Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYalnız künye2025Scopus SJRQ1Naringenin Attenuates Testicular Toxicity and Apoptosis in Rats Chronically Exposed to Mercury Chloride
Kahramanoğullari, Merve; Erişir, Mine; Yaman, Mine; Kaya, Şeyma Özer; Ak, Tuba Parlak
Mercury is an industrial toxin with detrimental effects on the reproductive system. This study explored the impact of naringenin (NAR) on testicular toxicity and apoptosis in rats chronically exposed to mercury chloride (MC). The research involved 41 adult male Wistar-Albino rats aged about 2.5 months, which were allocated into 5 groups: group 1 was a control group, group 2 received 100mg/kg of NAR, group 3 was exposed to MC, group 4 received both MC and 50mg/kg of NAR, and group 5 received both MC and 100mg/kg of NAR. For the treatments, the control group took 1mL/kg saline via intraperitoneal (ip) injection and 1mL/kg corn oil via gavage. MC was administered at 0.4mg/kg/day to the MC groups via ip, and NAR was delivered in corn oil at 50 and 100mg/kg/day to the respective NAR groups via gavage. All treatments were performed over a period of 20 days, with MC administration starting 1 hour after NAR administration. The irregularity and loss in spermatogenic cell organization, degeneration and vacuolization in tubules, edema, and congestion in interstitial areas of the testicular tissue of the MC group were alleviated by NAR administration. In addition, the increase in Caspase 3, iNOS, and eNOS immunoreactivities of the MC group were alleviated by NAR administration. In conclusion, it was found that NAR had protective and therapeutic effects against oxidative damage, histopathological changes, apoptosis, and altered spermatological parameters resulting from MC exposure in the testis, and 100mg/kg NAR provided positive effects on altered histopathological analysis, spermatological parameters, and apoptosis.
AtıfScopus1WoS1
İncele - MakaleAçık Erişim2025Scopus SJRQ2Localization of phoenixin-14 and nesfatin-1, known as pleiotropic peptides, in some central and peripheral organs of female and male rats
Esen, Engin; Parlak Ak, Tuba; Yaman, Mine
Neuropeptides are diverse neuronal signaling molecules that regulate cellular and physiological functions. Phoenixin (PNX) and Nucleobindin 2 (NUCB2)/nesfatin-1, which are known to co-localize and interact in some hypothalamic nuclei such as arcuate nucleus (ARC) and paraventricular nucleus (PVN), have important pleiotropic roles. The aim of this study was to investigate the immunohistochemical distribution of these neuropeptides in biological tissues of adult female and male rats. For this purpose, a total of 12 Spraque-Dawley rats, 6 female and 6 male, aged 3–4 months, were sacrificed and hypothalamus, pituitary, brain, cerebellum, adrenal gland, ovary and testis tissues were taken. Serum gonadotropin-releasing hormone (GnRH), plasma PNX-14, and NUCB2/nesfatin-1 concentrations showed no statistically differences between sexes. PNX-14 immunoreactivity was more prominent in the ARC of male rats, whereas NUCB2/nesfatin-1 immunoreactivity was markedly higher in the PVN of females. In the anterior pituitary, PNX-14 and NUCB2/nesfatin-1 staining was more intense in females relative to males. PNX-14 immunoreactivity in the brain was quite high in female and male rats, and this immunoreactivity in the cerebellum was low in both sexes. NUCB2/nesfatin-1 exhibited moderate immunoreactivity in these organs in both sexes. In the adrenal cortex of both sexes, PNX-14 immunoreactivity was quite intense, while NUCB2/nesfatin-1 was a moderate intensity. PNX-14 immunoreactivity was more intense in the ovary, whereas NUCB2/nesfatin-1 immunoreactivity was more prominent in the testis. In conclusion, we demonstrated the immunohistochemical localization of PNX-14 and NUCB2/nesfatin-1 neuropeptides, in conjunction with serum GnRH levels, in organs associated with the hypothalamic-pituitary-gonadal (HPG) and hypothalamic-pituitary-adrenal (HPA) axes of adult male and female rats.
