Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYalnız künye2014Scopus SJRQ3Assessment of immunomodulator biomarkers (Tnf-α, Il-1β and Il-6) in liver of Capoeta umbla for biomonitoring of pollution in Uzuncayir Dam Lake (Tunceli, Turkey)
Yildirim, N. C.; Danabas, S.
This study was aimed to monitor water pullution of Uzuncayir Dam Lake using the changes of IL-6, IL1 beta and TNF-alpha levels in Capoeta umbla (Heckel, 1843) liver tissue at ten stations in March and September 2011. In this study C. umbla (Heckel, 1843) was used as the indicator organism. Tumor necrosis factor-alpha (TNF-alpha), interlekuin1 beta (IL-1 beta) and interleukin-6 (IL-6) levels were determined in samples of the liver tissue by ELISA kit. The lowest mean IL-1 beta levels were found at station 6. The mean IL-1 beta was reached its maximum level at station 2. The difference between the mean levels of IL-6 was found to be significant (p<0.05) amoung stations. The IL-6 levels were significantly increased in September at stations 1, 2, 7 and 8 (p<0.01) compared to the values in March. The mean levels of TNF-alpha were found to be significant (p<0.05) amoung stations. The TNF-alpha levels significantly decreased in September at stations 1 and 9 (p<0.01). TNF-alpha, IL-1 beta and IL-6 levels in C. umbla can be used as early diagnostic indicators against adverse environmental events and useful and reliable bioindicators in determining the pollution of the aquatic ecosystem.
AtıfWoS16
İncele - MakaleYalnız künye2014Scopus SJRQ2Essential Oil Composition, Antioxidant and Antifungal Activities of Salvia sclarea L. from Munzur Valley in Tunceli, Turkey
Yuce, E.; Yildirim, N.; Yildirim, N. C.; Paksoy, M. Y.; Bagci, E.
The essential oil composition and in vitro antioxidant and antifungal activity of the Salvia sclarea L. from Munzur Valley in Tunceli, Turkey were evaluated in this research. The in vitro antifungal activity of ethanol, hexane and aqueous extracts of S. sclarea against pathogen fungi Epicoccum nigrum and Colletotrichum coc-codes were investigated. The essential oil of aerial parts of S. sclarea was obtained by hydrodistillation and was analysed by GC and GC-MS. Total antioxidant status was determined by using Rel assay diagnostics TAS assay kit (Lot. RL024) by Multiscan FC (Thermo). 33 compounds were identified representing the 85.0% of the total oil. The most abundant components (> 5%) of the S. sclarea essential oils were caryophyllene oxide (24.1%), sclareol (11.5%), spathulenol (11.4%), 1H-naphtho (2,1,6) pyran (8.6%) and beta-caryophyllene (5.1%). The best antifungal and antioxidant effect was seen in ethanolic S. sclarea extract. It can be said that Salvia sclerae could be used as natural antioxidant.
AtıfWoS26
İncele - MakaleYalnız künye2013Scopus SJRQ3EVALUATION OF ANTIOXIDANT PROPERTIES, ELEMENTAL AND PHENOLIC CONTENTS COMPOSITION OF WILD NETTLE (Urtica dioica L.) FROM TUNCELI IN TURKEY
Yildirim, N. C.; Turkoglu, S.; Ince, O. K.; Ince, M.
Wild nettle (Urtica dioica L.) types were sampled from different geographical regions in Tunceli (Turkey) to determine their mineral, vitamin, phenolic contents and their antioxidant properties. The total phenol varied from 37.419 +/- 0.380 to 19.182 +/- 1.00 mg of GAEs g(-1) of dry nettle. The highest radical scavenging effect was observed in Mazgirt parting of the ways 7.5 km with 33.70 +/- 0.849 mg mL(-1). The highest reducing power was observed in the nettles from Mazgirt parting of the ways 7.5 km. Among the various macronutrients estimated in the plant samples, potassium was present in the highest quantity followed by calcium and phosphate. Kaempferol and resveratrol were not determined in some nettle samples but rutin levels were determined in all samples. Vitamin A concentrations were ranged between 13.64 +/- 1.90 and 5.74 +/- 1.00 (mg kg(-1) dry weight). These results show that Urtica dioica L. collected from Tunceli in Turkey could be considered as a natural alternative source for food, pharmacology and medicine sectors.
