Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ3Changes in common carp (Cyprinus carpio) sperm quality and membrane lipid composition related to cryopreservation by Tribulus terrestris; [Alterações na qualidade do esperma e na composição lipídica da membrana da carpa comum (Cyprinus carpio) relacionadas à criopreservação por Tribulus terrestris]
Ölçülü, Abdullatif; Özçiçek, Esin; Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Kızak, Volkan; Yilmaz, Ökkeş
Docosahexaenoic acid (DHA) was the major polyunsaturated fatty acid (PUFA) in the sperm plasma membrane, and the levels of PUFAs decreased in treated groups during the cryopreservation process. The supplementation of TT (Tribulus terrestris) to Cyprinus carpio sperm at 200 µg∙L-1 positively affected the maintenance of motility parameters and FAs levels after the freeze-thaw cycle, and TT can be used as an herbal medicine instead of synthetic pharmaceuticals to improve the productive performance in aquaculture.
- MakaleYayıncıda açık erişim2026Scopus SJRQ2Sperm cryopreservation of sex-reversed rainbow trout (Oncorhynchus mykiss): incorporation of amino acids and anti-oxidants to the extender media to improve the anti-oxidant system
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Benzer, Fulya; Erişir, Mine
Anti-oxidants are vital for protecting sperm and can mitigate the negative effects associated with cryopreservation. This study was conducted to evaluate the protective effects of adding anti-oxidants (vitamins and amino acids) to extender in sex-reversed rainbow trout (Oncorhynchus mykiss) sperm. The collected sperm was diluted at the ratio of 1: 5 by the extenders supplemented with different anti-oxidants including 1.00 mM of ascorbic acid and L-tryptophan, 2.00 mM of cysteine and α-tocopherol, and their combination. After dilution, the semen was aspirated into 0.50 mL straws, and the straws were placed on the tray, frozen for 10 min, and plunged into liquid nitrogen. Straws were thawed in a 30.00 ˚C water bath for 15 sec. The Sperm Class Analyzer System was used to evaluate sperm kinematics. The activity of anti-oxidant enzymes and lipid peroxidation were determined as oxidative stress indices. Our data indicated that the incorporation of anti-oxidants and amino acids increased sperm motility duration. The elevated activity of glutathione peroxidase, superoxide dismutase, and catalase in post-thaw samples indicates that the anti-oxidant system in sex-reversed rainbow trout sperm likely plays a crucial role in protecting membrane compounds from oxidation. In conclusion, the combination of 1.00 mM L-tryptophan and ascorbic acid to the extender media caused a prolonging effect in sperm motility after thawing and they have the potential to serve as effective agents for improving sperm cryosurvival.
- MakaleYalnız künye2026Scopus SJRQ3Characteristics of the growth rate, fatty acid profile, and shell content of the freshwater snail Viviparus contectus (Millet, 1813)
Kocabaş, Filiz Kutluyer; Kizak, Volkan; Özçiçek, Esin; Kocabaş, Mehmet; Yilmaz, Ökkeş
Freshwater snails are a nutritious food source, providing vital amino acids, minerals, and protein with low lipid levels. Hence, the adaptation ability, growth rate, fatty acid (FA) profile, and shell content of Viviparus contectus were determined. Biometric measurements were recorded for 6 months. Over the 6-month cultivation period, specific growth rates were recorded as 0.32% · day-1 for shell length and 0.92% · day-1 for body weight. The FA profile of the freshwater snail included a total of 32 compounds with saturated, monounsaturated, and polyunsaturated FAs represented by 13, 10, and 9 types, respectively. Monounsaturated fatty acids (MUFAs) exhibited the highest concentration, followed by saturated fatty acids (SFAs) s at 35.14% and polyunsaturated fatty acids (PUFAs) at 14.57%. Stearic acid (C18:0), erucic acid (C22:1 n-9), and arachidonic acid (C20:4 n-6) were determined as the most abundant FAs within the saturated, monounsaturated, and polyunsaturated FAs classes, respectively. The energy-dispersive X-ray spectroscopy spectra analysis revealed that the shell powder composition consisted of the elements, such as carbon, oxygen, calcium, iron, manganese, and silicium. Overall, V. contectus showed a clear increase in growth over the cultivation period, and future studies are needed to obtain the best culture conditions and intensive production of the species.
