Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYayıncıda açık erişim2026Scopus SJRQ2Sperm cryopreservation of sex-reversed rainbow trout (Oncorhynchus mykiss): incorporation of amino acids and anti-oxidants to the extender media to improve the anti-oxidant system
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Benzer, Fulya; Erişir, Mine
Anti-oxidants are vital for protecting sperm and can mitigate the negative effects associated with cryopreservation. This study was conducted to evaluate the protective effects of adding anti-oxidants (vitamins and amino acids) to extender in sex-reversed rainbow trout (Oncorhynchus mykiss) sperm. The collected sperm was diluted at the ratio of 1: 5 by the extenders supplemented with different anti-oxidants including 1.00 mM of ascorbic acid and L-tryptophan, 2.00 mM of cysteine and α-tocopherol, and their combination. After dilution, the semen was aspirated into 0.50 mL straws, and the straws were placed on the tray, frozen for 10 min, and plunged into liquid nitrogen. Straws were thawed in a 30.00 ˚C water bath for 15 sec. The Sperm Class Analyzer System was used to evaluate sperm kinematics. The activity of anti-oxidant enzymes and lipid peroxidation were determined as oxidative stress indices. Our data indicated that the incorporation of anti-oxidants and amino acids increased sperm motility duration. The elevated activity of glutathione peroxidase, superoxide dismutase, and catalase in post-thaw samples indicates that the anti-oxidant system in sex-reversed rainbow trout sperm likely plays a crucial role in protecting membrane compounds from oxidation. In conclusion, the combination of 1.00 mM L-tryptophan and ascorbic acid to the extender media caused a prolonging effect in sperm motility after thawing and they have the potential to serve as effective agents for improving sperm cryosurvival.
- MakaleAçık Erişim2026Scopus SJRQ4INTEGRATED BIOLOGICAL TREATMENT AND ELECTROCOAGULATION FOR SLAUGHTERHOUSE WASTEWATER: A NOVEL APPROACH USING FRESHWATER SNAILS (Viviparus contactus, Planorbis rubrum, Melanoides tuberculata)
Öğedey, Ayşenur; Kocabaş, Filiz Kutluyer; Kizak, Volkan; Kocabaş, Mehmet; Benzer, Fulya; Erişir, Mine; Öğedey, Hayrullah
As a potential pollution source, slaughterhouse wastewater (SWW) with development of the meat production and processing sector is a threat in the world. Therefore, the determination of economical, favourable, cost-effective and proper treatment method is crucial for sustainable environmental protection. This study focused on the performance and efficiency of a two-stage process combining biological treatment (BT) with freshwater snails and electrocoagulation (EC) of SWW. Viviparus contectus, Planorbis rubrum, Melanoides tuberculata were used in primary treatment while secondary treatment was realized with EC process. The EC of SWW was performed for reducing both turbidity and chemical oxygen demand (COD) concentration to levels compliant with direct-discharge regulations. In addition, the stirring speed, current density, initial pH and reaction time were assessed for evaluation the optimum EC condition. Oxidative stress biomarkers and some antioxidants were assessed in freshwater snails exposed to SWW after 24 and 96 h. The best removal efficiencies of COD (91.43%) and turbidity (92.42%) were obtained at pH 5 and 20 min of operational time by P. rubrum. Notably, MDA (Malondialdehyde) was highest level in M. tuberculata compared to V. contectus and P. rubrum. Overall, removing organic and inorganic pollutants from SWW could be provided with the hybrid process BT/EC as a combining approach.
- MakaleAçık Erişim2025Scopus SJRQ3l-Tryptophan improves the sperm quality of threatened Abant trout (Salmo abanticus) against glyphosate toxicity: impact on oxidative stress and DNA damage
Kocabaş, Mehmet; Kocabaş, Filiz Kutluyer; Erişir, Mine; Benzer, Fulya; Arslan, Halil Ozancan; Keleş, Esin; Aksu, Önder
The current study focused on the ameliorating impact of TRP (5 mM) on Salmo abanticus spermatozoa exposed to sub‑lethal concentrations of GLY (2.5, 5, and 10 mg/L). Sperm quality parameters, oxidative stress indices, and DNA damage were analyzed in the post‑exposure sperm. Our data reveal that GLY has an adverse effect on motility rate and duration, sperm kinetics parameters, antioxidant status and DNA damage, and triggers antioxidant defense mechanisms at the highest dose (10 mg/L) and it has insufficient therapeutic effect. Overall, GLY poses potential hazards to male reproductive cells and may adversely affect subsequent generations of natural populations.