AtıfWoS11İncele - MakaleYalnız künye2013Scopus SJRQ3THE USE OF LIVER ANTIOXIDANT DEFENSE SYSTEM OF CAPOETA UMBLA (HECKEL, 1843) AS BIOMARKER FOR MONITORING IN WATER QUALITY OF UZUNCAYIR DAM LAKE, TUNCELI, TURKEY
Yildirim, N. C.; Ergin, C.
Domestic wastewaters of Tunceli, are directly discharged to Munzur and Pulumur Rivers without any pre-purification process. The purpose of this study is to reveal the size of pollution caused by domestic wastewaters together with natural contamination by the drainage area of the Pulumur and Munzur Rivers and to monitor physiological changes in fish captured from Uzuncayir Dam Lake. In fish samples, superoxide dismutase, catalase, glutathione peroxidase activities and levels of glutathione and malondialdehyde were determined. Our studies showed that the antioxidant system of Capoeta umbla could be used as biomarker of exposure to aquatic pollutants in Uzuncayir Dam Lake.
AtıfWoS3İncele - MakaleYalnız künye2013Scopus SJRQ3POMEGRANATE SEED EXTRACT ATTENUATES CHEMOTHERAPY-INDUCED LIVER DAMAGE IN AN EXPERIMENTAL MODEL OF RABBITS
Yildirim, N. C.; Kandemir, F. M.; Ceribasi, S.; Ozkaraca, M.; Benzer, F.
AtıfOpenAlex14İncele - MakaleYalnız künye2011Scopus SJRQ3Some Haematological and Biochemical Parameters in Common Carp (Cyprinus carpio L., 1758) in Munzur River, Tunceli, Turkey
Gulec, A. K.; Yildirim, N. C.; Danabas, D.; Yildirim, N.
Haematological parameters are indicators of the response of the animal to the environment and used as reliable indicators of fish health status to detect physiological changes following different stress conditions. This study was carried out to evaluate some haematological and biochemical parameters of Cyprinus carpio caught in Munzur River, Tunceli, Turkey. The values of total red blood cell count (RBC), haematocrit (HCT), haemoglobin concentration (HGB), mean corpuscular volume (MCV), mean cellular haemoglobin content (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet count (PLT), mean platelet volume (MPV), plateletcrit (PCT), platelet distribution width (PDW) were measured. Levels of amylase, high-density lipoprotein cholesterol (HDL-C), cholesterol (CHOL), albumin (ALB), total protein (T-PROT), direct bilirubin (D-BIL), total bilirubin (T-BIL), gamma glutamyle tranferas (GGT), urea, creatinine, glucose (GLU), triglycerides (TRIG), uric acid (Uric-A), sodium, potassium, calcium, phosphorus and iron, chlorine were determined as 1.02 10(12)/L, 17.90 %, 11.72 g/L, 198.00 fL, 166.82 pg, 89.50 g/dL, 112.80 10(9)/L, 8.06 fL, 0.055 %, 17.59 fL, 87.20 U/L, 220.24 mg/dL, 767.40 mg/dL, 1.60 g/dL, 4.68 g/dL, 1.83 mg/dL, 3.046 mg/dL, 12.00 U/L, 9.20 mg/dL, 0.72 mg/dL. 504.40 mg/dL, 381.00 mg/dL, 2.76 mg/dL, 134.20 mmol/L, 1.90 mmol/L, 13.78 mg/dL, 17.96 mg/dL, 262.60 g/dL, 81.80 mmol/L, respectively. We assume that variation in values of blood indices may be a defensive mechanism against pollution.
AtıfWoS8İncele - MakaleYalnız künye2011Scopus SJRQ2Toxic Elements in Animal Products and Environmental Health
Kaplan, O.; Yildirim, N. C.; Yildirim, N.; Cimen, M.