- MakaleAçık Erişim2026Scopus SJRQ4INTEGRATED BIOLOGICAL TREATMENT AND ELECTROCOAGULATION FOR SLAUGHTERHOUSE WASTEWATER: A NOVEL APPROACH USING FRESHWATER SNAILS (Viviparus contactus, Planorbis rubrum, Melanoides tuberculata)
Öğedey, Ayşenur; Kocabaş, Filiz Kutluyer; Kizak, Volkan; Kocabaş, Mehmet; Benzer, Fulya; Erişir, Mine; Öğedey, Hayrullah
As a potential pollution source, slaughterhouse wastewater (SWW) with development of the meat production and processing sector is a threat in the world. Therefore, the determination of economical, favourable, cost-effective and proper treatment method is crucial for sustainable environmental protection. This study focused on the performance and efficiency of a two-stage process combining biological treatment (BT) with freshwater snails and electrocoagulation (EC) of SWW. Viviparus contectus, Planorbis rubrum, Melanoides tuberculata were used in primary treatment while secondary treatment was realized with EC process. The EC of SWW was performed for reducing both turbidity and chemical oxygen demand (COD) concentration to levels compliant with direct-discharge regulations. In addition, the stirring speed, current density, initial pH and reaction time were assessed for evaluation the optimum EC condition. Oxidative stress biomarkers and some antioxidants were assessed in freshwater snails exposed to SWW after 24 and 96 h. The best removal efficiencies of COD (91.43%) and turbidity (92.42%) were obtained at pH 5 and 20 min of operational time by P. rubrum. Notably, MDA (Malondialdehyde) was highest level in M. tuberculata compared to V. contectus and P. rubrum. Overall, removing organic and inorganic pollutants from SWW could be provided with the hybrid process BT/EC as a combining approach.
- MakaleAçık Erişim2025Scopus SJRQ3l-Tryptophan improves the sperm quality of threatened Abant trout (Salmo abanticus) against glyphosate toxicity: impact on oxidative stress and DNA damage
Kocabaş, Mehmet; Kocabaş, Filiz Kutluyer; Erişir, Mine; Benzer, Fulya; Arslan, Halil Ozancan; Keleş, Esin; Aksu, Önder
The current study focused on the ameliorating impact of TRP (5 mM) on Salmo abanticus spermatozoa exposed to sub‑lethal concentrations of GLY (2.5, 5, and 10 mg/L). Sperm quality parameters, oxidative stress indices, and DNA damage were analyzed in the post‑exposure sperm. Our data reveal that GLY has an adverse effect on motility rate and duration, sperm kinetics parameters, antioxidant status and DNA damage, and triggers antioxidant defense mechanisms at the highest dose (10 mg/L) and it has insufficient therapeutic effect. Overall, GLY poses potential hazards to male reproductive cells and may adversely affect subsequent generations of natural populations.
- MakaleYalnız künye2025Scopus SJRQ3New Record of Two Melanopsid Species (Melanopsis costata and Melanopsis buccinoidea) in the Eastern Mediterranean Basin: Morphological Variation in the Söğütlü Stream (Hatay, Türkiye)
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet
Abstract: Melanopsis costata and Melanopsis buccinoidea are freshwater gastropods within the Melanopsidae family characterized by their wide geographical distribution. The geographical distribution of the two species was evaluated within the Eastern Mediterranean Basin as part of this study. Aquatic sites were surveyed across diverse habitats in Söğütlü Stream (Hatay, Türkiye). The shell characteristics [shell height (SH), shell width (SW), spire height (SpH), aperture length (AL), and aperture width (AW)] of the two species were also examined within the ecosystem. The maximum values recorded for M. costata were a shell length (SL) of 17.40 mm and a weight (W) of 0.62 g. The highest values for M. buccinoidea were 13.75 mm for SL and 0.30 g for W. The PCA analysis demonstrated a significant relationship between shell length and shell width, aperture length, aperture width, and weight. The initial evidence of the presence of M. costata and M. buccinoidea was identified in the water body, and the distribution of both species exhibited habitat-dependent variations in their morphological traits.