- MakaleYalnız künye2025Scopus SJRQ1Naringenin Attenuates Testicular Toxicity and Apoptosis in Rats Chronically Exposed to Mercury Chloride
Kahramanoğullari, Merve; Erişir, Mine; Yaman, Mine; Kaya, Şeyma Özer; Ak, Tuba Parlak
Mercury is an industrial toxin with detrimental effects on the reproductive system. This study explored the impact of naringenin (NAR) on testicular toxicity and apoptosis in rats chronically exposed to mercury chloride (MC). The research involved 41 adult male Wistar-Albino rats aged about 2.5 months, which were allocated into 5 groups: group 1 was a control group, group 2 received 100mg/kg of NAR, group 3 was exposed to MC, group 4 received both MC and 50mg/kg of NAR, and group 5 received both MC and 100mg/kg of NAR. For the treatments, the control group took 1mL/kg saline via intraperitoneal (ip) injection and 1mL/kg corn oil via gavage. MC was administered at 0.4mg/kg/day to the MC groups via ip, and NAR was delivered in corn oil at 50 and 100mg/kg/day to the respective NAR groups via gavage. All treatments were performed over a period of 20 days, with MC administration starting 1 hour after NAR administration. The irregularity and loss in spermatogenic cell organization, degeneration and vacuolization in tubules, edema, and congestion in interstitial areas of the testicular tissue of the MC group were alleviated by NAR administration. In addition, the increase in Caspase 3, iNOS, and eNOS immunoreactivities of the MC group were alleviated by NAR administration. In conclusion, it was found that NAR had protective and therapeutic effects against oxidative damage, histopathological changes, apoptosis, and altered spermatological parameters resulting from MC exposure in the testis, and 100mg/kg NAR provided positive effects on altered histopathological analysis, spermatological parameters, and apoptosis.
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İncele - MakaleAçık Erişim2025Scopus SJRQ3Bioactive Compounds and Antioxidant Properties of Mucus from Freshwater Snails Viviparus contectus and Planorbis rubrum
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Kızak, Volkan; Korkmaz, Büşra; Benzer, Fulya; Erişir, Mine
Abstract: As a viscous-elastic substance, snail mucus has a great attention in the medical and cosmetic industries in recent years due to contain many bioactive compounds. Freshwater snails are cosmopolite aquatic invertebrates and distribute nearly all types of inland aquatic bodies due to their adaptive abilities, rapid growth and short life cycles and, the compounds of mucus derived from freshwater snails has not been well documented. Hence, this study focused on identification bio-active ingredients and determination of protein content and antioxidant enzyme activities in freshwater snails Viviparus contectus and Planorbis rubrum mucus. Bioactive constituents of mucus in freshwater snails were characterized by Gas Chromatography-Mass Spectrometry (GC-FID-MS) analysis. Antioxidants (GSH, SOD, CAT and GSH-Px) and protein content were assessed in freshwater snails. Our data revealed that thirteen compounds are characterized with nonadecanoic acid methyl ester (11.57%), nonadecane (5.01%) and heptadecanoic acid methyl ester (4.19%) as the main components of P. rubrum mucus while eleven compounds are characterized with farnesol (11.23%), 5-hydroxymethyl-1,1,4α-Trimethyl-6-methylene-decahydronphthalen-2-ol (9.73%), kaur-16-en-18-oic acid methyl ester (9.64%), tricosane (9.62%), and kaurene (8.44%) as the main components of V. contectus mucus. Nonadecanoic acid methyl ester and nonadecane, known as effective bioactive constituents having antimutagenic and antitumor capability, were determined with 11.57 and 5.01% of the total bio-active composition of P. rubrum mucus, respectively. As a major constituent, farnesol was detected with 11.23% of the total bio-active composition of V. contectus mucus. Moreover, the presence of kaurene, known as a potent bioactive compound for cancer treatment, in notably quantity in V. contectus mucus. In addition, GSH levels and SOD, CAT, and GPx activities was highest level in P. rubrum compared to V. contectus. Protein content was highest level in V. contectus than P. rubrum. Overall, individual compounds of V. contectus and P. rubrum mucus could be isolated and purified and used for the treatment of a variety of human ailments.