In the higher organisms, the intake of trace elements occurs mainly through the respiratory system or through the food chain. Particularly, many dangerous elements or compounds, such as metals accumulate along the food chain. In this way, the food chain becomes the main gateway for persistent toxicants to enter higher organisms. Furthermore, these toxic elements generally have an anthropogenic origin and thus their concentrations in the environment grow with the increase of urban, agricultural and industrial emissions. With increasing environmental pollution, a toxic metal exposure assessment study is necessary. Toxic elements are not metabolized at all. Some metals irreversibly are bound to body tissues, e.g., lead to bone or cadmium to kidneys. This review updates the information on carry-over of toxic substances from feed to food of animal origin (meat, organs, milk and eggs). The control of environmental contaminants that may cause residues in food of animal origin is sometimes quite difficult and expensive and is addressed with special attention in this review.
AtıfWoS27OpenAlex65İncele - MakaleYalnız künye2011Scopus SJRQ3EVALUATION OF ENVIRONMENTAL POLLUTION AT MUNZUR RIVER OF TUNCELI APPLYING OXIDATIVE STRESS BIOMARKERS IN CAPOETA TRUTTA (HECKEL, 1843)
Yildirim, N. C.; Benzer, F.; Danabas, D.
Aim of this study was to determine the extent of pollution in the Munzur River in Turkey was determined. The antioxidant (reduced glutathione (GSH), glutathione peroxidase (GSH-Px) catalase (CAT)) and the oxidant (MDA) status of hepatopancreas, gills, muscle, heart and kidney tissues of C. trutta were chosen as bioindicators. The fish samples were caught from contaminated (Station I) and uncontaminated (Station II) stations in Munzur River during April 2010. GSH levels were lower in gill, muscle and heart of the C. trutta collected at the contaminated station (p<0.05). The MDA levels were significantly higher in gill, muscle, kidney and heart of the C. trutta from the contaminated station (p<0.05). The CAT activity was significantly increased in the gill tissue at the contaminated station (p<0.05). GSH-Px activity was significantly decreased (p<0.05) in gill, muscle, kidney and heart tissues samples from C. trutta collected at the contaminated station compared to those collected from the uncontaminated station. Overall, the results demonstrate that alteration in the antioxidant enzymes, glutathione system and induction of lipid peroxidation reflects the presence of pollution, which may cause oxidative stress in the C. trutta from Munzur River. The results provide evidence those enzymatic and non-enzymatic biomarkers of oxidative stress.
AtıfWoS24İncele - MakaleYalnız künye2011Scopus SJRQ3Use of Biochemical Markers in Capoeta trutta (Heckel, 1843) for the Assessment of Aquatic Pollution in Munzur River, Tunceli, Turkey
Yildirim, N. C.; Danabas, D.; Ergin, C.
The potential utility of biomarkers for monitoring both environmental quality and the health of organisms inhabiting polluted ecosystems has received increasing attention during the recent years. In this study. it was aimed to investigate the possible effect of pollution on biochemical parameters of Capoeta trutta captured in two environments with different levels of pollution in Munzur River, Tunceli, Turkey. One of them, hereby named reference site, there is no sewage discharge. Other side is denominated as polluted site is characterized by discharge of domestic sewage. Levels of amylase, alkaline phosphatase, lactate dehydrogenase, high-density lipoprotein cholesterol, cholesterol, albumin, total protein, direct bilirubin, total bilirubin, gamma-glutamyl tranferase, aspartate aminotransferase, alanine aminotransferase, urea, creatinine, glucose, triglycerides and uric acid were checked in blood samples. Plasma biochemical changes showed significantly differences between reference and polluted sites in alkaline phosphatase, lactate dehydrogenase, high-density lipoprotein cholesterol, cholesterol, alanine aminotransferase and glucose values. These results indicate that both domestic waste water and agricultural activities can alter the serum biochemical contents of Copeata trutta fish in Munzur River. Continuous production of pollutants resulting in impaired ability to respond to different environment stress factors and diseases. Present groups of researchers suggest that pollution levels should be monitored regularly.
AtıfWoS3İncele - MakaleYalnız künye2011Scopus SJRQ3Physico-chemical and Microbiological Water Quality Assessment of Perisuyu River, Tunceli, Turkey
Kaplan, O.; Yildirim, N. C.; Yildirim, N.; Akyol, E. A.
