Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ2Cadmium-induced changes in haemato-immunological, oxidative stress and antioxidant parameters of Cyprinus carpio: Protective role of curcumin
Pala, Ayşegül; Eroğlu, Mücahit; Mişe Yonar, Serpil; İspir, Ünal; Yonar, Muhammet Enis
This study examined the adverse effects of sublethal concentrations of cadmium (Cd) and the potential of curcumin (CUR) to mitigate Cd-induced toxicity. We administered 1.5 and 3 mg/L of Cd and 100 mg/kg of CUR to fish (Cyprinus carpio) separately and in combination over 14 days. At the end of the experiments, various haematological and immunological parameters, oxidative stress levels and antioxidant parameters of fish were measured, and the differences between the groups were evaluated. Simultaneous CUR treatment with Cd reversed the changes in haematological parameters in fish, increased immune responses, reduced oxidative stress and normalized the impaired antioxidant enzyme activities. In summary, our study showed that dietary supplementation with CUR may be an effective strategy to reduce the toxic effects of Cd in carp.
- MakaleAçık Erişim2025Scopus SJRQ4Effects of chlorpyrifos on the freshwater mussel Unio elongatulus eucirrus (Bourguignat, 1860), with a focus on neurotoxicity and oxidative stress responses; [Efectos del clorpirifos en el mejillón de agua dulce Unio elongatulus eucirrus (Bourguignat, 1860), con especial atención a la neurotoxicidad y las respuestas al estrés oxidativo]
Zorlar, Faruk; Şeker, Engin; Pala, Ayşegül
This study set out to investigate the effects of the organophosphate insecticide chlorpyrifos (CPF) on certain biochemical parameters of the freshwater mussel Unio elongatulus eucirrus. Mussels were exposed to varying concentrations of CPF (10, 20, and 40 μg·L-1) for duration of 96 hours. Samples were collected from the mussels at the 24th and 96th hours of the experiment. The results indicated that exposure to CPF concentrations led to a decline in the activities of acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities and, accompanied by an increase in malondialdehyde (MDA), and decreased reduced glutathione (GSH) levels in mussels. In conclusion, the present study demonstrates that CPF caused AChE inhibition, formation of oxidative stress, and negative effects on certain antioxidant parameters in the freshwater mussel U. elongatulus eucirrus.
- MakaleAçık Erişim2025Scopus SJRQ2Determination of the Effect of Nanoparticle Copper on Navicula cryptocephala var. veneta by Biomarkers and Bioaccumulation Amounts
Serdar, Osman; Aydın, Ayşe Nur; Ölçülü, Abdüllatif; Çiçek Çimen, Işıl Canan; Parlak Ak, Tuba; Derman, Taner; Pala, Ayşegül; Yıldırım, Nuran Cikcikoğlu
Pollutants such as copper nanoparticles (CuNP), which form residues in the environment, especially in water environments, cause serious damage to the environment and the organisms living in the environment. In this study, Navicula cryptocephala var. veneta was selected as a model organism. The EC50 values of CuNP on Navicula cryptocephala var. veneta were determined as 4.94 mg\L and 3 different application concentrations were determined considering this ratio. The experiments were carried out in 3 replicates and samples were taken at 24 and 96 hours. At the end of 120 hours, samples were taken to determine the elimination values. CuNP accumulation amounts in the samples were measured by electrothermal atomic absorption spectrophotometer (ETAAS). For biomarker analysis, thiobarbituric acid (TBARS) and glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were performed using CAYMAN ELISA kit. According to the study data, as CuNP concentration and application time increased, the amount of accumulation in Navicula cryptocephala also increased. Increasing-decreasing changes were observed in elimination. CuNP caused oxidative stress in N. cryptocephala and caused changes in TBARS and GSH levels. In addition, it caused increases in SOD activities and statistically significant decreases (P<0.05) in CAT and GPx activities.
