Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2014Scopus SJRQ4Antimicrobial Activity of the Methanol Extracts and Essential Oil with the Composition of Endemic Origanum acutidens (Lamiaceae)
Gulec, Azime Kucukgul; Erecevit, Pinar; Yuce, Ebru; Arslan, Ali; Bagci, Eyup; Kirbag, Sevda
The chemical composition of hydro distilled essential oil from Origanum acutidens (Hand. and Mazz.) Ietswaart was collected from Tunceli (Turkey) were analyzed by GC and GC-MS. Essential oil and methanol extract of Origanum acutidens were tested against Yersinia ruckeri (ATCC 29473), Aeromonas hydrophila (ATCC 607920), Lactococcus garviae (ATCC 43921) Staphylococcus aureus (ATCC 29213) fish pathogenic bacterial strains. The analysis of the essential oil of Origanum acutidens showed the presence of 6 components accounting for 93.8 % of the total amount. Calvacrol (61.8 %), p-cymene (15.5 %) and thymol (12.7 %) that possessed antimicrobial activity were found as the major component. The final concentrations of the methanol extract and the essential oil were determined by using disk diffusion assay. Mean inhibition zones of bacterial strains for Origanum acutidens essential oil varied from 28.0 +/- 1.2 and 36.7 +/- 0.7 mm. The inhibition zones of methanol extract (10 mu L and 20 mu L) of Origanum acutidens against bacterial strains were in the range 8.7 +/- 0.7 - 11.0 +/- 0.6 mm and 12.7 +/- 0.67 - 22.7 +/- 1.3 mm, respectively. Our results suggest that both the methanol extract and the essential oil of Origanum acutidens may be valuable as potential antibacterial agents against the major fish pathogens.
AtıfWoS7OpenAlex12
İncele - MakaleAçık Erişim2013Scopus SJRQ2Effect of mixed use of thyme and fennel oils on biochemical properties and electrolytes in rainbow trout as a response to Yersinia ruckeri infection
Gulec, Azime Kucukgul; Danabas, Durali; Ural, Mesut; Seker, Engin; Arslan, Ali; Serdar, Osman
The aim of study was to compare the effects of supplementation of food with herbal oils (Thymus vulgaris and Foeniculum vulgare) on biochemical properties and electrolytes of rainbow trout infected with Yersinia ruckeri. In total, 120 healthy fish (mean weight 84 +/- 1.02 g) were equally divided into four experimental groups. The experimental study was carried out for one week. The first group was control without supplementation and infection, the second group was infected and without oil supplementation, the third group was supplemented with oils (10 ml.100 g(-1) rates) for one week and infected with Y ruckeri and the last group was oil supplemented without infection. Results indicated that fish fed with dietary supplements showed enhanced bactericidal activity, total protein, albumin, cholesterol, triglyceride and bilirubin compared to the control (P < 0.05). As the value of herbal oils was increased in diets, the plasma glucose level decreased. The levels of K, Na, Ca, and Mg increased whereas Cl values decreased, compared to the control. It can be concluded that diet supplementation with herb oils used in this study can increase disease resistance by increasing levels of some biochemical parameters and electrolytes in rainbow trout to Y ruckeri infection.
AtıfWoS41OpenAlex52İncele - MakaleAçık Erişim2013Scopus SJRQ4Bioaccumulation of Copper and Zinc in Freshwater Crayfish (Astacus leptodactylus) Fed with Zebra Mussels (Dreissena polymorpha) Collected from Keban Dam Lake, Turkey
Aksu, Onder; Gulec, Azime Kucukgul; Demir, Veysel; Danabas, Durali; Yildirim, Numan; Ozcelik, Mehtap; Can, Erkan; Kocabas, Mehmet
This study is aimed to determine the bioaccumulation of copper and zinc in hemolymph of freshwater crayfish (Astacus leptodactylus) fed with zebra mussel (Dreissena polymorpha) collected from Keban Dam Lake. This is the first study on bioaccumulation of Cu and Zn in hemolymph of males and females of A. leptodactylus. The average concentrations of Cu and Zn in the hemolymph samples were found out as 0.54 and 35.07 ppm respectively for male and 0.42 and 15.59 ppm respectively for female control freshwater crayfish. The levels of Zn in the hemolymph of both male and female ones among control and diet groups were statistically significant (P < 0.05). The Cu levels among control and diet groups in female ones were statistically significant (P < 0.05) but not in the male ones (P > 0.05). The results of current study revealed that feed treatment with boiling was not effective at the levels of metal bioaccumulation in hemolymph. It was observed that this metal bioaccumulation is more related with sex differences.