- MakaleAçık Erişim2025Scopus SJRQ3Bioactive Compounds and Antioxidant Properties of Mucus from Freshwater Snails Viviparus contectus and Planorbis rubrum
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Kızak, Volkan; Korkmaz, Büşra; Benzer, Fulya; Erişir, Mine
Abstract: As a viscous-elastic substance, snail mucus has a great attention in the medical and cosmetic industries in recent years due to contain many bioactive compounds. Freshwater snails are cosmopolite aquatic invertebrates and distribute nearly all types of inland aquatic bodies due to their adaptive abilities, rapid growth and short life cycles and, the compounds of mucus derived from freshwater snails has not been well documented. Hence, this study focused on identification bio-active ingredients and determination of protein content and antioxidant enzyme activities in freshwater snails Viviparus contectus and Planorbis rubrum mucus. Bioactive constituents of mucus in freshwater snails were characterized by Gas Chromatography-Mass Spectrometry (GC-FID-MS) analysis. Antioxidants (GSH, SOD, CAT and GSH-Px) and protein content were assessed in freshwater snails. Our data revealed that thirteen compounds are characterized with nonadecanoic acid methyl ester (11.57%), nonadecane (5.01%) and heptadecanoic acid methyl ester (4.19%) as the main components of P. rubrum mucus while eleven compounds are characterized with farnesol (11.23%), 5-hydroxymethyl-1,1,4α-Trimethyl-6-methylene-decahydronphthalen-2-ol (9.73%), kaur-16-en-18-oic acid methyl ester (9.64%), tricosane (9.62%), and kaurene (8.44%) as the main components of V. contectus mucus. Nonadecanoic acid methyl ester and nonadecane, known as effective bioactive constituents having antimutagenic and antitumor capability, were determined with 11.57 and 5.01% of the total bio-active composition of P. rubrum mucus, respectively. As a major constituent, farnesol was detected with 11.23% of the total bio-active composition of V. contectus mucus. Moreover, the presence of kaurene, known as a potent bioactive compound for cancer treatment, in notably quantity in V. contectus mucus. In addition, GSH levels and SOD, CAT, and GPx activities was highest level in P. rubrum compared to V. contectus. Protein content was highest level in V. contectus than P. rubrum. Overall, individual compounds of V. contectus and P. rubrum mucus could be isolated and purified and used for the treatment of a variety of human ailments.
- MakaleYalnız künye2025Scopus SJRQ3New Record of Peregriana labiata (Rossmässler, 1835) (Gastropoda: Lymnaeidae) in Kelkit Basin (NE Anatolia): Effect of Altitudes on Distribution and Morphological Characters
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Kara, Cemil; Bozali, Nuri; Gürlek, Mustafa Emre
Abstract: Peregriana labiata (Rossmässler, 1835) is a broadly distributed Palaearctic freshwater snail belonging to the family Lymnaeidae and it is an intermediate host of Fascioloides manga causing Fasciolosis as a snail-borne trematode disease. The disease negatively influences the livestock sector and human health with increasing prevalence in the last decades. As a helpful tool, geographical distribution of P. labiata was assessed in Kelkit Basin (North-East Anatolia) as a risk area for fasciolosis transmission. 85 sampling sites were sampled from different habitats in Kelkit Basin (NE Anatolia). The first evidence of P. labiata occurrence was revealed in the basin. In addition, the effects of altitudes on abundance, distribution and shell characters of P. labiata were investigated in several ecosystems. The highest values for SL (standard length) and W (weight) were recorded as 11.80 ± 0.10 mm and 0.17 ± 0.14 g at an altitude of 1233 m, respectively. The highest occurrence of P. labiata was observed at altitudes of 728 m (17%) and 1223 m (16%). PCA analysis showed a strong correlation between SpH (spire height) and AW (aperture width). Overall, the broad range of samples gathered from different altitudes strengthened the idea that the shell characteristics vary with altitude.
