Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2022Scopus SJRQ2Age- and Sex-Specific Bioaccumulation of Selected Metals in Freshwater Mussel (Unio elangatulus eucirrus Bourguignat, 1860) Populating from Keban Dam Lake (Elazig, Turkey)
Danabaş, Durali; Kutluyer, Filiz; Ural, Mesut; Özçelik, Mehtap; Kocabaş, Mehmet
In aquatic life, environmental chemicals are accumulated by mussels due to their sentinel nature and filter-feeding characteristics. Herein, the present study focused on assessing the concentrations of Cu, Zn, Mg, Mn, Fe, Cd, Pb, Ca, K, and Na levels in freshwater mussels (Unio elangatulus eucirrus) depending on sex and age. For all trace metals, some important differences of bioaccumulations were determined depending on ages and sex. In details, the results indicated that an important age-related accumulation of Mg, Cd, Ca, and K was in females and all trace elements, except Cu, Mg, and K levels in males (p < 0.05). No statistical differences were determined in mean concentrations of Cu, Zn, Mg, and Na. There are statistical differences in Mg, Mn, Fe, and Ca levels between females and males in four aged mussels (p < 0.05). Lead levels were under detectable limits. Overall, metal levels and their toxicity in freshwater mussels should be closely monitored for health of the environment, animals, and humans, since mussels and fish species fed on them are consumed highly in the research region and around.
AtıfScopus5WoS6OpenAlex7
İncele - MakaleYalnız künye2022Scopus SJRQ2Sperm quality and oxidative stress in chub Squalius orientalis and Padanian barbel Barbus plebejus (Teleostei: Cyprinidae) after in vitro exposure to low doses of bisphenol A
Kutluyer, Filiz; Çakir Sahilli, Yeliz; Kocabaş, Mehmet; Aksu, Önder
In an aquatic environment, the presence of Bisphenol A (BPA) adversely affects reproduction, biology, behavior, gonads, and early larval development of fish due to being endocrine-disrupting compound. In addition, the detected concentration of BPA in water bodies is reported to be higher than 0.41 μg/L. As an alternative tool, sperm cells are used in toxicological assays for the reliable and practical assessment. For these reasons, we examined the effects of in vitro exposure of BPA on sperm quality of chub Squalius oriantalis and Padanian barbel Barbus plebejus. Spermatozoa were exposed to lower concentrations (0, 0.5, 1.25, 2.5, and 5 µg/L) of BPA for 2 h. The enzymatic activities [glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD)] and lipid peroxidation (MDA) were evaluated in spermatozoa. The results demonstrated that BPA exposure significantly decreased activities of SOD and GSH-Px but increased CAT activity and lipid peroxidation (MDA). Compared to control, the percentage and duration of sperm motility significantly decreased. Overall, spermatozoa clearly showed the sensitivity to lower concentrations of BPA.
AtıfScopus16WoS17OpenAlex14İncele - MakaleYalnız künye2021Scopus SJRQ2Cellular responses of spirlin Alburnoides bipunctatus spermatozoa exposed to Bisphenol A: Biochemical response and sperm quality alterations evaluation
Kocabaş, Mehmet; Kutluyer, Filiz; Çakir Sahilli, Yeliz; Aksu, Önder
Plastics are one of the global environmental problems owing to their highest production. Bisphenol A (BPA) is used in the manufacturing of plastics including polycarbonate, polyvinyl chloride and resins. In particular, aquatic ecosystems are adversely affected by the occurrence of micropollutants. In addition, BPA as a typical endocrine-disrupting chemical (EDC) seriously influences androgenic activity and reproduction of aquatic organisms, particularly fish as non-target organism. In toxicological assays, sperm cells can be used as a potential tool for reliable and practical assessment. For these reasons, sperm cells were exposed to lower concentrations (0 µg/L, 0.5 µg/L, 1.25 µg/L, 2.5 µg/L and 5 µg/L) of BPA and the study focused on BPA-induced oxidative stress. The enzymatic [glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD)] activities and lipid peroxidation (MDA) were analyzed in spermatozoa. The study findings showed that the activities of SOD and GSH-Px were significantly decreased by BPA but lipid peroxidation (MDA) and CAT acticity increased. Notably, the percentage and duration of motile sperm cells decreased. Collectively, spermatozoa clearly showed the sensitivity even at extremely low concentrations. This is first report to assess the impact of BPA on sperm cells of spirlin Alburnoides bipunctatus.
