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- MakaleYalnız künye2025Scopus SJRQ4The profile of fatty acids in the gametes of brook trout (Salvelinus fontinalis) ofduring the spawning period under culture conditions
Kocabas, Mehmet; Kutluyer-Kocabas, Filiz; Basçinar, Nadir; Özçiçek, Esin; Kirmizikaya-Özmen, Gorkem; Yilmaz, Okkes
The fertilization and subsequently embryo and larvae development are linked to gamete quality and FA (fatty acid) content is one of the specific factors affecting egg or sperm quality. Hence, FA profile of gametes (egg and sperm) was documented in brook trout (Salvelinus fontinalis) under cultivation. FAs were detected and measured using gas chromatography. These results indicated that a total of 26 and 22 FAs were determined in egg and sperm, respectively. The levels of PUFAs were determined in nearly identical quantities in both egg (47.57%) and sperm (47.53%). DHA (Docosahexaenoic acid), LA (Linoleic acid), EPA (Eicosapentaenoic acid) and ARA (Arachidonic acid) were predominant PUFAs for egg and sperm. Interestingly, vaccenic acid (C18:1 n-11) (24.07%) was major FAs in egg. In conclusion, the analysis of fatty acids in broodstock gametes contributes valuable insights into the dietary requirements necessary for effective aquaculture breeding programs.
- MakaleAçık Erişim2024Investigation of Flower, Leaf, and Stem Parts of Three Gundelia Species Growth in the Eastern Anatolia Region of Türkiye from a Biochemical Perspective
Yilmaz, Fatih; Türkoglu, Ismail; Kirmizikaya Özmen, Gorkem; Yüce Babacan, Ebru; Yilmaz, Okkes
13 species of Gundelia species plants are endemic in T & uuml;rkiye and are frequently used by the public for both nutrition and medicinal objectives. In this study, three parts (flower, leaf, and stem) of three species of Gundelia ( G.dersim , G.glabra, and G.munzurensis) were biochemically investigated. DPPH and ABTS analyses for antioxidant activity were performed on methanol extracts of plant parts. As a result of both analyses in parallel, the flower part of G.munzurensis had the highest %Inhibition value (DPPH:91.85 +/- 0.78; ABTS:97.38 +/- 0) (P<0.001). In total phenolic (29.09 mg GAE/g) and total flavonoid (5.59 mg CE/g) measurements, the highest amount was found statistically quite significant (P<0.001) in the leaf part of G.munzurensis. Gallic, vanillic, ferulic, romantic, and cinnamic acids, which are phenolic acids and of the flavonoids, rutin, catechin, naringin, and naringenin, were determined in all plant parts. Vitamin D3, alpha-tocopherol, ergosterol, stigmasterol, and beta-sterol were detected in parts of all plant species. While palmitic (16:0), palmitoleic (16:1), stearic (18:0), oleic (18:1), linoleic (18:2), and linolenic (18:3) fatty acids as the main fatty acids were detected in all parts of the three Gundelia species, lauric (12:0), tridecanoic (13:0), myristic (14:0), pentadecanoic (15:0), cis-10-pentadecanoic (15:1), heptadecanoic (17:0), cis-10heptadecanoic (17:1), and eicosanoid (21:0) acids were also identified at different concentrations. Considering the biochemical properties of Gundelia species, it shows that they can be consumed as food and also used pharmacologically.
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İncele - MakaleAçık Erişim2021The antioxidant and antimicrobial capacities of phenolic profiles of some Salvia L. Seeds grown in Turkey
Emre, Irfan; Kursat, Murat; Kirbag, Sevda; Erecevit, Pinar; Emre, Mustafa Yunus; Yilmaz, Okkes; Civelek, Semsettin
The aim of current study is to show phenolics, antioxidant capacities and antimicrobial activities of seeds of five Salvia L. (S. frigida Boiss., S. candidissima subsp. candidissima Vahl., S. virgata Jacq., S. verticillata L. var. verticillata and S. russellii Benth.) taxa grown in Turkey. The flavonoid and phenolic acid contents were measured by using HPLC whilst the antioxidant capacities were determined by using different methods. In addition, agar well diffusion method was used to determine the antimicrobial activities of Salvia species in this study. It was found that S. frigida, S. verticillata var. verticillata and S. russellii have the highest catechin contents and S. frigida and S. verticillata var. verticillata have high rosmarinic acid while S. frigida, S. candidissima subsp. candidissima and S. verticillata var. verticillata have high vanilic acid. Also, it was determined that S. frigida and S. verticillata var. verticillata have high DPPH radical scavenging activities in 150 and 250 µL while S. frigida and S. verticillata var. verticillata have highest ABTS radical scavenging activity in all concentrations apart from 25 µL for S. frigida. Furthermore, S. frigida and S. verticillata var. verticillata have high total phenolic contents. On the other hand, Salvia species have similar lipid peroxidation inhibitions. However, the metal chelating activities of Salvia species are different. And also, it was demonstrated that Salvia taxa have antimicrobial activity.