AtıfScopus1WoS1OpenAlex1
İncele - MakaleAçık Erişim2025Scopus SJRQ1ADAMTS-1, a multifunctional proteinase, in the uterus of both estrous cycle rats and ovariectomized rats can be regulated via hormones
Parlak Ak, Tuba; Yaman, Mine; Bayrakdar, Ali; Bulmus, Ozgur
Remodeling of the extracellular matrix (ECM) throughout the estrous cycle is one of the most striking features of the uterus. A disintegrin and metalloprotease with thrombospondin type I motifs (ADAMTS-1) is a metalloproteinase responsible for the degradation of some proteoglycans, which are ECM components. In this study, ADAMTS-1 distribution was analyzed in the uterus of ovariectomized rats administered 17β-estradiol (E2) and progesterone (P4) and in the uterus at different estrous stages. Ovariectomized (OVX) rats were subjected to single and combined E2 (0.2 mg/kg) and P4 (10 mg/kg) hormone replacement therapies. E2 was administered for 3 consecutive days, followed by E2, P4, or E2 + P4 for 4 consecutive days. The serum level of E2 decreased from the proestrus phase to the diestrus phase, but that of P4 was the highest in the estrus phase. During the estrus phase, the serum level of luteinizing hormone (LH) was the lowest and that of follicle-stimulating hormone (FSH) was the highest. P4 level increased significantly in the OVX + P4 and OVX + E2 + P4 groups compared with the OVX group. The serum levels of LH and FSH decreased in the OVX + E2 and OVX + P4 groups compared with the OVX group, and were the lowest in the OVX + E2 + P4 group. ADAMTS-1 immunoreactivity in luminal, glandular, and stromal cells of the uterus decreased from proestrus to diestrus. When immunoreactivity in hormone replacement groups was compared, weak immunoreactivity was observed in the OVX group. ADAMTS-1 immunoreactivity gradually increased in OVX + P4 and OVX + E2 groups, and was particularly notable in luminal, glandular, and stromal cells in the OVX + E2 + P4 group. ADAMTS-1 distribution was affected by the estrous cycle process and hormone replacement therapy in the OVX procedure.
AtıfScopus1OpenAlex1İncele - MakaleAçık Erişim2024Scopus SJRQ4Ginkgo biloba L. extract and flunixin meglumine attenuate sepsis– associated liver injury, oxidative stress, inflammation and apoptosis in rats; [El extracto de Ginkgo biloba L. y la flunixina meglumina atenúan la lesión hepática, el estrés oxidativo, la inflamación y la apoptosis asociados a la sepsis en ratas]
Ak, Tuba Parlak; Gul, Burcu; Yaman, Mine; Seven, Ismail; Dagoglu, Gurdal; Gul, Huseyin Fatih
Lipopolysaccharide (LPS), known as a stimulant of inflammation, causes acute liver injury by inducing the production of inflammatory mediators and oxidative stress. The purpose of this study is to determine whether of a nonsteroidal anti–inflammatory drug (NSAID) Flunixin meglumine (FM) and herbal an medicine Ginkgo biloba L. extract (GBE) show antioxidative, anti–inflammatory or antiapoptotic effects in liver tissue in LPS–induced hepatotoxicity. Animals were separated to 6 groups as control, sepsis (1 mg·kg-1, 7th day single dose, intraperitoneal (ip)), sepsis + FM (1 mg·kg-1, 7th day single dose, ip + 2.2 mg·kg-1 day, ip), sepsis + GBE (1 mg·kg-1, 7th day single dose, ip + 50 mg·kg-1 day, gavage), FM and GBE and the study continued for 7 days. Liver tissues taken from rats sacrificed were analyzed biochemically, histologically and immunohistochemically. Accordingly, LPS caused liver function markers alteration, inflammation, oxidative stress, and apoptosis, as well as histopathological changes in liver tissue. However, it was observed that LPS–induced changes were regulated by FM and GBE application. FM and GBE was demonstrated to have antioxidant, antiinflammatory and anti–apoptotic properties in LPS– induced hepatotoxicity.
