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- MakaleYalnız künye2026Evaluation of the Effects of Chrysin in Rats Developed with Lead Acetate-Induced Neurotoxicity Using Biochemical, Histopathological, and Immunohistochemical Methods
Kandemir, Fatih Mehmet; Benzer, Fulya; Küçükler, Sefa; Yildirim, Serkan; Kandemir, Özge; Dalkılınç, Elif; Uçar, Aybüke Gözde Gültekin
Lead (Pb) is a heavy metal with high toxicity that can cross the blood-brain barrier and accumulate in brain tissue, causing serious neurological disorders. The aim of this study is to evaluate the potential protective effect of chrysin (CHR) against Pb-induced neurotoxicity. Thirty-five male Sprague Dawley rats were divided into five groups: Control, CHR (50 mg/kg), Pb, Pb+CHR (25 mg/kg), and Pb+CHR (50 mg/kg). Rats were administered CHR (25 or 50 mg/kg) and Pb (30 mg/kg) orally for 7 days. Twenty-four hours after the last treatment, the rats were sacrificed under mild sevoflurane anesthesia by decapitation, and brain tissue samples were collected. Biochemical and histopathological methods were used to measure cholinergic enzyme activity, DNA damage, and oxidative stress parameters in brain tissue. CHR reduced levels of glial fibrillary acidic protein (GFAP), acetylcholinesterase (AChE), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) caused by Pb. Due to its antioxidant properties, CHR decreased Pb-induced lipid peroxidation while increasing glutathione (GSH) levels and the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx). Based on biochemical and immunohistochemical analyses, CHR reduced cyclooxygenase-2 (COX-2), myeloperoxidase (MPO), nuclear factor kappa-B (NF-κB), neuronal nitric oxide synthase (nNOS), prostaglandin E2 (PGE-2), and p53 levels. These findings suggest that CHR may exert a therapeutic effect by alleviating oxidative stress and inflammation induced by Pb-induced neurotoxicity.
- MakaleAçık Erişim2021Investigation of Chrysin Effects on Lead Asetat-Induced Liver Damage in Rats
Benzer, Fulya; Kandemir, Fatih Mehmet; Yildirim, Serkan; Coşkun, Pınar; Küçükler, Sefa
This study was conducted to investigate the effects of chrysin (CH) on lead acetate (PbA) inducing liver injury. In this study, 35 male Sprague Dawley rats were divided into 5 groups as 7 rats in each group. Group 1 (Control): Saline was given orally. Group 2 (CH group): CH (50 mg kg-1 weight day-1 ) were orally given rats for 7 days. Group 3 (PbA group): PbA (30 mg kg-1 ) was orally given to rats at 30 mg kg-1 PbA for 7 days. Group 4 (PbA +CH 25 group): 25 mg/kg CH was orally given to rats for 7 days. In addition, 30 mg kg-1 PbA was given orally to rats for 7 days after 30 minutes of the application of the CH. Group 5 (PbA+CH 50 group): 50 mg kg-1 CH was orally given to rats for 7 days. Furthermore, 30 mg kg-1 PbA was given orally to rats for 7 days after 30 minutes of the application of the CH. The rats were euthanized under mild sevoflurane anesthesia and tissue and blood samples were taken 24 hours after the last application (8th day). AST, ALT and ALP in serum, MDA, GSH and NO levels and SOD, CAT, GPx and arginase enzyme activities in liver tissue were measured. Serum ALT, AST and ALP levels increased in the PbA group when compared with the control, and this increase was partially corrected with CH. Liver MDA and NO levels increased in the PbA administered groups while antioxidant enzyme activities and GSH levels decreased. Although, CH administration decreased the increased MDA and NO levels, it increased the decreased antioxidant enzyme activities and GSH levels. Histologically, it was determined that CH decreased the necrosis, hydropic degenerations and mononuclear cell infiltrations seen in the livers of rats treated with PbA. As a result, CH is effective in the reducing PbA damages in the liver.
