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- MakaleAçık Erişim2026Scopus SJRQ2Change of micronucleus frequencies, antioxidant parameters and in silico in carp (Cyprinus carpio) fry exposed to ibuprofen
Kirici, Muammer; Tüzün, Burak; Yonar, Muhammet Enis; Poustforoosh, Alireza; Mişe Yonar, Serpil; Şeker, Engin; İspir, Ünal
Ibuprofen, a non-steroidal anti-inflammatory drug, has been detected in the aquatic environment, but little is known about its effects on fish and other aquatic organisms. This study aimed to investigate the effect of ibuprofen on micronucleus levels in erythrocytes and whole-body antioxidant parameters of carp (Cyprinus carpio) fry. Fish were exposed to immersion in two different doses of ibuprofen concentrations (50 and 250 μg/L) for 24, 48, 72 and 96 h and 14 days. No mortalities occurred in any group during the test. Abnormal behaviours such as irregular swimming, loss of balance and hanging vertically in the water were observed in some fish exposed to acute toxicity. In this study, a significant increase in the micronucleus number of red blood cells was observed throughout the study period (p < 0.05). Antioxidant parameters were measured spectrophotometrically. Results obtained showed that ibuprofen significantly increased (p < 0.05) malondialdehyde (MDA) levels, glutathione S-transferase (GST) and catalase (CAT) activities, but decreased (p < 0.05) the glutathione (GSH) levels of whole-body carp fry in a dose-dependent manner. Furthermore, ibuprofen significantly increased MDA, GST and CAT levels, but increased the GSH levels of whole-body carp fry in a time-dependent manner. These results indicate that carp fry is sensible to human pharmaceutical agents in aquatic environments. The manner and the constructed interactions between ibuprofen and two protein targets (immunoglobin and haemoglobin) were investigated using molecular docking and molecular dynamics simulation. The results indicated that ibuprofen could affect the haemoglobin activity. Finally, absorption, distribution, metabolism, excretion and toxicity analysis was performed to examine the drug potential of the studied molecules.
AtıfScopus3OpenAlex5
İncele - MakaleAçık Erişim2026Scopus SJRQ2Cadmium-induced changes in haemato-immunological, oxidative stress and antioxidant parameters of Cyprinus carpio: Protective role of curcumin
Pala, Ayşegül; Eroğlu, Mücahit; Mişe Yonar, Serpil; İspir, Ünal; Yonar, Muhammet Enis
This study examined the adverse effects of sublethal concentrations of cadmium (Cd) and the potential of curcumin (CUR) to mitigate Cd-induced toxicity. We administered 1.5 and 3 mg/L of Cd and 100 mg/kg of CUR to fish (Cyprinus carpio) separately and in combination over 14 days. At the end of the experiments, various haematological and immunological parameters, oxidative stress levels and antioxidant parameters of fish were measured, and the differences between the groups were evaluated. Simultaneous CUR treatment with Cd reversed the changes in haematological parameters in fish, increased immune responses, reduced oxidative stress and normalized the impaired antioxidant enzyme activities. In summary, our study showed that dietary supplementation with CUR may be an effective strategy to reduce the toxic effects of Cd in carp.
- MakaleAçık Erişim2022Scopus SJRQ2Effect of chlorpyrifos on some biochemical changes in Cyprinus carpio: the protective effect of ellagic acid
Mişe Yonar, Serpil; Yonar, Muhammet Enis; Ural, Mevlüt Şener; Pala, Ayşegül
In this study, ameliorative effects of ellagic acid (EA) on oxidative stress induced by chlorpyrifos (CPF) in carp, Cyprinus carpio, were investigated. Fish were divided into six groups: C (no treatment), EA (100 mg kg fish−1), CPF-1 (0.040 mg L−1), CPF-2 (0.080 mg L−1), CPF-1 + EA, and CPF-2 + EA. CPF and EA were applied simultaneously for 14 days and, at the end of the study, liver, kidney, and gill samples were collected from fish. On the taken tissue samples, malondialdehyde (MDA) level and some antioxidant enzyme (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione-S-transferase (GST)) activities were evaluated. The results demonstrated statistically significant increases in the MDA levels of the CPF-1 and CPF-2 groups. On the other hand, the MDA levels were significantly decreased by EA administration. Also, CPF exposure caused statistically significant increases in the SOD and GST activities and statistically significant decreases in the CAT and GPx activities. However, treatment with EA reversed negative alterations in the SOD, CAT, GPx, and GST activities. Therefore, the results of this study results showed that simultaneous treatment with EA alleviates CPF-induced oxidative stress in fish.