AtıfScopus6WoS6OpenAlex5İncele - MakaleYalnız künye2021Scopus SJRQ3Glyphosate disrupts sperm quality and induced DNA damage of rainbow trout (Oncorhynchus mykiss) sperm
Akça, Ayşe; Kocabaş, Mehmet; Kutluyer, Filiz
As a widespread pollutant, glyphosate (GLY) adversely affects the aquatic environment and can impair the reproductive ability and functions of fish. The purpose of the current study was to assess in vitro effect of GLY on rainbow trout (Oncorhynchus mykiss) sperm cells. The sperm cells were exposed to different GLY concentrations (2.5, 5, 10 mg/L). Sperm motility parameters were analyzed with computer assisted sperm analysis. DNA fragmentation (%) was measured by the comet assay using fluorescence microscopy. With increased GLY concentration, sperm motility and duration decreased after exposure. DNA fragmentation (% DNA in tail) in sperm cells was higher in treatments containing GLY than control (p < 0.05). Consequently, sperm cells are sensitive to low doses of GLY, and this can negatively affect natural populations.
AtıfScopus8WoS4OpenAlex5İncele - MakaleYalnız künye2020Scopus SJRQ4Spermatologic characteristics and sperm motility alterations caused by short-term copper exposure in Brook Trout Salvelinus fontinalis
Kutluyer, Filiz; Kocabaş, Mehmet; Başçınar, Nadir
Spermatozoa movement in many aquatic animals with external fertilization is directly related to the external aqueous environment. In particular, environmental toxic metals can cause harmful effects and impair sperm cell functions. The high concentrations of copper (Cu) may be harmful for aquatic animals, while low levels are required for metabolic interactions. Herein, we investigated the effect of different copper concentration [0 mg L−1 (Control),.5 mg L−1, 1 mg L–1, and 2 mg L−1] on spermatozoa of brook trout Salvelinus fontinalis. Motility and survival of sperm cells were assessed. Our results indicated that Cu exposure caused to an evident decrease in motility and longevity of spermatozoa with increasing concentrations (p <.05). Overall, we showed that Cu exposure can impair sperm motility of S. fontinalis. Our study suggested that sperm motility has the potential to be a sensitive bioindicator of freshwater environmental pollution.
AtıfScopus3WoS3OpenAlex3İncele - MakaleYalnız künye2020Scopus SJRQ4Metal bioaccumulation in selected tissues of barb (Barbus sp.) and common carp (Cyprinus carpio, Linnaeus 1758) from the Keban Dam Lake, Turkey
Danabas, Durali; Kutluyer, Filiz; Ural, Mesut; Kocabaş, Mehmet
The aquatic life has been negatively influenced by harmful effects of environmental toxic elements, including heavy metals. The elevated concentrations of metals may be harmful for aquatic animals and human health. Herein, the present study deals with assessment of the bioavailability of metals [cadmium (Cd), chrome (Cr), copper (Cu), zinc (Zn), iron (Fe), lead (Pb), manganese (Mn), mercury (Hg), nickel (Ni), and selenium (Se)] in organs such as muscle, gills, liver, kidney, and gonad of the commercially consumed Barbus sp. and Cyprinus carpio (Linnaeus, 1758) collected from Keban Dam Lake, eastern Turkey. The fish were captured from four different stations of Keban Dam Lake. Our results indicated that Fe and Zn concentrations were the highest in all tissues of two fish species while Cd concentrations were the lowest levels. In addition, detected metals accumulated in the muscle of common carp at higher levels compared to Barbus sp. The results from the present study also demonstrated significant variations and considerable differences in the concentrations of all 10 elements between the four stations and different fish tissues. Overall, the concentrations of detected metals in the muscle were many folds higher than the permissible level prescribed by some national and international legislation, which raises health risk concerns.