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İncele - MakaleYalnız künye2021Scopus SJRQ2Chrysin protects against testicular toxicity caused by lead acetate in rats with its antioxidant, anti-inflammatory, and antiapoptotic properties
Ileriturk, Mustafa; Benzer, Fulya; Aksu, Emrah Hicazi; Yildirim, Serkan; Kandemir, Fatih Mehmet; Dogan, Tuba; Dortbudak, Muhammet Bahaeddin; Genc, Aydin
In the present study, the protective effects of chrysin (CHR) against testicular damage caused by lead acetate (PbAc) were examined. In this way, 30 min after rats were given 25 and 50 mg/kg/b.w CHR orally for seven consecutive days, 30 mg/kg/b.w PbAc was administered orally. In biochemical analysis of testicular tissue, it was found that PbAc-reduced antioxidant parameters [glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT)], while it increased lipid peroxidation, inflammatory markers [nuclear factor kappa-B (NF-κB), cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE-2), and inducible nitric oxide synthase (iNOS)], and 8-hydroxy-2′-deoxyguanosine (8-OHdG). In the immunohistochemical examination, it was determined that PbAc increased the expression of tumor necrosis factor-α (TNF-α) and caspase-3. Accordingly, PbAc was found to cause a decrease in sperm motility and an increase in the percentage of dead sperm. However, it has been observed that CHR relieves oxidative stress due to its antioxidant properties, thus protecting against inflammation and apoptosis. It also allowed the CHR sperm parameters to return to control group levels. The results revealed that CHR could be a natural substance to be used in Pb-induced testicular toxicity. Practical applications: Lead (Pb) is an important environmental contaminant heavy metal. Pb is believed to reduce fertility in men. Oxidative stress plays a significant role in the damage caused by Pb to testicular tissue. CHR is an antioxidant substance that occurs naturally in various plants and has various pharmacological properties. In the present study, it was investigated whether CHR has a protective effect against testicular toxicity induced by PbAc. The results revealed that in rats, CHR protects the testicular tissue from PbAc toxicity by showing antioxidant, anti-inflammatory and anti-apoptotic effects, thus bringing sperm parameters closer to normal.
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İncele - MakaleYalnız künye2021Scopus SJRQ1Protective Effects of Chrysin Against Oxidative Stress and Inflammation Induced by Lead Acetate in Rat Kidneys: a Biochemical and Histopathological Approach
Kucukler, Sefa; Benzer, Fulya; Yildirim, Serkan; Gur, Cihan; Kandemir, Fatih Mehmet; Bengu, Aydin Sukru; Ayna, Adnan; Caglayan, Cuneyt; Dortbudak, Muhammet Bahaeddin
In this study, the protective effects of chrysin (CR) on lead acetate (PbAc)-induced renal toxicity in Sprague-Dawley rats were investigated with biochemical, histopathological, and immunohistochemical methods. In the study, rats were given orally at 30 mg/kg/body weight (BW) PbAc after CR of 25 and 50 mg/kg/BW was administered to them orally (a total of 7 administrations for 7 days). The results showed that CR reduced urea and creatinine levels by alleviating PbAc-induced kidney damage. It was determined that CR decreases PbAc-induced lipid peroxidation due to its antioxidant properties and increases catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, and glutathione (GSH) levels. It was also detected that CR protects DNA from the toxic effects of PbAc and reduces 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels. Biochemical and immunohistochemical findings demonstrated that CR had anti-inflammatory and antiapoptotic effects and reduced nuclear factor kappa-B (NF-κB), interleukin-33 (IL-33), prostaglandin-E2 (PGE-2), tumor necrosis factor-α (TNF-α), p53 levels, and the activities of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), which were increased with PbAc administration. Moreover, CR was found to increase the levels of aquaporin-1 (AQP-1) and nephrine in PbAc-induced kidney tissue. CR decreased the contents of lead (Pb), zinc (Zn), iron (Fe), sodium (Na), and copper (Cu) and increased those of potassium (K) calcium (Ca) in renal tissue. These results indicated that CR considerably alleviates kidney toxicity caused by PbAc.
AtıfScopus136WoS118OpenAlex145İncele