- MakaleAçık Erişim2024Scopus SJRQ2Effects of naringenin on oxidative damage and apoptosis in liver and kidney in rats subjected to chronic mercury chloride
Kahramanoğullari, Merve; Erişir, Mine; Yaman, Mine; Parlak Ak, Tuba
Mercury chloride is a type of heavy metal that causes the formation of free radicals, causing hepatotoxicity, nephrotoxicity and apoptosis. In this study, the effects of naringenin on oxidative stress and apoptosis in the liver and kidney of rats exposed to mercury chloride were investigated. In the study, 41 2-month-old male Wistar-Albino rats were divided into five groups. Accordingly, group 1 was set as control group, group 2 as naringenin-100, group 3 as mercury chloride, group 4 as mercury chloride + naringenin-50, and group 5 as mercury chloride + naringenin-100. For the interventions, 1 mL/kg saline was administered to the control, 0.4 mg/kg/day mercury (II) chloride to the mercury chloride groups by i.p., and 50 and 100 mg/kg/day naringenin prepared in corn oil to the naringenin groups by gavage. All the interventions lasted for 20 days. Mercury chloride administration was initiated 1 h following the administration of naringenin. When mercury chloride and the control group were compared, a significant increase in plasma urea, liver and kidney malondialdehyde (MDA) levels, in kidney superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST) activities (p <.001), and a significant decrease in liver and kidney glutathione (GSH) levels (p <.001), in liver catalase (CAT) activity (p <.01) were observed. In addition, histopathological changes and a significant increase in caspase-3 levels were detected (p <.05). When mercury chloride and treatment groups were compared, the administration of naringenin caused a decrease aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH) (p <.01), urea, creatinine levels (p <.001) in plasma, MDA levels in liver and kidney, SOD, GSH-Px, GST activities in kidney (p <.001), and increased GSH levels in liver and kidney. The addition of naringenin-100 increased GSH levels above the control (p <.001). The administration of naringenin was also decreased histopathological changes and caspase-3 levels (p <.05). Accordingly, it was determined that naringenin is protective and therapeutic against mercury chloride-induced oxidative damage and apoptosis in the liver and kidney, and 100 mg/kg naringenin is more effective in preventing histopathological changes and apoptosis.
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İncele - MakaleAçık Erişim2022Scopus SJRQ3Effects of kefir supplementation on body antioxidant-systems, haemolymph heat-shock protein 70 and trypsin, in the crayfish Astacus leptodactylus Eschscholtz (Decapoda, Astacidea)
Aksu, Önder; Türkoǧlu, Semra; Can, Erkan; Erişir, Mine; Kocabaş, Mehmet; Kayim, Murathan; Benzer, Fulya
This study was designed to investigate the effects of dietary kefir on trypsin, heat shock protein 70 (HSP70) as well as the oxidant and antioxidant status of the freshwater crayfish, Astacus leptodactylus. The experiment was performed with the four following treatments: Control group, D1, D2 and D3 (kefir supplemented diet 2, 4, 8 g/kg of crayfish body mass, respectively). Crayfish were stocked into 12 fibreglass tanks (three replicates per treatment) and fed for 45 days. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and catalase (CAT) in hepatopancreas and muscle were analysed to determine the status of the oxidant/antioxidant balance. In addition, HSP70 and trypsin were measured in haemolymph. Our results indicated that the activity of GSH-Px, MDA and GSH levels decreased in hepatopancreas ( p > 0.05) and muscle tissues ( p < 0.05) with increasing kefir concentration, while CAT activity increased ( p < 0.05). Trypsin and HSP70 insignificantly decreased in haemolymph.
