Sürdürülebilir Kalkınma Amaçları
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- MakaleAçık Erişim2025Scopus SJRQ1Incorporating the HPLC-ESI-Q-TOF-MS profiles with the biochemical properties of eight Salvia species
Llorent-Martínez, Eulogio J.; Nilofar; Sinan, Kouadio Ibrahime; Darendelioglu, Ekrem; Bahsi, Muammer; Polat, Rıdvan; Cakilcioglu, Ugur; Orlando, Giustino; Ferrante, Claudio; Zengin, Gokhan
Salvia, a widely recognized genus globally esteemed for its traditional and medicinal applications, prompted our investigation into the methanol and water extracts of eight Salvia species native to Turkey. These species included S. aucheri, S. candidissima, S. divaricata, S. virgata, S. multicaulis, S. palestina, S. trichoclada, and S. cerino-pruinosa. Our study aimed to assess their biological properties and chemical composition. Utilizing HPLC-ESI-Q-TOF-MS, we identified chemical constituents in the extracts, while antioxidant (radical quenching, reducing power, and metal chelating) and enzyme inhibitory (amylase, glucosidase, tyrosinase, and cholinesterase) activities were evaluated for biological activities. One hundred thirty-one compounds were characterized in the tested extracts. Although many compounds belonging to different chemical families were identified, the main compounds in all the extracts were rosmarinic and sagerinic acids, along with several salvianolic acid isomers. Notably, the methanol extracts displayed superior antioxidant properties overall, with S. cerino-pruinosa exhibiting the highest activity among them, except for metal chelating. Similar to the antioxidant test results, the methanol extracts were more active than the water extracts in the enzyme inhibition tests. Multivariate analysis further highlighted the distinctiveness of the water extract of S. cerino-pruinosa. In conclusion, our findings underscore the biopharmaceutical potential of the tested Salvia species, suggesting their significance as sources of bioactive agents for health-promoting applications.
AtıfScopus17OpenAlex15
İncele - MakaleAçık Erişim2024Scopus SJRQ3Exploring Sorbus torminalis Leaves: Unveiling a Promising Natural Resource for Diverse Chemical and Biological Applications
Ak, Gunes; Tüfekci, Enis Fuat; Mustafa, Ahmed M.; Caprioli, Giovanni; Altunoglu, Yasemin Celik; Baloglu, Mehmet Cengiz; Cakılcıoglu, Ugur; Polat, Rıdvan; Darendelioglu, Ekrem; Zengin, Gokhan
Sorbus torminalis (L.) Crantz has a rich history of versatile applications spanning the fields of medicine and nutrition. It is noteworthy that the decoction obtained from S. torminalis leaves is a traditional treatment method against both diabetes and stomach disorders. Phytochemical profiling determined by HPLC/MS-MS. The effects of the extracts on cell viability were investigated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method against MDA-MB-231 cell line (human breast adenocarcinoma).The ethanol/water extract contained more concentration of total phenolic (91.41 mg gallic acid (GAE) equivalent /gr) and flavanoid (29.10 mg rutin (RE) equivalent/gr) in the tested extract (p<0.05). Resulting of HPLC analysis, the chemical constituents varied depending on the solvents and chlorogenic acid, hyperoside, isoquercetin, delphindin-3,5-diglucoside, procyanidin B2, epicatechin, neochlorogenic acid, 3,5-dicaffeoylquinic acid were identified in all extracts. Overall, ethanol, n-hexane and ethyl acetate extracts showed the highest inhibition for the tyrosinase enzyme. The effect of leaf extracts of S. torminalis on antimicrobial, biofilm inhibitory, and anticancer activities was examined. Based on outcomes of our study recognize this plant as a critical source of medically active chemicals for feasible phytopharmaceutical and nutraceutical applications, providing the first scientific insight into the detailed biological and chemical profiles of S. torminalis.