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İncele - MakaleYalnız künye2020Scopus SJRQ4The protective effects of golden berry (Physalis peruviana L.) extract against to the oxidative and destructive effects of type i diabetes in rats
Erman, Fazilet; Kaya, Tubay; Aydin, Sevinc; Erman, Orhan; Yilmaz, Okkes
Type I diabetes is known as insulin-dependent diabetes mellitus, and the most important factor playing role in its formation is the genetic predispo- sition. Physalis peruviana L. (Goldenberry) is a plant, which has strong antioxidant properties, from Solanaceae family and, because of its fructose con- tent, plays regulatory role in blood glucose level of diabetics. In this study, it has been investigated the effects of goldenberry on the malondialdehyde (MDA), reduced glutathione (GSH), total protein, vitamins A, D, E, and K, and cholesterol levels which are the indicators of antioxidant defense and the oxidative damages in serum, muscle, kidney, liver and brain tissues of the rats, on which type I diabetes was induced by STZ. According to the obtained results, among the rats with STZ-induced type I diabetes, it was ob- served that the level of glutathione (GSH) increased and the level of malondialdehyde (MDA) decreased in all of the tissues of rats given goldenberry extract, while significant decreases were determined in the levels of vitamins K-1, K-2, D-2 and D-3 in all of tissues (except serum), and there were also decreases in the level of α-tocopherol and cholesterol in tis- sues of muscle, liver and kidney. In this study, it was shown that the goldenberry decreased the destructive effects of type-I diabetes by decreasing the serum glucose and lipid peroxida- tion and increasing the level of glutathione. It is believed that the obtained results would be used in fol- low-up of diabetic patients and in early diagnosis of the disease in future.
AtıfScopus4İncele - MakaleAçık Erişim2014The investigation of some bioactive compounds and antioxidant properties of hawthorn (Crataegus monogyna subsp. monogyna Jacq)
Keser, Serhat; Celik, Sait; Turkoglu, Semra; Yilmaz, Okkes; Turkoglu, Ismail
The antioxidant and pharmacological effects of hawthorn have mainly been attributed to the polyphenolic contents. The aim of this research is to determine some bioactive compounds and antioxidant properties of hawthorn aqueous and ethanol extracts of leaves, flowers, and ripened fruits. Materials and Methods: For this purpose, antioxidant activities of extracts were assessed on DPPH center dot, ABTS center dot +, superoxide scavenging, reducing power and ferrous metal chelating activity assays and phenolic content of extracts was determined by Folin-Cioacalteu's reagent. Results: The flavonoids including rutin, apigenin, myricetin, quercetin, naringenin and kaempferol, were identified by high- performance liquid chromatography in the hawthorn extract. Conclusion: It was observed the aqueous and ethanol extracts of Crataegus monogyna subsp. monogyna fruits showed the highest activity in reducing power and metal chelating activity assays. In addition, it was determined that the aqueous flower extract showed higher flavonoid content than aqueous leaves extract. The antioxidant and pharmacological effects of hawthorn have mainly been attributed to the polyphenolic contents.
AtıfWoS47OpenAlex58İncele - MakaleAçık Erişim2013Scopus SJRQ3Determination of phytochemical characteristics of the extract of Zea mays with Saccharomyces boulardii
Erecevit, Pinar; Kirbag, Sevda; Yilmaz, Okkes
Fatty acid, vitamin, phytosterol, flavonoid, and resveratrol contents and antimicrobial activities of Zea mays L. (corn) extracts treated with Saccharomyces boulardii were determined and compared. The total fatty acid, vitamin, and phytosterol contents in corn extract were at certain levels. It was detected that total fatty acid levels and vitamin contents of corn extracts prepared with Saccharomyces boulardii increased; however, flavonoid contents decreased at different rates. In addition to this, it was observed that naringin, resveratrol, and myricetin flavonoids were present in the extracts that contained S. boulardii. In the study, it was noticed that Z. mays L. (corn) had antioxidant and antimicrobial activities at different rates. It was observed that S. boulardii developing in extracts obtained from plants affected bioactive compounds at different rates.
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