AtıfScopus1WoS1OpenAlex1İncele - MakaleYalnız künye2022Scopus SJRQ1In vivo and in vitro evolution of the effects of cypermethrin on turbot (Scophthalmus maximus, Linnaeus, 1758) spermatozoa
Beken, Atife Tuba; Saka, Şahin; Aydın, İlhan; Fırat, Kürşat; Suzer, Cüneyt; Benzer, Fulya; Erişir, Mine; Özden, Osman; Hekimoğlu, Müge Aliye; Engin, Serhat
Synthetic pyrethroid pesticide is commonly used in agricultural activities in the Black Sea region during reproduction period of turbot. In this sense, in vivo and in vitro studies have shown that cypermethrin (CYP) could be one of the environmental factors affecting decreasing turbot stocks. In this study, effects of in vivo and in vitro administration of CYP, a synthetic pyrethroid, on sperm kinematics motility (MOT), progressive motility (PM), curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN), straight line velocity (STR), amplitude of lateral head (ALH), beat cross frequency (BCF), oxidative stress biomarkers malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and lipid peroxidation (LPO) and also histopathological alterations in gonads were investigated in spermatazoa of turbot (Schopthalmus maximus). Broodstock was supplied from culture origin and used in spawning season, additionally, two (0, 0.187 and 0.218 ppb) and three (0, 1.025, 2.05 and 4.1 ppb) different CYP concentrations were performed for in vivo and in vitro studies, respectively. In vivo and in vitro studies, significant reductions were found in sperm MOT, PM, VCL, VSL, VAP, LIN, and ALH properties depend on the increase in CYP concentrations (p < 0.05). Besides, activities of GSH, GPx, SOD, and CAT increased. In terms of histological alterations, no difference was observed among groups (0, 0.187 and 0.218 ppb) in the maturity stage of the germ cells. According to obtained results, sperm kinematics was affected significantly with increased the dose levels of CYP (p < 0.05).
AtıfScopus6OpenAlex8İncele - MakaleAçık Erişim2022Scopus SJRQ1Aroclor 1254 impairs sperm quality, fertilization ability, and embryo development of rainbow trout (Oncorhynchus mykiss)
Kocabaş, Mehmet; Kocabaş, Filiz Kutluyer; Aksu, Önder; Benzer, Fulya; Erişir, Mine; Sahilli, Yeliz Çakir
The polychlorinated biphenyls (PCBs) in aquatic environment adversely affect non-target organisms, including fish. Especially, the male reproduction and next generation can be damaged through high exposure to these pollutants. Hence, the sperm cells were exposed to sublethal concentrations of Aroclor 1254 (0, 1, 5, 10, or 25 mg/l) for 4 h. The sperm quality parameters were analyzed by SCA (Sperm Class Analyzer). The fertility, eyeing, and hatching rates were determined as gamete markers. Lipid peroxidation (malondialdehyde-MDA), glutathione (GSH), and antioxidant enzymes [superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT)] were measured for determination of oxidative stress. Our results showed that Aroclor 1254 negatively affected the motility rate and duration, fertilization rate, embryogenesis, and hatching and also triggered antioxidant defense mechanisms at the highest concentration (25 mg L−1). Furthermore, linear speed (VSL), linearity index (LIN), and amplitude lateral head (ALH) were significantly changed after exposure to 25 mg L−1, and the lowest concentrations (1 and 10 mg L−1) did not significantly affect the motility and fertilizing capacity. The embryogenesis and hatching were significantly affected by sperm exposure to 1, 10, and 25 mg L−1 of Aroclor 1254. Consequently, Aroclor 1254 causes potential hazards in male germ cells, and the exposure of sperm cells to pollutants can adversely affect next generation of wild populations.
AtıfScopus6WoS5OpenAlex4İncele - MakaleAçık Erişim2019Assessment of Biomarkers of Oxidative Stress and Antioxidant in Wild and Farmed Çoruh trout (Salmo coruhensis) Spermatozoa
Benzer, Fulya; Erişir, Mine; Kocabaş, Mehmet; Kutluyer Kocabaş, Filiz
Behaviour and physiology in fish are affected from environmental factors. Particularly, habitat and culture environment in aquatic animals are essential for life stages. In this study, biomarkers of oxidative stress and antioxidant were compared in wild and farmed Çoruh trout (Salmo coruhensis) spermatozoa. In spermatozoa of Çoruh trout, lipid peroxidation (malondialdehyde), glutathione and enzymatic antioxidant activities (catalase, superoxide dismutase, glutathione peroxidase) were assessed. Our data demonstrated that superoxide dismutase (0.44±1.25 Umg-1 protein), catalase (15.21±4.69 k.g-1 protein), glutathione peroxidase (42.89±0.61 Ug-1 protein), glutathione (0.18±4.01 μmolg-1 cell) and malondialdehyde (2.49±0.12 nmol.g-1 cell) levels in wild fish were low compared to spermatozoa of farmed fish. Overall, alterations in malondialdehyde levels and the antioxidant status could be differences in habitat.