Generally, the water quality depends on its chemical and microbiological conditions. So, in this study, the chemical and microbiological characteristics of the Perisuyu River through 4 year long surveillance in Tunceli. Turkey were studied. Sampling was carried out four times during the year. Nitrate, nitrite, ammonium concentrations, total dissolved solid, total coliform counts were determined and the presence of Escherichia coli, important water quality indicator, was tested. Generally in water samples E. coli and total coliform were found very high and nitrate, nitrite and ammonium concentrations were low. In 2008 August and 2009 February water pH was higher than the World Health Organization limit value. Electrical conductivity was higher in 2004 May, December and 2008 February.
AtıfWoS3İncele - MakaleYalnız künye2011Scopus SJRQ3Levels of copper in liver, muscle and gill tissues in Capoeta trutta (Heckel, 1843) from Munzur River, Turkey
Danabas, D.; Benzer, F.; Kaplan, O.; Yildirim, N. C.
Heavy metal pollution of waters is a major environmental problem. Due to their toxicity, long persistence, bioaccumulative and nonbiodegradable properties in the food chain, heavy metals constitute a core group of aquatic pollutants. Capoeta trutta is an economically important fish and the object of significant commercial fishery in the Munzur River. Levels of Cu were measured in samples of the muscle, gill and liver tissues of C. trutta from Munzur River. In liver copper levels were higher at Station I (6.15 mg kg(-1)) polluted by domestic waste than Station II (5.48 mg kg(-1)) (P<0.05). The highest copper level (7.25 mg kg(-1)) was measured in muscle tissue at Station II. There was found no statically differences between stations in gill and muscle (P>0.05). It is suggested that ecological condition of freshwater fish and contamination levels in Munzur River must be regularly monitored.
AtıfWoS7İncele - MakaleYalnız künye2011Scopus SJRQ3Effect of Pomegranate Seed Extract on Free Radical Damage and Antioxidant Activity Under Cisplatin-Induced Oxidative Stress Conditions in Rabbit Testes
Benzer, F.; Kandemir, F. M.; Yildirim, N. C.; Ozan, S. T.
The aim of this study is to evaluate the protective effect of pomegranate seed extract on cisplatin-induced oxidative stress in rabbits testes. Rabbits were divided into three groups; (i) being control group, (ii) cisplatin group and (iii) cisplatin + pomegranate seed extract group. The level of malondialdehyde and glutathione, the activity of catalase, glutathione peroxidase, were measured in testes tissue of rabbits. Malondialdehyde levels were increased depending on cisplatin. In the case of cisplatin + pomegranate seed extract, malondialdehyde levels were found to be lower than cisplatin group (p < 0.05). In cisplatin group, catalase, glutathione peroxidase activity and glutathione levels were decreased when compared to control but in cisplatin + pomegranate seed extract group, catalase, glutathione peroxidase activity and glutathione levels were found higher than cisplatin group (p < 0.05). The pomegranate seed extract supplementation may play a protective role or decrease the side effects of cisplatin-induced testes toxicity from cancer medicines.
AtıfWoS5İncele - MakaleYalnız künye2011Scopus SJRQ2Compensatory effects of curcumin on cisplatin-induced toxicity in rabbit testis
Kandemir, F. M.; Benzer, F.; Yildirim, N. C.; Ozdemir, N.
Curcumin, a widely used spice and colouring agent in food, has been shown to possess potent antioxidant, antitumor promoting and anti-inflammatory properties in vitro and in vivo. The present study was designed to investigate the protective effects of curcumin on changes in the levels of lipid peroxidation and endogenous antioxidants induced by cisplatin (cis-diamminedichloroplatinum II, CDDP) in the testiclular tissue of rabbits. 18 healthy male New Zealand white rabbits were equally divided into three groups of six rabbits each, control, cisplatin, and cisplatin+curcumin. The degree of protection produced by cisplatin was evaluated by determining the level of malondialdehyde (MDA) and glutathione (GSH), the activity of catalase (CAT), glutathione peroxidase (GSH-Px), were estimated from testes homogenates. MDA levels were increased with cisplatin compared to control but in cisplatin+curcumin group, MDA levels were found to be lower than cisplatin group (p<0.05). The activity of CAT and GSH-P-X was decreased in cisplatin and cisplatin+curcumin groups compared to control (p<0.05). In the case of cisplatin+curcumin CAT and GSH-Px activity were increased compared to cisplatin group (p<0.05). GSH levels were decreased with cisplatin but administration cisplatin+curcumin increased the levels of GSH comared to cisplatin group (p<0.05). In the present study, co-administration of curcumin with cisplatin prevented the damage to testes induced by this drug and may be considered as a potentially useful candidate in the combination chemotherapy with cisplatin.