- MakaleYalnız künye2025Scopus SJRQ3New Record of Two Dreissenid Species (Dreissena polymorpha gallandi and Dreissena caputlacus) in Kelkit Basin (NE Anatolia): Evaluation of Morphological Divergence Based on Altitude Using Statistical Tools
Kocabaş, Mehmet; Bozali, Nuri; Kocabaş, Filiz Kutluyer; Kara, Cemil; Gürlek, Mustafa Emre
Abstract: Non-indigenous species in an ecosystem increase the potential of novel ecological relationships and can dramatically affect community composition and ecosystem roles. This study examined the spatial distribution of Dreissena caputlacus and Dreissena polymorpha gallandi within the Kelkit Basin located in Northeastern Anatolia. A total of 75 sampling locations representing various habitats were surveyed in the Kelkit Basin. The presence of D. p. gallandi and D. caputlacus was identified for the first time in the basin. The effects of altitude on the population structure, geographic distribution, and shell traits of D. p. gallandi and D. caputlacus were also assessed in a range of ecosystems. The highest shell length (SL) for D. caputlacus was recorded at 566 m altitude with values of 25.58 ± 5.80 mm while the highest SL value for D. p. gallandi was 24.15 ± 5.13 mm at 610 m altitude. Principal Component Analysis (PCA) revealed a strong association among shell length (SL), shell height (SH), shell width (SW), and weight (W). In conclusion, the diverse altitudinal distribution of samples strengthened the evidence that shell features differ with changes in altitude.
- MakaleYalnız künye2025Scopus SJRQ4First Record of Black Sea Trout (Salmo labrax) and Its Potential Hybrids with Endemic Munzur Trout (Salmo munzuricus) in Uzunçayır Dam Lake, Tunceli
Ölçülü, Abdullatif; Düzgüneş, Zehra Duygu; Kocabaş, Filiz Kutluyer; Kızak, Volkan; Kocabaş, Mehmet
The depletion of unique genetic resources and increased risk of extinction may result from hybridization between native and introduced species. In the present study, morphological and molecular analysis were performed on two putative natural interspecific hybrids of the genus Salmo and their parent species, Salmo labrax and Salmo munzuricus, collected from Uzunçayır Dam Lake, which is fed by Munzur and Pülümür streams. Molecular analyses revealed the presence of S. labrax in Uzunçayır Dam Lake for the first time. Phylogenetic analysis using the cytochrome b (Cytb) gene of mitochondrial DNA to determine the maternal origin revealed that hybrid individuals belonged to the same clades as S. labrax or S. munzuricus. Recombination activating gene 1 (Rag1) sequence of nuclear DNA was analyzed for paternal analysis and it was determined that hybrid individuals carried similar single nucleotide polymorphisms (SNPs) to S. labrax or S. munzuricus. These findings support that individuals derive from different parental species. Therefore, evidence from trout samples assumed to be natural hybrids in this study suggests interspecific hybridization between S. labrax and S. munzuricus, but further studies are required. Rag1 Phenotype
- MakaleAçık Erişim2025Scopus SJRQ3Identification and Quantification of Fatty Acid Profile of Muscle and Gametes in Threatened Abant Trout (Salmo abanticus) from Wild
Özçiçek, Esin; Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Yilmaz, Ökkeş
Abstract: As an important challenge restricting hatchery production, gamete quality and subsequent larvae and juvenile are influenced by broodstock nutritional status (i.e., protein, essential amino acids, fatty acid profile and minerals). Hence, the fatty acid (FA) profiles of muscle and gametes in threatened Abant trout (Salmo abanticus) from wild was elucidated and quantitated using the gas chromatography. Our results indicated that the saturated fatty acids (SFAs) in muscle and sperm (38.00 and 60.98%) of S. abanticus occurred in higher quantities than unsaturated fatty acids. The egg of S. abanticus in the wild is characterized by high (mostly n-6 HUFA, 33.59%). Tricosanoic acid (C23:0) and docosahexaenoic acid (DHA, C22:6 n-3) were not determined in sperm of S. abanticus. Overall, the current study provides FA composition in the reproduction cells and muscle of mature S. abanticus and the gametes and muscle had similar general FA profiles with the dominance of C18:1 n-9 (Oleic acid), C16:0 (Palmitic acid), C4:0 (Butyric acid), C18:2 n-6 (Linoleic acid, LA) and C20:5 n-3 (Eicosapentaenoic acid, EPA).