AtıfScopus11WoS9OpenAlex14İncele - MakaleAçık Erişim2019Scopus SJRQ2Improvement of sperm motility of Oncorhynchus mykiss and Salvelinus fontinalis by L-tryptophan
Kocabaş, Mehmet; Kutluyer, Filiz; Ertekin, Özlem; Aksu, Önder; Başçınar, Nadir
Experiments were designed to test the influence of L-tryptophan supplementation of the extender on the sperm motility parameters and bacterial flora of brook trout (Salvelinus fontinalis) and rainbow trout (Oncorhynchus mykiss). The extender containing 0.3 M glucose in 10% Dimethyl sulfoxide (DMSO) was supplemented with 0, 0.5, 1, 2, and 4 mM L-tryptophan. Sperm samples stored for up to 12 days at 4°C for brook trout were motile while motility was not observed after six days of storage for rainbow trout. Diluted sperm samples were spread-plated onto Plate Count Agar (PCA) (Total Bacteria Count), Rose Bengal Agar (RBC) (Yeast-Mold Count), Violet Red Bile Dextrose (VRBD) (Enterobacteriaceae count) and Mannitol Salt Agar (MSA) (Micrococcus/Staphylococcus count) and counts were performed in duplicate and sampling occurred on day 0, 2, 4, 6, and 12 of cold storage. L-tryptophan supplementation of the extender yielded a positive effect, significantly prolonging sperm motility in S. fontinalis and O. mykiss compared to the control group (p < 0.05). The ideal results were obtained above a concentration of 0.5 mM for both species. Total bacteria count in fresh sperm (undiluted samples) was not detectable and not detected in sperm samples treated with different L-tryptophan concentrations. Yeast-mold, Enterobacteriaceae and Micrococcus/Staphylococcus were not detected from fresh and treated sperm brook trout and rainbow trout. On the basis of the results, L-tryptophan-based extender is useful for maintaining sperm quality in short-term storage. L-tryptophan had a negative effect on the bacterial flora. The results of the current study encourages further studies related to long-term storage and reproduction management. Abbreviations: DMSO: dimethyl sulfoxide; PCA: plate count agar; RBC: rose Bengal agar; VRBD: violet red bile dextrose; MSA: mannitol salt agar; SCA: sperm class analyzer; CFU: colony-forming unit.
AtıfScopus13WoS14OpenAlex13İncele - MakaleAçık Erişim2019Scopus SJRQ3Effect of the organophosphate insecticide chlorpyrifos exposure on oxidative stress and quality of Salmo coruhensis spermatozoa
Kutluyer, Filiz; Kocabaş, Mehmet; Erişir, Mine; Benzer, Fulya
The use of pesticides has been increasing along with increasing farming activities and has caused deleterious environmental impacts. Non-target organisms in particular, including fish, are affected by pesticides. In this work, the impacts of Chlorpyrifos (CPF) on sperm oxidative stress markers and sperm motility were investigated in vitro. CPF concentrations were 0 μg/L (control), 5 μg/L, 10 μg/L and 15 μg/L. Lipid peroxidation [malondialdehyde (MDA)], nonenzymatic antioxidants [glutathione (GSH)] and enzymatic [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] activities in sperm cells were examined for the determination of oxidative stress status. Our findings showed that motility and survival of sperm cells significantly decreased with exposure to Chlorpyrifos. Biochemical assays revealed that CAT activity and levels of MDA and, GSH increased in spermatozoa based on CPF concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to CPF exposure. It can be deduced that CPF has the potential to disrupt sperm quality and to cause to oxidative stress in sperm cells of S. coruhensis.
AtıfScopus15WoS14OpenAlex13İncele - MakaleAçık Erişim2019Scopus SJRQ2l-Arginine Improves Sperm Motility in Threatened Coruh Trout Salmo coruhensis (Teleostei: Salmonidae)
Kocabaş, Mehmet; Kutluyer, Filiz; Erişir, Mine; Benzer, Fulya
l-arginine is a versatile amino acid and precursor of nitric oxide that provides protection for preventing free radical damage. Herein, experiments were designed to clarify the effect of l-arginine supplementation on the sperm motility of a threatened trout, Coruh trout Salmo coruhensis. Activation solution was supplemented with levels of 0 mM (Control), 2 mM, 4 mM, and 8 mM l-arginine, and motility and survival of sperm cells were assessed. The selected concentrations were determined according to results of our preliminary experiments. The sperm motility rates at l-arginine concentrations of 0, 2, 4, and 8 mM were 73.33 ± 5.77%, 76.67 ± 5.75%, 80.01 ± 0.01%, and 90.00 ± 0.05%, respectively; notility durations were 21.67 ± 2.08, 32.33 ± 3.79, 32.67 ± 1.15, and 37.00 ± 6.38 s, respectively. Significant effect of l-arginine supplementation was determined on the percentage and duration of motile spermatozoa (p < 0.05). The maximum increment was evoked at the greatest concentration (8 mM). Here, we showed that l-arginine supplement can improve sperm motility of S. coruhensis.