- MakaleAçık Erişim2024Scopus SJRQ4Erythropoietin and Hypericum perforatum ameliorate Gentamicin–induced nephrotoxicity in rats; [La eritropoyetina y el Hypericum perforatum mejoran la nefrotoxicidad inducida por gentamicina en ratas]
Ak, Tuba Parlak; Sağıroğlu, Meltem; Korkmaz, Gizem Elif; Yaman, Mine
Gentamicin (GM), which causes nephrotoxicity, is an aminoglycoside antibiotic commonly prescribed to treat of gram–negative infections. Erythropoietin (EPO), which has several biological functions including neuroprotection, wound healing and nephroprotection, is a glycoprotein hormone that controls erythropoiesis. Hypericum perforatum (HP) is a medicinal herb with antibacterial and nephroprotective effects. The aim of this study is to demonstrate the efficacy of EPO and HP in GM nephrotoxicity using combined biochemical, histopathological and immunohistochemical evaluations together. A total of 36 male Spraque–Dawley rats were divided into as control, GM (100 mg·kg-1 day), GM+EPO, GM+HP, EPO (1000 IU·kg-1 three consecutive days apart) and HP (200 mg·kg-1 day) groups (n=6) and the experiment lasted for 9 days. GM–induced increased relative kidney weight and increased serum urea nitrogen (BUN), creatinine and urea levels were reduced by EPO and HP. EPO and HP reduced the level of malondialdehyde (MDA), which increased with GM application, and increased the activities of reduced glutathione (GSH), glutathione peroxidase (GSH–Px), and catalase (CAT). GM nephrotoxicity resulted in tubular degeneration, vacuolization and hyaline deposits, glomerular degeneration and interstitial mononuclear cell infiltration. EPO and HP attenuated these histopathological changes. Also, EPO and HP also reduced caspase–3 immunoreactivities, which increased with GM application. It was shown that EPO and HP have attenuating effects on GM–induced kidney injury, and especially the intense antioxidant content of HP has a regulatory effect on the negative consequences of oxidative stress.
- MakaleAçık Erişim2024Scopus SJRQ2Effects of naringenin on oxidative damage and apoptosis in liver and kidney in rats subjected to chronic mercury chloride
Kahramanoğullari, Merve; Erişir, Mine; Yaman, Mine; Parlak Ak, Tuba
Mercury chloride is a type of heavy metal that causes the formation of free radicals, causing hepatotoxicity, nephrotoxicity and apoptosis. In this study, the effects of naringenin on oxidative stress and apoptosis in the liver and kidney of rats exposed to mercury chloride were investigated. In the study, 41 2-month-old male Wistar-Albino rats were divided into five groups. Accordingly, group 1 was set as control group, group 2 as naringenin-100, group 3 as mercury chloride, group 4 as mercury chloride + naringenin-50, and group 5 as mercury chloride + naringenin-100. For the interventions, 1 mL/kg saline was administered to the control, 0.4 mg/kg/day mercury (II) chloride to the mercury chloride groups by i.p., and 50 and 100 mg/kg/day naringenin prepared in corn oil to the naringenin groups by gavage. All the interventions lasted for 20 days. Mercury chloride administration was initiated 1 h following the administration of naringenin. When mercury chloride and the control group were compared, a significant increase in plasma urea, liver and kidney malondialdehyde (MDA) levels, in kidney superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST) activities (p <.001), and a significant decrease in liver and kidney glutathione (GSH) levels (p <.001), in liver catalase (CAT) activity (p <.01) were observed. In addition, histopathological changes and a significant increase in caspase-3 levels were detected (p <.05). When mercury chloride and treatment groups were compared, the administration of naringenin caused a decrease aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH) (p <.01), urea, creatinine levels (p <.001) in plasma, MDA levels in liver and kidney, SOD, GSH-Px, GST activities in kidney (p <.001), and increased GSH levels in liver and kidney. The addition of naringenin-100 increased GSH levels above the control (p <.001). The administration of naringenin was also decreased histopathological changes and caspase-3 levels (p <.05). Accordingly, it was determined that naringenin is protective and therapeutic against mercury chloride-induced oxidative damage and apoptosis in the liver and kidney, and 100 mg/kg naringenin is more effective in preventing histopathological changes and apoptosis.