AtıfWoS2OpenAlex2İncele - MakaleYalnız künye2012Scopus SJRQ3DETERMINATION OF SAFETY DOSE OF EUCALYPTUS CAMALDULENSIS HYDROSOL ON MIRROR CARP (CYPRINUS CARPIO)
Altinterim, Basar; Gulec, Azime Kucukgul; Aksu, Onder
In this study, the safety dose of Eucalyptus camaldulensis hydrosol on carp (Cyprinus carpio) was investigated. Powdered eucalyptus leaves were mixed with deionized water and then distilled. The hydrosol was added into aquariums at doses of 0.05, 0.10 and 0.15 ml L-1 Temperature, pH, hardness and dissolved oxygen level of the water were recorded before and after hydrosol adding. The control and test experiments were repeated three times. Data obtained from the safety dose tests of E. camaldulensis hydrosol were evaluated using the Probit Analysis Statistical Method. The 5-h LC50 value (95 % confidence limit) of the safe dose of E. camaldulensis hydrosol was estimated to be 0.93 ml L-1(0.03-0.11). It can be seen that 0.05 ml L-1 doses of eucalyptus hydrosol do not create any kind of mental disorder. In the fish exposed to 0.10 and 0.15 ml L-1 doses, some mental disorders (rolling, numbness) and, at the end of 5 h, mortality in all the trial fish have been determined. The number of dead fish was significantly exposed to E. camaldulensis hydrosol (P<0.05).
AtıfWoS6İncele - MakaleYalnız künye2010Scopus SJRQ3An Investigation on Some Haematological and Biochemical Parameters in Capoeta trutta (Heckel 1843) from Munzur River (Tunceli, Turkey)
Danabas, Durali; Yildirim, Nuran Cikcikoglu; Gulec, Azime Kucukgul; Yildirim, Numan; Kaplan, Olcay
Fishes are largly used for the assessment of aquatic environment quality and are accepted as bioindicators of environmental pollution. This study evaluated haematological and biochemical responses of Capoeta trutta captured in Munzur River, Tunceli, Turkey. Blood and biochemical parameters (Sodium (Na), Potassium (K), Calcium (Ca), Phosphorus (P), Chlorine (Cl) and Iron (Fe)) were determined. The values of Red Blood Cell (RBC) Haemoglobin (Hb), Haemotocrit (Ht), Haemoglobin Concentration (MCHC), Platelet Count (PLT), Mean Platelet Volume (MPV), Plateletcrit (PCT), Platelet Distribution Width (PDW) from examined haematological parameters were found as 0.67+/-0.09 10(12) L(-1), 13.5+/-0.90 g dL(-1), 14.78+/-0.19%, 218.40+/-4.29 fL, 194.75+/-15.36 pg 108.52+/-1.09 g dL(-1), 34.12+/-5.89 10(9)L(-1), 11.92+/-1.65 fL, 0.061+/-0.01% and 19.77+/-1.71 fL, respectively. The values of Fe, Cl, P, Ca, K and Na from examined biochemical parameters were determined as 91.75+/-28.27 g dL(-1), 92.00+/-4.02 mmol L(-1), 13.20+/-1.26mg dL(-1), 11.65+/-0.74 mmol L(-1), 1.30+/-0.21 mmol L(-1) and 132.5+/-3.90 mg dL(-1) respectively. Observation of blood parameters allows the most rapid detection of changes in fish after the exposure to xenobiotics. It can conclude that hematological and biochemical parameters could be ranked as possible biomarkers of pollution.
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