AtıfScopus2WoS1
İncele - MakaleAçık Erişim2022Güney Doğu Anadolu Bölgesi Sülük Faunasının Araştırılması
Pala, Ayşegül; Dörücü, Mustafa; Saglam, Naim; Sesli, Ahmet; Özbay, Özkan; Balci, Mustafa; Kılıç, Ali; Özbey, Nurten; Demir, Timur; Arca, Selami
Bu çalışma, Ocak 2012- Aralık 2014 tarihleri arasında Güney Doğu Anadolu Bölgesinde yer alan yedi ilde (Adıyaman, Batman, Diyarbakır, Gaziantep, Kilis, Mardin ve Şanlıurfa) bulunan akarsu, baraj, göl, gölet ve sazlık alan olmak üzere toplam 169 tatlısu habitatında sülük faunasının belirlenmesi amacıyla yürütüldü. Sülük örnekleri habitatın özelliğine göre birim zaman, birim çerçeve (Sülük toplama çerçevesi) ve Ekman kepçesi yöntemiyle toplandı. Toplam 169 sulak alanın 53’ünde sülük tespit edildi. Çalışma süresince toplanan sülük örnekleri su bulunan 0,5-1,0 L’lik pet bidonlarla laboratuvara taşındı. Sülük örnekleri önce % 10 etanol içinde bayıltıldı, sonra %70 alkol veya %4 formalin içinde tespit edildi. Toplanmış olan sülükler morfolojik özelliklerine göre stereo mikroskop altında teşhisi yapıldı. Bu çalışma sonunda , Güneydoğu Anadolu Bölgesi sulak alanlarında Albolossiphonia heteroclita, Albolossiphonia sp., Dina apathyi, Dina sp., Erpobdella octoculata, E. testacea, Erpobdella sp., Helobdella stagnalis, Hirudo sulukii, Placobdella costata ve Placobdella sp. olmak üzere altı soya ait toplam 11 farklı sülük türü belirlendi. Söz konusu çalışma kapsamında tesbit edilen H. sulukii dünya için 2016 yılında tanımlanmış bir tıbbi sülük türü olup, bölge halkı tarafından geleneksel ve tamamlayıcı tıp uygulamaları çerçevesinde kullanılmaktadır.
- MakaleYalnız künye2022Güney Doğu Anadolu Bölgesi Sülük Faunası Üzerine Araştırmalar
Özkan, Kadir; Demir, Timur; Balci, Mustafa; Sağlam, Naim; Dörücü, Mustafa; Özbey, Nurten; Kılıç, Ali; Pala, Ayşegül; Sesli, Ahmet; Özbay, Özkan
Bu çalışma, Ocak 2012- Aralık 2014 tarihleri arasında Güney Doğu Anadolu Bölgesinde yer alan yedi ilde (Adıyaman, Batman, Diyarbakır, Gaziantep, Kilis, Mardin ve Şanlıurfa) bulunan akarsu, baraj, göl, gölet ve sazlık alan olmak üzere toplam 169 tatlısu habitatında sülük faunasının belirlenmesi amacıyla yürütüldü. Sülük örnekleri habitatın özelliğine göre birim zaman, birim çerçeve (Sülük toplama çerçevesi) ve Ekman kepçesi yöntemiyle toplandı. Toplam 169 sulak alanın 53’ünde sülük tespit edildi. Çalışma süresince toplanan sülük örnekleri su bulunan 0,5-1,0 L’lik pet bidonlarla laboratuvara taşındı. Sülük örnekleri önce % 10 etanol içinde bayıltıldı, sonra %70 alkol veya %4 formalin içinde tespit edildi. Toplanmış olan sülükler morfolojik özelliklerine göre stereo mikroskop altında teşhisi yapıldı. Bu çalışma sonunda , Güneydoğu Anadolu Bölgesi sulak alanlarında Albolossiphonia heteroclita, Albolossiphonia sp., Dina apathyi, Dina sp., Erpobdella octoculata, E. testacea, Erpobdella sp., Helobdella stagnalis, Hirudo sulukii, Placobdella costata ve Placobdella sp. olmak üzere altı soya ait toplam 11 farklı sülük türü belirlendi. Söz konusu çalışma kapsamında tesbit edilen H. sulukii dünya için 2016 yılında tanımlanmış bir tıbbi sülük türü olup, bölge halkı tarafından geleneksel ve tamamlayıcı tıp uygulamaları çerçevesinde kullanılmaktadır.