AtıfScopus13WoS14OpenAlex14İncele - MakaleYalnız künye2023Östrüs Siklusu Boyunca Sıçan Ovaryumunda ADAMTS-1 Metalloproteinazının Dağılımı
Parlak Ak, Tuba; Yaman, Mine; Bayrakdar, Ali; Bulmus, Ozgur
A Disintegrin and Metalloproteinase with Thrombospondins Motifs 1 (ADAMTS-1), ovaryumdaki foliküler gelişim sırasında folikül bütünlüğünü ve işlevselliğini sürdürmek amacıyla yeniden şekillenmede rol oynayan bir metalloproteinazdır. Bu çalışmada, dişi sıçanların farklı östrüs siklusu evrelerindeki ovaryum dokularında ADAMTS-1 immünolokalizasyonu incelendi. Vajinal smear yöntemi ile düzenli siklus gösteren hayvanlar tercih edilerek 18 adet Wistar Albino cinsi sıçan; proöstrüs (Grup I), östrüs (Grup II) ve diöstrüs (Grup III) olmak üzere üç gruba ayrıldı. Proöstrüs evresinde; folikül epitel hücreleri, teka hücreleri, lüteal hücreler ve intersitisyel hücrelerde yoğun ADAMTS-1 immünoreaktivitesi gözlendi. Östrüs evresinde, proöstrüs evresine göre bu ovarian hücrelerde daha az yoğun ADAMTS-1 immünoreaktivitesi belirlendi. Diöstrüs evresinde, boyama yoğunluğunun foliküler hücreler ile teka hücrelerinde östrüs evresine benzer olduğu, lüteal ve intersitisyel hücrelerde ise daha hafif olduğu saptandı. Histoskor verilerine göre, ADAMTS-1 immünoreaktivitesinin proöstrüs evresinde (2.84±0.13) östrüs (1.18±0.07) ve diöstrüs (1.08±0.10) evrelerine göre en yüksek değere (P<0.05) ulaştığı tespit edildi. Sonuç olarak; foliküler gelişim, ovülasyon ve regresyon süreçlerini içeren farklı siklus evrelerinin ekstrasellüler matriksindeki (ESM) yapısal değişikliklerle ilişkili olarak ADAMTS-1 immünoreaktivitesinde farklılıklar belirlendi.
- MakaleAçık Erişim2023Scopus SJRQ2Expression of phoenixin-14 and nesfatin-1 in the hypothalamo–pituitary–gonadal axis in the phases of the estrous cycle
Parlak Ak, Tuba; Yaman, Mine; Bayrakdar, Ali; Bulmus, Ozgur
Phoenixin-14 (PNX-14) and nucleobindin 2 (NUCB2)/nesfatin-1 are regulatory neuropeptides expressed in the hypothalamus. These neuropeptides can be effective in hormonal regulation of the hypothalamo–pituitary–gonadal (HPG) axis and reproductive functions. In the present study, the distribution of PNX-14 and NUCB2/nesfatin-1 in the hypothalamus, pituitary, ovary, and uterus tissues during the phases of the estrous cycle in female rats was investigated. Eighteen Wistar Albino rats determined among animals showing regular estrous cycle by vaginal smear method were divided into three groups: proestrus (Group I), estrus (Group II) and diestrus (Group III). Serum gonadotropin-releasing hormone (GnRH), plasma PNX-14, and NUCB2/nesfatin-1 concentrations were the highest, moderate, and lowest in estrus, diestrus, and proestrus phases, respectively. PNX-14 immunoreactivity in the supraoptic and arcuate nuclei of the hypothalamus and NUCB2/nesfatin-1 immunoreactivity in the paraventricular nuclei were particularly evident in the estrus phase. These neuropeptide immunoreactivities were decreased in different cells of anterior pituitary during proestrus compared with those during estrus and diestrus. PNX-14 immunoreactivity in the ovary, especially during the estrus phase, was diffuse and intense in the granulosa and luteal cells and oocytes, and it was few and weak in theca cells. In addition, NUCB2/nesfatin-1 immunoreactivity was abundant and strong in granulosa and luteal cells, theca and interstitial cells, and oocytes during estrus. In the estrus phase, PNX-14 immunoreactivity was strong in the glandular epithelial cells and stromal cells of the endometrium, also NUCB2/nesfatin-1 immunoreactivity was strong in the epithelial and glandular epithelial cells. As a result, when the estrous cycle was evaluated, it was concluded that the changes in the distribution of PNX-14 and NUCB2/nesfatin-1 at all phases were related to GnRH and that these neuropeptides showed the highest immunoreactivity especially in the HPG axis and uterus tissues of estrus rats.