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İncele - MakaleAçık Erişim2019Scopus SJRQ3Effect of the organophosphate insecticide chlorpyrifos exposure on oxidative stress and quality of Salmo coruhensis spermatozoa
Kutluyer, Filiz; Kocabaş, Mehmet; Erişir, Mine; Benzer, Fulya
The use of pesticides has been increasing along with increasing farming activities and has caused deleterious environmental impacts. Non-target organisms in particular, including fish, are affected by pesticides. In this work, the impacts of Chlorpyrifos (CPF) on sperm oxidative stress markers and sperm motility were investigated in vitro. CPF concentrations were 0 μg/L (control), 5 μg/L, 10 μg/L and 15 μg/L. Lipid peroxidation [malondialdehyde (MDA)], nonenzymatic antioxidants [glutathione (GSH)] and enzymatic [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] activities in sperm cells were examined for the determination of oxidative stress status. Our findings showed that motility and survival of sperm cells significantly decreased with exposure to Chlorpyrifos. Biochemical assays revealed that CAT activity and levels of MDA and, GSH increased in spermatozoa based on CPF concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to CPF exposure. It can be deduced that CPF has the potential to disrupt sperm quality and to cause to oxidative stress in sperm cells of S. coruhensis.
AtıfScopus15WoS14OpenAlex13İncele - MakaleAçık Erişim2019Scopus SJRQ3The effect of curcumin on lipid peroxidation and selected antioxidants in irradiated rats
Ozcelik, Mehtap; Erişir, Mine; Guler, Osman; Baykara, Murat; Kirman, Esra
The effect of irradiation on oxidants and antioxidants in selected tissues and a possible protective effect of curcumin on these indices were investigated. A total of 28 rats were divided into 3 groups; group 1 was control; group 2 was the irradiation group, saline was administered intraperiotenally (i.p.) for three days and then, 9 Gy gamma irradiation was applied; group 3 was the irradiation + curcumin group: curcumin was given i.p. for three days at 200 mg/ kg body weight and then the same dose of irradiation was applied. A significant increase in malondialdehyde (MDA) was detected in the liver, kidney, and brain tissues of the rats as a result of irradiation (P ˂ 0.01). Glutathione peroxidase (GSH-Px) activity in all the tissues (except for kidneys) decreased (P ˂ 0.01), liver SOD (superoxide dismutase) activity decreased (P ˂ 0.05), and GSH (glutathione) levels in kidney and ovary tissues (P ˂ 0.001) significantly increased. While curcumin administration returned the increased MDA levels in the kidneys and brain in result of irradiation to normal (P ˂ 0.01), it did not return the increased MDA levels in the liver tissue to normal (P ˂ 0.001) despite significantly reducing them. While decreased GSH-Px and SOD activity in the liver in result of irradiation increased with the addition of curcumin (P ˂ 0.05), increased GSH levels in the kidneys and ovaries returned to control levels (P ˂ 0.001). When MDA values were examined, it was found that the addition of curcumin protected the liver, kidneys and brain from the oxidative damage caused by irradiation.
AtıfScopus8OpenAlex9İncele - MakaleAçık Erişim2019Scopus SJRQ2l-Arginine Improves Sperm Motility in Threatened Coruh Trout Salmo coruhensis (Teleostei: Salmonidae)
Kocabaş, Mehmet; Kutluyer, Filiz; Erişir, Mine; Benzer, Fulya
l-arginine is a versatile amino acid and precursor of nitric oxide that provides protection for preventing free radical damage. Herein, experiments were designed to clarify the effect of l-arginine supplementation on the sperm motility of a threatened trout, Coruh trout Salmo coruhensis. Activation solution was supplemented with levels of 0 mM (Control), 2 mM, 4 mM, and 8 mM l-arginine, and motility and survival of sperm cells were assessed. The selected concentrations were determined according to results of our preliminary experiments. The sperm motility rates at l-arginine concentrations of 0, 2, 4, and 8 mM were 73.33 ± 5.77%, 76.67 ± 5.75%, 80.01 ± 0.01%, and 90.00 ± 0.05%, respectively; notility durations were 21.67 ± 2.08, 32.33 ± 3.79, 32.67 ± 1.15, and 37.00 ± 6.38 s, respectively. Significant effect of l-arginine supplementation was determined on the percentage and duration of motile spermatozoa (p < 0.05). The maximum increment was evoked at the greatest concentration (8 mM). Here, we showed that l-arginine supplement can improve sperm motility of S. coruhensis.