AtıfScopus8WoS10OpenAlex10İncele - MakaleAçık Erişim2021Scopus SJRQ1Ameliorative effect of Fennel (Foeniculum vulgare) essential oil on chlorpyrifos toxicity in Cyprinus carpio
Pala, Ayşegül; Serdar, Osman; Mişe Yonar, Serpil; Yonar, Muhammet Enis
Chlorpyrifos (CPF) is an organophosphate pesticide that is frequently and widely used to control both agricultural and domestic pests worldwide. In this study, the protective effect of Fennel (Foeniculum vulgare) essential oil (FEO) was investigated in carp (Cyprinus carpio) exposed to CPF. The fish were divided into six groups that one control group (no treatment) and five experimental groups (FEO (3ml/100g diet) group, CPF1 (0.023 mg/l) group CPF2 (0.046 mg/l) group, CPF1 (0.023 mg/l) plus FEO (3ml/100g diet) group, CPF2 (0.046 mg/l) plus FEO (3ml/100g diet) group). Blood and tissue (liver, kidney, gill, and brain) samples were taken from the fish at the end of 14 days of application. Hemoglobin (Hb) level, nitoblue tetrazolium (NBT) activity, and total immunoglobulin (TI) level were measured in blood samples of fish. Acetylcholinesterase (AChE) activity was determined in brain tissue while malondialdehyde (MDA) level, reduced glutathione (GSH) level, catalase (CAT), and glutathione peroxidase (GPx) activity were determined in liver, kidney, and gill tissues. The results showed that there was a significant decrease in Hb level, NBT activity, and TI levels in CPF-treated fish compared to the control group. In addition, increased in MDA levels and significant decreases in GSH level, AChE, CAT, and GPx activities were observed in CPF-treated groups. However, FEO-treated was showed a significant improvement in all parameters except AChE activity compared to CPF groups. These study findings showed that FEO could improve CPF-induced toxicity in C. carpio, except inhibition of AChE activity.
AtıfScopus6WoS6OpenAlex8İncele - MakaleAçık Erişim2020Scopus SJRQ1Effect of trichlorfon on some haematological and biochemical changes in Cyprinus carpio: The ameliorative effect of lycopene
Mişe Yonar, Serpil; Yonar, M. Enis; Pala, Ayşegül; Sağlam, Naim; Sakin, Fatih
Trichlorfon (TCF) is an antiparasitic agent used in aquaculture to treat external parasites and it belongs to the chemical class of organophosphates. This study was conducted to investigate the ameliorating effects of lycopene on TCF-induced toxicity in carp by evaluating haematological parameters and oxidant/antioxidant status. Fish were randomly divided into seven experimental groups. These groups were arranged as follows: control (no treatment) group, corn oil group, lycopene (10 mg kg−1) group, TCF-I (11 mg L−1) group, TCF-I (11 mg L−1) plus lycopene (10 mg kg−1) group, TCF-II (22 mg L−1) group and TCF-II (22 mg L−1) plus lycopene (10 mg kg−1) group. At the end of 14 days administration, blood and tissue (liver, kidney, and gill) samples were collected. Haematological changes [red blood cell (RBC) and white blood cell (WBC) count, haemoglobin (Hb) concentration, haematocrit (Ht) level, and erythrocyte indices: mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC)] were determined in the blood samples, while antioxidant parameters [(malondialdehyde (MDA) and reduced glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities] were evaluated in the liver, kidney and gill samples. The results demonstrated that all the haematological parameters were significantly decreased in the TCF-I and TCF-II groups when compared to the control group. Notable increases in the tissue MDA levels were observed in the TCF-treated groups. In contrast, significant decreases were observed in the tissue SOD, CAT and GSH-Px activities and GSH levels in the TCF-I and TCF-II groups. However, simultaneous treatment with lycopene provided a marked normalisation of the haematological and oxidant/antioxidant parameters compared with the TCF-I and TCF-II groups. The findings show that dietary lycopene supplementation might be helpful in abrogation of TCF toxicity by changing haematological conditions and antioxidant status in fish.
AtıfScopus34WoS28OpenAlex31İncele