AtıfScopus21OpenAlex22İncele - MakaleAçık Erişim2022Scopus SJRQ2Chrysin and flunixin meglumine mitigate overloaded copper-induced testicular and spermatological damages via modulation of oxidative stress and apoptosis in rats
Parlak Ak, Tuba; Tatli Seven, Pinar; Gur, Seyfettin; Gul, Burcu; Yaman, Mine; Seven, Ismail
This study aimed to evaluate the possible protective actions of chrysin and flunixine meglumine on testicular and spermatological injuries experimentally stimulated by copper. We separated 36 male Sprague–Dawley rats into six equal groups: control, chrysin, flunixine meglumine, copper, copper +chrysin and copper +flunixine meglumine. Chrysin (50 mg/kg/bw/po), flunixine meglumine (2.2 mg/kg/bw/ip) and copper (500 mg/kg/bw/po) were administered day to day for 21 days. Copper administration caused significant morphological, physiological and biochemical alterations compared to the control group, which are as follows: production of oxidative stress, thanks to rise in testis lipid peroxidation and fall in antioxidant enzyme concentrations, decrease in sperm quality and increase in morphologic sperm abnormalities, suppression of spermatogenesis and prominent alterations in the testis histomorphology and induction of apoptosis in the testis tissues. On the other hand, compared to the copper group, treatment with chrysin or flunixine meglumine significantly attenuated these alterations. In conclusion, chrysin and flunixine meglumine have benefits such as antioxidant, antiapoptotic and anti-inflammatory against copper-induced testicular and spermatological damages in rats via the modulation of oxidative stress and apoptosis. Consequently, chrysin is a natural product which has comparable therapeutic actions to flunixine meglumine on the male reproductive system.
AtıfScopus4OpenAlex4İncele - MakaleAçık Erişim2021Scopus SJRQ3Effects of Chrysin on Carbon Tetrachloride-Induced Testicular Damage in Rats: Biochemical, Histopathological and Immunohistochemical Evaluation
Parlak Ak, Tuba; Seven, Pinar Tatli; Seven, Ismail; Mutlu, Seda Iflazoglu; Yaman, Mine; Sur, Aslihan; Gul, Burcu
The aim of this study was to evaluate the effect of chrysin on testicular damage induced by carbon tetrachloride (CCl4) in rats. Twenty-four male Spraque-Dawley rats were divided into four groups as the control group, Chrysin group, CCl4 group, and Chrysin+CCl4 group. Chrysin (100 mg/kg/body weight/day) and CCl4 (0.5 ml/kg/body weight/day in corn oil (1:1)) were administered by gavage for 14 days. Compared to the control group, it was determined that malondialdehyde (MDA) level increased, reduced glutathione (GSH) level, and superoxide dismutase (SOD) and catalase (KAT) activities decreased in the CCl4 group. It was also determined that many testicular lesions were induced, Johnsen’s Mean Testicular Biopsy Score (JMTBS) decreased, and caspase-3 immunoreactivity increased in this group. Compared to the CCl4 group, it was determined that GSH level and SOD and CAT activities increased, MDA level decreased in the Chrysin+CCl4 group. It was also observed that testicular lesions were alleviated, JMTBS increased, and caspase-3 immunoreactivity was decreased in this group. As a result, it was concluded that chrysin might have beneficial effects in the treatment of CCl4-induced testicular toxicity as a result of its antioxidant and anti-apoptotic properties.