AtıfScopus1WoS1OpenAlex2İncele - MakaleAçık Erişim2018Scopus SJRQ4The Effect of Naringenin on Oxidative Stress in Some Tissues (Heart, Lung, Brain, Spleen, Muscle) of Lead-treated Rats
Benzer, Fulya; Dağ, Üzeyir; Erişir, Mine; Özkaya, Ahmet
Naringenin, a flavonoid increases resistance against oxidative stress. The present study aims to investigate the effect of lead on lipid peroxidasyonu and some antioxidants in heart, lung, brain, spleen, muscle and the effect of naringenin on these parameters. Naringenin was administered by orogastric gavage (50 mg/kg, dissolved in corn oil) and lead acetate was given as daily 500 parts per million in drinking water for 4 weeks. In the rats exposed to lead, MDA levels in the brain, lungs, spleen and muscle tissues significantly increased (P<0.001), in the heart tended to increase but not significantly (P>0.05). Lead caused a statistically significant decrease in GSH-Px activity of the brain, spleen and muscle tissues, in CAT activity of the heart, lung and muscle tissues, in GSH concentration of the lungs and muscle tissues (P<0.05). The administration of alone naringenin caused a statistically significant decrease in MDA levels in the heart and muscle, but a significant increase in the lung (P<0.05). Although increased MDA levels in the heart, lungs, brain, muscle and spleen tissues were significantly decreased by use of the naringenin together with lead (P<0.05), increased MDA levels in the lungs and spleen tissues could not be recovered to the normal level. Generally the decreased GSH-Px and CAT activities in these tissues due to lead were significanly increased by naringenin supplementation (P<0.05). In addition, the reduced GSH levels in lung and muscle, the inceased GSH levels in heart with effect of lead were returned to the normal by naringenin. Lead particularly causes to an increase in lipid peroxidation by consuming antioxidants of cells. Naringenin was able to prevent as tissue-specific (brain, muscle) the oxidative stress caused by lead.
AtıfOpenAlex2TR Dizin2İncele - MakaleYalnız künye2018Scopus SJRQ3Effects of conventional and organic rearing systems and hen age on oxidative stress parameters of blood and ovarian tissues in laying hens
Benzer, Fulya; Baykalır, Yasin; Erişir, Mine; Şimşek, Ülkü Gülcihan
Bu çalışmada yetiştirme sistemleri ve tavuk yaşının, tavukların serum ve yumurtalık dokusundaki bazı oksidatif stres parametreleri üzerine etkileri incelendi. Bu amaçla 30 ve 60 haftalık yaşta, iki farklı sistemden (konvansiyonel kafes ve organik) toplam 48 adet ticari yumurtacı hibrit olan Bovans White kullanıldı. Kesim işlemini takiben tavukların serum ve yumurtalık dokuları toplanarak, malondialdehid (MDA), glutatyon (GSH), glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) düzeyleri belirlendi. Konvansiyonel kafeste yetiştirilen hem 30, hem de 60 haftalık tavukların serum MDA düzeyleri önemli derecede yüksek bulundu (P<0.05). Konvansiyonel kafeste GSH-Px aktivitesi düşük, aksine CAT aktivitesi önemli düzeyde yüksek tespit edildi (P<0.05). Tavuk yaşı serum MDA, GSH, GSH-Px ve CAT düzeylerini etkilemedi (P>0.05). Buna ek olarak, serumda, yetiştirme sistemi ve tavuk yaşı arasında önemli bir etkileşim bulunmadı (P>0.05). Konvansiyonel sistemdeki tavukların yumurtalık dokusunda MDA (P=0.010) ve GSH (P<0.01) seviyeleri önemli düzeyde yüksek bulunurken, GSH seviyesi 60 haftalık yaşta arttı (P<0.01). GSH-Px ve CAT aktiviteleri gruplar arasında benzer çıktı (P>0.05). Yetiştirme sistemi ve tavuk yaşı arasındaki etkileşim yumurtalık dokusunda CAT aktivitesini etkiledi (P<0.05). Sonuç olarak, konvansiyonel bataryalı kafesler, lipid peroksidasyon ve MDA düzeylerinin artmasıyla ilişkili olarak tavuklarda oksidatif strese neden oldu. Bu sistemde MDA'nın azalmasında rol oynayan antioksidan enzimler yetersiz kaldı. Oksidan/antioksidan dengesi 30 ve 60 haftalık yaşlarda tavuk organizması tarafından kurulabildi ve dolayısıyla dokuların MDA düzeyleri yaştan etkilenmedi
AtıfTR Dizin2İncele - MakaleAçık Erişim2018Scopus SJRQ3Cypermethrin-induced in vitro alterations on oxidative stress and quality of salmo coruhensis spermatozoa
Kutluyer, Filiz; Kocabaş, Mehmet; Erişir, Mine; Benzer, Fulya
The use of insecticides has been increasing along with increasing agriculture activities and has caused deleterious environmental impacts. Non-target organisms in particular, including fish, are affected by pesticides. In this work, the impacts of cypermethrin (CYP) on sperm oxidative stress markers and sperm motility were investigated in vitro. The CYP concentrations were 0 μg L-1 control, ethanol), 1.025 μg L-1, 2.05 μg L-1 and 4.1 μg L-1. Lipid peroxidation [Malondialdehyde (MDA)], non-enzymatic antioxidants [glutathione (GSH)] and enzymatic [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] activities in sperm cells were examined for determination of oxidative stress status. Our findings showed that motility and survival of sperm cells significantly decreased with exposure to CYP. Biochemical assays revealed that CAT activity and levels of MDA and, GSH increased in spermatozoa based on CYP concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to CYP exposure. It can be deduced that CYP has the potential to disrupt sperm quality and to cause to oxidative stress in sperm cells of S. coruhensis.