- MakaleYalnız künye2023Scopus SJRQ3Mechanical property and structural-elemental analysis of marine bivalve mollusc shells: Cerastoderma edule, Chamelea gallina, Donax trunculus, Ruditapes decussatus
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Çanakçi, Aykut; Karabacak, A. Hasan
Mussels have great annual production in the world and their shells are discarded as a waste. Shells are calcium and carbon accumulators and, formed as a consequence of calcium carbonate biomineralisation. They can be used as bio‐based composite materials in different fields. Hence, assessment of the chemical compound content and microstructure of shells is important for the prospective utility. For these reasons, structure of Cerastoderma edule, Chamelea gallina, Donax trunculus, Ruditapes decussatus shells were analyzed with X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR) and Scanning Electron Microscope-Energy Dispersive Spectroscopy (SEM-EDS). In addition, microhardness was measured in shells. Our results indicated that shells have a flake like structure with irregular grains appearance morphologically at micrometric scale. The fractions of aragonite in particular shells were assessed. EDS analysis showed that Ca, C and O were major elements of C. edule, C. gallina, D. trunculus, R. decussatus in accordance with XRD data. The lowest microhardness (2.68±0.16 GPa) was in D. trunculus while the highest microhardness (4.28±0.32 GPa) was in C. edule.
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İncele - MakaleYalnız künye2023Scopus SJRQ1Effect of type and concentration of cryoprotectant on the motility, viability, and fertility of climbing perch Anabas testudineus Bloch, 1792 (Pisces: Anabantidae) sperm
Maulida, Siti; Eriani, Kartini; Fadli, Nur; Kocabaş, Filiz Kutluyer; Siti-Azizah, Mohd Nor; Wilkes, Martin; Muchlisin, Zainal Abidin
The climbing perch, Anabas testudineus is a freshwater fish that has economic value in Indonesia. It is cultured in the country, but the breeding technology, specifically sperm storage, is not well developed. Sperm cryopreservation is one of the preservation methods that need to be developed to support fish breeding technology. The type of cryoprotectants and its concentration are species-dependent and determines the success of this approach. Therefore, this study is aimed at determining the optimal type and concentration of cryoprotectant for sperm cryopreservation of A. testudineus. Four separate study series were performed, each of which evaluated one type of cryoprotectant at five concentration levels. The cryoprotectants used were DMSO, methanol, glycerol, and ethanol, and the tested concentrations were 0%, 5%, 10%, 15%, and 20%, which were combined with 5% egg yolks. Each treatment was conducted with three replications. The results showed that the type of cryoprotectant and its concentration significantly affected sperm motility, viability, and fertility of climbing perch (P < 0.05). The best outcome was obtained in DMSO, and methanol at a concentration of 10%, glycerol at 5%, and ethanol at 15%. However, the highest motility, viability, and fertility values were observed at 10% DMSO, indicating it is the best type and concentration for sperm cryopreservation of climbing perch A. testudineus.