AtıfOpenAlex2
İncele - MakaleAçık Erişim2021Scopus SJRQ3The protective effect of chrysin against carbon tetrachloride-induced kidney and liver tissue damage in rats
Baykalir, Burcu Gul; Arslan, Aslihan Sur; Mutlu, Seda Iflazoglu; Parlak Ak, Tuba; Seven, Ismail; Seven, Pinar Tatli; Yaman, Mine; Gul, Huseyin Fatih
The aim of this study was to investigate the possible protective effects of chrysin on oxidative status and histological alterations against carbon tetrachloride (CCl4)-induced liver and kidney tissue in rats. The animals were randomly divided into four groups; the control, chrysin (100 mg/kg), CCl4 (0.5 ml/kg) and chrysin + CCl4 groups. Liver and kidney injuries were assessed by biochemical and histopathological examinations. The levels of malondialdehyde (MDA), reduced glutathione (GSH), and superoxide dismutase (SOD) activity were measured in tissues. Serum tumor necrosis factor-α (TNF-α), aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, and creatinine levels were also measured in blood samples. MDA, serum TNF-α, AST, ALT, urea, and creatinine levels (p < 0.05) were significantly higher, and SOD activity and GSH level were significantly (p < 0.05) lower in the CCl4 group than in the control group. Treatment with chrysin in the chrysin + CCl4 group decreased MDA, AST, ALT, creatinine, and TNF-α levels (p < 0.05), and increased SOD activity, GSH levels (p < 0.05), and serum TNF-α levels (p < 0.05). In addition, body weight change (BWC) (p < 0.05) and feed intake (FI) were significantly lower (p < 0.001) in the CCl4 group than in the control group. Moreover, treatment with chrysin increased BWC and FI in the chrysin + CCl4 group compared with that in the CCl4 group. These findings also confirmed by histopathological examination. The chrysin treatment ameliorated the CCl4-induced biochemical and pathological alterations. These results demonstrated that chrysin provided amelioration on the rat liver and kidney tissues CCl4-induced injury by increasing the antioxidant activity.
AtıfScopus20İncele - MakaleAçık Erişim2020Scopus SJRQ2Bee glue (propolis) improves reproductive organs, sperm quality and histological changes and antioxidant parameters of testis tissues in rats exposed to excess copper
Seven, Ismail; Tatli Seven, Pinar; Gul Baykalir, Burcu; Parlak Ak, Tuba; Ozer Kaya, Seyma; Yaman, Mine
This study was designed to determine the effects of propolis on the sperm quality, antioxidant and histological parameters in the testicular tissues of male Sprague Dawley rats exposed to excessive copper (Cu). In this aim, 24 rats were randomly divided into four groups as follows: the control, Cu, Propolis and Cu+Propolis. When compared to control group, Cu administration significantly decreased sperm motility and concentration, increased total abnormal sperm rate. It caused a significant induction the malondialdehyde (MDA), and reduction the superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) in testicular tissues. Also, it caused loss, disorganisation and vacuolation of the germinal epithelium, oedema of the interstitial tissues, proliferation of the interstitial cells, spilled immature spermatogenic cells in the lumen of some seminiferous tubules. A large number of active caspase-3-positive stained apoptotic cells and a significant decrease in Johnsen's testicular score were determined. However, significant ameliorations were observed in all sperm characteristics, MDA, SOD, CAT, GSH, seminiferous tubules, number of apoptotic cells and Johnsen's testicular score in Cu+Propolis group. Our results showed that oral supplementation of propolis had curative effect on the sperm quality, antioxidant and histological parameters in the testicular tissues of male Sprague Dawley rats exposed to Cu.
