Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2020Scopus SJRQ3/r/munzur/3501
  Genetic variation and molecular relationships taxa of conringia heist. EX FABR. (BRASSICACEAE) Based on rapd markers in Turkey

  Sevgndgk, Emre; Paksoy, Mehmet Yavuz; Aydoğan, Melike; Topseçer, Feyzanur

  In this study, genetic variation and phylogenetic analysis of 13 populations of 6 species belonging to Conringia genus spreading in Turkey were performed using RAPD markers. Genomic DNA isolation from the leaves of the Conringia plant samples was performed via using a commercial kit. Seven RAPD primers were used to identify the genetic diversity between the populations. Polymerase Chain Reaction (PCR) was performed using DNA samples and primers. PCR products were resolved using agarose gel electrophoresis and visualized under UV light. All gel images were analyzed, and the absence and presence of polymorphic bands were scored. The total of 34 DNA bands were detected by seven RAPD primers. PAUP 4.0b10 analysis program was used to calculate phylogenetic tree and genetic distances between the species. The phylogenetic tree was obtained using the UPGMA algorithm and it was composed of two clades. According to the PAUP analysis, the species having the closest distance between each other are C. planisiliqua (Ankara-AyaG) and C. planisiliqua (Ankara-Nallihan) with the value of 0.000 and those having the longest distance are C. grandiflora (Akseki Cukurkoy) and C. orientalis (Elazig-Baskil) with the value of 0.6000. The results suggest that the RAPD markers are useful tools to demonstrate the genetic relationships between populations of the Conringia species.

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• MakaleAçık Erişim2019/r/munzur/5031
  Phylogenetic Analysis of Chrysochamela (Fenzl) Boiss. (Brassicaceae) Taxa Growing in Turkey

  Paksoy, Mehmet Yavuz; Sevindik, Emre; Topseçer, Feyzanur; Aydoğan, Melike

  In this study, we performed a genetic diversity analysis using RAPD (Random AmplifiedPolymorphic DNA) and ISSR (Inter simple sequence repeat) markers for some Chrysochamelaspecies growing in Turkey. PCR was performed using RAPD and ISSR primers and genomic DNAsamples from each specimen, and the resulting bands were scored. Phylogenetic relationships andgenetic distances between the studied taxa were calculated using the PAUP analysis program.According to the RAPD analysis, the closest genetic distance was found to be 0.20000 between C.noeana populations, while the most distant values were found as 0.60000 between C. noeana and C.elliptica. The ISSR analysis also revealed the closest genetic distance as 0.34043 between C. noeanapopulations, while the most distant values were found to be 0.55319 between C. noeana and C.elliptica. UPGMA (unweighted pair group method with arithmetic mean) dendrogram generated forboth RAPD and ISSR analyses consisted of two groups. As a result, it was found that RAPD datawere more appropriate for comparison with the previous anatomical and morphological data of the studied taxa.

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