Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2019/r/munzur/3752
  Ghanaian Mangrove Wetland Endophytic Fungus, Penicillium herquei strain BRS2A-AR produces (9Z, 11E)-13-oxooctadeca-9,11-dienoic acid with activity against Trichomonas mobilensis

  Hayibor, Kennedy; Kwain, Samuel; Osei, Enoch; Nartey, Adwoa Padiki; Tetevi, Gilbert Mawuli; Owusu, Kofi Baffour-Awuah; Camas, Mustafa; Camas, Anil Sazak; Kyeremeh, Kwaku

  Sub-Sahara Africa is burdened with a high incidence of parasitic infections, including schistosomiasis, trypanosomiasis, trichomoniasis, and leishmaniasis. Currently, there is a rapid widespread development of resistance to prescription drugs for these neglected diseases. Microbes provide the largest chemical and biological diversity in drug discovery screening programs; therefore, our project seeks to explore microbes in the sub-region for new drugs. The oxylipin (9Z,11E)-13-oxooctadeca-9,11-dienoic acid (1) was isolated from the Ghanaian endophytic fungus, Penicillium herquei strain BRS2A-AR obtained from the leaves of a Laguncularia racemosa tree growing on the banks of the River Butre in the Western Regional wetlands of Ghana. Compound 1 was isolated on reverse phase HPLC at tR of 34.3 minutes. The structure of compound 1 was confirmed by a combination of mass spectrometry, 1D and 2D-NMR techniques. Compound 1 showed minimal antiparasitic activity when tested against Plasmodium falciparum 3d7 (IC50>100µM; aretesunate, IC50 36nm), Trypanosoma brucei brucei (IC50>100µM; Coptis japonica, IC50 8.20µM), Leishmani donovani (IC50>100µM; amphotericin B, IC50 0.32µM) and Leishmania major (IC50>100µM; amphotericin B, IC50 0.31µM). Compound 1 produced IC50 of 44.47µM when tested against Trichomonas mobilensis with metronidazole (IC50 5.20µM) as standard. These results show the potential to further engineer the structure of compound 1 into a potent anti-Trichomonas scaffold.

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  AtıfScopus13·OpenAlex13

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• MakaleAçık Erişim2019Scopus SJRQ2/r/munzur/3267
  Paenidigyamycin A, potent antiparasitic imidazole alkaloid from the ghanaian Paenibacillus sp. De2Sh

  Osei, Enoch; Kwain, Samuel; Mawuli, Gilbert Tetevi; Anang, Abraham Kwabena; Owusu, Kofi Baffour-Awuah; Camas, Mustafa; Camas, Anil Sazak; Ohashi, Mitsuko; Alexandru-Crivac, Cristina-Nicoleta; Deng, Hai

  A new alkaloid paenidigyamycin A (1) was obtained from the novel Ghanaian Paenibacillus sp. isolated from the mangrove rhizosphere soils of the Pterocarpus santalinoides tree growing in the wetlands of the Digya National Park, Ghana. Compound 1 was isolated on HPLC at tR = 37.0 min and its structure determined by MS, 1D, and 2D-NMR data. When tested against L. major, 1 (IC50 0.75 µM) was just as effective as amphotericin B (IC50 0.31 µM). Against L. donovani, 1 (IC50 7.02 µM) was twenty-two times less active than amphotericin B (IC50 0.32 µM), reinforcing the unique effectiveness of 1 against L. major. For T. brucei brucei, 1 (IC50 0.78 µM) was ten times more active than the laboratory standard Coptis japonica (IC50 8.20 µM). The IC50 of 9.08 µM for 1 against P. falciparum 3d7 compared to artesunate (IC50 36 nM) was not strong, but this result suggests the possibility of using the paenidigyamycin scaffold for the development of potent antimalarial drugs. Against cercariae, 1 showed high anticercaricidal activity compared to artesunate. The minimal lethal concentration (MLC) and minimal effective concentration (MEC) of the compound were 25 and 6.25 µM, respectively, while artesunate was needed in higher quantities to produce such results. However, 1 (IC50 > 100 µM) was not active against T. mobilensis.

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  AtıfScopus32·OpenAlex32

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