AtıfWoS22İncele - MakaleYalnız künye2011Scopus SJRQ3MALONDIALDEHYDE LEVELS AND GLUTATHIONE PEROXIDASE ACTIVITIES IN THE TISSUES OF FRESHWATER CRAYFISH (ASTACUS LEPTODACTYLUS ESCH 1823) IN CULTURE CONDITION
Kandemir, F. M.; Benzer, F.; Erisir, M.; Yildirim, N. C.
The objective of the study was to characterize the effects of sex on lipid peroxidation (as malondialdehyde, MDA), antioxidant defence system (as glutathione peroxidase, GSH-Px) in the hepatopancreas, gonad and muscle tissues of freshwater crayfish, Astacus leptodactylus, in culture condition. The crayfish used in the present study was provided from Keban Dam Lake population of A. leptodactylus. 34 males and 30 females crayfish in different size were hold in stock ponds (6 x 6 x 0.5 m). This study was carried out between June 18, 2004 and December 1, 2004 at the crayfish reproduction unit of Aquaculture Faculty of Firat University, Elazig, Turkey. Plastic pipes (20 cm in length and 7 cm in diameter) were provided as shelters for the crayfish. Crayfish were fed 2 % of their total wet weight daily with prepared ration. Crayfish were exposed to natural photoperiod. During the study, the mean water temperature, dissolved oxygen and mean pH were 4 degrees C, 6.28 +/- 0.32 mg L-1 and 7.82 +/- 0.17, respectively. MDA and GSH-Px levels according to sex and tissue-species were determined. MDA level in the hepatopancreas and gonad of females were higher than males, except muscle tissue (P<0.001). However, the GSH-Px activities in the hepatopancreas and gonad of males were higher than females, except muscle tissue (P<0.001). As a result, MDA and GSH-Px levels in the hepatopancreas, gonad and muscle tissues of freshwater crayfish, Astacus leptodactylus, in culture condition changed both tissues and sex.
AtıfWoS4İncele - MakaleAçık Erişim2010Scopus SJRQ1The effect of adrenomedullin and cold stress on interleukin-6 levels in some rat tissues
Yildirim, N. C.; Yurekli, M.
P>Stress known to stimulate sympathetic activity, as well as the hypothalamic-pituitary-adrenal axis (HPA), produces a significant increase in adrenomedullin (AdM) levels, suggesting a regulatory or protective role for AdM in countering HPA activation that follows a variety of stressors. Stressors can modulate the secretion of proinflammatory cytokines. Interleukin (IL)-6 is a potent activator of the HPA and appears to play a pathogenic role in conditions related to stress. In the present study, we investigated the administration of AdM on IL-6 levels in cold exposed rats. Male Wistar rats were divided into four groups as control, adrenomedullin treatment, cold stress and cold stress+adrenomedullin-treated groups. In the adrenomedullin-treated group, animals received intraperitoneal (i.p.) injection of adrenomedullin (2000 ng/kg body weight) once a day for a week. For the cold stress exposure the rats were kept in separate cages at 10 degrees C for a week. Control group rats were kept in laboratory conditions. The concentration of IL-6 was determined using an enzyme-linked immunosorbent assay (ELISA) kit. When compared to control, IL-6 levels increased significantly in the cold stress- and adrenomedullin-treated groups (P < 0 center dot 05). Administration of AdM in addition to cold stress decreased IL-6 levels in lung and liver, but increased in brain and heart when compared to control (P < 0 center dot 05). The results suggest that cold stress may induce increase of rat proinflammatory cytokine IL-6 and adrenomedullin may play a regulatory or protective role for cold stress.
AtıfWoS24OpenAlex26İncele