Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYalnız künye2016Scopus SJRQ1Pulsed electric fields on phenylalanine ammonia lyase activity of tomato cell culture
Gürsul, Isil; Gueven, Alper; Grohmann, Anne; Knorr, Dietrich
Pulsed electric field (PEF), among numerous stresses, provides a large potential as an external stimulus to enhance secondary metabolite biosynthesis from plant cell cultures. PEF treatment was combined with the sub-culturing technique to increase phenylalanine ammonia lyase (PAL) activity, the key enzyme for secondary metabolite synthesis from tomato cell culture. In reference to the analysis, maximum accumulation of total phenolic (TP) compounds in the cells was determined as a function of stress reaction time (SRT). Also, PEF application increased PAL activity. PEF resulted in an increase in the porosity of the cell membrane, thus decreasing cell vitality. On the other hand, SRT decreased vitality, while increasing TP content. Sub-culturing enhanced the cell structure and improved the cell membrane permeability. PEF treatment potentially offers an alternative to other methods for increasing PAL activity in plant cell cultures. (C) 2016 Elsevier Ltd. All rights reserved.
AtıfWoS20OpenAlex27
İncele - MakaleAçık Erişim2013Scopus SJRQ2Chemical fingerprints of Raki: a traditional distilled alcoholic beverage
Gueven, Alper
Fourteen brands of commercial Raki samples were analysed chemically and compared with two samples produced in a local distillery, according to traditional methods, to determine whether the raw materials ( grape or raisin) and distillation practices result in distinguishable chemical fingerprints in the final product. The samples were analysed with respect to the content of trans- anethol, methyl alcohol, ethyl alcohol, higher alcohols and other volatiles, using gas chromatography. Principal component analysis showed that chemometric analysis could be used to distinguish between traditional and flavoured Raki products. Copyright (C) 2013 The Institute of Brewing & Distilling
AtıfWoS7OpenAlex11İncele - MakaleYalnız künye2011Scopus SJRQ1Isoflavonoid production by soy plant callus suspension culture
Gueven, Alper; Knorr, Dietrich
Production of aglycone type isoflavonoids, Genistein and Daidzein, and p-glycosidic type isoflavonoids, Genistin and Daidzin, by the soy plant callus suspension culture was investigated in a batch system. Genistein and Daidzein concentrations increased during the exponential growth phase of the biomass. Genistin and Daidzin concentrations were not directly related with biomass production. Methyl jasmonate addition to the growth medium (0.1 kg/m(3)) was found to increase isoflavonoid biosynthesis by 5-6 times. Isoflavonoid production in the 7 days old culture increased with pulsed electric field (PEF) application at 1.6 kV, and aglycone forms were influenced to a greater extent. (C) 2010 Elsevier Ltd. All rights reserved.
AtıfWoS45OpenAlex69İncele