AtıfScopus7OpenAlex5
İncele - MakaleYalnız künye2023Scopus SJRQ2Chemical Characterization of Different Extracts from Artemisia annua and Their Antioxidant, Enzyme Inhibitory and Anti-Inflammatory Properties
Acquaviva, Alessandra; Nilofar; Bouyahya, Abdelhakim; Zengin, Gokhan; Di Simone, Simonetta Cristina; Recinella, Lucia; Leone, Sheila; Brunetti, Luigi; Uba, Abdullahi Ibrahim; Cakilcioğlu, Ugur
Artemisia annua L. (Asteraceae Family) is an important plant in Asia that has been used for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation. In this study we aimed to evaluate the effects of different polarity extracts (hexane, dichloromethane, ethyl acetate, ethanol, ethanol/water (70 %) and water) from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS. In parallel, chemical composition, antiradical, and enzyme inhibition effects against α-amylase, α-glucosidase, tyrosinase, and cholinesterases were evaluated. The water extract contained the highest content of the total phenolic with 34.59 mg gallic acid equivalent (GAE)/g extract, while the hexane had the highest content of the total flavonoid (20.06 mg rutin equivalent (RE)/g extract). In antioxidant assays, the polar extracts (ethanol, ethanol/water and water) exhibited stronger radical scavenging and reducing power abilities when compared to non-polar extracts. The hexane extract showed the best AChE, tyrosinase and glucosidase inhibitory effects. All extracts revealed effective anti-inflammatory agents, as demonstrated by the blunting effects on COX-2 and TNFα gene expression. These effects seemed to be not related to the only phenolic content. However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
AtıfScopus45OpenAlex43İncele - MakaleYalnız künye2021Scopus SJRQ2Two Medicinal Plants (Alkanna trichophila and Convolvulus galaticus) from Turkey: Chemical Characterization and Biological Perspectives
Mahomoodally, Mohamad Fawzi; Zengin, Gokhan; Sinan, Kouadio Ibrahime; Ak, Gunes; Sadeer, Nabeelah Bibi; Angeloni, Simone; Mustafa, Ahmed M.; Caprioli, Giovanni; Maggi, Filippo; Cakilcioglu, Ugur
The aim of the present study was to quantify selected phenolic compounds, determine antioxidant activity and enzyme inhibitory effects of the aerial parts of Alkanna trichophylla Hub.-Mor. (A. trichophylla) and Convolvulus galaticus Rost.ex Choisy (C. galaticus) extracts prepared by homogenizer-assisted extraction (HAE), maceration (MAC) and infusion techniques. This is the first time such study has been designed to validate the phytochemical composition and bioactivity of these plants. Multivariate analysis was conducted on collected data. Rutin and caffeoylquinic acid derivatives were the most significant compounds in A. trichophylla and C. galaticus, respectively. The highest antioxidant activity of A. trichophylla was mostly exhibited by HAE/methanolic extracts as determined by DPPH, ABTS, FRAP (51.39, 112.70 and 145.73 mg TE/g, respectively) and phosphomolybdenum (2.05 mmol TE/g) assays. However, significant antioxidant activities varied within the extracts of C. galaticus. HAE/methanolic extract of A. trichophylla significantly depressed AChE (2.70 mg GALAE/g), BChE (5.53 mg GALAE/g) and tyrosinase (26.34 mg KAE/g) activities and that of C. galaticus inhibited AChE (2.04 mg GALAE/g), tyrosinase (31.25 mg KAE/g) and α-amylase (0.53 mmol ACAE/g) activities significantly. We concluded that HAE was the most efficient extraction technique as high yield of compounds and promising bioactivities were recorded from extracts prepared. Multivariate analysis showed that types of solvents influenced recovery of compounds and biological activities. This research study can be used as one methodological starting point for further investigation on these plants as all results are clearly promising and open the door to further research challenges such as cytotoxicity evaluation, molecular docking analysis, and more screening of pharmacological actions.
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