- MakaleAçık Erişim2022Scopus SJRQ2Effect of chlorpyrifos on some biochemical changes in Cyprinus carpio: the protective effect of ellagic acid
Mişe Yonar, Serpil; Yonar, Muhammet Enis; Ural, Mevlüt Şener; Pala, Ayşegül
In this study, ameliorative effects of ellagic acid (EA) on oxidative stress induced by chlorpyrifos (CPF) in carp, Cyprinus carpio, were investigated. Fish were divided into six groups: C (no treatment), EA (100 mg kg fish−1), CPF-1 (0.040 mg L−1), CPF-2 (0.080 mg L−1), CPF-1 + EA, and CPF-2 + EA. CPF and EA were applied simultaneously for 14 days and, at the end of the study, liver, kidney, and gill samples were collected from fish. On the taken tissue samples, malondialdehyde (MDA) level and some antioxidant enzyme (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione-S-transferase (GST)) activities were evaluated. The results demonstrated statistically significant increases in the MDA levels of the CPF-1 and CPF-2 groups. On the other hand, the MDA levels were significantly decreased by EA administration. Also, CPF exposure caused statistically significant increases in the SOD and GST activities and statistically significant decreases in the CAT and GPx activities. However, treatment with EA reversed negative alterations in the SOD, CAT, GPx, and GST activities. Therefore, the results of this study results showed that simultaneous treatment with EA alleviates CPF-induced oxidative stress in fish.
AtıfScopus8WoS10OpenAlex10
İncele - MakaleAçık Erişim2021Scopus SJRQ1Ameliorative effect of Fennel (Foeniculum vulgare) essential oil on chlorpyrifos toxicity in Cyprinus carpio
Pala, Ayşegül; Serdar, Osman; Mişe Yonar, Serpil; Yonar, Muhammet Enis
Chlorpyrifos (CPF) is an organophosphate pesticide that is frequently and widely used to control both agricultural and domestic pests worldwide. In this study, the protective effect of Fennel (Foeniculum vulgare) essential oil (FEO) was investigated in carp (Cyprinus carpio) exposed to CPF. The fish were divided into six groups that one control group (no treatment) and five experimental groups (FEO (3ml/100g diet) group, CPF1 (0.023 mg/l) group CPF2 (0.046 mg/l) group, CPF1 (0.023 mg/l) plus FEO (3ml/100g diet) group, CPF2 (0.046 mg/l) plus FEO (3ml/100g diet) group). Blood and tissue (liver, kidney, gill, and brain) samples were taken from the fish at the end of 14 days of application. Hemoglobin (Hb) level, nitoblue tetrazolium (NBT) activity, and total immunoglobulin (TI) level were measured in blood samples of fish. Acetylcholinesterase (AChE) activity was determined in brain tissue while malondialdehyde (MDA) level, reduced glutathione (GSH) level, catalase (CAT), and glutathione peroxidase (GPx) activity were determined in liver, kidney, and gill tissues. The results showed that there was a significant decrease in Hb level, NBT activity, and TI levels in CPF-treated fish compared to the control group. In addition, increased in MDA levels and significant decreases in GSH level, AChE, CAT, and GPx activities were observed in CPF-treated groups. However, FEO-treated was showed a significant improvement in all parameters except AChE activity compared to CPF groups. These study findings showed that FEO could improve CPF-induced toxicity in C. carpio, except inhibition of AChE activity.
