Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYalnız künye2023Scopus SJRQ2Investigation of Antimicrobial, Anti-Quorum Sensing, and Cytotoxic Activities of Flavonoids Isolated from Pulicaria armena Boiss. & Kotschy ex Boiss. (Asteraceae)
Başpınar, Yasemin; Gürbüz, Perihan; Dilem Doğan, Şengül; Gözde Gündüz, Miyase; Aleksic, Ivana; Vojnovic, Sandra; Nikodinovic-Runic, Jasmina; Yavuz Paksoy, Mehmet
This is the first report on the separation and biological assessment of all metabolites derived from Pulicaria armena (Asteraceae) which is an endemic species narrowly distributed in the eastern part of Turkey. The phytochemical analysis of P. armena resulted in the identification of one simple phenolic glucoside together with eight flavon and flavonol derivatives whose chemical structures were elucidated by NMR experiments and by the comparison of the spectral data with the relevant literature. The screening of all molecules for their antimicrobial, anti-quorum sensing, and cytotoxic activities revealed the biological potential of some of the isolated compounds. Additionally, quorum sensing inhibitory activity of quercetagetin 5,7,3’ trimethyl ether was supported by molecular docking studies in the active site of LasR which is the primary regulator of this cell-to-cell communication system in bacteria. Lastly, the critical molecular properties indicating drug-likeness of the compounds isolated from P. armena were predicted. As microbial infections can be a serious problem for cancer patients with compromised immune systems, this comprehensive phytochemical research on P. armena with its anti-quorum sensing and cytotoxic compounds can provide a new approach to the treatment.
AtıfScopus1OpenAlex1
İncele - MakaleAçık Erişim2021Scopus SJRQ2In vitro biological activity of Salvia fruticosa Mill. infusion against amyloid β-peptide-induced toxicity and inhibition of GSK-3β, CK-1δ, and BACE-1 enzymes relevant to Alzheimer's disease
Gürbüz, Perihan; Dokumacı, Alim Hüseyin; Gündüz, Miyase Gözde; Perez, Concepcion; Göger, Fatih; Paksoy, Mehmet Yavuz; Yerer, Mükerrem Betül; Ömür Demirezer, L.
Salvia species have been traditionally used to improve cognition and have been proved to be a potential natural treatment for Alzheimer's disease. Salvia fruticosa Mill. (Turkish sage or Greek sage) demonstrated to have anticholinergic effects in vitro. The aim of this study was to understand the mechanism underlying the neuroprotective effects of S. fruticosa infusion and its representative compound rosmarinic acid, which was detected by LC-DAD-ESI-MS/MS. The protective effects of the S. fruticosa infusion (SFINF) and its major substance rosmarinic acid (RA) on amyloid beta 1–42 -induced cytotoxicity on SH-SY5Y cells together with p-GSK-3β activation were investigated. Their in vitro inhibitory effects against glycogen synthase kinase 3β, β-secretase, and casein kinase 1δ enzymes were also evaluated. The results showed that treatment with the all tested concentrations, SFINF significantly decreased Aβ 1–42-induced cytotoxicity and exhibited promising in vitro glycogen synthase kinase 3β inhibitory activity below 10 µg/mL (IC50 6.52 ± 1.14 µg/mL), in addition to β-secretase inhibition (IC50 86 ± 2.9 µg/mL) and casein kinase 1δ inhibition (IC50 121.57 ± 4.00). The SFINF (100 µg/mL and 250 µg/mL) also activated the expression of p-GSK-3β in amyloid beta 1–42 treated SH-SY5Y cells. The outcomes of this study demonstrated that the S. fruticosa infusion possessed activity to prevent amyloid beta 1–42 -induced neurotoxicity and provided proof that its mechanism may involve regulation of p-GSK-3β protein.
AtıfScopus18OpenAlex17İncele - MakaleAçık Erişim2019Scopus SJRQ3Bioactivity-guided isolation of anti-inflammatory principles from Cistus parviflorus Lam.
Gürbüz, Perihan; Doğan, Şengül Dilem; Çelik, Gizem Nur; Bekci, Hatice; Cumaoğlu, Ahmet; Gündüz, Miyase Gözde; Paksoy, Mehmet Yavuz
Bioactivity-guided fractionation and purification of Cistus parviflorus led to the isolation of two flavan-3-ol derivatives; gallocatechin (1) and epigallocatechin (2) and two flavonoid glycosides; avicularin (3) and trans-tiliroside (4). The structures of the compounds were elucidated on the basis of NMR and ESIMS data. All compounds were isolated from C. parviflorus for the first time. Compounds 2 and exhibited antiinflammatory activity through decreasing the NOS2 and COX2 levels on LPS+IFN-γ treated RAW 264.7 cells. Molecular docking studies of avicularin, the most potent compound, were carried out in the active sites of NOS2 and COX2 to predict the most plausible binding modes and support the experimental data.
AtıfScopus7OpenAlex8TR Dizin2
İncele - MakaleYalnız künye2017Tanacetum zahlbrucknerı (náb.) grıerson' nun sekonder metabolitleri ve antioksidanetkileri*
Paksoy, Mehmet Yavuz; Gürbüz, Perihan; Karatoprak, Gökçe Şeker; Koşar, Müberra; Eroğlu, Duygu; Doğan, Şengül
Bu çalışma kapsamında Asteraceae familyasına dahilendemik bir tür olan Tanacetum zahlbruckneri (Náb.)Grierson bitkisinin toprak üstü kısımlarından hazırlananmetanol ekstresinin DPPH?, ABTS?+süpürücü aktivitesi ve ?-karoten-linoleik asitle oksidasyon yöntemi ile antioksidan aktiviteleri, Folin- Ciocalteuyöntemiyle toplam fenol, flavonit ve flavonol içeriğiincelenmiştir. Metanol ekstresinden sıvı-sıvı partisyonyöntemiyle n-hekzan, dietil eter, diklorometan ve nbutanol ekstreleri hazırlanmış, n-butanol ekstresindençeşitli kromatografik yöntemler kullanılarak, klorojenikasit (1), rutin (2) ve konduritol F (3) izole edilmiştir.Elde edilen bileşiklerin yapıları spektroskopik yöntemler (IR,1H-NMR,13C-NMR, 2D-NMR, IR, MS) kullanılarakaydınlatılmıştır. Bu çalışma ile Konduritol F Tanacetumtürlerinden ilk defa izole edilmiştir. Klorojenik asit verutinin bitkinin metanol ekstresindeki kantitatif tayiniYüksek Basınçlı Sıvı Kromatografisi ile gerçekleştirilmiştir. İzole edilen bileşiklerin antioksidan aktiviteleri ölçülmüş ve klorojenik asitin aktivitesi, rutin vemetanol ekstresinden daha yüksek bulunmuştur.
AtıfTR Dizin1İncele - BildiriYalnız künye2015Scopus SJRQ2Guaianolides from Chrysophthalmum montanum (DC.) Boiss
Gürbüz, Perihan; Dogan, S. Dilem; Pasayeva, Leyla; Dokumaci, Alim Huseyin; Eroglu, Duygu; Paksoy, Mehmet Yavuz; Yerer-Aycan, Mukerrem Betul