AtıfScopus3WoS3OpenAlex3İncele - MakaleAçık Erişim2018Scopus SJRQ1Malathion-induced spermatozoal oxidative damage and alterations in sperm quality of endangered trout Salmo coruhensis
Kocabaş, Mehmet; Kutluyer, Filiz; Benzer, Fulya; Erişir, Mine
The use of pesticides has been increased along with increasing the farming activities and has caused environmental impacts deleteriously. In particular, non-target organisms including fish can be affected by toxic effects of pesticides. Therefore, the impacts of malathion (MTN) on oxidative stress and sperm quality were investigated in vitro. The MTN concentrations used on this study were 0 (control), 75, 100, and 125 μg/L. Lipid peroxidation (MDA), non-enzymatic (GSH), and enzymatic (SOD, GSH-Px, and CAT) activities in spermatozoa were examined for determination of oxidative stress status. Our findings showed that motility rate and period of sperm cells significantly decreased with exposure to MTN. Biochemical assays revealed that CAT activity and levels of MDA, GSH increased in spermatozoa based on concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to MTN exposure. MTN has disruptive effects on sperm quality and caused to oxidative stress in spermatozoa.
AtıfScopus14WoS14OpenAlex13İncele - MakaleAçık Erişim2018Scopus SJRQ3Effects of conventional and organic rearing systems and hen age on oxidative stress parameters of blood and ovarian tissues in laying hens; [Konvansiyonel ve organik yetiştirme sistemleri ile tavuk yaşının yumurta tavuklarının kan ve yumurtalık dokusundaki oksidatif stres parametreleri üzerine etkisi]
Şimşek, Ülkü Gülcihan; Baykalir, Yasin; Erişir, Mine; Benzer, Fulya
In this study, effects of hen age and rearing systems on some oxidative stress parameters in serum and ovarian tissues of hens are investigated. For this purpose, total of 48 Bovans White commercial laying hybrids from two different rearing systems (conventional cage and organic) at 30 and 60 weeks of age were used. Serum and ovarian tissues of the hens were collected following slaughter process and malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GSH-Px) and catalase (CAT) levels of serum and ovarian tissues were determined. MDA levels of serum was found significantly higher in conventional system, both in 30 and 60 weeks old (P<0.05). GSH-Px activity was lower, conversely CAT activity was significantly higher in conventional system (P<0.05). Hen age didn’t affect MDA, GSH, GSH-Px and CAT levels of serum (P>0.05). In addition, there weren’t any significant interactions between rearing system and hen age in serum (P>0.05). MDA (P=0.010) and GSH (P<0.01) levels of ovarian tissue were found significantly higher in conventional system while GSH levels of ovarian tissue had increased in 60 weeks old (P<0.01). GSH-Px and CAT activity was similar between groups (P>0.05). Interaction between rearing system and hen age affected activity of CAT in ovarian tissue (P<0.05). As a result, conventional battery cages caused oxidative stress interrelated with increasing lipid peroxidation and MDA levels in laying hens. Antioxidant enzymes that play role in reduction of MDA were inadequate in this system. Oxidant/antioxidant balance can be established at 30 or 60 weeks of age by hen organism and therefore MDA levels of tissues would not be affected by age.