AtıfScopus6WoS6OpenAlex8İncele - MakaleAçık Erişim2020Malathionun Gammarus pulex (Tatlı Su Amphipodu)’te Asetilkolinesteraz Aktivitesi Üzerine Akut Etkisi
Serdar, Osman; Pala, Ayşegül; Aydin, Rahmi
Bu çalışmada, organofosfat insektisit malathionun subletal konsantrasyonlarının Gammarus pulex'in AChE aktivitesi üzerine akut (24 ve 48 saat) etkisi incelenmiştir. Bu amaçla, G. pulex'e malathionun iki farklı sublethal konsantrasyonu (0.1 ve 0.2 mg l-1 ) 24 ve 48 saat boyunca uygulandı. 24 ve 48 saat malathion maruziyetinin sonunda G. pulex örnekleri alındı. Alınan örneklerde AChE enzim aktivitesi ve protein düzeyi belirlenerek, spesifik AChE enzim aktivitesi hesaplandı. Malathionunun sublethal konsantrasyonları G. pulex'te AChE aktivitesininin zamana bağlı artan inhibisyonuna neden oldu. 0.1 mg l-1 malathion konsantrasyonuna maruz kalan grupta, AChE'nin inhibisyonu, kontrol ile karşılaştırıldığında, 24 saatte %50 ve 48 saatte %74 olarak tespit edildi. Benzer şekilde, 0.2 mg l-1 malathion konsantrasyonuna maruz kalan grupta da kontrol ile karşılaştırıldığında sırasıyla 24 ve 48 saatte % 60 ve % 68 AChE inhibisyonu gözlendi. Sonuç olarak, G. pulex'in malathiona 24 ve 48 saat boyunca maruz kalması AChE aktivitesinin yüksek oranda inhibe edilmesine neden olmuştur. Ayrıca, sonuçlar G.pulex'teki % 74'e kadar AChE inhibisyon seviyelerinin akut ölüme neden olmadığını ve G. pulex'teki AChE aktivitesinin ölçümünün akut malathion maruziyetinin ve etkilerinin biyobelirteci olacağını göstermektedir.
AtıfOpenAlex3TR Dizin1
İncele - MakaleYalnız künye2020Investigation of Endohelmints in The Fishes Hunted in Keban Dam Lake Çemişgezek Region (4th Region)
Şeker, Engin; Aktürk, Burcu; Pala, Ayşegül
This study was carried out on 210 fish, which were 52 Cyprinus carpio (Linnaeus, 1758), 49 Capoeta trutta (Heckel, 1843), 53 Lucio barbus mystaceus (Pallas, 1814) and 56 Lucio barbus esocinus (Heckel, 1843), which were hunted in the Keban Dam Lake Çemişgezek Region (4th Region) between July 2016 and December 2016. Three parasite species were encountered in 173 infested fish. After autopsy, two parasite types as Diplostomum sp. in the eyes fluid, Khawia sinensis in the intestine were identified in C. carpio. Two parasite species were identified in C. trutta, L. barbus esocinus and L. barbus mystaceus: Diplostomum sp. in eye fluids and Neoechinorhynchus rutili in their intestines. According to the results, it was determined that Diplostomum sp. was the most dominant parasite species with 73.33% prevalence and 8.97 density in 4 fish species examined. The prevalence rates of other identified parasite species N.rutili and K.sinensis were 40.95% and 15.38%, respectively, and densities were 7.11 and 2.25 respectively.
AtıfTR Dizin1İncele - MakaleAçık Erişim2020Scopus SJRQ1Effect of trichlorfon on some haematological and biochemical changes in Cyprinus carpio: The ameliorative effect of lycopene
Mişe Yonar, Serpil; Yonar, M. Enis; Pala, Ayşegül; Sağlam, Naim; Sakin, Fatih
Trichlorfon (TCF) is an antiparasitic agent used in aquaculture to treat external parasites and it belongs to the chemical class of organophosphates. This study was conducted to investigate the ameliorating effects of lycopene on TCF-induced toxicity in carp by evaluating haematological parameters and oxidant/antioxidant status. Fish were randomly divided into seven experimental groups. These groups were arranged as follows: control (no treatment) group, corn oil group, lycopene (10 mg kg−1) group, TCF-I (11 mg L−1) group, TCF-I (11 mg L−1) plus lycopene (10 mg kg−1) group, TCF-II (22 mg L−1) group and TCF-II (22 mg L−1) plus lycopene (10 mg kg−1) group. At the end of 14 days administration, blood and tissue (liver, kidney, and gill) samples were collected. Haematological changes [red blood cell (RBC) and white blood cell (WBC) count, haemoglobin (Hb) concentration, haematocrit (Ht) level, and erythrocyte indices: mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC)] were determined in the blood samples, while antioxidant parameters [(malondialdehyde (MDA) and reduced glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities] were evaluated in the liver, kidney and gill samples. The results demonstrated that all the haematological parameters were significantly decreased in the TCF-I and TCF-II groups when compared to the control group. Notable increases in the tissue MDA levels were observed in the TCF-treated groups. In contrast, significant decreases were observed in the tissue SOD, CAT and GSH-Px activities and GSH levels in the TCF-I and TCF-II groups. However, simultaneous treatment with lycopene provided a marked normalisation of the haematological and oxidant/antioxidant parameters compared with the TCF-I and TCF-II groups. The findings show that dietary lycopene supplementation might be helpful in abrogation of TCF toxicity by changing haematological conditions and antioxidant status in fish.