AtıfScopus1WoS1OpenAlex2İncele - MakaleAçık Erişim2018Scopus SJRQ4Effects of green tea (Camellia sinensis) Oil on Feed Utilization and Hametological Parameters of Rainbow Trout (Oncorhynchus mykiss)
Aksu, Önder; Öztürk, Ekrem; Kutluyer, Filiz; Altınterim, Başar
In the study, experiments were conducted to determine effects of green tea oil (Camellia sinensis) on the growth rate, feed utilization and hematological parameters of rainbow trout (Oncorhynchus mykiss). Fish (76.25±2.75 g) were fed diets supplemented with different concentrations of green tea oil (%0.25, %0.5, %1.0). Weight gain (WG), feed conversion ratio (BDI) and some blood parameters were determined in the rainbow trout. It was determined that the values of WBC, GRAN, HGB, HCT, MCH, MCHC, PLT, PDW and PCT were increased with respect to control group at the end of 42 days feeding with green tea oil. However, groups treated with green tea oil stimulated megakaryocytes (increased PLC-R levels), which also increased platelet production. Feed evaluation ratio varied between 1.83-4.53. At the end of the experiment, it was determined that the feed evaluation ratio decreased with the increase of the amount of green tea oil in the groups. It is thought that the bioactive substances in the lipophilic property of green tea oil provide thermogenic stimulation in fishes by synergistic effect, which causes weight loss. Besides, it was determined that antioxidants such as hematopoiesis and catechins in hematopoietic organs of green tea oil act on the granulocyte cell group.
AtıfOpenAlex10TR Dizin8
İncele - MakaleYalnız künye2018Scopus SJRQ2Phenotypic differentiation analysis: A case study in hybridizing Çoruh trout (Salmo coruhensis), Rize trout (Salmo rizeensis) and brown trout (Salmo trutta fario)
Kocabaş, Mehmet; Kutluyer, Filiz; Başçinar, Nadir
The aim of this study was to determine and compare the phenotypic variation in Çoruh trout (Salmo coruhensis), Rize trout (Salmo rizeensis), brown trout (Salmo trutta fario) and their native hybrids (S. rizeensis × S. t. fario; S. t. fario × S. coruhensis; S. rizeensis × S. coruhensis). The numbers and diameters of red and black spots were determined on operculum, fins and above and below the lateral line. The results from this study indicate that there were differences in spotting pattern, colour pattern or fin pigmentation traits between S. rizeensis, S. t. fario, S. coruhensis and their hybrids. Consequently, native S. rizeensis, S. t. fario and S. coruhensis were affected by interaction and phenotypic plastic aspects of traits in S. rizeensis were dominant in their hybrids (S. rizeensis × S. t. fario; S. rizeensis × S. coruhensis).
AtıfScopus2WoS1OpenAlex3İncele - MakaleAçık Erişim2018Scopus SJRQ3Cypermethrin-induced in vitro alterations on oxidative stress and quality of salmo coruhensis spermatozoa
Kutluyer, Filiz; Kocabaş, Mehmet; Erişir, Mine; Benzer, Fulya
The use of insecticides has been increasing along with increasing agriculture activities and has caused deleterious environmental impacts. Non-target organisms in particular, including fish, are affected by pesticides. In this work, the impacts of cypermethrin (CYP) on sperm oxidative stress markers and sperm motility were investigated in vitro. The CYP concentrations were 0 μg L-1 control, ethanol), 1.025 μg L-1, 2.05 μg L-1 and 4.1 μg L-1. Lipid peroxidation [Malondialdehyde (MDA)], non-enzymatic antioxidants [glutathione (GSH)] and enzymatic [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] activities in sperm cells were examined for determination of oxidative stress status. Our findings showed that motility and survival of sperm cells significantly decreased with exposure to CYP. Biochemical assays revealed that CAT activity and levels of MDA and, GSH increased in spermatozoa based on CYP concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to CYP exposure. It can be deduced that CYP has the potential to disrupt sperm quality and to cause to oxidative stress in sperm cells of S. coruhensis.