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İncele - MakaleAçık Erişim2023Scopus SJRQ4New Approaches to Predict the Sperm Quality by the Spermatozoal miRNA Content in Fish: A Review
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet
The survival of the progeny and fertilization success depend on high-quality mature spermatozoa containing DNA and coding and non-coding RNAs besides the female gamete quality. Sperm cells and gonads in fish contain different RNA classes. It is possible that the development of fish could be affected by miRNA and/or other non-coding RNAs being abundant in Teleost fish and having important functions. Studies about the presence of miRNA in some fish species (e.g. Danio rerio, Salmo salar, Carassius auratus, Cyprinus carpio) showed that sperm motility is regulated by miRNA. Nevertheless, limited knowledge and several hypotheses have been presented about classes and content of RNAs in fish spermatozoa. More research about the effects of miRNA on fish reproduction and sperm quality are needed for population, aquatic ecosystems, and broodstock management, and the evaluation of molecular sperm quality parameters with miRNA could be used together with traditional techniques.
AtıfScopus4İncele - MakaleYalnız künye2022Scopus SJRQ3Ascorbic acid ameliorated the sperm quality of rainbow trout (Oncorhynchus mykiss) against arsenic toxicity: Impact on oxidative stress, fertility ability and embryo development
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Aksu, Önder; Çakir Sahilli, Yeliz
Arsenic (As) is a heavy metal and aquatic pollutant and adversely impacts the reproduction of male fish. As a chain-breaking antioxidant, ascorbic acid (AA) has high water solubility and low toxicity. In this context, the current study was performed to assess the protective role of AA (1 mM) on the sperm cells of the rainbow trout (Oncorhynchus mykiss) exposed to sublethal concentrations of As (8, 16 and 32 mg/L). Sperm quality parameters were analyzed using a sperm class analyzer system. Lipid peroxidation and antioxidant enzyme levels were used as indicators of oxidative stress. The fertilization, eyeing and hatching rates were determined as gamete markers. Reduced sperm quality parameters and fertility capacity resulted from in vitro exposure to As (P < 0.05). The oxidative stress in sperm cells increased after As exposure (P < 0.05). The presence of AA improved sperm movement parameters and fertility potential (P < 0.05). Overall, AA had a positive effect on oxidative stress and fertility ability against As toxicity and AA supplementation ameliorated detrimental effects of As in sperm cells.
AtıfScopus11WoS9OpenAlex5İncele - MakaleAçık Erişim2022Scopus SJRQ1Aroclor 1254 impairs sperm quality, fertilization ability, and embryo development of rainbow trout (Oncorhynchus mykiss)
Kocabaş, Mehmet; Kocabaş, Filiz Kutluyer; Aksu, Önder; Benzer, Fulya; Erişir, Mine; Sahilli, Yeliz Çakir
The polychlorinated biphenyls (PCBs) in aquatic environment adversely affect non-target organisms, including fish. Especially, the male reproduction and next generation can be damaged through high exposure to these pollutants. Hence, the sperm cells were exposed to sublethal concentrations of Aroclor 1254 (0, 1, 5, 10, or 25 mg/l) for 4 h. The sperm quality parameters were analyzed by SCA (Sperm Class Analyzer). The fertility, eyeing, and hatching rates were determined as gamete markers. Lipid peroxidation (malondialdehyde-MDA), glutathione (GSH), and antioxidant enzymes [superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT)] were measured for determination of oxidative stress. Our results showed that Aroclor 1254 negatively affected the motility rate and duration, fertilization rate, embryogenesis, and hatching and also triggered antioxidant defense mechanisms at the highest concentration (25 mg L−1). Furthermore, linear speed (VSL), linearity index (LIN), and amplitude lateral head (ALH) were significantly changed after exposure to 25 mg L−1, and the lowest concentrations (1 and 10 mg L−1) did not significantly affect the motility and fertilizing capacity. The embryogenesis and hatching were significantly affected by sperm exposure to 1, 10, and 25 mg L−1 of Aroclor 1254. Consequently, Aroclor 1254 causes potential hazards in male germ cells, and the exposure of sperm cells to pollutants can adversely affect next generation of wild populations.
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