AtıfScopus39OpenAlex39İncele - MakaleYalnız künye2018Scopus SJRQ3The protective effects of propolis and flunixin meglumine on feed intake, antioxidant status and histological parameters in liver and kidney tissues against excess copper in rats
Parlak Ak, Tuba; Baykalır, Burcu Gül; Türkmen, Neşe Başak; Seven, Pinar Tatli; Seven, Ismail; Yaman, Mine
This experiment was conducted to determine the protective effects of flunixin meglumine and propolis on feed intake, antioxidant status and histological parameters in kidney and liver tissues in rats exposed to excessive copper. In this study individually housed thirty-six male Sprague-Dawley rats were used. Animals were randomly divided into six groups; control, copper sulphate (500 mg/kg BW/day, gavage), flunixin meglumine (2.2 mg/kg BW/day, ip), propolis (100 mg/kg BW/day, gavage), copper sulphate+flunixin meglumine (500 mg/kg BW/day of copper sulphate by gavage and 2.2 mg/kg BW/day of flunixin meglumine, ip) and copper sulphate+propolis (500 mg/kg BW/day of copper sulphate and 100 mg/kg BW/day of propolis by gavage). The study demonstrated that body weight change in the copper sulphate+propolis group significantly ameliorated in comparison with copper group (P<0.01). Propolis and flunixin meglumine administration significantly decreased MDA levels in the kidney and liver tissues, and serum TNF-α levels (P<0.001). Propolis supplementation to rats who were also treated with copper significantly increased the superoxide dismutase, catalase and reduced glutathione activities (P<0.001). Flunixin meglumine and propolis treatments decreased the copper-induced degenerative and necrotic changes with the apoptotic cells in the liver and kidney tissues. In conclusion, propolis appeared to ameliorate the adverse effects on feed intake, liver and kidney tissues seeing in the copper treatment and, apparently caused by the copper toxicity, by scavenging the free radicals and increasing activity of antioxidants.
AtıfTR Dizin1İncele - MakaleAçık Erişim2018Scopus SJRQ3The protective effects of propolis and flunixin meglumine on feed intake, antioxidant status and histological parameters in liver and kidney tissues against excess copper in rats; [Ratlarda bakır fazlalığına karşı yem tüketimi, antioksidan durum ve karaciğer ile böbrek dokularının histolojik parametreleri üzerine propolis ve fluniksin meglümin'in koruyucu etkileri]
Tatli Seven, Pınar; Gül Baykalir, Burcu; Parlak Ak, Tuba; Seven, İsmail; Başak, Neşe; Yaman, Mine
This experiment was conducted to determine the protective effects of flunixin meglumine and propolis on feed intake, antioxidant status and histological parameters in kidney and liver tissues in rats exposed to excessive copper. In this study individually housed thirty-six male Sprague-Dawley rats were used. Animals were randomly divided into six groups; control, copper sulphate (500 mg/kg BW/day, gavage), flunixin meglumine (2.2 mg/kg BW/day, ip), propolis (100 mg/kg BW/day, gavage), copper sulphate+flunixin meglumine (500 mg/kg BW/day of copper sulphate by gavage and 2.2 mg/kg BW/day of flunixin meglumine, ip) and copper sulphate+propolis (500 mg/kg BW/day of copper sulphate and 100 mg/kg BW/day of propolis by gavage). The study demonstrated that body weight change in the copper sulphate+propolis group significantly ameliorated in comparison with copper group (P<0.01). Propolis and flunixin meglumine administration significantly decreased MDA levels in the kidney and liver tissues, and serum TNF-α levels (P<0.001). Propolis supplementation to rats who were also treated with copper significantly increased the superoxide dismutase, catalase and reduced glutathione activities (P<0.001). Flunixin meglumine and propolis treatments decreased the copper-induced degenerative and necrotic changes with the apoptotic cells in the liver and kidney tissues. In conclusion, propolis appeared to ameliorate the adverse effects on feed intake, liver and kidney tissues seeing in the copper treatment and, apparently caused by the copper toxicity, by scavenging the free radicals and increasing activity of antioxidants.