AtıfScopus3WoS3OpenAlex3İncele - MakaleAçık Erişim2018Scopus SJRQ1Malathion-induced spermatozoal oxidative damage and alterations in sperm quality of endangered trout Salmo coruhensis
Kocabaş, Mehmet; Kutluyer, Filiz; Benzer, Fulya; Erişir, Mine
The use of pesticides has been increased along with increasing the farming activities and has caused environmental impacts deleteriously. In particular, non-target organisms including fish can be affected by toxic effects of pesticides. Therefore, the impacts of malathion (MTN) on oxidative stress and sperm quality were investigated in vitro. The MTN concentrations used on this study were 0 (control), 75, 100, and 125 μg/L. Lipid peroxidation (MDA), non-enzymatic (GSH), and enzymatic (SOD, GSH-Px, and CAT) activities in spermatozoa were examined for determination of oxidative stress status. Our findings showed that motility rate and period of sperm cells significantly decreased with exposure to MTN. Biochemical assays revealed that CAT activity and levels of MDA, GSH increased in spermatozoa based on concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to MTN exposure. MTN has disruptive effects on sperm quality and caused to oxidative stress in spermatozoa.
AtıfScopus14WoS14OpenAlex13İncele - MakaleAçık Erişim2018Scopus SJRQ4Comparison of oxidant and antioxidant status of Çoruh trout (Salmo coruhensis), anatolian trout (Salmo rizeensis) and rainbow trout (Oncorynchus mykiss) spermatozoa
Kutluyer, Filiz; Kocabaş, Mehmet; Eri̇Şi̇r, Mine; Benzer, Fulya
The aim of present study was to compare oxidant and antioxidant status of Çoruh trout (Salmo coruhensis), Anatolian trout (Salmo rizeensis) and rainbow trout (Oncorynchus mykiss) spermatozoa. Fish were obtained from Uzungöl. Enzymatic antioxidant activities (superoxide dismutase, catalase, glutathione peroxidase), glutathione and lipid peroxidation (malondialdehyde) were determined in spermatozoa of three trout species. Results indicated that catalase (23.36±0.36 K/g.protein), glutathione peroxidase (74.00±1.5 U/g.protein), glutathione (0.57±1.24 µmol/g.cell) and malondialdehyde levels (6.55±2.01 nmol/g cell) were highest levels in Anatolian trout (S. rizeensis) spermatozoa. In conclusion, differences among species caused alterations in the antioxidant and malondialdehyde levels.
AtıfScopus1OpenAlex2TR Dizin1İncele - MakaleAçık Erişim2018Scopus SJRQ3In vitro effect of L-tryptophan on the quality and fertilizing capacity of sperms of endangered species of trouts
Kutluyer, Filiz
The present study was focused on determining the effect of L-tryptophan on quality of sperm and its fertilizing capacity in endangered trout Salmo coruhensis, Anatolian trout Salmo rizeensis and rainbow trout Oncorhynchus mykiss. Different activation media (NaCl, 0.3%; NaHCO3, 1%) were supplemented with L-tryptophan [Control (0), 0.5, 1, 2, 3, 4 and 5 mM] for assessing sperm motility and its duration, fertility and hatching rate of eggs. The results from the present study indicated that addition of L-tryptophan increased the motility rate and duration in O. mykiss, S. rizeensis and S. coruhensis compared to control group. Highest sperm motility rate and its duration, and fertility and hatching rate of eggs of O. mykiss, S. rizeensis and S. coruhensis were 0.5 mM, 5 mM and 2 mM, respectively. To conclude, sperm quality and fertility were positively affected by quantitative changes different concentrations of L-tryptophan. In addition, L-tryptophan can be used in activation medium for O. mykiss, S. rizeensis and S. coruhensis. Copyright 2018 Zoological Society of Pakistan.