AtıfScopus34WoS28OpenAlex31İncele - MakaleYalnız künye2019Scopus SJRQ2The effect of a glyphosate-based herbicide on acetylcholinesterase (AChE) activity, oxidative stress, and antioxidant status in freshwater amphipod: Gammarus pulex (Crustacean)
Pala, Ayşegül
This study had determined the effect of glyphosate-based herbicide (GBH) on acetylcholinesterase (AChE) enzyme activity, oxidative stress, and antioxidant status in Gammarus pulex. Firstly, the 96-h LC50 value of glyphosate on G. pulex was determined and calculated as 403 μg/L. Subsequently, the organisms were exposed to sub-lethal concentrations (10, 20, and 40 μg/L) of the determined GHB for 24 and 96 h. The samples were taken from control and GBH-treated groups at 24 and 96 h of study and analysed to determine the malondialdehyde (MDA) and reduced glutathione (GSH) levels, the AChE, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) enzyme activities. In the G. pulex exposed to GBH for 24 and 96 h, the MDA level increased significantly (p < 0.05). The GSH level, the AChE, the CAT, and the GPx activities decreased compared with the control group (p < 0.05). G. pulex exposure to GBH for 24 h showed a temporary reduction in the SOD. GBH exposure led to oxidative stress in the G. pulex as well as affected the cholinergic system of the organism. These results indicated that the parameters measured may be important indicators of herbicide contamination in G. pulex.
AtıfScopus33WoS31OpenAlex35İncele - MakaleAçık Erişim2018Seasonal Variation of Acetylcholinesterase Activity as a Biomarker in Brain Tissue of Capoeta umbla in Pülümür Stream
Serdar, Osman; Pala, Ayşegül
This study was carried out between March 2015 and February 2016 with the aimof determining the seasonal variation of acetylcholinesterase (AChE) activity inbrain tissue of Capoeta umbla (Heckel, 1843) caught from Pülümür Stream(Tunceli). The seasonal fluctuations in the AChE activity of C. umbla whichcaught between the 39̊06' 19''N- 39̊36' 56''E (A) and 39̊11' 36''N-39̊41' 36''E (B)coordinates of Pülümür Stream, were studied over a period of one year. Theenzyme activity was higher in spring and summer than in autumn and winter. Inaddition, the minimum value for the AChE activity was determined in the winterseason and the maximum value was determined in the spring.
AtıfOpenAlex10TR Dizin1İncele - MakaleAçık Erişim2018Scopus SJRQ3Effect of Tunceli garlic on some immunological parameters in Cyprinus carpio exposed to chlorpyrifos
Pala, Ayşegül; Şeker, Engin; Yonar, M. Enis
In this study, the effect of Tunceli garlic (Allium tuncelianium) on hemoglobin (Hb) level, oxidative radical production of neutrophils (Nitoblue tetrazolium assay-NBT activity) and total immunoglobulin (TI) content in carp (Cyprinus carpio) exposed to chlorpyrifos (CPF) was investigated. The 96 hour LC50 value of CPF on C. carpio was calculated to as 0.230 mg/L. The fishes were exposed to sublethal concentration of chlorpyrifos (1/8 of LC50 value: 0.029 mg/L), and Tunceli garlic (20 and 40 g/kg diet) was simultaneously administered for 21 days. Blood samples were taken from the fishes at 7, 14 and 21 days and analysed to determine the Hb levels, the NBT activity and the TI content. There was a significant decrease in the Hb level, the NBT activity and the TI content of CPF-treated fish. However, Tunceli garlic reversed the Hb level, the NBT activity and the TI content. In conclusion, this study demonstrated that CPF had a negative effect on the immunological values of the fish. The simultaneous administration of Tunceli garlic was neutralised CPF-induced toxicity.
AtıfScopus5WoS4OpenAlex6İncele