arginase activities in lambs with contagious ecthyma
Kandemir, F. M.; Issi, M.; Benzer, F.; Gül, Y.; Basbug, O.; Özdemir, N.
The aim of the present study was to investigate the plasma nitric oxide concentrations and the erythrocyte arginase activities in lambs with contagious ecthyma, which frequently occurs in lambs and goat kids. The study was conducted on 10 Akkaraman, 1-2 month old lambs with contagious ecthyma (the clinical diagnosis was based on the presence of typical cauliflower-like proliferative lesions in lips, commissum labials, nostrils and udders) and on 10 apparently healthy lambs (controls) from the same flock. Plasma NO concentrations and erythrocyte arginase activities were determined on all animals using the Griess method and the thiosemicarbazide diacetylmonoxime urea (TDMU) method, respectively. Compared to the control group, the mean plasma NO concentration was significantly increased in the diseased group while the mean arginase activity was markedly depressed. Abnormally high NO concentrations and low enzyme activities were observed in 9 and 10 diseased lambs, respectively. Additionally, a negative correlation was evidenced between the 2 biochemical parameters. These results show that this viral infection is associated with some perturbations in the arginine metabolism, leading preferentially to the synthesis of NO, which is presumed to exhibit some antiviral properties.
AtıfWoS7İncele - MakaleYalnız künye2011Scopus SJRQ3Effect of Pomegranate Seed Extract on Free Radical Damage and Antioxidant Activity Under Cisplatin-Induced Oxidative Stress Conditions in Rabbit Testes
Benzer, F.; Kandemir, F. M.; Yildirim, N. C.; Ozan, S. T.
The aim of this study is to evaluate the protective effect of pomegranate seed extract on cisplatin-induced oxidative stress in rabbits testes. Rabbits were divided into three groups; (i) being control group, (ii) cisplatin group and (iii) cisplatin + pomegranate seed extract group. The level of malondialdehyde and glutathione, the activity of catalase, glutathione peroxidase, were measured in testes tissue of rabbits. Malondialdehyde levels were increased depending on cisplatin. In the case of cisplatin + pomegranate seed extract, malondialdehyde levels were found to be lower than cisplatin group (p < 0.05). In cisplatin group, catalase, glutathione peroxidase activity and glutathione levels were decreased when compared to control but in cisplatin + pomegranate seed extract group, catalase, glutathione peroxidase activity and glutathione levels were found higher than cisplatin group (p < 0.05). The pomegranate seed extract supplementation may play a protective role or decrease the side effects of cisplatin-induced testes toxicity from cancer medicines.
AtıfWoS5İncele - MakaleYalnız künye2011Scopus SJRQ2Compensatory effects of curcumin on cisplatin-induced toxicity in rabbit testis
Kandemir, F. M.; Benzer, F.; Yildirim, N. C.; Ozdemir, N.
Curcumin, a widely used spice and colouring agent in food, has been shown to possess potent antioxidant, antitumor promoting and anti-inflammatory properties in vitro and in vivo. The present study was designed to investigate the protective effects of curcumin on changes in the levels of lipid peroxidation and endogenous antioxidants induced by cisplatin (cis-diamminedichloroplatinum II, CDDP) in the testiclular tissue of rabbits. 18 healthy male New Zealand white rabbits were equally divided into three groups of six rabbits each, control, cisplatin, and cisplatin+curcumin. The degree of protection produced by cisplatin was evaluated by determining the level of malondialdehyde (MDA) and glutathione (GSH), the activity of catalase (CAT), glutathione peroxidase (GSH-Px), were estimated from testes homogenates. MDA levels were increased with cisplatin compared to control but in cisplatin+curcumin group, MDA levels were found to be lower than cisplatin group (p<0.05). The activity of CAT and GSH-P-X was decreased in cisplatin and cisplatin+curcumin groups compared to control (p<0.05). In the case of cisplatin+curcumin CAT and GSH-Px activity were increased compared to cisplatin group (p<0.05). GSH levels were decreased with cisplatin but administration cisplatin+curcumin increased the levels of GSH comared to cisplatin group (p<0.05). In the present study, co-administration of curcumin with cisplatin prevented the damage to testes induced by this drug and may be considered as a potentially useful candidate in the combination chemotherapy with cisplatin.
AtıfWoS22İncele - MakaleYalnız künye2011Scopus SJRQ3MALONDIALDEHYDE LEVELS AND GLUTATHIONE PEROXIDASE ACTIVITIES IN THE TISSUES OF FRESHWATER CRAYFISH (ASTACUS LEPTODACTYLUS ESCH 1823) IN CULTURE CONDITION
Kandemir, F. M.; Benzer, F.; Erisir, M.; Yildirim, N. C.
The objective of the study was to characterize the effects of sex on lipid peroxidation (as malondialdehyde, MDA), antioxidant defence system (as glutathione peroxidase, GSH-Px) in the hepatopancreas, gonad and muscle tissues of freshwater crayfish, Astacus leptodactylus, in culture condition. The crayfish used in the present study was provided from Keban Dam Lake population of A. leptodactylus. 34 males and 30 females crayfish in different size were hold in stock ponds (6 x 6 x 0.5 m). This study was carried out between June 18, 2004 and December 1, 2004 at the crayfish reproduction unit of Aquaculture Faculty of Firat University, Elazig, Turkey. Plastic pipes (20 cm in length and 7 cm in diameter) were provided as shelters for the crayfish. Crayfish were fed 2 % of their total wet weight daily with prepared ration. Crayfish were exposed to natural photoperiod. During the study, the mean water temperature, dissolved oxygen and mean pH were 4 degrees C, 6.28 +/- 0.32 mg L-1 and 7.82 +/- 0.17, respectively. MDA and GSH-Px levels according to sex and tissue-species were determined. MDA level in the hepatopancreas and gonad of females were higher than males, except muscle tissue (P<0.001). However, the GSH-Px activities in the hepatopancreas and gonad of males were higher than females, except muscle tissue (P<0.001). As a result, MDA and GSH-Px levels in the hepatopancreas, gonad and muscle tissues of freshwater crayfish, Astacus leptodactylus, in culture condition changed both tissues and sex.
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