AtıfScopus12WoS12OpenAlex10İncele - MakaleAçık Erişim2017Scopus SJRQ2EFFECT OF DIETARY FISH OIL REPLACEMENT WITH PLANT OILS ON GROWTH PERFORMANCE AND GENE EXPRESSION IN JUVENILE RAINBOW TROUT (ONCORHYNCHUS MYKISS)
Kutluyer, Filiz; Sirkecioglu, Ahmet Necdet; Aksakal, Ercument; Aksakal, Feyza Icoglu; Tunç, Abdullah; Günaydin, Esra
An eight-week feeding trial was conducted to evaluate the effects of total (100%) replacement of dietary fish oil with alternative lipid sources in juvenile rainbow trout. Six iso-nitrogenous and iso-lipidic experimental diets were formulated: CO (14%) -cod liver oil; SSO (14%) -safflower seed oil; SBO (14%) -soybean oil; LO (14%) -linseed oil; SBO (7%) + LO (7%) -a blend of soybean oil and linseed oil; and SSO (7%) + LO (7%) -a blend of safflower seed oil and linseed oil. Growth performance [specific growth rate (SGR), weight gain (WG), food conversion ratio (FCR) and survival rate (SR)], growth hormones [growth hormone (GH-I), insulin-like growth factor-I (IGF-I) and insulin-like growth factor-II (IGF-II)], immune transforming growth factor-beta (TGF-beta) and antioxidant [superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) and glutathione S-transferase (GST)] response, and heat shock protein 70 (HSP70) mRNA levels were determined in muscle and liver. Our data indicated that final weight, weight gain, FCR and SGR showed significant difference among the six dietary treatments (P<0.05) while there were no significant differences in survival rate between the rainbow trout from supplement fed groups and control group. HSP70 mRNA level expression in muscle was higher in fish fed SSO (P<0.05) while highest level in liver was obtained from fish fed SBO compared to the other treatments (P<0.05). There were no significant differences among treatments for TGF-beta mRNA expression level in muscle and liver. In conclusion, growth performance and expression levels of growth hormones, antioxidants, HSP70, except TGF-beta were affected by five separate lipid sources. In addition, LO positively increased growth performance of juvenile rainbow trout by means of preventing oxidative stress and HSP70 and, enhanced expression of growth hormone related gene.
AtıfWoS28OpenAlex29İncele - MakaleYalnız künye2017Scopus SJRQ2Effect of Cobalt on Sperm Motility in an Endangered Trout Species, Salmo coruhensis
Kocabaş, Mehmet; Kutluyer, Filiz
Experiments were designed to examine the in vitro effect of cobalt on sperm motility of the endangered trout species (Salmo coruhensis). Sperm samples were diluted in an immobilizing solution, and activated in a motility-activation solution that was supplemented with cobalt at concentrations of 1, 10, 100 and 1000 mg/L. The percentage of motile sperm and duration of motility were determined. Cobalt concentrations of 1–100 mg/L had a positive effect on the percentage of motile sperm and duration of motility compared to the control group, while a concentration of 1000 mg/L resulted in decreases in these parameters (p < 0.05). The percentages of motile sperm at cobalt concentrations of 0, 1, 10, 100 and 1000 mg/L were 83.33% ± 0.25%, 88.33% ± 0.34%, 89.00% ± 0.57%, 90.00% ± 0.87% and 42.50% ± 0.45%, respectively; and the time durations over which the sperm remained motile were 72.00 ± 0.63, 74.83 ± 0.28, 77.40 ± 0.47, 81.14 ± 0.78, and 50.25 ± 0.67 s, respectively. This study has shown that sperm motility and duration were significantly enhanced (p < 0.05) at cobalt concentrations of 1, 10 and 100 mg/L, relative to controls, and significantly decreased at 1000 mg/L.
AtıfScopus11WoS9OpenAlex14İncele - MakaleYalnız künye2016Scopus SJRQ3CRYOPRESERVATION OF GOLDFISH (Carassius auratus) SPERMATOZOA: EFFECTS OF EXTENDER SUPPLEMENTED WITH TAURINE ON SPERM MOTILITY AND DNA DAMAGE
Kutluyer, Filiz; Ögretmen, Fatih; Inanan, Burak Evren
BACKGROUND: Amino acids, present in seminal plasma at high concentration, protect spermatozoa against cell damage during cryopreservation. OBJECTIVE: Experiments were designed to analyze the effect of semen extender supplemented with taurine on post-thawed sperm motility and duration, as well as DNA damage. MATERIALS AND METHODS: Extenders were supplemented with 1, 2 or 4 mM taurine. Semen samples were diluted at the ratio of 1:9 with the extenders. Diluted samples were aspirated into 0.25 ml French straws and 0.1 ml pellets. DNA damage was assessed with the comet assay after cryopreservation. RESULTS: The percentage and duration of sperm motility were significantly increased by taurine. Additionally, sperm motility and the motility period in pellets were higher than in straws. The best concentration of taurine was 4 mM, and the highest post-thaw motility rate (72.50 +/- 3.54%) and duration (17.50 +/- 0.71 s) were obtained from the extender with 4 mM in pellets. DNA damage was decreased after taurine supplementation. CONCLUSION: Pellets could be used for goldfish sperm cryopreservation. The addition of 4 mM taurine increases the post-thaw motility and decreases DNA damage on goldfish semen.