AtıfScopus5WoS5OpenAlex5İncele - MakaleYalnız künye2017Ornithobacterium rhinotracheale ile Enfekte Edilen Broylerlerde Lipid Peroksidasyon ve Bazı Antioksidanlar Üzerine Enrofloksasin ve Kafeik Asit Fenetil Ester'in Etkisi *
Benzer, Fulya; Taşdemir, Bülent; Temizer Ozan, Sema; Kılıç, Ayşe; Erişir, Mine; Simsek, Halil; Güler, Osman
Bu araştırmada 4 haftalık 96 broyler civciv 8 gruba bölündü. I. Kontrol Grubu, II. Antibiyotik Grubu, III. Kafeik asit fenetil ester (KAFE) Grubu, IV. Antibiyotik+KAFE Grubu, V.Ornithobacterium rhinotracheale (ORT) Grubu, VI. ORT+Antibiyotik Grubu, VII. ORT+KAFE Grubu ve VIII. ORT+Antibiyotik+KAFE Grubu. ORT 3263/91 suşunu (serotip A) 3.8x108 CFU/mL içeren inokulum aerosol olarak verildi (Grup V-VIII). Gruplara antibiyotik ve KAFE uygulanmasına ORT verilmesinden sonraki 17. günde aynı zamanda başlatıldı. 6 gün süre ile enrofloksasin (10 mg/kg/gün) içme sularına katıldı (Grup II, IV, VI, VIII), KAFE (10 µmol/kg) i.p olarak uygulandı (Grup III, IV, VII, VIII). ORT enfeksiyonu sonucu akciğer dokusunda malondialdehit (MDA) düzeyi (P<0.01) ve superoksid dismutaz (SOD) aktivitesi (P<0.05), trake dokusunda MDA (P<0.001) ve glutatyon (GSH) (P<0.05) düzeyi ile glutatyon peroksidaz (GSH-Px) (P<0.01) aktivitesi arttı, akciğerde GSH-Px (P<0.001) ve katalaz (KAT) aktiviteleri ise (P<0.05) azaldı. Akciğerde kontrol grubuna antibiyotik ve KAFE tek başına ve birlikte uygulanması MDA düzeyinde istatistiki olarak anlamlı artışa (P<0.05), GSH-Px (P<0.01) ve KAT (P<0.001) aktivitelerinde önemli azalmaya sebep oldu. ORT enfeksiyonu sonucu akciğer dokusunda meydana gelen MDA seviyesindeki artış üzerine antibiyotik ve KAFE tek başına ve birlikte önemli azaltıcı etki gösterdi (P<0.001). Trekada kontrol grubuna tek başına antibiyotik ve Ant+KAFE uygulanması sonucu MDA düzeyinde istatistiki olarak anlamlı artış (P<0.001) saptandı. Enfeksiyon grubunda Ant ve KAFE'nin tek başına ve birlikte verilmesinin artan MDA üzerine önemli bir etkisi bulunmadı. Enrofloksasin ve KAFE enfeksiyon durumunda akciğer oksidatif hasarını önlerken treka için etkili bulunmadı. Enrofloksasin ve KAFE kanatlılarda bakterial akciğer hastalıklarında oksidatif hasarı azaltmak için önerilebilir.
- MakaleYalnız künye2014Sığırların normal doğum ve güç doğumunda eritrosit arginaz aktivitesi ve plazma nitrik oksit düzeylerinin karşılaştırılması
Kandemir, Fatih Mehmet; Yüksel, Murat; Benzer, Fulya; Erişir, Mine; Hanedan, Başak
This study was aimed to investigate erythrocyte arginase activity and plasma nitric oxide (NO)levels in dystocia and normal calving of cows and compare these parameters. The study materialconsisted of 16 cows, 3 8 years old, brought for parturition at term to the Clinic of Obstetrics andGynecology at F ı rat University. Blood samples of cows normally calving and dystocia were takeninto blood collecting tubes with heparin from the jugular vein in the first half hour after delivery andcentrifuged at 3000 rpm for 10 minutes. Plasma NO levels and erythrocyte arginase activities weremeasured. The erythrocyte arginase activity in cows normally calving was significantly increasedcompared to that in cows with dystocia (P<0.01), and the plasma NO level in cows normally calvingwas significantly decreased compared to that in cows with dystocia (P<0.05). It was concluded thatthe lower erythrocyte arginase activity and higher plasma NO level in cows might play a role indystocia.