AtıfScopus10OpenAlex9İncele - MakaleYalnız künye2016Protective Effect of Ginkgo Biloba Extract 761 on Di-(2-ethylhexyl) Phthalate-Induced Testicular Toxicity in Prepubertal Rats
Parlak Ak, Tuba; Yaman, Mine
Bu çalışmada, ratlara farklı sürelerde aynı dozda di-(2-etilhekzil) fitalat (DEHP) verilerek oluşturulan testiküler hasar üzerine ginkgo biloba ekstrakt 761 (EGb 761)'in koruyucu etkisi incelendi. Yetmiş iki adet 28 günlük Wistar Albino cinsi rat; kontrol, DEHP, DEHP + EGb 761 ve EGb 761 gruplarına ayrıldı. Çalışmanın 7., 14., ve 28. günlerinde hayvanlar dekapite edildi. DEHP uygulamasının kontrol grubu ile karşılaştırıldığında; malondialdehit (MDA), glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) seviyelerinde, testiküler dokuda dejenerasyon, nekroz, atrofi, disorganizasyon, Leydig hücre hiperplazisi ve bazal membran kalınlaşması ile birlikte apoptotik indekslerde belirgin artışlara sebep olmasına karşın, testislerin ağırlıklarında, seminifer tubüllerin çaplarında, germinal hücre tabakası kalınlıklarında, serum testosteron ve glutatyon (GSH) seviyelerinde belirgin azalışlara neden olduğu belirlendi. DEHP uygulanan ratlara EGb 761 verilmesi ile bozulmuş oksidan/antioksidan dengesinde, azalmış testosteron konsantrasyonunda, indüklenmiş testiküler apoptoziste belirgin gelişme ve testiküler dokunun histopatolojik görünümünde kademeli olarak düzelme sağlandığı gözlendi. DEHP' ın uygulanan süre ve dozda testis dokusu için son derece toksik olduğu ve ciddi yapısal hasara sebep olduğu; antioksidan etkiye sahip olan EGb 761'in ise büyük ölçüde bu hasarın önüne geçtiği tespit edildi
AtıfTR Dizin1İncele - MakaleAçık Erişim2011Scopus SJRQ2Distribution of neuropeptides in endocrine and exocrine pancreas of long-legged buzzard (Buteo rufinus): An immunohistochemical study
Bayrakdar, Ali; Yaman, Mine; Atalar, Omer; Tarakci, Berrin Gencer; Ceribasi, Songul
This study aimed to determine the existence and distribution of certain neuropeptides in endocrine and exocrine pancreas of the long-legged buzzard by using immunohistochemical methods. SOM-14-, NPY- and CGRP-IR endocrine cells were determined in both central and peripheral regions in A-islets within the pancreas, while SP-IR endocrine cells were found only in the central region, and CCK-8- and galanin-IR endocrine cells were only detected in peripheral region. On the other hand, in B-islets; SP-, NPY- and CGRP-IR endocrine cells were determined in both central and peripheral regions, while SOM-14- CCK-8- and galanin-IR endocrine cells were found only in the peripheral region. In addition; SOM-14-, NPY-, CGRP-. CCK-8- and galanin-IR cells were also observed in exocrine pancreas. This distribution pattern in the pancreas of the long-legged buzzard demonstrates that neuropeptides perform their probable affects through endocrine and/or paracrine mechanisms. In conclusion, the existence and distribution of neuropeptides in the pancreas of long-legged buzzard have been introduced in this study for the first time and this bird species has also been found to differ from other types of avian species. (c) 2010 Elsevier B.V. All rights reserved.
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