AtıfWoS11İncele - MakaleAçık Erişim2016Scopus SJRQ1The in vitro effect of cypermethrin on quality and oxidative stress indices of rainbow trout Oncorhynchus mykiss spermatozoa
Kutluyer, Filiz; Benzer, Fulya; Erisir, Mine; Ögretmen, Fatih; Inanan, Burak Evren
There is limited information on the scientific literature about the effect of in vitro exposure of fish sperm to pesticides. In vitro effect of cypermethrin on sperm quality and oxidative stress has not yet been fully investigated. Therefore, the effects of cypermethrin, a type II pyrethroid insecticide, on quality and oxidative stress of spermatozoa were examined in vitro. To explore the potential in vitro toxicity of cypermethrin, fish spermatozoa were incubated with different concentrations of cypermethrin (1.025, 2.05 and 4.1 mu g/l) for 2 h. The motility rate and duration of sperm were determined after exposure to cypermethrin. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and malondialdehyde (MDA) in spermatozoa were analyzed for determination of oxidant and antioxidant balance. Our results indicated that spermatozoa motility and duration significantly decreased with exposure to cypermethrin. Additionally, activity of GSH-Px (P < 0.05) and MDA and GSH levels increased in a concentration-dependent manner while CAT activity decreased (P < 0.05). Consequently, the oxidant and antioxidant status and sperm quality were affected by quantitative changes and different concentrations of cypermethrin. (C) 2015 Elsevier Inc. All rights reserved.
AtıfWoS35OpenAlex38İncele - MakaleYalnız künye2015Scopus SJRQ3EFFECTS OF SEMEN EXTENDER SUPPLEMENTED WITH L-METHIONINE AND PACKAGING METHODS (STRAWS AND PELLETS) ON POST-THAW GOLDFISH (Carassius auratus) SPERM QUALITY AND DNA DAMAGE
Kutluyer, Filiz; Ögretmen, Fatih; Inanan, Burak Evren
BACKGROUND: Amino acids protect spermatozoa against cell damage during cryopreservation due to have antioxidant property and found in seminal plasma at high concentration. OBJECTIVE: The aim of the present work was to analyse the effect of extender supplementation with L-methionine on post-thawed sperm motility, duration and DNA damage and also it was tested the feasibility of using straws and pellets method for the cryopreservation of goldfish (Carassius auratus) sperm. MATERIALS AND METHODS: Extenders were supplemented with different L-methionine concentrations of 1 mM; 1.5 mM; 3 mM; 6 mM. Semen samples diluted at the ratio of 1:9 by the extenders were subjected to cryopreservation. After dilution the semen was aspirated into 0.25 ml straws and 0.1 ml pellets, the straws and pellets were placed on the tray, frozen in nitrogen vapor and plunged into liquid nitrogen. DNA damage was determined with comet assay after cryopreservation.. RESULTS: Our results indicated that an increase in the concentration of L-methionine caused a significant increase in the motility rate and duration of sperm in goldfish (C. auratus) (p<0.05). In addition, duration and percentage of motility in pellets were higher than in straws. Comparing all concentrations of L-methionine, the best concentration of L-methionine was 1.5 mM. Highest post-thaw motility (45.00 +/- 7.07%) and duration of motility (17.00 +/- 0.71s) were obtained with the extender at concentration 1.5 mM in pellets. Addition of the extender with L-methionine was reduced DNA damage compared to control group. CONCLUSION: Consequently, pellets could be use for goldfish sperm cryopreservation and the tested amino acid affected the motility parameters, and semen extenders could be supplement with L-methionine.
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