Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2019Scopus SJRQ4Investigation of relaxation times in 5-fluorouracil and human serum albumin mixtures
Korunur, Sibel; Zengin, Bilgin; Yilmaz, Ali
Background: Human serum albumin (HSA) is often selected as a subject of any study because albumin is the most abundant protein in human blood plasma. NMR is recognized as a valuable method to determine the structure of proteins-ligand and protein-drug complexes. Objective - Aim of the study: In this study, protein drug interactions were investigated using 5-Fluorouracil anticancer drug and human serum albumin protein. Materials and methods: In this context 400 MHz NMR spectrometry was used and NMR relaxation rates in drug-albumin complex were investigated with respect to increase albumin concentration and increase in 5-Fluoro-uracil (5-FU)-albumin solution temperature. Results: The results of this study indicated that 5-FU had a weak association with albumin, and it easily dissociated from the protein to which it was attached. Conclusion: The obtained results also gave us useful information about molecular dynamics of drug-albumin interactions.
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İncele - MakaleYalnız künye2013Scopus SJRQ3Elimination of Radiation Damping Effects from the NMR Relaxation Curves of H2O/D2O Mixtures Containing Protein and Ions
Zengin, Bilgin; Koylu, Mehmet Zafer; Korunur, Sibel; Yilmaz, Ali
In this work the T-1 and T-2 curves of H2O/D2O mixtures were obtained for several fractions of H2O and also for different amounts of albumin and manganese. The experiments were carried out with a 400 MHz proton NMR spectrometer. The T-1 and the T-2 curves were determined by the inversion recovery (IR) and Carr-Purcell-Meiboom-Gill (CPMG) sequences, respectively. The relaxation times in residual water and in a mixture with 0.05 ml H2O were found to be reliable either in the presence or in the absence of albumin. The mixtures containing 0.1 ml or higher H2O suffer from radiation damping (RD). RD was removed gradually by addition of albumin or manganese. RD in the protein solution with 0.1 ml H2O was removed by adding 1 mu g of manganese to one ml of solution. The data suggest that the relaxation times in H2O/D2O mixtures are measurable by conventional methods upon the addition of appropriate amounts of manganese and albumin.
AtıfWoS2İncele - MakaleAçık Erişim2010Scopus SJRQ2The Nature of the Crustal Structure of the Eastern Anatolian Plateau, Turkey
Yilmaz, Ali; Yilmaz, Huseyin; Kaya, Cemal; Boztug, Durmus
The Eastern Anatolian Plateau (EAP) of Turkey, with an elevation ranging from 1700 to 2000 m, is located between the Eastern Pontide Arc to the north and the Arabian Platform to the south. In this region, pre-Maastrichtian tectonic units representing the crust crop out in only a few localities. As they are covered by Maastrichtian-Quaternary rock units, it is difficult to study the nature and mutual relationships of these pre-Maastrichtian tectonic units. The palaeotectonic units of the EAP comprise two different levels in the present study: (1) The lower level consists of platform-type carbonates and their metamorphic equivalents. These units may represent the Taurus Platform and its metamorphic equivalents. (2) The upper level consists of an ophiolitic-melange prism which is made up mainly of oceanic crust; the prism comprises a complex of ophiolite, ophiolitic melange, and fore-arc deposits. This upper unit represents a subduction-accretion prism and may have originated partly from the North Anatolian Suture to the north, and partly from the South-eastern Anatolian Suture to the south. Continental crustal rocks were thrust over by the ophiolitic melange prism; thus outcrops of them are scarce in the region as they are exposed in tectonic windows through the ophiolitic thrust sheets. The pre-Maastrichtian tectonic units of the EAP are blanketed by Maastrichtian to Quaternary volcanic and sedimentary rock units; these sequences include successive transgressive and regressive intervals and overlie the palaeotectonic units along a pronounced unconformity. Olistostromal units are abundant in the Eocene sedimentary units and were derived from the ophiolites and ophiolitic melange. The Maastrichtian-Quaternary cover is made up of collisional and post-collisional deposits across the whole region. Although the EAP has been experiencing considerable N-S compression, it has not been affected by significant crustal thickening because of the strike-slip tectonic regime that is dominant in the region. (C) 2010 Lavoisier SAS. All rights reserved
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